Novel class of aldehyde reductases identified from Scheffersomyces stipitis for detoxification processes in cellulosic ethanol production industry.

The increase in industrialization has led to a significant energy crisis, sparking interest in lignocellulosic biomass for fuel ethanol production because of its renewable characteristics. The complex composition of this biomass requires pretreatment to reduce inhibitors like furfural and hydroxymethylfurfural (HMF), which hinder enzymatic hydrolysis and fermentation, ultimately decreasing ethanol yields. This study investigates the detoxification mechanisms of furan aldehydes in Scheffersomyces stipitis, particularly through the upregulation of genes SsOYE2.2, SsOYE2.7, and SsOYE3.1 under furfural and HMF stress. Enzyme characterization determined that SsOye3.3p is the most active enzyme for reducing both compounds using NADPH. Notably, SsOye2.6p showed the highest catalytic efficiency towards furfural, while SsOye2.8p was optimal for HMF. The study also established the optimal temperature and pH for these enzymatic reactions. Importantly, SsOye2.5p displayed broad substrate specificity, indicating its potential in detoxifying various aldehydes in microbial cells. The findings suggest that genes linked to enhanced enzymatic properties were not significantly induced, indicating that S. stipitis has more substantial potential for furan aldehyde detoxification and can be developed as a chassis organism exhibiting furan aldehyde tolerance. These insights facilitate the development of novel enzymes to counter furan aldehyde inhibitors and the creation of furan aldehyde-tolerant strains via genetic engineering.

The Effect of Low-Intensity Interval Exercise with Blood Flow Restriction on Plasma Cardiac Troponin: A Cross-Design Trial.

BACKGROUND: Low-intensity training with blood flow restriction (BFR) training could induce endurance adaptations, its impact on myocardial markers is still unclear compared to training without BFR. Consequently, the influence of low-intensity interval exercise with and without BFR and high-intensity interval exercise (HIIE) on cardiac troponin was determined in this study. METHODS: Twelve physically active males between 18 and 26 years volunteered as participants. The participants completed 3 exercise tests in random order, which included 40% VO2max low-intensity cycling without BFR (group L), 40% VO2max low-intensity cycling with BFR set at 60% limb occlusion pressure (LOP) (group B), and 80% VO2max high-intensity cycling without BFR (group H). Participant muscle oxygen, blood flow, oxygen uptake, heart rate (HR), perceived exertion (RPE) rating, and pain levels were determined before and after exercise, after cuff inflation, and pre- and post-each exercise. Moreover, before each protocol, immediately after the exercises, and 3-4 hours after each exercise, elbow vein blood samples were collected to evaluate lactate (LA) and high-sensitivity cardiac troponin T (cTnT). RESULTS: Increased LA was recorded after exercise by the individuals in group H, which was more significant than in group B. Moreover, group B documented a more significant LA increment than group L (P < .05). The peak cTnT of groups B and H after exercise was significantly higher (P < .05). Furthermore, the increase was more significant than the values recorded by group L (P < .05). CONCLUSION: The present study demonstrated that low-intensity interval exercise combined with BFR could cause cTnT elevations compared to training without BFR. The increase was similar to HIIE protocols.

Preparation and Properties of GMS/HTSF-Modified Waterborne Polyurethane Fluorine-Free Waterproof and Breathable Film.

Developing a fluorine-free, durable, high-performance waterproof breathable film for fabrics remains a formidable challenge. In this paper, a strategy for the preparation of fluorine-free, durable, and high-efficiency fabric waterproof and breathable membranes using glyceryl monostearate (GMS)/double-ended hydroxy silicone oil (HTSF)-modified waterborne polyurethane was proposed. The orderly orientation of GMS and HTSF gives the fabric excellent water-repellent properties, and the polyurethane macromolecular chain ensures strong adhesion of long-chain alkanes and silicones to the fabric surface. In this paper, the effects of different GMS contents on the stability, chemical structure, particle size, viscosity, water absorption performance, surface morphology, and XPS of a waterborne polyurethane fluorine-free waterproof and breathable membrane (GHWPU) were studied. At the same time, the application properties of GHWPU-treated fabrics, such as waterproof performance, antifouling performance, surface energy, morphology, and air permeability, were discussed. Through the analysis of SEM and XPS, it was found that the folds on the surface of the film were more and more orderly with the increasing content of GMS, and this orderly distribution of water-repellent groups endowed the film with excellent water-repellent ability. When the GMS content was 28 wt %, the finished fabrics had excellent comprehensive properties such as static contact angle of 141.6°, hydrostatic pressure of 96.7 KPa, resistance to more than 30 washes, and air permeability of 119.3 mm/s.

Mismatched Supramolecular Interactions Facilitate the Reprocessing of Super-Strong and Ultratough Thermoset Elastomers.

Thermoset elastomers have been extensively applied in many fields because of their excellent mechanical strengths and durable characteristics, such as an excellent chemical resistance. However, in the context of environmental issues, the nonrecyclability of thermosets has become a major barrier to the further development of these materials. Here, a well-tailored strategy is reported to solve this problem by introducing mismatched supramolecular interactions (MMSIs) into a covalently cross-linked poly(urethane-urea) network with dynamic acylsemicarbazide moieties. The MMSIs significantly strengthen and toughen the thermoset elastomer by effectively dissipating energy and resisting external stress. In addition, the elastomer recycling efficiency is improved 2.7-fold due to the superior reversibility of the MMSIs. The optimized thermoset elastomer features outstanding characteristics, including an ultrahigh tensile strength (110.8 MPa), an unprecedented tensile toughness (1245.2 MJ m-3), as well as remarkable resistance to chemical media, creep, and damage. Most importantly, it exhibits an extraordinary multirecyclability, and the 4th recycling efficiency remains close to 100%. This scalable method promotes the development of thermosets with both high performance and excellent recyclability, thereby providing valuable guidance for addressing the issue of nonrecyclability from a molecular design standpoint.

Genome-wide analysis of NAC transcription factors and exploration of candidate genes regulating selenium metabolism in Broussonetia papyrifera.

MAIN CONCLUSION: Genome-wide identification revealed 79 BpNAC genes belonging to 16 subfamilies, and their gene structures and evolutionary relationships were characterized. Expression analysis highlighted their importance in plant selenium stress responses. Paper mulberry (Broussonetia papyrifera), a deciduous arboreal plant of the Moraceae family, is distinguished by its leaves, which are abundant in proteins, polysaccharides, and flavonoids, positioning it as a novel feedstock. NAC transcription factors, exclusive to plant species, are crucial in regulating growth, development, and response to biotic and abiotic stress. However, extensive characterization of the NAC family within paper mulberry is lacking. In this study, 79 BpNAC genes were identified from the paper mulberry genome, with an uneven distribution across 13 chromosomes. A comprehensive, genome-wide analysis of BpNACs was performed, including investigating gene structures, promoter regions, and chromosomal locations. Phylogenetic tree analysis, alongside comparisons with Arabidopsis thaliana NACs, allowed for categorizing these genes into 16 subfamilies in alignment with gene structure and motif conservation. Collinearity analysis suggested a significant homologous relationship between the NAC genes of paper mulberry and those in Morus notabilis, Ficus hispida, Antiaris toxicaria, and Cannabis sativa. Integrating transcriptome data and Se content revealed that 12 BpNAC genes were associated with selenium biosynthesis. Subsequent RT-qPCR analysis corroborated the correlation between BpNAC59, BpNAC62 with sodium selenate, and BpNAC55 with sodium selenite. Subcellular localization experiments revealed the nuclear functions of BpNAC59 and BpNAC62. This study highlights the potential BpNAC transcription factors involved in selenium metabolism, providing a foundation for strategically breeding selenium-fortified paper mulberry.

Revealing the reconstruction mechanism of AgPd nanoalloys under fluorination based on a multiscale deep learning potential.

The design of heterogeneous catalysts generally involves optimizing the reactivity descriptor of adsorption energy, which is inevitably governed by the structure of surface-active sites. A prerequisite for understanding the structure-properties relationship is the precise identification of real surface-active site structures, rather than relying on conceived structures derived from bulk alloy properties. However, it remains a formidable challenge due to the dynamic nature of nanoalloys during catalytic reactions and the lack of accurate and efficient interatomic potentials for simulations. Herein, a generalizable deep-learning potential for the Ag-Pd-F system is developed based on a dataset encompassing the bulk, surface, nanocluster, amorphous, and point defected configurations with diverse compositions to achieve a comprehensive description of interatomic interactions, facilitating precise prediction of adsorption energy, surface energy, formation energy, and diffusion energy barrier and is utilized to investigate the structural evolutions of AgPd nanoalloys during fluorination. The structural evolutions involve the inward diffusion of F, the outward diffusion of Ag in Ag@Pd nanoalloys, the formation of surface AgFx species in mixed and Janus AgPd nanoalloys, and the shape deformation from cuboctahedron to sphere in Ag and Pd@Ag nanoalloys. Moreover, the effects of atomic diffusion and dislocation formation and migration on the reconstructing pathway of nanoalloys are highlighted. It is demonstrated that the stress relaxation upon F adsorption serves as the intrinsic driving factor governing the surface reconstruction of AgPd nanoalloys.

Selective Excitation of Exciton-Polariton Condensate Modes in an Annular Perovskite Microcavity.

Exciton-polariton systems composed of a light-matter quasi-particle with a light effective mass easily realize Bose-Einstein condensation. In this work, we constructed an annular trap in a halide perovskite semiconductor microcavity and observed the spontaneous formation of symmetrical petal-shaped exciton-polariton condensation in the annular trap at room temperature. In our study, we found that the number of petals of the petal-shaped exciton-polariton condensates, which is decided by the orbital angular momentum, is dependent on the light intensity distribution. Therefore, the selective excitation of perovskite microcavity exciton-polariton condensates under all-optical control can be realized by adjusting the light intensity distribution. This could pave the way to room-temperature topological devices, optical cryptographical devices, and new quantum gyroscopes in the exciton-polariton system.

Genome-wide identification of HMT gene family explores BpHMT2 enhancing selenium accumulation and tolerance in Broussonetia papyrifera.

Broussonetia papyrifera, a valuable feed resource, is known for its fast growth, wide adaptability, high protein content and strong selenium enrichment capacity. Selenomethionine (SeMet), the main selenium form in selenium fortification B. papyrifera, is safe for animals and this enhances its nutritional value as a feed resource. However, the molecular mechanisms underlying SeMet synthesis remain unclear. This study identified three homocysteine S-methyltransferase genes from the B. papyrifera genome. The phylogenetic tree demonstrated that BpHMTs were divided into two classes, and BpHMT2 in the Class 2-D subfamily evolved earlier and possesses more fundamental functions. On the basis of the correlation between gene expression levels and selenium content, BpHMT2 was identified as a key candidate gene associated with selenium tolerance. Subcellular localization experiments confirmed the targeting of BpHMT2 in nucleus, cell membrane and chloroplasts. Moreover, three BpHMT2 overexpression Arabidopsis thaliana lines were confirmed to enhance plant selenium tolerance and SeMet accumulation. Overall, our finding provides insights into the molecular mechanisms of selenium metabolism in B. papyrifera, highlighting the potential role of BpHMT2 in SeMet synthesis. This research contributes to our understanding of selenium-enriched feed resources, with increased SeMet content contributing to the improved nutritional value of B. papyrifera as a feed resource.

The impact of digital healthcare systems on pain and body function in patients with knee joint pain: a systematic review and meta-analysis.

The digital healthcare (DH) system has recently emerged as an advanced rehabilitation approach that promotes rehabilitation training based on virtual reality (VR) and augmented reality (AR). The purpose of this meta-analysis study is to review and assess the impact of DH systems on pain and physical function among patients diagnosed with knee joint pain. Between January 2003 and September 2023, studies that met the listed inclusion criteria were gathered from Scopus, PubMed, Web of Science, and EBSCO databases. The analysis of standardized mean difference (SMD) was carried out with 95% confidence interval (95% CI) (PROSPERO registration number: CRD42023462538). Eight research papers were selected, which collectively involved 194 males and 279 females. The meta-analysis outcomes revealed that DH intervention significantly improved balance (SMD, 0.41 [0.12, 0.69], p < 0.05) and pain level (SMD, - 1.10 [- 2.02, - 0.18], p < 0.05). The subgroup analysis of the pain level showed varied outcomes for the TKA (SMD, - 0.22 [- 0.49, 0.04], p = 0.10) or OA patients (SMD, - 2.80 [- 3.83, - 1.78], p < 0.05) Next, this study found no significant effect of DH intervention on knee joint range of motion (ROM) (SMD, 0.00 [- 0.76, 0.76], p = 1.00) and walking velocity (SMD, 0.04 [- 0.22, 0.29], p = 0.77) in patients with knee joint pain. The meta-analysis review conducted in this study revealed that DH intervention may potentially improve balance among the patients with knee joint pain. It may also alleviate the pain level particularly among OA patients.

Ab Initio Molecular Dynamics Study of the Proton Transfer in Hydroxyl Ion-Induced Hydrolysis of Aluminum Monomers.

The hydrolysis process of Al(H2O)63+ induced by hydroxyl ions (OH-) is significant to aluminum solution chemistry. Previous investigations of hydrolysis reactions have primarily relied on static calculations in an implicit solvent environment. Herein, we employ ab initio molecular dynamics (AIMD) to investigate the evolution process of Al(H2O)63+ under various local alkaline conditions in an explicit solvent environment. Our work demonstrates the effect of solvent water in hydrolysis reactions. Specifically, the stepwise hydrolysis reaction induced by hydroxyl ions involves water wire compression and concerted proton transfers. Dehydration reactions occur when the number of hydroxyl ligands attached to the aluminum ion (Al3+) equals or exceeds three. Moreover, the Al(H2O)n(OH)3 species exhibit unique hydrolysis and dehydration reaction characteristics compared to other species. The geometrically stable aluminum monomers determined by AIMD are Al(H2O)5(OH)12+, Al(H2O)4(OH)2+, Al(H2O)1(OH)3, and Al(OH)4-. In addition, the topological analysis analyzes the interaction between Al3+ and coordinated H2O in different configurations, indicating the weakest interaction appearing in Al(H2O)n(OH)3 species.

The mechanisms of minocycline in alleviating ischemic stroke damage and cerebral ischemia-reperfusion injury.

Stroke is a group of diseases resulting from cerebral vascular rupture or obstruction and subsequent brain blood circulation disorder, leading to rapid neurological deficits. Ischemic stroke accounts for the majority of all stroke cases. The current treatments for ischemic stroke mainly include t-PA thrombolytic therapy and surgical thrombectomy. However, these interventions aimed at recanalizing cerebral vessels can paradoxically lead to ischemia-reperfusion injury, which exacerbates the severity of brain damage. Minocycline, a semi-synthetic tetracycline antibiotic, has been shown to possess a wide range of neuroprotective effects independent of its antibacterial activity. Here we summarize the mechanisms underlying the protective effects of minocycline against cerebral ischemia-reperfusion injury based on the pathogenesis of cerebral ischemia-reperfusion injury, including its modulation of oxidative stress, inflammatory response, excitotoxicity, programmed cell death and blood-brain barrier injury, and also introduce the role of minocycline in alleviating stroke-related complications, in order to provide a theoretical basis for the clinical application of minocycline in cerebral ischemia-reperfusion injury.

Structural transformations in single-crystalline AgPd nanoalloys from multiscale deep potential molecular dynamics.

AgPd nanoalloys often undergo structural evolution during catalytic reactions; the mechanism underlying such restructuring remains largely unknown due to the use of oversimplified interatomic potentials in simulations. Herein, a deep-learning potential is developed for AgPd nanoalloys based on a multiscale dataset spanning from nanoclusters to bulk configurations, exhibits precise predictions of mechanical properties and formation energies with near-density functional theory accuracy, calculates the surface energies closer to experimental values compared to those obtained by Gupta potentials, and is applied to investigate the shape reconstruction of single-crystalline AgPd nanoalloys from cuboctahedron (Oh) to icosahedron (Ih) geometries. The Oh to Ih shape restructuring is thermodynamically favorable and occurs at 11 and 92 ps for Pd55@Ag254 and Ag147@Pd162 nanoalloys, respectively. During the shape reconstruction of Pd@Ag nanoalloys, concurrent surface restructuring of the (100) facet and internal multi-twinned phase change are observed with collaborative displacive characters. The presence of vacancies can influence the final product and reconstructing rate of Pd@Ag core-shell nanoalloys. The Ag outward diffusion on Ag@Pd nanoalloys is more pronounced in Ih geometry compared to Oh geometry and can be further accelerated by the Oh to Ih deformation. The deformation of single-crystalline Pd@Ag nanoalloys is characterized by a displacive transformation involving the collaborative displacement of a large number of atoms, distinguishing it from the diffusion-coupled transformation of Ag@Pd nanoalloys.

Atomic strain and catalytic properties of formate oxidation and dehydrogenation in AgPd nanoalloys.

Formate is a promising hydrogen carrier for safe storage and transport and a fuel for direct formate fuel cells. However, the sluggish kinetics of catalysts for formate dehydrogenation (FDH) and oxidation reactions (FORs) significantly limit the potential applications of formate. Strain effects can effectively modulate catalytic properties by altering the electronic structure. Nevertheless, the lack of theoretical principles to quantify atomic strain and its effects on FDH and FOR catalytic activity has made experimental efforts laborious. In this work, we establish a database of atomic strain distributions for AgPd nanoalloys, reveals that the presence of compressive strain at the edges and corners and compressive strain exerted on the surface of Ag@Pd nanoalloys, particularly the one with an icosahedral shape, boost the FDH and FOR catalytic activity by shifting down the d-band center, thus weakening the adsorption of key intermediate Had. This study provides a theoretical perspective on the development and use of formate as a hydrogen carrier and fuel.

Diode Laser Enucleation vs. Bipolar Transurethral Enucleation of Prostate for Benign Prostatic Hyperplasia: A Retrospective Comparative Study with Three-Year Follow Up.

OBJECTIVE: To assess the clinical outcome of 1470 nm diode laser enucleation of the prostate (DiLEP) vs. bipolar transurethral enucleation of prostate (TUEP) for benign prostatic hyperplasia (BPH). METHODS: One hundred and fifty-seven patients were retrospectively involved in this study. Eighty-two patients underwent DiLEP while 75 underwent bipolar TUEP. Seventy-three patients in DiLEP and sixty-nine in bipolar TUEP completed the 3-year follow-up, respectively. The baseline properties, perioperative data, and postsurgical outcomes were evaluated. RESULTS: No statistically significant differences were found between DiLEP and bipolar TUEP in preoperative parameters. Significantly shorter operating time was noted in DiLEP group (p = 0.000). No patient suffered dangerous complications, and none in either group required a blood transfusion. No statistically significant differences were found between DiLEP and bipolar TUEP in the decrease in hemoglobin or sodium. During the 3-year postoperative follow-up, ongoing and significant improvements were found in both groups without any difference. CONCLUSIONS: Both DiLEP and bipolar TUEP can improve low urinary tract symptoms (LUTS) secondary to BPH in a comparable way with high efficacy. Compared with bipolar TUEP, DiLEP with a morcellator required a shorter operative time.

Development of Tough Thermoplastic Elastomers by Leveraging Rigid-Flexible Supramolecular Segment Interplays.

Supramolecular interactions facilitate the development of tough multifunctional thermoplastic elastomers. However, the fundamental principles that govern supramolecular toughening are barely understood, and the rational design to achieve the desired high toughness remains daunting. Herein, we report a simple and robust method for toughening thermoplastic elastomers by rationally tailoring hard-soft phase separation structures containing rigid and flexible supramolecular segments. The introduced functional segments with distinct structural rigidities provide mismatched supramolecular interactions to efficiently tune the energy dissipation and bear an external load. The optimal supramolecular elastomer containing aromatic amide and acylsemicarbazide moieties demonstrates a record toughness (1.2 GJ m-3 ), extraordinary crack tolerance (fracture energy 282.5 kJ m-2 ), an ultrahigh true stress at break (2.3 GPa), good elasticity, healing ability, recyclability, and impact resistance. The toughening mechanism is validated by testing various elastomers, confirming the potential for designing and developing super-tough supramolecular materials with promising applications in aerospace and electronics.

Predicting the comprehensive geospatial pattern of two ephedrine-type alkaloids for Ephedra sinica in Inner Mongolia.

Ephedra sinica Stapf. is a shrubby plant widely used in traditional Chinese medicine due to its high level of medicinal value, thus, it is in high demand. Ephedrine (E) and pseudoephedrine (PE) are key medicinal components and quality indicators for E. sinica. These two ephedrine-type alkaloids are basic elements that exert the medicinal effect of E. sinica. Recently, indiscriminate destruction and grassland desertification have caused the quantity and quality of these pharmacological plants to degenerate. Predicting potentially suitable habitat for high-quality E. sinica is essential for its future conservation and domestication. In this study, MaxEnt software was utilized to map suitable habitats for E. sinica in Inner Mongolia based on occurrence data and a set of variables related to climate, soil, topography and human impact. The model parametrization was optimized by evaluating alternative combinations of feature classes and values of the regularization multiplier. Second, a geospatial quality model was fitted to relate E and PE contents to the same environmental variables and to predict their spatial patterns across the study area. Outputs from the two models were finally coupled to map areas predicted to have both suitable conditions for E. sinica and high alkaloid content. Our results indicate that E. sinica with high-quality E content was mainly distributed in the Horqin, Ulan Butong and Wulanchabu grasslands. E. sinica with high-quality PE content was primarily found in the Ordos, Wulanchabu and Ulan Butong grasslands. This study provides scientific information for the protection and sustainable utilization of E. sinica. It can also help to control and prevent desertification in Inner Mongolia.

Nickel -supported PdM (M = Au and Ag) nanodendrites as formate oxidation (electro)catalytic anodes for direct fuel cells and hydrogen generation at room temperature.

The study provides a proof of concept for the first time that unique palladium-gold (PdAu) and palladium-silver (PdAg) nanodendrites are bifunctional catalytic active sites for formate oxidation reactions (FORs) and formate dehydrogenation reactions (FDRs). The unique nanodendritic structure was developed via a simple galvanic displacement reaction for the direct growth of PdAu and PdAg nanodendrites on a nickel foam (PdAu/NiNF and PdAg/NiNF). These PdAu/NiNF and PdAg/NiNF electrodes exhibited 2.32 and 1.59 times higher specific activity than that of the commercial Pd/C electrode and promising stability toward FORs. Moreover, the PdAu/NiNF and PdAg/NiNF nanodendrites were also highly active and selective catalysts for hydrogen generation from a formate solution with turnover frequency (TOF) values of 311 h-1 and 287 h-1 respectively. Impressively, a passive air-breathing formate fuel cell with PdAu/NiNF used as an anode can yield an open-circuit voltage of 1.12 V and a peak power density of 21.7 mW cm-2, which outperforms most others reported in the literature. PdAu and PdAg nanodendritic catalysts supported on a nickel foam demonstrate an open structure and uniform catalyst distribution and offer a promising nanoalloy for air-breathing formate fuel cells and on-site chemical hydrogen production systems.

Modeling for Predicting the Potential Geographical Distribution of Three Ephedra Herbs in China.

Ephedra species are beneficial for environmental protection in desert and grassland ecosystems. They have high ecological, medicinal, and economic value. To strengthen the protection of the sustainable development of Ephedra, we used occurrence records of Ephedra sinica Stapf., Ephedra intermedia Schrenk et C.A. Mey., and Ephedra equisetina Bge., combined with climate, soil, and topographic factors to simulate the suitable habitat of three Ephedra based on ensemble models on the Biomod2 platform. The results of the models were tested using AUC, TSS, and kappa coefficients. The results demonstrated that the ensemble model was able to accurately predict the potential distributions of E. sinica, E. intermedia, and E. equisetina. Eastern and central Inner Mongolia, middle and eastern Gansu, and northeastern Xinjiang were the optimum regions for the growth of E. sinica, E. intermedia, and E. equisetina, respectively. Additionally, several key environmental factors had a significant influence on the suitable habitats of the three Ephedra. The key factors affecting the distribution of E. sinica, E. intermedia, and E. equisetina were annual average precipitation, altitude, and vapor pressure, respectively. In conclusion, the results showed that the suitable ranges of the three Ephedra were mainly in Northwest China and that topography and climate were the primary influencing factors.

Dual-Function Detoxifying Nanofabrics against Nerve Agent and Blistering Agent Simulants.

The development of functional materials that can detoxify multiple chemical warfare agents (CWAs) at the same time is of great significance to cope with the uncertainty of CWA use in real-world situations. Although many catalysts capable of detoxifying CWAs have been reported, there is still a lack of effective means to integrate these catalytic-active materials on practical fibers/fabrics to achieve effective protection against coexistence of a variety of CWAs. In this work, by a combination of electrospinning and in situ solvothermal reaction, PAN@Zr(OH)4@MOF-808 nanofiber membranes were prepared for detoxification of both nerve agent and blistering agent simulants dimethyl 4-nitrophenyl phosphate (DMNP) and 2-chloroethyl ethyl sulfide (CEES). Under the catalytic effect of the MOF-808 component, DMNP hydrolysis with a half-life as short as 1.19 min was achieved. Meanwhile, an 89.3% CEES removal rate was obtained within 12 h by adsorption and catalysis of MOF-808 and Zr(OH)4 components at ambient conditions, respectively. PAN@Zr(OH)4@MOF-808 nanofiber membranes also showed a superior blocking effect on CEES compared to bare PAN and PAN@Zr(OH)4 nanofiber membranes. Simultaneous protection against DMNP and CEES showed effective inhibition of both simulants for at least 2 h. The preparation method also imparted intrinsically good interfacial adhesion between the components, contributing to the excellent recycling stability of PAN@Zr(OH)4@MOF-808 nanofiber membranes. Therefore, the prepared composite nanofabrics have great application potential, which provides a new idea for the construction of broad-spectrum protective detoxification materials.