Assuntos
Cartilagem Articular/anatomia & histologia , Processamento de Imagem Assistida por Computador/métodos , Articulação do Joelho/anatomia & histologia , Imageamento por Ressonância Magnética/métodos , Adulto , Cartilagem Articular/lesões , Fêmur/anatomia & histologia , Fêmur/patologia , Humanos , Aumento da Imagem/métodos , Traumatismos do Joelho/diagnóstico , Traumatismos do Joelho/patologia , Patela/anatomia & histologia , Patela/patologiaRESUMO
A two turn saddle shaped surface coil receiver was developed that allowed high resolution magnetic resonance imaging of the rat spinal cord. This is particularly important in laboratory animals where central nervous system regions of interest are relatively small. A continuous copper wire 1.5 mm in diameter was wound into two turns 28 mm in diameter. The saddle shape of the second turn improved the homogeneity of the signal within the region of interest and maintained sufficient field of view and depth of penetration. The quality factor (Q) for the surface coil was Q = 199 unloaded, and Q = 60 loaded. Using this surface coil with a GE CSI II 2.0 Tesla small bore magnet, spin echo T1 (TR = 500 msec, TE = 25 msec) and T2 (TR = 2000 msec, TE = 100 msec) weighted images were obtained in cross section, using 2 mm slice thickness with 2 excitations per phase encoding step. A sagittal gradient echo (rapid scan, TR = 85 msec, TE = 10 msec) was used to document reestablishment of vascular flow following ischemia. Spinal cord ischemia was induced by 14 minute temporary occlusion of spinal cord blood supply. MRI was performed at 18 hours following ischemia. There was a 1.4 fold increase in T2 image intensity in ischemic rat spinal cord (n = 4), consistent with edema formation, compared to normal rat spinal cord (n = 4). Preliminary studies show that similar high resolution images can be performed on the rat brain. This technique uses standard MRI equipment and the surface coil is made from inexpensive readily available materials. There are various animal models of cerebral and spinal cord injury that would benefit from improved high resolution MRI. This coil design may have application in larger animal models and the clinical setting.
Assuntos
Água Corporal/metabolismo , Isquemia/patologia , Imageamento por Ressonância Magnética/métodos , Medula Espinal/patologia , Animais , Isquemia/metabolismo , Masculino , Ratos , Ratos Sprague-Dawley , Medula Espinal/irrigação sanguíneaRESUMO
PURPOSE: To determine, with hydrodynamic experiments, the true cause of pulsus tardus, a Doppler waveform alteration that often occurs distal to an arterial stenosis. MATERIALS AND METHODS: A flow phantom was constructed with a pulsatile pump, interchangeable stenoses of varying degree, and interchangeable poststenotic segments of tubing with various degrees of compliance. With the transstenotic pressure drop held constant for each stenosis, Doppler waveforms were obtained before and after stenosis, while the degree of poststenotic vessel compliance was varied. RESULTS: The degree of pulsus tardus increased as the compliance of the poststenotic segment of vessel increased, independent of the transstenotic pressure drop. CONCLUSION: Poststenotic pulsus tardus is caused by the compliance of the poststenotic vessel wall in conjunction with the stenosis, which produces the tardus effect by damping the high-frequency components of the arterial waveform. This information will allow prediction of conditions that may produce false-positive or false-negative results when the tardus phenomenon is used to predict substantial upstream stenosis.
Assuntos
Pulso Arterial/fisiologia , Obstrução da Artéria Renal/diagnóstico por imagem , Artéria Renal/diagnóstico por imagem , Velocidade do Fluxo Sanguíneo/fisiologia , Reações Falso-Negativas , Reações Falso-Positivas , Humanos , Modelos Cardiovasculares , Modelos Estruturais , Artéria Renal/fisiopatologia , Obstrução da Artéria Renal/fisiopatologia , Ultrassom , Ultrassonografia , Resistência Vascular/fisiologiaRESUMO
Sixteen anesthetized New Zealand white rabbits were subjected to thoracotomy, and a reversible snare occluder was attached around a large branch of the left circumflex coronary artery. A 1.3 cm. diameter nuclear magnetic resonance (NMR) surface coil was placed adjacent to the myocardium perfused by this vessel. The animals were divided into two groups of eight animals each, treatment and control. The rabbits were studied using a 2.0 T magnetic resonance (MR) spectrometer, and baseline spectra were acquired. The treatment animals then received intravenous propranolol (1.5 mg/kg) and the control animals received an equal volume of saline. Spectra were then acquired during a 20-minute occlusion period and during subsequent reperfusion. Animals in both groups showed expected decreases in phosphocreatine and adenosine triphosphate and an increase in inorganic phosphate during occlusion; these changes reverted toward baseline values with reperfusion. There were no significant differences between the two groups. The myocardium became acidotic during occlusion in both groups, but significantly more so in the control animals: during the first 10 minutes of occlusion pH was 7.30 +/- 0.41 in the treatment group versus 6.55 +/- 0.24 for controls (p = 0.0005). During the second 10 minutes of occlusion pH was 7.05 +/- 0.65 in the treatment group versus 6.24 +/- 0.25 in controls (p = 0.0053). We conclude that attenuation of intracellular acidosis by propranolol during myocardial ischemia was evident by MR spectroscopy in this animal model.
Assuntos
Doença das Coronárias/metabolismo , Imageamento por Ressonância Magnética , Reperfusão Miocárdica , Miocárdio/metabolismo , Propranolol/farmacologia , Animais , Doença das Coronárias/diagnóstico , Doença das Coronárias/fisiopatologia , Frequência Cardíaca/efeitos dos fármacos , Concentração de Íons de Hidrogênio , CoelhosRESUMO
Diltiazem may provide a protective effect to ischemic and reperfused myocardium through preservation of high-energy phosphate metabolism. To test this hypothesis, rabbits had a 1.3 cm solenoidal coil placed over the myocardium to be rendered ischemic. Data were acquired with a 22 cm bore nuclear magnetic resonance spectrometer at 2.0 T. Animals were treated with diltiazem (200 micrograms/kg intravenous bolus of drug followed by a 15 micrograms/kg/min continuous intravenous infusion, n = 10) or by an equal volume of saline (n = 6). The left circumflex artery was occluded and reperfused using a reversible snare while electrocardiogram-gated spectra were accumulated. Levels of phosphocreatine were decreased during occlusion in both groups; however, this decrease was attenuated in the diltiazem treated animals compared to control (in relative percent area: 7.8 +/- 1.0 to 2.5 +/- 0.5, p less than 0.01). Levels of phosphocreatine promptly returned to baseline following reperfusion and there was no difference between the two groups. The inorganic phosphate metabolites of high-energy phosphate consumption increased with occlusion, though more so in the control group compared with the diltiazem-treated rabbits (in relative percent area: 72.5 +/- 0.9 to 55.4 +/- 1.3, p less than 0.01). With reperfusion, levels of inorganic phosphates returned toward baseline in both groups; however, the diltiazem group had a more complete recovery relative to control (in relative percent area: 38.8 +/- 2.1 to 47.6 +/- 2.7, p less than 0.05). Levels of adenosine triphosphate decreased in both groups relative to baseline; however, the amount of decrease was similar in the two groups. With reperfusion there was a definite though incomplete recovery of levels of adenosine triphosphate in the diltiazem-treated group (in relative percent area: 10.7 +/- 1.0 at occlusion, 12.3 +/- 0.4 during reperfusion, p less than 0.05), but in the control group levels of adenosine triphosphate remained depressed (in relative percent area: 9.8 +/- 0.6 at occlusion, 9.8 +/- 0.8 during reperfusion, p = NS). During ischemia there was a trend toward attenuation of intracellular acidosis in the diltiazem group; however, this trend did not reach statistical significance. These data indicate that diltiazem provides a protective effect on myocardial high-energy phosphate metabolism during regional ischemia and reperfusion in the intact animal.
Assuntos
Doença das Coronárias/metabolismo , Diltiazem/farmacologia , Espectroscopia de Ressonância Magnética , Reperfusão Miocárdica , Fosfatos/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Fosfocreatina/metabolismo , Fósforo , CoelhosRESUMO
The objective of this work is refinement of an MR angiography technique via postprocessing removal of phase errors which inhibit static signal subtraction. Projective views of the object are obtained using interleaved flow-compensated and noncompensated gradient waveforms. Complex subtraction of data sets is required since a projection dephase pulse is used for static signal suppression. This renders the difference image susceptible to systematic phase errors which are modeled as a smoothly varying multiplicative phase function. The error function is estimated by comparison of heavily spatial filtered renditions of the object acquired without projection dephasing in order to minimize influence of flow. The phase correction is then applied to high-resolution data sets collected with projection dephasing to enhance flow sensitivity. The technique is demonstrated by improvement of MR angiograms of rats acquired on a 2-T, 31-cm-bore system.
Assuntos
Imageamento por Ressonância Magnética/métodos , Animais , Velocidade do Fluxo Sanguíneo , Pescoço/irrigação sanguínea , Ratos , Técnica de SubtraçãoRESUMO
Few studies have examined metabolic consequences of coronary occlusion and reperfusion using phosphorus31 nuclear magnetic resonance (31P-NMR) in an intact animal model. Accordingly, we developed a model to study serial changes in myocardial metabolism in the intact open-chest rabbit. Ten animals underwent 20 +/- 2 minutes of regional coronary occlusion and 60 +/- 10 minutes of reperfusion followed by reocclusion. Cardiac-gated 31P-NMR spectra were obtained with a regional surface coil over the ischemic area during baseline, occlusion, reperfusion, and reocclusion conditions. Phosphocreatine fell with both the initial and second ischemic insults to 65% +/- 5% of baseline for the first occlusion (p less than 0.01) and tended to decrease to 89% +/- 8% of baseline for the second occlusion (p = 0.07), with normal levels reattained in the intervening period of reperfusion (99% +/- 5% of baseline, p = NS). Concordant inverse changes were seen with inorganic phosphates. At occlusion levels of inorganic phosphates were 135% +/- 10% of baseline (p less than 0.05) and 139% +/- 10% of baseline at reocclusion (p less than 0.05). Levels of adenosine triphosphate decreased during occlusion to 78% +/- 9% of baseline and were significantly lower than baseline during the second occlusion (75% +/- 5% of baseline, p less than 0.01). The ratio of phosphocreatine to inorganic phosphates, when compared with values at baseline, decreased at occlusion (49.6% +/- 4.7% of baseline, p less than 0.01) and at reocclusion (64.7% +/- 4.9% of baseline, p less than 0.01), with a normal ratio reattained in the intervening period of reperfusion (93.3% +/- 3.1% of baseline, p = NS). We conclude that reperfusion restores levels of phosphocreatine and adenosine triphosphate while returning levels of inorganic phosphates to baseline. Deleterious changes in high-energy phosphate metabolism are not potentiated by reocclusion in this model. 31P-NMR spectroscopy holds promise as a technique to noninvasively monitor intracellular biochemical processes serially during various interventions in the intact animal model.
Assuntos
Doença das Coronárias/metabolismo , Espectroscopia de Ressonância Magnética , Reperfusão Miocárdica , Miocárdio/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Doença das Coronárias/fisiopatologia , Fosfatos/metabolismo , Fosfocreatina/metabolismo , CoelhosRESUMO
Magnetic resonance (MR) imaging has given mixed results in the detection of renal masses. To identify the reasons for this and to determine the optimal pulse sequences for evaluating renal tumors, the authors imaged 12 primary renal tumors in vivo and 17 in vitro at 0.35 T. Histopathologic findings for each specimen were closely correlated with the MR images. Four of seven solid tumors imaged in vivo were isointense with surrounding normal renal parenchyma at all pulse sequences. The other three tumors were hyperintense in vivo at T2-weighted sequences. At heavily T2-weighted sequences eight solid tumors were hyperintense in vitro and four were hypointense. There was no correlation between signal intensity and specific tissue type or histologic pattern for solid tumors. The five cystic tumors were well seen both in vivo and in vitro on T2-weighted images. However, the signal intensity of the cyst fluid was an unreliable indicator of benignancy. SE MR imaging at 0.35 T has significant limitations in the detection of solid renal masses.
Assuntos
Carcinoma de Células Renais/diagnóstico , Doenças Renais Císticas/diagnóstico , Neoplasias Renais/diagnóstico , Rim/patologia , Imageamento por Ressonância Magnética/métodos , HumanosRESUMO
A small-aperture, prototype ultrasonic imaging system, typical of the size necessary to be embedded in a catheter, was developed to evaluate the feasibility of intravascular ultrasonic imaging catheters (UICs). The evaluation included in vitro imaging of postmortem samples of human femoral and iliac arteries with the UIC prototype and a high-resolution magnetic resonance (MR) proton imager. Excellent correlations between results from the UIC images, MR images, and tissue specimens were demonstrated. Although the current prototype is too large to be useful in examination of smaller vessels such as coronary arteries, the signal-to-noise ratio and resolution obtained indicate that imaging with intravascular UICs is feasible and may have significant diagnostic value.