Oncogene-addicted solid tumors and microbiome-lung cancer as a main character: a narrative review.

Background and Objective: Lung cancer stands as the main cause of cancer-related deaths worldwide. With the advent of immunotherapy and the discovery of targetable oncogenic driver genes, although prognosis has changed in the last few years, survival rates remain dismal for most patients. This emphasizes the urgent need for new strategies that could enhance treatment in precision medicine. The role of the microbiota in carcinogenesis constitutes an evolving landscape of which little is known. It has been suggested these microorganisms may influence in responses, resistance, and adverse effects to cancer treatments, particularly to immune checkpoint blockers. However, evidence on the impact of microbiota composition in oncogene-addicted tumors is lacking. This review aims to provide an overview of the relationship between microbiota, daily habits, the immune system, and oncogene-addicted tumors, focusing on lung cancer. Methods: A PubMed and Google Scholar search from 2013 to 2024 was conducted. Relevant articles were reviewed in order to guide our research and generate hypothesis of clinical applicability. Key Content and Findings: Microbiota is recognized to participate in immune reprogramming, fostering inflammatory, immunosuppressive, or anti-tumor responses. Therefore, identifying the microbiota that impact response to treatment and modulating its composition by interventions such as dietary modifications, probiotics or antibiotics, could potentially yield better outcomes for cancer patients. Additionally, targeted therapies that modulate molecular signaling pathways may impact both immunity and microbiota. Understanding this intricate interplay could unveil new therapeutic strategies. Conclusions: By comprehending how microbiota may influence efficacy of targeted therapies, even though current evidence is scarce, we may generate interesting hypotheses that could improve clinical practice.

Trends in hospital capacity and utilization in Puerto Rico by health regions, 2010-2020.

Like many under resourced, island communities, most of the municipalities in Puerto Rico are medically underserved. However, there is limited information about changes in hospital capacity and any regional disparities in availability of hospital services in Puerto Rico, especially given the multiple public health emergencies the island has faced in recent years (e.g. hurricanes, earthquakes, and COVID-19). This study described the trends in hospital capacity and utilization for the Island of Puerto Rico and by health regions from 2010 to 2020. We analyzed the 2021-22 Area Health Resource File (AHRF) and aggregated the data by seven health regions, which are groupings of municipalities defined by the Puerto Rico Department of Health. Ten-year estimates for hospital utilization were adjusted for population size by health region. During the more recent five-year period, there were decreases in hospitals, hospital beds, and surgeries, which represent a shift from the earlier five-year period. Over the 10 years of the study period, there was an overall decrease in population-adjusted measures of hospital utilization on the island of Puerto Rico-despite multiple disasters that would, theoretically, increase need for health care services. We also found variation in hospital capacity and utilization by health regions indicating the rate of change was not uniform across Puerto Rico. The capacity of Puerto Rico's hospital system has shrunk over the past decade which may pose a challenge when responding to recurrent major public health emergencies, especially within specific health regions.

United States Federal Policies Contributing to Health and Health Care Inequities in Puerto Rico.

Puerto Rico, a territory of the United States since 1898, has recently experienced an increasing frequency and intensity of natural disasters and public health emergencies. In 2022, Hurricane Fiona became the latest storm to attract media attention and cast a light on Puerto Rico's deteriorating conditions, including infrastructural failings, health care provider shortages, and high levels of chronic illness. Although recent events have been uniquely devastating, decades of inequitable US federal policy practices have fueled the persistence of health inequities in the territory. Here we demonstrate how existing health and health care inequities in Puerto Rico have been exacerbated by compounding disasters but are rooted in the differential treatment of the territory under US federal policies. Specifically, we focus on the unequal US Federal Emergency Management Agency response to disasters in the territory, the lack of parity in federal Medicaid funding for Puerto Rico, and Puerto Rico's limited political power as a territory of the United States. We also provide empirically supported policy recommendations aimed at reducing health and health care inequities in the often-forgotten US territory of Puerto Rico. (Am J Public Health. 2024;114(S6):S478-S484. https://doi.org/10.2105/AJPH.2024.307585) [Formula: see text].

Diabetes current and future translatable therapies.

Diabetes is one of the major diseases and concerns of public health systems that affects over 200 million patients worldwide. It is estimated that 90% of these patients suffer from diabetes type 2, while 10% present diabetes type 1. This type of diabetes and certain types of diabetes type 2, are characterized by dysregulation of blood glycemic levels due to the total or partial depletion of insulin-secreting pancreatic ß-cells. Different approaches have been proposed for long-term treatment of insulin-dependent patients; amongst them, cell-based approaches have been the subject of basic and clinical research since they allow blood glucose level sensing and in situ insulin secretion. The current gold standard for insulin-dependent patients is on-demand exogenous insulin application; cell-based therapies aim to remove this burden from the patient and caregivers. In recent years, protocols to isolate and implant pancreatic islets from diseased donors have been developed and tested in clinical trials. Nevertheless, the shortage of donors, along with the need of immunosuppressive companion therapies, have pushed researchers to focus their attention and efforts to overcome these disadvantages and develop alternative strategies. This review discusses current tested clinical approaches and future potential alternatives for diabetes type 1, and some diabetes type 2, insulin-dependent patients. Additionally, advantages and disadvantages of these discussed methods.

Prevalence of Chronic Pain by Immigration Status and Latino Ethnicity.

OBJECTIVE: The goal of this study was to evaluate the Latino paradox and healthy migrant hypotheses by estimating the association between the prevalence of chronic pain, immigration status, and Latino ethnicity. METHODS: This cross-sectional study analyzed pooled data from 85,395 adult participants of the 2019-2021 National Health Interview Survey. The dependent variables were any pain and chronic pain in the past 3 months. The independent variables were immigration status (US born, naturalized citizen, non-citizen) and Latino ethnicity. RESULTS: Chronic pain was prevalent for nearly a quarter of US born non-Latino adults (24%) and non-citizen non-Latino adults had the lowest prevalence at 8%. In multivariable adjusted models, US born non-Latino immigration status and ethnicity was associated with a higher probability of reporting chronic pain in the last 3 months compared to US born Latino adults (-3.0%; 95% CI = -4.4%, -1.6%), naturalized citizen non-Latino adults (-4.7%; 95% CI = -5.9%, -3.4%), naturalized citizen Latino adults (-6.7%; 95% CI = -8.5%, -4.9%), non-citizen non-Latino adults (-3.1%; 95% CI = -4.7%, -1.5%), and non-citizen Latino adults (-8.9%; 95% CI = -10.8%, -7.0%). CONCLUSION: US Born non-Latino adults reported the highest prevalence of chronic pain and non-citizen Latino adults reported the lowest prevalence of chronic pain providing support for the Latino paradox and healthy migrant effect hypotheses.

Health Care Access and Utilization and the Latino Health Paradox.

BACKGROUND: The Latino health paradox is the phenomenon whereby recent Latino immigrants have, on average, better health outcomes on some indicators than Latino immigrants who have lived in the United States longer and US-born Latinos and non-Latino Whites. This study examined whether the paradox holds after accounting for health care access and utilization. METHODS: The 2019-2020 National Health Interview Survey data were used. The main predictors included population groups of foreign-born and US-born Latinos (Mexican or non-Mexican) versus US-born non-Latino Whites. Predicted probabilities of health outcomes (self-reported poor/fair health, overweight/obesity, hypertension, coronary heart disease, diabetes, cancer, and depression) were calculated and stratified by length of residence in the United States (<15 or ≥15 years) among foreign-born Latinos and sex (female or male). Multivariable analyses adjusted for having a usual source of care other than the emergency department, health insurance, a doctor visit in the past 12 months, predisposing and enabling factors, and survey year. RESULTS: After adjusting for health care access, utilization, and predisposing and enabling factors, foreign-born Latinos, including those living in the United States ≥15 years, had lower predicted probabilities for most health outcomes than US-born non-Latino Whites, except overweight/obesity and diabetes. US-born Latinos had higher predicted probabilities of overweight/obesity and diabetes and a lower predicted probability of depression than US-born non-Latino Whites. CONCLUSIONS: In this national survey, the Latino health paradox was observed after adjusting for health care access and utilization and predisposing and enabling factors, suggesting that, although these are important factors for good health, they do not necessarily explain the paradox.

Black carbon in urban soils: land use and climate drive variation at the surface.

BACKGROUND: Black carbon (BC) encompasses a range of carbonaceous materials--including soot, char, and charcoal--derived from the incomplete combustion of fossil fuels and biomass. Urban soils can become enriched in BC due to proximity to these combustion sources. We conducted a literature review of BC in urban soils globally and found 26 studies reporting BC and total organic carbon (TOC) content collected to a maximum of 578 cm depth in urban soils across 35 cities and 10 countries. We recorded data on city, climate, and land use/land cover characteristics to examine drivers of BC content and contribution to TOC in soil. RESULTS: All studies were conducted in the northern hemisphere, with 68% of the data points collected in China and the United States. Surface samples (0-20 cm) accounted for 62% of samples in the dataset. Therefore, we focused our analysis on 0-10 cm and 10-20 cm depths. Urban soil BC content ranged from 0-124 mg/g (median = 3 mg/g) at 0-10 cm and from 0-53 mg/g (median = 2.8 mg/g) at 10-20 cm depth. The median proportional contribution of BC to TOC was 23% and 15% at 0-10 cm and 10-20 cm, respectively. Surface soils sampled in industrial land use and near roads had the highest BC contents and proportions, whereas samples from residential sites had among the lowest. Soil BC content decreased with mean annual soil temperature. CONCLUSIONS: Our review indicates that BC comprises a major fraction (nearly one quarter) of the TOC in urban surface soils, yet sampling bias towards the surface could hide the potential for BC storage at depth. Land use emerged as an importer driver of soil BC contents and proportions, whereas land cover effects remain uncertain. Warmer and wetter soils were found to have lower soil BC than cooler and drier soils, differences that likely reflect soil BC loss mechanisms. Additional research on urban soil BC at depth and from diverse climates is critical to better understand the role of cities in the global carbon cycle.

Satellite-based aerosol optical depth estimates over the continental U.S. during the 2020 wildfire season: Roles of smoke and land cover.

Wildfires produce smoke that can affect an area >1000 times the burn extent, with far-reaching human health, ecologic, and economic impacts. Accurately estimating aerosol load within smoke plumes is therefore crucial for understanding and mitigating these impacts. We evaluated the effectiveness of the latest Collection 6.1 MODIS Multi-Angle Implementation of Atmospheric Correction (MAIAC) algorithm in estimating aerosol optical depth (AOD) across the U.S. during the historic 2020 wildfire season. We compared satellite-based MAIAC AOD to ground-based AERONET AOD measurements during no-, light-, medium-, and heavy-smoke conditions identified using the Hazard Mapping System Fire and Smoke Product. This smoke product consists of maximum extent smoke polygons digitized by analysts using visible band imagery and classified according to smoke density. We also examined the strength of the correlations between satellite- and ground-based AOD for major land cover types under various smoke density levels. MAIAC performed well in estimating AOD during smoke-affected conditions. Correlations between MAIAC and AERONET AOD were strong for medium- (r = 0.91) and heavy-smoke (r = 0.90) density, and MAIAC estimates of AOD showed little bias relative to ground-based AERONET measurements (normalized mean bias = 3 % for medium, 5 % for heavy smoke). During two high AOD, heavy smoke episodes, MAIAC underestimated ground-based AERONET AOD under mixed aerosol (i.e., smoke and dust; median bias = -0.08) and overestimated AOD under smoke-dominated (median bias = 0.02) aerosol. MAIAC most overestimated ground-based AERONET AOD over barren land (mean NMB = 48 %). Our findings indicate that MODIS MAIAC can provide robust estimates of AOD as smoke density increases in coming years. Increased frequency of mixed aerosol and expansion of developed land could affect the performance of the MAIAC algorithm in the future, however, with implications for evaluating wildfire-associated health and welfare effects and air quality standards.

Soil Black Carbon Increases Under Urban Trees with Road Density and Time: Opportunity Hotspots for Carbon Storage in Urban Ecosystems.

Black carbon (BC) can comprise a significant fraction of the soil carbon pool in cities. However, vegetation cover and human activity influence the spatial distribution of urban soil BC. We quantified soil total carbon (TC), soil organic carbon (SOC), BC, and total nitrogen (TN) in a medium-sized city in Dallas-Fort Worth, Texas. Soils were sampled to 20 cm depth from underneath 16 paired Quercus stellata (post oak) trees and open lawns. Effects of vegetation cover, road density, and building age (a proxy for time since development) on soil C and N were analyzed. Soil OC concentrations were higher under post oak trees (5.5%) compared to open lawns (3.6%) at 0-10 cm, but not at 10-20 cm depth. In contrast, soil BC and TN did not differ by vegetation cover. There were significant interaction effects between vegetation cover and road density and vegetation cover and building age on soil BC. At 0-10 cm, soil BC concentrations, stock, and BC/SOC ratios increased more with road density under trees than lawns, indicating enhanced atmospheric BC deposition to tree canopies. Black carbon in tree soils also increased with building age as compared to lawn soils, likely due to higher BC retention under trees, enhanced BC losses under lawns, or both. Our findings show that urban tree soils are localized opportunity hotspots for BC storage in areas with elevated emissions and longer time since development. Conserving and planting urban trees above permeable surfaces and soils could contribute to long-term carbon storage in urban ecosystems.

Shower thoughts: why scientists should spend more time in the rain.

Stormwater is a vital resource and dynamic driver of terrestrial ecosystem processes. However, processes controlling interactions during and shortly after storms are often poorly seen and poorly sensed when direct observations are substituted with technological ones. We discuss how human observations complement technological ones and the benefits of scientists spending more time in the storm. Human observation can reveal ephemeral storm-related phenomena such as biogeochemical hot moments, organismal responses, and sedimentary processes that can then be explored in greater resolution using sensors and virtual experiments. Storm-related phenomena trigger lasting, oversized impacts on hydrologic and biogeochemical processes, organismal traits or functions, and ecosystem services at all scales. We provide examples of phenomena in forests, across disciplines and scales, that have been overlooked in past research to inspire mindful, holistic observation of ecosystems during storms. We conclude that technological observations alone are insufficient to trace the process complexity and unpredictability of fleeting biogeochemical or ecological events without the shower thoughts produced by scientists' human sensory and cognitive systems during storms.

Trends in Routine Checkup Within the Past Year Following a Hurricane.

The goal of this nationally representative, cross-sectional study is to evaluate the trends in routine checkup within the last year associated with exposure to a hurricane. We compared Puerto Rico (2017 Hurricane Irma, Hurricane Maria), Texas (2017 Hurricane Harvey), and Florida (2017 Hurricane Irma, Hurricane 2018 Michael) with states that had a category 1-2 hurricane make landfall from 2014 to 2019: Georgia, Louisiana, Mississippi, North Carolina, and South Carolina. We found that states impacted by a major hurricane in 2017 had a drop in routine checkup while the states that experienced a category 1-2 landfall did have a change in that year. By the following year, all states reported an increase in routine checkup suggesting that the disruption in routine care was temporary.

Undocumented Latino Immigrants and the Latino Health Paradox.

INTRODUCTION: Despite having worse healthcare access and other social disadvantages, immigrants have, on average, better health outcomes than U.S.-born individuals. For Latino immigrants, this is known as the Latino health paradox. It is unknown whether this phenomenon applies to undocumented immigrants. METHODS: This study used restricted California Health Interview Survey data from 2015 to 2020. Data were analyzed to test the relationships between citizenship/documentation status and physical and mental health among Latinos and U.S.-born Whites. Analyses were stratified by sex (male/female) and length of U.S. residence (<15 years/>= 15 years). RESULTS: Undocumented Latino immigrants had lower predicted probabilities of reporting any health condition, asthma, and serious psychological distress and had a higher probability of overweight/obesity than U.S.-born Whites. Despite having a higher probability of overweight/obesity, undocumented Latino immigrants did not have probabilities of reporting diabetes, high blood pressure, or heart disease different from those of U.S.-born Whites after adjusting for having a usual source of care. Undocumented Latina women had a lower predicted probability of reporting any health condition and a higher predicted probability of overweight/obesity than U.S.-born White women. Undocumented Latino men had a lower predicted probability of reporting serious psychological distress than U.S.-born White men. There were no differences in outcomes when comparing shorter- with longer-duration undocumented Latino immigrants. CONCLUSIONS: This study observed that the Latino health paradox may express patterns for undocumented Latino immigrants that are different from those for other Latino immigrant groups, emphasizing the importance of accounting for documentation status when conducting research on this population.

Growing inequities in mental health crisis services offered to indigent patients in Puerto Rico versus the US states before and after Hurricanes Maria and Irma.

OBJECTIVE: To assess changes in the availability of mental health crisis services in Puerto Rico relative to US states before and after Hurricanes Maria and Irma. DATA SOURCES/STUDY SETTING: National Mental Health Services Surveys conducted in 2016 and 2020. STUDY DESIGN: Repeated cross-sectional design. The independent variable was mental health facility location in Puerto Rico or a US state. Dependent variables were the availability of three mental health crisis services (psychiatric emergency walk-in services, suicide prevention services, and crisis intervention team services). DATA COLLECTION/EXTRACTION METHODS: The proportion and per 100,000 population rate of facilities offering crisis services were calculated. PRINCIPAL FINDINGS: The availability of crisis services at mental health facilities in Puerto Rico remained stable between 2016 and 2020. These services were offered less at indigent care facilities in Puerto Rico than US states (e.g., 38.2% vs. 49.5% for suicide prevention, p = 0.06) and the magnitude of difference increased following Hurricane Maria. CONCLUSIONS: There are disparities between Puerto Rico and US states in the availability of mental health crisis services for indigent patients.

Urban edge trees: Urban form and meteorology drive elemental carbon deposition to canopies and soils.

Urban tree canopies are a significant sink for atmospheric elemental carbon (EC)--an air pollutant that is a powerful climate-forcing agent and threat to human health. Understanding what controls EC deposition to urban trees is therefore important for evaluating the potential role of vegetation in air pollution mitigation strategies. We estimated wet, dry, and throughfall EC deposition for oak trees at 53 sites in Denton, TX. Spatial data and airborne discrete-return LiDAR were used to compute predictors of EC deposition, including urban form characteristics, and meteorologic and topographic factors. Dry and throughfall EC deposition varied 14-fold across this urban ecosystem and exhibited significant variability from spring to fall. Generalized additive modeling and multiple linear regression analyses showed that urban form strongly influenced tree-scale variability in dry EC deposition: traffic count as well as road length and building height within 100-150 m of trees were positively related to leaf-scale dry deposition. Rainfall amount and extreme wind-driven rain from the direction of major pollution sources were significant drivers of throughfall EC. Our findings indicate that complex configurations of roads, buildings, and vegetation produce "urban edge trees" that contribute to heterogeneous EC deposition patterns across urban systems, with implications for greenspace planning.

The impact of Medicaid funding structures on inequities in health care access for Latinos in New York, Florida, and Puerto Rico.

OBJECTIVE: To study the impact of Medicaid funding structures before and after the implementation of the Affordable Care Act (ACA) on health care access for Latinos in New York (Medicaid expansion), Florida (Medicaid non-expansion), and Puerto Rico (Medicaid block grant). DATA SOURCES: Pooled state-level data for New York, Florida, and Puerto Rico from the 2011-2019 Behavioral Risk Factor Surveillance System and data from the 2011-2019 American Community Survey and Puerto Rico Community Survey. STUDY DESIGN: Cross-sectional study using probit with predicted margins to separately compare four health care access measures among Latinos in New York, Florida, and Puerto Rico (having health insurance coverage, having a personal doctor, delayed care due to cost, and having a routine checkup). We also used difference-in-differences to measure the probability percent change of having any health insurance and any public health insurance before (2011-2013) and after (2014-2019) the ACA implementation among citizen Latinos in low-income households. DATA COLLECTION: The sample consisted of Latinos aged 18-64 residing in New York, Florida, and Puerto Rico from 2011 to 2019. PRINCIPAL FINDINGS: Latinos in Florida had the lowest probability of having health care access across all four measures and all time periods compared with those in New York and Puerto Rico. While Latinos in Puerto Rico had greater overall health care access compared with Latinos in both states, health care access in Puerto Rico did not change over time. Among citizen Latinos in low-income households, New York had the greatest post-ACA probability of having any health insurance and any public health insurance, with a growing disparity with Puerto Rico (9.7% any [1.6 SE], 5.2% public [1.8 SE]). CONCLUSIONS: Limited Medicaid eligibility (non-expansion of Florida's Medicaid program) and capped Medicaid funds (Puerto Rico's Medicaid block grant) contributed to reduced health care access over time, particularly for citizen Latinos in low-income households.

Wet nitrogen (N) deposition to urban Latin America: filling in the gaps with GEOS-Chem.

In Latin America, atmospheric deposition is a major vector of nitrogen (N) input to urban systems. Yet, measurements of N deposition are sparse, precluding analysis of spatial patterns, temporal trends, and ecosystem impacts. Chemical transport models can be used to fill these gaps in the absence of dense measurements. Here, we evaluate the performance of a global 3-D chemical transport model in simulating spatial and interannual variation in wet inorganic N (NH4-N + NO3-N) deposition across urban areas in Latin America. Monthly wet and dry inorganic N deposition to Latin America were simulated for the period 2006-2010 using the GEOS-Chem Chemical Transport Model. Published estimates of observed wet or bulk inorganic N deposition measured between 2006-2010 were compiled for 16 urban areas and then compared with model output from GEOS-Chem. Observed mean annual inorganic N deposition to the urban study sites ranged from 5.7-14.2 kg ha-1 yr-1, with NH4-N comprising 48-90% of the total. Results show that simulated N deposition was highly correlated with observed N deposition across sites (R2 = 0.83, NMB = -50%). However, GEOS-Chem generally underestimated N deposition to urban areas in Latin America compared to observations. Underestimation due to bulk sampler dry deposition artifacts was considered and improved bias without improving correlation. In contrast to spatial variation, the model did not capture year-to-year variation well. Discrepancies between modeled and observed values exist, in part, because of uncertainties in Latin American N emissions inventories. Our findings indicate that even at coarse spatial resolution, GEOS-Chem can be used to simulate N deposition to urban Latin America, improving understanding of regional deposition patterns and potential ecological effects.

Influence of sharpening filters on the detection of root fractures using low-dose cone-beam computed tomography.

OBJECTIVES: To evaluate the influence of sharpening filters in the detection of root fractures using low-dose cone-beam computed tomography (CBCT). MATERIALS AND METHODS: Eighty-four CBCT volumes acquired at three mA levels of 28 teeth inserted in the dental socket of dry human skull were selected from a previous study. The teeth were divided into four groups according to the presence and absence of root fracture and endodontic filling. Five radiologists evaluated all CBCT volumes for the presence of root fracture with and without the application of "Sharpen 1x" and "Sharpen 2x" filters in OnDemand3D software. Area under the ROC curve (AUC), sensitivity, specificity, and inter- and intra-observer concordance were calculated and compared (α = 0.05). RESULTS: Sharpening filters did not lead to significant differences in AUC, sensitivity, and specificity at the three mA levels tested (p > 0.05), regardless of the presence of endodontic filling (p > 0.05). However, the significant reduction of AUC observed in CBCT volumes at 4 mA without filter (p < 0.05) ceased to exist after the application of filters (p > 0.05). Sensitivity and specificity ranged from low and moderate. CONCLUSIONS: The use of sharpening filters can be recommended in CBCT volumes at 4 mA for root fracture detection for leading to the same performance as those at 6.3 and 10 mA. The presence of endodontic filling material did not influence the action of filters in the diagnosis of root fracture. CLINICAL RELEVANCE: Sharpening filters seem to contribute to the diagnosis of root fracture in CBCT volumes acquired with reduced radiation dose.

The Other US Border: Health Insurance Coverage Among Latino Immigrants In Puerto Rico.

Puerto Rico is a US territory and a popular destination for Latino immigrants in the Caribbean. Even with few language and cultural barriers, however, many Latino immigrants in Puerto Rico are uninsured. Using data from the 2014-19 Puerto Rico Community Survey, we examined inequities in health insurance coverage for non-Puerto Rican Latinos ages 18-64 living in Puerto Rico according to citizenship status and Latino subgroup (Dominican, Cuban, Mexican, and other Latino). After controlling for potential confounders, we found that noncitizen Dominicans had a significantly lower probability of having any health insurance (57.2 percent) and having any private insurance (31.5 percent). Regardless of similarities in culture and language, Latino immigrants on the island, particularly Dominicans, experience major health insurance coverage inequities. Considering that Puerto Rico's immigration system is regulated by US federal statute, both federal and local policy makers should acknowledge and focus on reducing these immigrant disparities in health insurance coverage.

Ambient urban N deposition drives increased biomass and total plant N in two native prairie grass species in the U.S. Southern Great Plains.

Remnants of native tallgrass prairie experience elevated atmospheric nitrogen (N) deposition in urban areas, with potential effects on species traits that are important for N cycling and species composition. We quantified bulk (primarily wet) inorganic N (NH4+-N + NO3--N) deposition at six sites along an urban development gradient (6-64% urban) in the Dallas-Fort Worth metropolitan area from April 2014 to October 2015. In addition, we conducted a phytometer experiment with two common native prairie bunchgrass species--one well studied (Schizachyrium scoparium) and one little studied (Nasella leucotricha)--to investigate ambient N deposition effects on plant biomass and tissue quality. Bulk inorganic N deposition ranged from 6.1-9.9 kg ha-1 yr-1, peaked in spring, and did not vary consistently with proportion of urban land within 10 km of the sites. Total (wet + dry) inorganic N deposition estimated using bulk deposition measured in this study and modeled dry deposition was 12.9-18.2 kg ha-1 yr-1. Although the two plant species studied differ in photosynthetic pathway, biomass, and tissue N, they exhibited a maximum 2-3-fold and 2-4-fold increase in total biomass and total plant N, respectively, with 1.6-fold higher bulk N deposition. In addition, our findings indicate that while native prairie grasses may exhibit a positive biomass response to increased N deposition up to ~18 kg ha-1 yr-1, total inorganic N deposition is well above the estimated critical load for herbaceous plant species richness in the tallgrass prairie of the Great Plains ecoregion and thus may negatively affect these plant communities.