RESUMO
Current knowledge of the pathogenic hantavirus indicates that wild rodents are its primary natural reservoir. Specific primers to detect the presence of viral genomes were developed using an SYBR-Green-based real-time RT-PCR protocol. One hundred sixty-four rodents native to the Atlantic Forest biome were captured in São Paulo State, Brazil, and their tissues were tested. The presence of hantavirus RNA was detected in sixteen rodents: three specimens of Akodon montensis, three of Akodon cursor, two of Necromys lasiurus, one of Juliomys sp., one of Thaptomys nigrita, five of Oligoryzomys nigripes, and one of Oryzomys sp. This SYBR Green real-time RT-PCR method for detection of hantavirus may be useful for surveying hantaviruses in Brazil.
Assuntos
Reservatórios de Doenças/virologia , Orthohantavírus/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Roedores/virologia , Animais , Sequência de Bases , Benzotiazóis , Brasil , Diaminas , Orthohantavírus/classificação , Orthohantavírus/genética , Dados de Sequência Molecular , Compostos Orgânicos/química , Filogenia , Quinolinas , Reação em Cadeia da Polimerase Via Transcriptase Reversa/instrumentaçãoRESUMO
This study describes infection of microsporidia in the natural fauna of small wildlife animals in an area of deforestation for a water reservoir construction in the state of São Paulo, Brazil. It was focused on marsupials, small rodents, frogs, primates, bats, and others mammals taken from this area to access whether they may represent environmental sources of these zoonoses. From all captured animals, microsporidia spores were observed in the feces of bats, small rodents, and marsupials. This study emphasises the importance of wild animals, particularly small mammals as potential sources of microsporidia to human and animal populations from deforested areas.
