RESUMO
The authors describe an analog biophotometer for the implementation of a method of the biological reference point that enables the comparison of the photometric diagnosis data for biological objects having different optic characteristics. The analog biophotometer can be used for superficial tissues of biological objects as well as for the diagnosis of not easily accessible areas, which is carried out by means of a fiber optical sensor contained in the apparatus.
Assuntos
Óptica e Fotônica , Fotometria , Tecnologia de Fibra Óptica , Humanos , Fotometria/métodosRESUMO
To eliminate the masking effect of the background luminescence of endogenic substances in biological tissues and to increase the luminescence contrast values of malignant tumours, sarcoma-implanted mice were administered with ytterbium porphyrins instead of free porphyrin radicals. This was followed by in vivo luminescence localization of tumours using fibre-laser spectrofluorometry. The luminescence contrast values obtained reached 10-45 which is evidently not a limit.
Assuntos
Porfirinas , Sarcoma Experimental/diagnóstico , Animais , Medições Luminescentes , Camundongos , Camundongos Endogâmicos C57BL , Porfirinas/farmacocinética , Sarcoma Experimental/metabolismo , Espectrometria de Fluorescência , Espectrofotometria Infravermelho , ItérbioRESUMO
The biophysical bases of photoplethysmography in the reflected light were investigated by means of simulating the biological object-photoplethysmographic transducer system. The parameters of the system, exerting the most powerful influence on the photoplethysmographic readings, were defined. The program for selecting an optimal design of the fiber-optical++ sensor to solve concrete functional problems was elaborated.
Assuntos
Circulação Sanguínea/fisiologia , Luz , Modelos Biológicos , Pletismografia de Impedância/métodos , Tecnologia de Fibra Óptica/instrumentação , Tecnologia de Fibra Óptica/métodos , Humanos , Matemática , Pletismografia de Impedância/instrumentação , TransdutoresAssuntos
Metaloporfirinas , Sarcoma Experimental/diagnóstico , Espectrometria de Fluorescência/métodos , Animais , Estudos de Avaliação como Assunto , Lasers , Medições Luminescentes , Camundongos , Camundongos Endogâmicos C57BL , Transplante de Neoplasias , Sarcoma Experimental/análise , Espectrometria de Fluorescência/instrumentação , ItérbioRESUMO
Experiments on 20 rabbits were made to explore the fluorescent properties of a water-soluble drug pyrroloindolysin administered to the blood vessels. The fluorescent spectra were excited in the UV spectrum. The drug was rapidly accumulated by the heart, with the fluorescence being mainly seen in the structures of the interatrial septum. The data obtained point to the possibility of the use of the described method for fluorescent studies of the different parts and structures of the heart.
