Evaluation of Cytotoxic and Genotoxic Effects in Buccal Mucosal Cells in Non-Smokers and Users of Traditional Combustible Tobacco Products and Non-Combustible Alternatives.

AIMS/OBJECTIVES: The aim of this cross-sectional observational study was to investigate cytogenetic damage to the buccal mucosa in non-smokers and consumers of traditional combustible tobacco products and non-combustible alternatives. METHODS: A total of 160 participants were divided into four groups according to the type of product used, including non-smokers, users of conventional combustible tobacco (cigarettes), heated tobacco, and electronic, tobacco-free vapor products (e-cigarettes). Buccal mucosa samples were analyzed using the micronucleus cytome assay to assess cytotoxic and genotoxic damage. RESULTS: E-cigarette users showed significantly higher values for all tested parameters in the micronucleus test compared to non-smokers (p < 0.05). Similarly, users of tobacco heating products showed an increase in all parameters (p < 0.05), with the exception of the number of cells with micronuclei. Conventional cigarette smokers showed a notable increase in the number of binucleated cells and cells with karyorrhexis and karyolysis (p ≤ 0.05). When assessing the differences between users of traditional combustible tobacco products and non-combustible alternatives, these did not appear to be significant, except for e-cigarette users, who had significantly more cells with condensed chromatin (p ≤ 0.001), while users of tobacco heating products had more pyknotic cells (p ≤ 0.001). CONCLUSION: The results of this study underscore the heightened occurrence of cytotoxic and genotoxic damage in users of both conventional combustible tobacco products and non-combustible alternatives compared to non-smokers, emphasizing the detrimental impact of these products on the oral mucosa.

Assessment of Cytotoxic and Genotoxic Effect of Modern Dental Materials in vivo.

Objectives: The aim of the study was to assess the biocompatibility of modern composite and amalgam dental fillings. Material and Methods: The research was conducted on 150 healthy patients between the ages of 10 and 20 who had amalgam and composite fillings between 6 and 12 months. Under in vivo conditions, a swab of buccal cells near the fillings was taken, and the cytotoxic and genotoxic impact of composite and amalgam fillings on these cells was analyzed using the extended micronucleus test (cytomeassay). Results: The results showed statistically significant differences between the groups of subjects with amalgam and composite fillings and subjects without fillings for the following parameters: number of micronuclei (p=0.006), number of buds (p<0.001), number of binuclear cells (p<0.001), number of nucleoplasmic bridges (p<0.001).The number of micronuclei was statistically significantly higher in the group of subjects with amalgam and composite fillings compared to the group without fillings. The results for nuclear buds, for the number of binuclear cells and the number of nucleoplasmic bridges showed that the group with amalgam fillings had a statistically significantly higher number of these changes compared to other groups.The results of the analysis of the relationship between the parameters of the micronucleus test and the number of amalgam and composite surfaces did not show statistically significant values. Parameters indicating cell cytotoxicity were not statistically significantly elevated in subjects with fillings. The results of the analysis of the influence of the patients' lifestyle on the results of the micronucleus test showed statistically significant results for certain predictors (diagnostic X-ray radiation, coffee consumption, consumption of cooked, dried meat and baked food). Conclusion: Based on the results, it can be concluded that the buccal cells of subjects with amalgam fillings showed the highest degree of genotoxic changes, followed by those with composite fillings and the least buccal cells of patients without fillings.

Association of Gut Lachnospiraceae and Chronic Spontaneous Urticaria.

(1) Background: Chronic spontaneous urticaria (CSU) has been linked to the dysbiosis of the gut microbiota. Furthermore, various studies have highlighted the anti-inflammatory properties of short-chain fatty acids (SCFAs), whose production is primarily regulated by the gut microbiota. However, only a few studies have investigated the role of major SCFA producers, such as Lachnospiraceae, in skin inflammatory diseases. (2) Goal: This study aimed to compare the abundance of Lachnospiraceae between CSU patients and healthy controls (HCs). (3) Material and methods: In this case-control study, 16S rRNA sequencing was performed to compare the composition of the gut microbiome between 22 CSU patients and 23 HCs. (4) Results: Beta-diversity revealed significant clustering (p < 0.05) between the CSU patients and HCs. Alpha diversity in the CSU group was significantly decreased according to the Evenness index (p < 0.05). The linear discriminant analysis effect size (LEfSe) identified the significant depletion of the Lachnospiraceae family in CSU patients. (5) Conclusion: Our study revealed the dysbiosis of the gut microbiota in CSU patients, including decreased levels of Lachnospiraceae members, responsible for SCFA production, suggesting that SCFAs may contribute to immune dysfunction in the pathogenesis of CSU. We speculate that the modulation of SCFAs could serve as a prospective additional option in CSU treatment.

Efficiency Evaluation of Various Solvents in Retreatment of Endodontic Filling in Extracted Teeth.

Objectives: The aim of this study was to evaluate the retreatment duration and efficacy of eucalyptol and tea tree oil in engine driven and manual retreatment of epoxy resin based endodontic filling in extracted human teeth. Methods and materials: Sixty human single rooted teeth were randomly divided into two groups. The first group was prepared using hand files, and the second one using Reciproc system. Root canals were filled with gutta-percha points and AH Plus sealer. Retreatment was performed using K-reamers and Hedström files for the first group, and Reciproc instruments for the second group. Each group was divided into two subgroups (n=15) according to the retreatment solvent used (eucalyptol or tea tree oil). Root canals were longitudinaly split and analized with stereomicroscope (15x magnification). The surface of the remaining filling material was measured using an image processing software. Results: There were no statistically significant differences in the area of residual filling material among used solvents, nor in applied technique. Retreatment with Reciproc instruments was significantly faster compared to retreatment using hand files. The manual retreatment technique was faster when tea tree essential oil was used as a solvent compared to eucalyptol. Conclusions: Australian tea tree oil and eucalyptus oil were equally effective in removing endodontic filling material, but none of canals were completely free of the residual filling material. Retreatment with Reciproc instruments was faster compared to retreatment using hand files. The manual retreatment technique is faster when tea tree essential oil is used as a solvent compared to eucalyptol.

Efficacy of Reciprocating Instruments in Retreatment of Bioactive and Resin-Based Root Canal Sealers.

Objective: To compare the effectiveness of reciprocating instruments in removing gutta-percha and bioactive-based (BioRoot RCS and MTA Fillapex) and epoxy resin-based (AH Plus) sealers from root canals based on filling residues and the time required for root canal revision. Material and methods: Root canals of 90 teeth were instrumented with Reciproc R40. All root canals were obturated using the single-cone technique with Reciproc R40 gutta-percha and with one of the selected sealers. Samples with oval, straight canals were used and randomly divided into three groups: (i) filled with AH Plus sealer and gutta-percha (n=30); (ii) filled with MTA Fillapex and gutta-percha (n=30); (iii) filled with BioRoot RCS and gutta-percha (n=30). Each group was divided into two subgroups (n=15) according to the retreatment instrument used (Reciproc M-Wire R25/R40 or Reciproc blue RB25/RB40). Root canals were longitudinally split and analyzed with a stereomicroscope at 15 × magnifications in the coronal, middle, and apical third. Computational analyses were performed with the Image J software. Data were compared using the Kruskal-Wallis test and Mann-Whitney U test. Results: While no statistically significant differences in the residual material surface were found for Reciproc Blue, Reciproc M-Wire showed significantly higher residual material surface for AH Plus and MTA Fillapex compared to BioRoot RCS. For AH plus. Residual material surface was significantly lower for Reciproc Blue than for Reciproc M-Wire. In contrast, BioRoot RCS showed a significantly higher residual material surface for Reciproc Blue. Conclusions: Calcium silicate-containing sealers were more retrievable compared to AH Plus, with fewer sealer remnants and shorter retreatment time. Retreatment with Reciproc M-Wire instruments was superior to Reciproc blue instruments in retreatment of BioRoot RCS. However, none of the sealers were removed completely.

Knowledge and Attitudes about Dental Trauma Among the Students of the University of Split.

OBJECTIVES: Dental trauma among children is a common problem, and everyone who works with them needs to possess appropriate knowledge and skills to provide proper care at the site of the accident. The aim was to evaluate the knowledge of emergency management of dental trauma among students who should be capable of managing such injuries in their future career. MATERIALS AND METHODS: A cross-sectional survey regarding dental trauma emergency management was conducted on 679 students from five different studies using a questionnaire. The obtained data were analyzed by the Student t-test or one - way ANOVAusing the Tukey's post-hoc test and multiple linear regression analysis (p<0.05). RESULTS: The total knowledge score regarding the emergency management of dental trauma among examined student was 4.32±2.25 (max 10). The positive association of the knowledge score was observed with the age of student (ß=0.722, p≤0.001). A similar relation was observed depending on whether they received dental injuries training during their academic education (ß=2.365, p≤0.001), as well as students' assessment of the importance of knowledge regarding dental trauma emergency management (ß=0.433, p≤0.001). CONCLUSIONS: It can be concluded that the surveyed students have limited knowledge regarding dental injuries and their emergency management procedures. The obtained data emphasized the importance of additional education of all professions that may encounter dental trauma injuries to improve the outcomes of dental trauma treatment.

In vivo evaluation of fluoride and sodium lauryl sulphate in toothpaste on buccal epithelial cells toxicity.

OBJECTIVES: The present study addresses the effect of fluoride and sodium lauryl sulphate content of toothpaste on oral epithelial cells in vivo conditions. SUBJECTS AND METHOD: Forty volunteers were assigned into two experimental groups, each of them applying the different brand of toothpaste. Every group has been using three different types of toothpaste (non-fluoride and non-SLS, fluoride and non-SLS, and the fluoride and SLS) of the same brand for 6 months, each for 2 months. The buccal epithelial cells were sampled at baseline and 30, 60, 90, 120, 150 and 180 days after the beginning of the research. Effect on DNA damage was analyzed by micronucleus assay Results: After 60 days of use, for both tested kinds of toothpaste with fluoride and without SLS, all studied parameters were not significantly different from the results obtained at the time when the participants used a non-fluoride toothpaste. While, after 60 days of use, for one kind of toothpaste with SLS and fluoride, was observed significantly higher incidence of pyknotic cells (2.20 ± 0.95, 0.00 ± 0.00 vs. 0.05 ± 0.22, respectively; p = .001), cells with karyorrhexis (2.35 ± 1.14, 0.85 ± 0.93 vs. 0.40 ± 0.68, respectively; p = .001), and nuclear buds (1.35 ± 0.68, 0.45 ± 0.51 vs. 0.45 ± 0.60, respectively; p = .001), compared to toothpastes of the same brand with fluoride and without SLS, and without fluoride and without SLS, for the same period. CONCLUSIONS: Based on the results, can be concluded that there is no fluorine-dependent cytotoxic or genotoxic effect, while SLS dentifrice increases the number of nuclear morphological changes in buccal epithelial cells.

In vivo assessment of genotoxicity in buccal cells of children undergoing tooth restoration.

OBJECTIVES: Whereas dental materials came in direct or close contact with oral tissue, it is a great concern about the biocompatibility of those materials. This study was performed to evaluate possible DNA damage to buccal cells exposed to dental materials. METHODS: This prospective, longitudinal clinical study was conducted over a three months period. Class II restorations were placed in 60 young patients with no previous filling using one of three tested dental materials (two glass ionomers; Ketac Molar and Ionofil Molar and one compomer material; Twinky Star). DNA damage was analysed by micronucleus assays, in buccal exfoliated epithelial cells. RESULTS: In patients treated with Ketac Molar, a significant frequency of micronuclei (p = 0.027) and binucleated cells in samples taken 30 days following restoration (p = 0.029) was confirmed. In patients treated with Twinky Star, a statistically significant increase in the number of binucleated cells in samples taken after 7 and 30 days following restoration (p = 0.001 and p < 0.001, respectively) was observed. In all samples collected 90 days after treatment, there was no statistical difference in the number of any cell changes. CONCLUSION: In this study long-term biocompatibility of tested materials was confirmed. Glass ionomers and compomers are widely used materials in paediatric dentistry, and this study has proved their safety for usage in children.

Assessment of cytotoxic and genotoxic effects of conventional and whitening kinds of toothpaste on oral mucosa cells.

OBJECTIVE: This study aimed to evaluate possible DNA damages to oral epithelial cells exposed to whitening kinds of toothpaste considering the effect of conventional non-whitening toothpaste. MATERIALS AND METHODS: Sixty volunteers were assigned into three experimental groups, each of them using a different regular toothpaste for the initial 2 months, followed by the use of whitening kind of toothpaste of the same brand for next 2 months. The oral epithelial cells were sampled prior and 30, 60, 90 and 120 days after the beginning of the use of tested kinds of toothpaste. Chromosomal damages were analyzed by micronucleus assay. RESULTS: For just one kind of tested whitening toothpaste was observed the significant increase in the number of micronucleated cells after 60 days of use compared values obtained 60 days of usage of conventional non-whitening toothpaste (6.35 ± 3.67 and 2.8 ± 1.91; p < .05). There was no statistically significant difference in other micronucleus assay endpoints between tested types of toothpaste at either of the sampling times during the period of toothpaste application. CONCLUSIONS: Based on the results, it can be concluded that the use of certain whitening kinds of toothpaste may cause a limited biologically insignificant genotoxic effect on buccal epithelial cells.

Cytogenetic damage in exfoliated oral buccal cells by dental composites.

PURPOSE: To evaluate the possible geno/cytotoxic effects of dental composite materials by assessing the frequency of micronuclei formation and other nuclear abnormalities in the exfoliated buccal epithelium. METHODS: Swabs were taken from the buccal mucosa of 85 young healthy subjects. All participants had healthy dentition or dentition restored only with composite materials. Genotoxicity and cytotoxicity was assessed by micronucleus assay. RESULTS: The results indicated no significant difference in number of oral mucosa cells with micronuclei in subjects with different numbers of composite restored tooth surfaces (P= 0.476). Also, the number of restored surfaces had no effect on nuclear alterations closely related to cytotoxicity, such as karyolysis (P= 0.572), karyorrehexis (P= 0.573) and picnosis (P= 0.765). CLINICAL SIGNIFICANCE: Despite doubts about the safe clinical use of resin composites, this study found no evidence that composite materials trigger long-term cytogenetic damage in the epithelial cells of buccal mucosa in humans. There is no objective and quantifiable evidence of genotoxicity induced by composite restorative materials in clinical practice.

Attitudes of Students of Differenet Schools of University of Zagreb on Tooth Bleaching.

OBJECTIVE: To compare the awareness that students from four different faculties within the University of Zagreb have of oral health and tooth bleaching procedure. MATERIALS AND METHODS: The study included 158 subjects (both male and female) - 38 students from the School of Dental Medicine and 40 students from each of the following faculties: the School of Medicine, the Faculty of Economics and the Faculty of Civil Engineering. The respondents were asked to fill out the survey with multiple choices by marking the answers they considered correct. RESULTS: Only 12% of the respondents followed the information on oral health. More than two thirds of all subjects brush their teeth twice a day, but there were no statistically significant differences between the subjects with respect to college or gender. More than half of the participants (55%) were satisfied, and 12% were completely satisfied with their dental appearance. About 80% of the respondents were aware of differences between teeth bleaching and teeth polishing procedures, with greater prevalence among Dental Medicine and Medicine students. 80% of all subjects would go to a dental office if they decided to whiten their teeth while less than a half (46%) of all the subjects believed that a tooth bleaching has some adverse side-effects. CONCLUSIONS: There is a difference in knowledge on oral hygiene and tooth bleaching between the students from the School of Dental Medicine, the School of Medicine, the Faculty of Economics and those from the Faculty of Civil Engineering. Dental students have the best knowledge on tooth bleaching and oral health, which was in accordance with their educational guidance and level of education.

In vitro detection of DNA damage in human leukocytes induced by combined effect of resin composites and adhesive systems.

PURPOSE: To simultaneously evaluate the genotoxicity of dental composites and adhesive systems in vitro using a cytogenetic assay, with respect to the influence of composite shade. METHODS: Genotoxicity assessment was carried out in human peripheral blood leukocytes using the comet assay. Three resin composite materials, two microhybrids and one nano-hybrid, in shade A1 and A3.5 were used with manufacturer-recommended four adhesive systems. Cultures were treated for 48 hours with samples after elusion for 1 hour, 1 day, 7 days or 30 days, in two different concentrations (4.16 mg/mL, 8.33 mg/mL). Kruskall-Wallis test was used for the statistical analysis (alpha = 0.05). RESULTS: For combinations of micro-hybrid composite (A3.5) with two self-etch adhesives (16.1 +/- 5.50 and 16.2 +/- 9.52) after exposure to samples eluted for 1 day, the incidence of primary DNA damage was significantly higher than for the corresponding negative control (14.7 +/- 2.85). Genotoxicity was also higher after treatment with samples eluted for 1 hour (15.3 +/- 4.70) and 1 day (15.3 +/- 9.10), comprised of nano-hybrid composite (A1) with self-etch adhesive in relation to the control (13.1 +/- 1.70). There was no clear trend of increased DNA damage in material combinations with darker shades of composites. Material composition and higher material concentrations showed greater influence on the genotoxicity.

Micronucleus, alkaline, and human 8-oxoguanine glycosylase 1 modified comet assays evaluation of glass-ionomer cements - in vitro.

The purpose of this study was to evaluate the genotoxic potential of components leached from two conventional self-curing glass-ionomer cements (Fuji IX and Ketac Molar), and light-curing, resin modified glass-ionomer cements (Vitrebond, Fuji II LC). Evaluation was performed on human lymphocytes using alkaline and hOGG1 modified comet, and micronucleus assays. Each material, polymerised and unpolymerised, was eluted in extracellular saline (1 cm2 mL-1) for 1 h, 1 day, and 5 days. Cultures were treated with eluates using final dilutions of 10(-2), 10(-3), and 10(-4). Alkaline comet assay did not detect changes in DNA migration of treated cells regardless of the ionomer tested, polymerisation state, and elution duration. Glass ionomers failed to significantly influence micronucleus frequency. No oxidative DNA damage in treated lymphocytes was observed using hOGG1 modified comet assay. Obtained results indicate high biocompatibility of all tested materials used in the study under experimental conditions.

Genotoxicity in gingival cells of patients undergoing tooth restoration with two different dental composite materials.

OBJECTIVES: Dental composite materials come into direct contact with oral tissue, especially gingival cells. This study was performed to evaluate possible DNA damage to gingival cells exposed to resin composite dental materials. MATERIALS AND METHODS: Class V restorations were placed in 30 adult patients using two different composite resins. The epithelial cells of the gingival area along the composite restoration were sampled prior to and after 7, 30, and 180 days following the restoration of the tooth. DNA damage was analysed by comet and micronucleus assays in gingival exfoliated epithelial cells. RESULTS: The results showed significantly higher comet assay parameters (tail length and % DNA in the tail) within periods of 30 and 180 days. The micronucleus test for the same exposure time demonstrated a higher number of cells with micronuclei, karyolysis, and nuclear buds. Results did not reveal any difference between the two composite materials for the same duration of exposure. CONCLUSION: Based on the results, we can conclude that the use of composite resins causes cellular damage. As dental composite resins remain in intimate contact with oral tissue over a long period of time, further research on their possible genotoxicity is advisable. CLINICAL RELEVANCE: Long-term exposure of gingival cells to two different composite materials demonstrated certain DNA damage. However, considering the significant decline in micronuclei frequency after 180 days and efficiency in the repair of primary DNA damage, the observed effects could not be indicated as biologically relevant.

Composite-induced toxicity in human gingival and pulp fibroblast cells.

OBJECTIVE: The most important requirement for a material to be used in medical applications is its biocompatibility. Dental composite materials come into direct contact with oral tissues, especially gingival and pulpal cells. This study was performed to evaluate possible DNA damage in cells of human origin exposed to dental composites in vitro using a cytogenetic assay. MATERIALS AND METHODS: Two composite resins (Vertise Flow, Kalore) were tested on human gingival and pulp fibroblasts using the acridine orange/ethidium bromide viability staining and alkaline comet assay. Cultures were treated with polymerized composites in two different concentrations (20 mg/ml, 40 mg/ml) for 14 days. Chi-square and Kruskall-Wallis non-parametric test were used for the statistical analysis (p < 0.05). RESULTS: Significant cytotoxicity was observed for 40 mg/ml of Vertise Flow in both cultures, while Kalore (40 mg/ml) showed cytotoxic effect only on human pulp fibroblasts. A significant level of DNA damage was detected for both materials and concentrations, in both cell cultures. CONCLUSION: If the two cell cultures are compared, the pulp cells were more sensitive to the cyto/genotoxic effects of dental composites. Based on the results, one can conclude that the use of tested materials may cause cellular damage in gingival and pulp fibroblasts in vitro.

Attitude towards Oral Health at Various Colleges of the University of Zagreb: A Pilot Study.

PURPOSE: The aim of this study was to compare the oral status of three various groups of students: students of the School of Dental Medicine, students of technical sciences and students of humanities. MATERIAL AND METHODS: Research included 58 students of the University of Zagreb. They answered 3 questions: how often they brush their teeth, how often they visit their dentist and how important dental health is to them. After a standard dental check-up we calculated the DMFT index. They were given an indicator for plaque Mira-2-Ton® (Hager Werken, Duisburg, Germany) and we calculated the plaque index. For statistical analysis the ANOVA test was used. RESULTS: Students of the School of Dental Medicine had a lower plaque index than other students and this was statistically significant (p=0.0018; f=7.14). They also had a lower DMFT index, but it was not statistically significant (p=0.1004; f=2.4). 83% of students said that they brushed their teeth 2-3 times a day. Only 17% of all students brush their teeth more than 3 times a day and they are all students of the School of Dental Medicine (21% of them). Perception of oral health is on a high level, but perception of oral disease is not. The social approval of the answer was also an important factor. CONCLUSION: Students of the School of Dental Medicine are an illustrative example of improving our habits due to education.

An in vitro morphological investigation of the endodontic spaces of third molars.

Aim of this paper was to investigate the particular anatomic features of the endodontic space of third molars in the general population of Croatia. A total of 106 fully developed third molars (56 maxillar and 50 mandibular) has been analyzed. The respective number of roots and of root canals, the structure of the roots, and the curvature and absolute length of the root-canals were analyzed. In most cases, upper third molars had three roots (83.9%), while most of the lower molars were single rooted (56.0%). Upper third molars had mostly three root canals (75.1%), lower third molars two (90.0%). In both jaws, most third molars had curved canals (60.7% in the upper and 84.0% in the lower jaw). 12.5% of upper third molars had lateral and accessory canals, whereas only 4.0% of the lower third molars were found to have them. The Student t-test showed statistically significant differences (p < 0.05) in the length of the root-canals, mesiobuccal and distobuccal canals being significantly shorter than palatinal canals. The same test showed that in the lower third molars the mesial root canals were significantly longer that the distal ones, although slightly. The results obtained are in compliance with similar results by other authors; however, they tend to show some specific population-related features.

Genotoxic biomonitoring of flowable and non-flowable composite resins in peripheral blood leukocytes.

OBJECTIVE: Composite restorative materials represent one of the most important groups of materials in contemporary dental practice. However, their incomplete polymerization may lead to monomer-induced genotoxicity. The objective of this study was to evaluate the genotoxicity of three flowable (Filtek Supreme XT Flow, Tetric EvoFlow, Gradia Direct Flo) and three non-flowable dental composite materials (Filtek Z250, Tetric EvoCeram, Gradia Direct Posterior). MATERIALS AND METHODS: Genotoxicity assessment of composite materials was carried out in vitro in human peripheral blood leukocytes using the alkaline single-cell gel electrophoresis technique (comet assay). Prepared materials were eluted in saline solution for 1 h, 1 day and 5 days. Thereafter leukocyte cultures were treated with different concentrations of eluates obtained from each of the tested dental composite materials. Kruskall-Wallis non-parametric test was used for statistical analysis (p < 0.05). RESULTS: The tested materials did not show genotoxic effects after exposure of leucocytes to 1 h eluates. Culture treated with 1 day eluates of all tested materials, only at a highest concentration (10(-2)), affected the measured cytogenetic parameters. Of all tested materials, only Filtek Z250 and Filtek Supreme XT Flow did not exhibit a genotoxic effect in cultures that were under the influence of 5 day eluates. CONCLUSION: Tested materials exhibited limited genotoxic activity in peripheral blood leukocytes. Since the effect was observed only in leukocyte cultures treated by 1-day eluates at the highest concentration (10(-2)) and it decreases in cultures exposed to 5 day eluates, it should not pose a significant risk to the human genome.

Effects of dental adhesives on micronucleus frequency in peripheral blood lymphocytes in vitro.

Dental adhesives come into direct contact with oral tissues. Due to this close and long-term contact, the materials should exhibit a high degree of biocompatibility. The aim of this study was to evaluate the genotoxic effect of dental adhesives on human lymphocytes in vitro. Polymerized dental adhesives (Excite, Adper Single Bond 2, Prompt L-pop and OptiBond Solo Plus) were eluted in dimethyl sulfoxide for 1 hour, 24 h and 120 h (5 days). Thereafter, lymphocyte cultures were treated with different concentrations of eluates (0.2 microg/mL, 0.5 microg/mL and 5 microg/mL) obtained from each of the tested materials. Genotoxicity was evaluated by micronucleus test. The chi2-test was used on statistical analysis (p < 0.05). After elution period of 1 h, only the highest dose of all tested materials affected the measured cytogenetic parameters. After 24 h, genotoxicity was demonstrated only in cultures treated with eluates in concentrations of 0.5 microg/mL and 5 microg/mL. Based on the results, it is concluded that the use of dental adhesives causes genotoxic effects in human lymphocytes. Toxic effect of these dental adhesives increases with the tested material concentration and decreases with the length of elution period.