RESUMO
We studied the effect of reduced tryptophan hydroxylase (TPH) activity and short daylight exposure on the behavior and the 5-HT system of the brain in D. rerio. Male and female D. rerio were exposed for 30 days to standard (12:12 h light:dark) and short (4:20 h light:dark) photoperiods in the presence or absence of TPH inhibitor (p-chlorophenylalanine, pCPA, 5 mg/liter). On day 31, the fish behavior in the "novel tank diving" test, their sex and body weight were determined, and the levels of pCPA, 5-HT, and its metabolite 5-HIAA were measured by HPLC; the levels of the key genes encoding metabolism enzymes (Tph1a, Tph1b, Tph2, and Mao) and receptors of 5-HT (Htr1aa, Htr2aa) were assessed by real-time PCR with reverse transcription. The short daylight exposure caused masculinization of females, reduced body weight, and motor activity in the "novel tank diving" test, but did not affect the 5-HT system of the brain. Long-term pCPA treatment had no effect on sex and body weight, significantly reduced the 5-HIAA level, but increased Tph1a and Tph2 gene expression in the brain. No effects of the interaction of short daylight and pCPA exposure on the sex, body weight, behavior, and 5-HT system of the brain were found. Thus, a moderate decrease in TPH activity cannot potentiate the negative effects of short daylight exposure on the sex, body weight, behavior, and 5-HT system of D. rerio.
Assuntos
Serotonina , Peixe-Zebra , Animais , Masculino , Feminino , Serotonina/farmacologia , Serotonina/metabolismo , Peixe-Zebra/metabolismo , Triptofano Hidroxilase/genética , Triptofano Hidroxilase/metabolismo , Ácido Hidroxi-Indolacético/metabolismo , Encéfalo/metabolismo , Fenclonina/farmacologia , Fenclonina/metabolismo , Peso CorporalRESUMO
Reduced daylight duration causes the development of seasonal affective disorder (SAD; depression-like disorders characterized by depressed mood, apathy, bulimia, and weight gain) in sensitive individuals. Neurotransmitter serotonin (5-HT) is involved in the mechanism of SAD. Zebrafish (D. rerio) is a promising model for translational studies. We studied changes in the behavior, content of 5-HT and its major metabolite 5-hydroxyindoleacetic acid (5-HIAA), and the expression of genes encoding the key enzymes of 5-HT metabolism, tryptophan hydroxylases TPH1A, TPH1B, TPH2, monoamine oxidase (MAO), 5-HT transporter, and 5-HT1A and 5-HT2A receptors in the brain of zebrafish reared for 60 days under short (04:20 h) compared to those reared at normal (12:12 h) photoperiod. Exposure to short photoperiod decreased locomotor activity in the novel tank diving test, increased the level 5-HIAA, and reduced the level of Mao gene mRNA, but did not affect the level of 5-HT and expression of Tph1a, Tph1b, Tph2, Slc6a4a (transporter), Htr1aa, and Htr2aa (receptors) genes. Thus, zebrafish can be used as a promising model to study the involvement of 5-HT in the SAD mechanism.
Assuntos
Serotonina , Peixe-Zebra , Animais , Encéfalo/metabolismo , Ácido Hidroxi-Indolacético/metabolismo , Monoaminoxidase/genética , Monoaminoxidase/metabolismo , Fotoperíodo , Serotonina/metabolismo , Peixe-Zebra/genética , Peixe-Zebra/metabolismoRESUMO
Effects of acute treatment with antidepressant drugs, imipramine and citalopram, on behavior and activity of striatal-enriched tyrosine protein phosphatase (STEP) in the whole brain of zebrafish Danio rerio were studied. Mature zebrafish were exposed for 3 h to water (control) or to solutions of 0.25, 0.5, or 1 mg/liter of imipramine or citalopram, and then their behavior was studied in novel tank test. STEP activity was assayed in the brain of animals by the difference between the rates of transformation of p-nitrophenyl phosphate to 4-nitrophenol in the absence or presence of a selective STEP inhibitor. In novel tank test, imipramine and citalopram reduced locomotor activity and increased freezing time; at this, imipramine increased the total time spent in top of the tank. Citalopram (all concentrations) and imipramine (0.5 and 1 mg/liter) increased STEP activity in zebrafish brain.