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1.
Braz J Microbiol ; 54(3): 2393-2401, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37407882

RESUMO

Antimicrobial resistance is a threat to public health. The emergence of antibiotic-resistant Staphylococcus aureus represents a priority for the implementation of preventive measures. The objective was to isolate S. aureus in humans, animals, and animal health care environment, and to characterize the genotypic and phenotypic profile of antimicrobial resistance in these isolates. We isolated S. aureus from staff, animals, and environment of a veterinary hospital, and identified their antimicrobial resistance profiles. Samples were collected from 20 humans, 13 animals, 14 surfaces, 8 mobile phones, and 7 veterinarians' stethoscopes by using sterile swabs. S. aureus was isolated by culturing on mannitol salt agar and preliminary identification was done by Gram staining and catalase test. Subsequently, a polymerase chain reaction was performed for species confirmation and investigating their antimicrobial-resistant genotypic profiles. Phenotypic profiles of resistant isolates were determined using the disk-diffusion technique. Ten S. aureus isolates were recovered from 5/20 humans (25%), it was also recovered from 2/13 animals (15.38%), including 1 dog and 1 cat, and from 1/14 of surfaces (7.14%). The oxacillin-susceptible mecA-positive Staphylococcus aureus phenotype was identified in a feline. Most of the isolates carried at least two resistance genes of different antimicrobial classes, with 90% (9/10) presenting the gene blaZ, with 10% (1/10) presenting the gene mecA, 20% (2/10) presenting tet38, 10% (1/10) presenting tetM, 90% (9/10) presenting norA, 50% (5/10) presenting norC, 10% (1/10) presenting ermA, and 60% (6/10) presenting ermB. In antibiograms, resistance to penicillin was identified in all the isolates, resistance to erythromycin was identified in 80% (8/10), and all the isolate's resistance to erythromycin presented erythromycin-induced resistance to clindamycin. Antimicrobial resistance in the veterinary hospital requires attention due to the risk of interspecies transmission, gene transfer between bacteria that colonize companion animals and humans and, can make antimicrobial therapy difficult.


Assuntos
Anti-Infecciosos , Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Humanos , Gatos , Animais , Cães , Staphylococcus aureus , Staphylococcus aureus Resistente à Meticilina/genética , Brasil , Hospitais Veterinários , Antibacterianos/farmacologia , Infecções Estafilocócicas/microbiologia , Eritromicina/farmacologia , Testes de Sensibilidade Microbiana
2.
Rev Soc Bras Med Trop ; 52: e20180440, 2019 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-30994810

RESUMO

INTRODUCTION: The present study aimed to assess the seroprevalence and spatial distribution of Neospora caninum and Toxoplasma gondii in dogs. METHODS: Blood samples (n = 241) were collected and analyzed for the presence of anti-N. caninum and anti-T. gondii antibodies. The spatial distribution was evaluated using kernel density estimation (KDE). RESULTS: Anti-N. caninum and anti-T. gondii antibodies were detected in 24.06% (58/241) and 9.54% (23/241) of samples, respectively. A heterogeneous spatial distribution of positive dogs was observed across the city. CONCLUSIONS: These data are pivotal for better understanding the dynamics of infection caused by these protozoa in the canine population.


Assuntos
Anticorpos Antiprotozoários/sangue , Coccidiose/veterinária , Doenças do Cão/epidemiologia , Neospora/imunologia , Toxoplasma/imunologia , Toxoplasmose Animal/epidemiologia , Animais , Brasil/epidemiologia , Coccidiose/diagnóstico , Coccidiose/epidemiologia , Doenças do Cão/diagnóstico , Cães , Feminino , Masculino , Fatores de Risco , Estudos Soroepidemiológicos , Análise Espacial , Toxoplasmose Animal/diagnóstico , População Urbana
3.
Rev. Soc. Bras. Med. Trop ; Rev. Soc. Bras. Med. Trop;52: e20180440, 2019. graf
Artigo em Inglês | LILACS | ID: biblio-1041558

RESUMO

Abstract INTRODUCTION: The present study aimed to assess the seroprevalence and spatial distribution of Neospora caninum and Toxoplasma gondii in dogs. METHODS: Blood samples (n = 241) were collected and analyzed for the presence of anti-N. caninum and anti-T. gondii antibodies. The spatial distribution was evaluated using kernel density estimation (KDE). RESULTS Anti-N. caninum and anti-T. gondii antibodies were detected in 24.06% (58/241) and 9.54% (23/241) of samples, respectively. A heterogeneous spatial distribution of positive dogs was observed across the city. CONCLUSIONS These data are pivotal for better understanding the dynamics of infection caused by these protozoa in the canine population.


Assuntos
Humanos , Animais , Masculino , Feminino , Cães , Toxoplasma/imunologia , Anticorpos Antiprotozoários/sangue , Toxoplasmose Animal/epidemiologia , Coccidiose/veterinária , Neospora/imunologia , Doenças do Cão/epidemiologia , População Urbana , Brasil/epidemiologia , Estudos Soroepidemiológicos , Toxoplasmose Animal/diagnóstico , Fatores de Risco , Coccidiose/diagnóstico , Coccidiose/epidemiologia , Doenças do Cão/diagnóstico , Análise Espacial
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