Flower color mutation, pink to orange, through CmGATA4 - CCD4a-5 module regulates carotenoids degradation in chrysanthemum.

The carotenoids biosynthesis pathway in plants has been studied extensively, yet little is known about the regulatory mechanisms underlying this process, especially for ornamental horticulture plants. In this study, a natural variation of chrysanthemum with orange coloration was identified and compared with the wild type with pink coloration; the content and component of carotenoids were largely enriched in the mutant with orange coloration. CmCCD4a-5, the DNA sequence in both 'Pink yan' and the mutant, was identified and shown to function as a carotenoid degradation enzyme. Compared with 'Pink yan', the mutant shows lower expression level of CmCCD4a-5. Furthermore, CmGATA4 was found to have an opposite expression trend to CmCCD4a-5, and it could directly bind with the CmCCD4a-5 promoter. Taken together, this study demonstrates that CmGATA4 acts as a negative regulator of CmCCD4a-5 and, furthermore, low expression of CmCCD4a-5 resulted in carotenoid accumulation in the mutant.

Chilling and gibberellin acids hyperinduce ß-1,3-glucanases to reopen transport corridor and break endodormancy in tree peony (Paeonia suffruticosa).

Bud endodormancy is accompanied by transport channel apertures blockage through callose deposition, and its resume to growth requires evoking ß-1,3-glucanases (BGs) to unchoke the conduit. To understand out its working manner, the statuses of the transport channels were evaluated and candidate BGs were identified during chilling and gibberellin acids (GA) induced dormancy release in tree peony. Calcein reflects plasmodesmata permeability, and no calcein was observed in the bud together with density aniline blue fluorescent around the stem phloem at 0 d chilling. With the increase of chilling accumulation, the contents of glucan declined and the activities of gulcanase increased gradually in buds, and the calcein reached the top of flower primordia at 21 d chilled bud. Both GA3 and GA4 feedings promoted bud sprouting and growth along with rapidly unchoking the transport channels, and GA3 was more effective. Several candidate ß-1,3-glucanase genes were detected, combining transcriptional profiling and quantitative PCR analysis. PsBG1, PsBG3, PsBG6, PsBG8 and PsBG9 were inducible by chilling accumulation and presented laminarin hydrolyzing activities after prokaryotically expression, while PsBG1, PsBG3, PsBG8 and PsBG9 responded to GAs application. Subcellular localizations revealed that PsBG6 and PsBG9 were plasmodesmata residents. It was concluded that PsBG6 played a vital role in chilling accumulation response and PsBG9 was central in GAs-induced dormancy release, and they could be used as marker genes for dormancy release in tree peony. These results were of great value to understand the mechanism of dormancy regulation and as an important fundamental for forcing culture technology in tree peony.

Simultaneous measurement of refractive index, strain, and temperature based on a Mach-Zehnder interferometer with hybrid structure optical fiber.

An optical fiber sensor based on a Mach-Zehnder interferometer with hybrid structure optical fiber for simultaneous measurement of refractive index (RI), strain, and temperature is proposed and demonstrated. The proposed structure is a hybrid structure based on a non-core fiber combined with few-mode fiber. The possibility of simultaneously measuring RI, strain, and temperature relies on the different sensitivity responses of three resonance peaks in the transmission spectrum. Thus, simultaneous measurement of RI, strain, and temperature is realized by calculating the wavelength shift of the three resonance peaks. The experimental results show that the sensitivities of RI are 22.9 pm/RIU, 24.6 pm/RIU, and 97 pm/RIU when RI changes from 1.3707 to 1.39809, respectively. The sensitivities of strain are $ - {3.5}\,\, \text{pm}/ \unicode{x00B5}\unicode{x03B5}$-3.5pm/µÎµ, $ - {1.9}\,\, \text{pm}/ \unicode{x00B5} \unicode{x03B5}$-1.9pm/µÎµ, and $ - {4.1}\,\, \text{pm}/ \unicode{x00B5} \unicode{x03B5}$-4.1pm/µÎµ in the range from 0 to 1400 pm/µ$\unicode{x03B5}$ε. The sensitivities of temperature ranging from 35°C to 55°C are 162 pm/°C, 194 pm/°C, and 162 pm/°C, respectively. The proposed sensor, with advantages of simple configuration, compact structure, and high sensitivity, exhibits great potential in fields of multi-parameter measurement.

Identification and characterization of microRNAs in tree peony during chilling induced dormancy release by high-throughput sequencing.

Tree peony, one of the most valuable horticultural and medicinal plants in the world, has to go through winter to break dormancy. Growing studies from molecular aspects on dormancy release process have been reported, but inadequate study has been done on miRNA-guided regulation in tree peony. In this study, high-throughput sequencing was employed to identify and characterize miRNAs in three libraries (6 d, 18 d and 24 d chilling treatments). There were 7,122, 10,076 and 9,097 unique miRNA sequences belonging to 52, 87 and 68 miRNA families, respectively. A total of 32 conserved miRNAs and 17 putative novel miRNAs were identified during dormancy release. There were 771 unigenes as potential targets of 62 miRNA families. Total 112 known miRNAs were differentially expressed, of which 55 miRNAs were shared among three libraries and 28 miRNAs were only found in 18 d chilling duration library. The expression patterns of 15 conserved miRNAs were validated and classified into four types by RT-qPCR. Combining with our microarray data under same treatments, five miRNAs (miR156k, miR159a, miR167a, miR169a and miR172a) were inversely correlated to those of their target genes. Our results would provide new molecular basis about dormancy release in tree peony.