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1.
Heliyon ; 10(16): e35960, 2024 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-39224262

RESUMO

Antisense long non-coding RNA (AS-lncRNA) represents a novel class of RNA molecules. In recent years, it has been discovered that AS-lncRNAs play crucial roles in various biological processes, particularly in the onset and progression of tumors. Skull base tumors, originating from the base of the brain, exhibit specific expression patterns of AS-lncRNA which correlate significantly with clinical characteristics. This makes AS-lncRNA a promising candidate as a tumor marker. Functional studies have revealed that AS-lncRNAs can regulate gene expression by acting as miRNA sponges and interacting with RBPs. Consequently, they play pivotal roles in tumor cell cycle, apoptosis, angiogenesis, invasion, and metastasis processes. Further exploration into the mechanisms of AS-lncRNA in tumors holds substantial theoretical significance for deeper insights into the etiology, pathogenesis, and RNA dynamics of skull base tumors. Moreover, AS-lncRNA could serve as molecular markers or potential targets for early diagnosis. Their potential extends to efficacy assessment, prognosis prediction, and gene therapy, suggesting broad clinical applications. In summary, AS-lncRNA emerges as a promising molecular marker implicated in the onset and progression of skull base tumors.

2.
J Med Chem ; 67(13): 10687-10709, 2024 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-38913701

RESUMO

UC and ALI are inflammatory diseases with limited treatment in the clinic. Herein, fragment-based anti-inflammatory agent designs were carried out deriving from cyclohexylamine/cyclobutylamine and several fragments from anti-inflammatory agents in our lab. AF-45 (IC50 = 0.53/0.60 µM on IL-6/TNF-α in THP-1 macrophages) was identified as the optimal molecule using ELISA and MTT assays from the 33 synthesized compounds. Through mechanistic studies and a systematic target search process, AF-45 was found to block the NF-κB/MAPK pathway and target IRAK4, a promising target for inflammation and autoimmune diseases. The selectivity of AF-45 targeting IRAK4 was validated by comparing its effects on other kinase/nonkinase proteins. In vivo, AF-45 exhibited a good therapeutic effect on UC and ALI, and favorable PK proprieties. Since there are currently no clinical or preclinical trials for IRAK4 inhibitors to treat UC and ALI, AF-45 provides a new lead compound or candidate targeting IRAK4 for the treatment of these diseases.


Assuntos
Lesão Pulmonar Aguda , Colite Ulcerativa , Quinases Associadas a Receptores de Interleucina-1 , Quinases Associadas a Receptores de Interleucina-1/antagonistas & inibidores , Quinases Associadas a Receptores de Interleucina-1/metabolismo , Humanos , Animais , Colite Ulcerativa/tratamento farmacológico , Lesão Pulmonar Aguda/tratamento farmacológico , Lesão Pulmonar Aguda/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Inibidores de Proteínas Quinases/síntese química , Inibidores de Proteínas Quinases/química , Inibidores de Proteínas Quinases/uso terapêutico , Desenho de Fármacos , Camundongos , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Anti-Inflamatórios/síntese química , Anti-Inflamatórios/química , Descoberta de Drogas , Masculino , NF-kappa B/metabolismo , NF-kappa B/antagonistas & inibidores , Relação Estrutura-Atividade , Células THP-1
3.
Eur J Med Chem ; 272: 116487, 2024 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-38759452

RESUMO

Acute lung injury (ALI) and inflammatory bowel disease (IBD) are common inflammatory illnesses that seriously affect people's health. Herein, a series of 4-hydroxylcoumarin (4-HC) derivatives were designed and synthesized. The inhibitory effects of these compounds on LPS-induced interleukin-6 (IL-6) release from J774A.1 cells were then screened via ELISA assay, compound B8 showed 3 times more active than the lead compound 4-HC. The most active compound B8 had the IC50 values of 4.57 µM and 6.51 µM for IL-6 release on mouse cells J774A.1 and human cells THP-1, respectively. Furthermore, we also found that B8 could act on the MAPK pathway. Based on the target prediction results of computer virtual docking, kinase inhibitory assay was carried out, and it revealed that targeting IRAK1 was a key mechanism for B8 to exert anti-inflammatory activity. Moreover, B8 exerted a good therapeutic effect on the dextran sulfate sodium (DSS)-induced colitis model and liposaccharide (LPS)-induced ALI mouse models. The acute toxicity experiments indicated that high-dose B8 caused no adverse reactions in mice, confirming its safety in vivo. Additionally, the preliminary pharmacokinetic (PK) parameters of B8 in SD rats were also examined, revealing a bioavailability (F) of 28.72 %. In conclusion, B8 is a potential candidate of drug for the treatment of ALI and colitis.


Assuntos
4-Hidroxicumarinas , Lesão Pulmonar Aguda , Colite , Desenho de Fármacos , Lesão Pulmonar Aguda/tratamento farmacológico , Lesão Pulmonar Aguda/induzido quimicamente , Animais , Colite/tratamento farmacológico , Colite/induzido quimicamente , Camundongos , Humanos , Relação Estrutura-Atividade , 4-Hidroxicumarinas/farmacologia , 4-Hidroxicumarinas/química , 4-Hidroxicumarinas/síntese química , Estrutura Molecular , Sulfato de Dextrana , Masculino , Relação Dose-Resposta a Droga , Ratos , Lipopolissacarídeos/farmacologia , Lipopolissacarídeos/antagonistas & inibidores , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/síntese química , Anti-Inflamatórios/química , Anti-Inflamatórios/uso terapêutico , Anti-Inflamatórios não Esteroides/farmacologia , Anti-Inflamatórios não Esteroides/síntese química , Anti-Inflamatórios não Esteroides/química , Interleucina-6/metabolismo , Interleucina-6/antagonistas & inibidores , Quinases Associadas a Receptores de Interleucina-1/antagonistas & inibidores , Quinases Associadas a Receptores de Interleucina-1/metabolismo , Simulação de Acoplamento Molecular , Camundongos Endogâmicos C57BL , Linhagem Celular
4.
Eur J Med Chem ; 268: 116252, 2024 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-38422703

RESUMO

The modification based on natural products is a practical way to find anti-inflammatory drugs. In this study, 26 osthole derivatives were synthesized, and their anti-inflammatory properties were evaluated. The preliminary activity study revealed that most osthole derivatives could effectively inhibit inflammatory cytokines IL-6 secretion in LPS stimulated mouse macrophages J774A.1. Compound 7m exhibited the most effective anti-inflammatory activity (RAW264.7 IL-6 IC50: 4.57 µM, 32 times more active than osthole) in vitro with no significant influence on cell proliferation. Additionally, the mechanistic analysis demonstrated that compound 7m could block MAPK signal transduction by inhibiting the phosphorylation of JNK and p38, thereby inhibiting the release of inflammatory cytokines. Moreover, in vivo functional investigations revealed that 7m could substantially reduce DSS-induced ulcerative colitis and LPS-induced acute lung injury, with good therapeutic effects. The pharmacokinetics and acute toxicity experiments proved the safety and reliability of 7min vivo. Overall, Compound 7m could further be studied as potential anti-inflammatory candidate.


Assuntos
Lesão Pulmonar Aguda , Colite Ulcerativa , Colite , Cumarínicos , Animais , Camundongos , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/tratamento farmacológico , Lipopolissacarídeos/farmacologia , Interleucina-6 , Reprodutibilidade dos Testes , Anti-Inflamatórios/efeitos adversos , Lesão Pulmonar Aguda/induzido quimicamente , Lesão Pulmonar Aguda/tratamento farmacológico , Citocinas , NF-kappa B , Camundongos Endogâmicos C57BL , Colite/tratamento farmacológico
5.
Eur J Med Chem ; 259: 115706, 2023 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-37572538

RESUMO

The uneven regulation of inflammation is related to various diseases, making anti-inflammation a potential option for the development of novel therapies. In this study, we designed and synthesized a total of fifty-eight novel amide/sulfonamide derivatives based on our previously reported anti-inflammatory compounds. The anti-inflammatory activities of these compounds were evaluated upon LPS-stimulated J774A.1 cells. Compounds 11a, 11b, 11c, and 11d potently reduced the release of IL-6 and TNF-α, and decreased the mRNA level of cytokines in J774A.1 cells. The most active compound 11d with IC50 value of 0.61 µM for IL-6 inhibition, and 4.34 µM for TNF-α inhibition restored IκB α and inhibited the translocation of phosphorylated p65 into the nucleus. In vivo evaluation indicated that 11d improved LPS-induced ALI and alleviated DSS-induced ulcerative colitis in mice. In conclusion, these results suggested compound 11d can be a new lead structure for the development of anti-inflammatory drugs against ALI and ulcerative colitis.


Assuntos
Lesão Pulmonar Aguda , Colite Ulcerativa , Camundongos , Animais , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/tratamento farmacológico , Fator de Necrose Tumoral alfa , Amidas/farmacologia , Amidas/uso terapêutico , Lipopolissacarídeos/farmacologia , Interleucina-6 , Anti-Inflamatórios/efeitos adversos , Citocinas , Lesão Pulmonar Aguda/tratamento farmacológico , Sulfonamidas/uso terapêutico , NF-kappa B
6.
J Med Chem ; 66(17): 12304-12323, 2023 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-37643372

RESUMO

Acute lung injury (ALI) and sepsis are both serious and complex conditions associated with high mortality, yet there are no effective treatments. Herein, we designed and synthesized a series of diphenyl 6-oxo-1,6-dihydropyridazine-3-carboxylate/carboxamide analogues exhibiting anti-inflammatory activity. The optimal compound J27 decreased the release of TNF-α and IL-6 in mouse and human cells J774A.1 and THP-1 (IL-6 IC50 = 0.22 µM) through the NF-κB/MAPK pathway. J27 demonstrated remarkable protection against ALI and sepsis in vivo and exhibited good safety in subacute toxicity experiments. Pharmacokinetic study indicated that J27 had good bioavailability (30.74%). To our surprise, J27 could target JNK2 with a totally new molecular skeleton compared with the only few JNK2 inhibitors reported. Moreover, there is no report that JNK2 inhibitors could apply for ALI and sepsis. Therefore, this work provides a new lead structure for the study of JNK2 inhibitors and a new target of JNK2 to treat ALI and sepsis.


Assuntos
Lesão Pulmonar Aguda , Sepse , Humanos , Animais , Camundongos , NF-kappa B , Interleucina-6 , Sepse/tratamento farmacológico , Lesão Pulmonar Aguda/tratamento farmacológico , Ácidos Carboxílicos
7.
Neuropsychiatr Dis Treat ; 19: 153-169, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36698699

RESUMO

Objective: T cell immunoglobulin and mucin domain-3 (Tim-3) may be implicated in neuroinflammation. Herein, we attempted to discern the role of serum soluble (s) Tim-3 as an inflammatory prognostic biomarker of severe traumatic brain injury (sTBI). Methods: In this prospective observational study of 112 sTBI patients and 112 controls, serum sTim-3 levels were determined, Rotterdam computed tomography (CT) classification and Glasgow coma scale (GCS) were selected as the two severity indicators, serum C-reactive protein (CRP) was regarded as an inflammatory biomarker, and poor prognosis was referred to as extended Glasgow outcome scale (GOSE) scores 1-4 at 180 days after trauma. Results: Serum sTim-3 levels were markedly higher in patients than in controls (median, 4.2 ng/mL versus 0.7 ng/mL; P<0.001). Serum sTim-3 levels of patients were independently related to Rotterdam CT scores (ß=1.126), GCS scores (ß=-0.589), serum CRP levels (ß=0.155) and GOSE scores (ß=-0.211). Serum sTim-3 appeared as an independent predictor of post-traumatic 180-day mortality (odds ratio=1.289), overall survival (hazard ratio=1.208) and poor prognosis (odds ratio=1.293). Serum sTim-3 levels discriminated patients at risk of post-injury 180-day mortality and poor prognosis with areas under curve (AUCs) at 0.753 and 0.782, respectively. Serum sTim-3 levels combined with GCS scores and Rotterdam CT scores (AUC=0.869) exhibited significantly higher AUC than Rotterdam CT scores (P=0.026), but not than GCS scores (P=0.181) for death prediction and their combination (AUC=0.895) had significantly higher AUC than GCS scores (P=0.036) or Rotterdam CT scores (P=0.005) for outcome prediction. Conclusion: Elevated serum sTim-3 levels, in close correlation with traumatic severity and inflammation, are substantially associated with long-term death and poor outcome, indicating that serum sTim-3, as an inflammatory biomarker, may be of clinical significance in severity assessment and prediction of prognosis following sTBI.

8.
Huan Jing Ke Xue ; 44(1): 502-511, 2023 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-36635838

RESUMO

To illuminate the ecological functions of root-associated fungi (RAF) and their interactions with host plants, we revealed the root-associated fungal diversity and community compositions of Pinus sylvestris var. mongolica involving natural forests and plantations (half-mature, nearly mature, and mature forests) in the Hulunbuir Desert, Horqin Desert, and Mu Us Desert and investigated the environmental driving factors (climatic condition and soil property). The results indicated that: 1 the diversity of RAF in the natural forests was significantly lower than that in plantations (P<0.05), and the values were highest in the Mu Us Desert. There was a distinct geographical distribution in the RAF community, but the influence of stand age was not significant (P>0.05). 2 The relative abundance of ectomycorrhizal fungi (50.49%) in natural forests was higher than that in plantations, such as Acephala, Mycena, and Suillus. The indicator genera were diverse involving the natural forests (Acephala) and plantations in the Hulunbuir Desert (Sarcodon), Horqin Desert (Russula and Calostoma), and Mu Us Desert (Geopora, Mallocybe, and Tomentella). 3 The indicator genera were mainly affected by available nitrogen content, available phosphorus content, and stand age, and few indicator genera were related to soil water content, pH, and total nitrogen content. A total of 43.25% of the variation in the RAF community was accounted for by both geographic location and environmental factors. Overall, geographic location and environmental factors shaped the spatial variation in the RAF structure and function of P. sylvestris natural forests and plantations in the semi-arid and dry sub-humid desertified regions; there were no significant temporal variations in RAF across stand ages, but the nonuniformity in fungal distribution with stand ageing cannot be ignored. The large population of symbiotic fungi in natural forests was conducive to the healthy growth of hosts; the ratio of symbiotic, saprophytic, and pathotrophic fungi varied in different plantations, and the increase in the proportion of saprophytic and pathotrophic fungi may have negative effects on the growth and health of plantations. This improved information will provide a theoretical basis for the management of P. sylvestris plantations.


Assuntos
Micorrizas , Pinus sylvestris , Pinus , China , Solo/química , Nitrogênio/análise
9.
Mol Biotechnol ; 65(4): 612-623, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36109428

RESUMO

Advanced proliferative diabetic retinopathy (PDR) characterized by aberrant retinal angiogenesis is a leading cause of retinal detachment and blindness. Krüppel-like factor 9 (KLF9), a member of the zinc-finger family of transcription factors, participates in the development of diabetic nephropathy and the promotion of angiogenesis of human umbilical vein endothelial cells. Therefore, we speculate that KLF9 may exert a crucial role in PDR. The current study revealed that KLF9 was highly expressed in the high glucose (HG)-treated human retinal microvascular endothelial cells (HRMECs) and the retinas of oxygen-induced retinopathy (OIR) rats. Knockdown of KLF9 inhibited the proliferation, migratory capability, invasiveness and tube formation of HG-treated HRMECs. Besides, knockdown of KLF9 decreased the expression of yes-associated protein 1 (YAP1) in HG-treated HRMECs. Dual-luciferase reporter assays confirmed that KLF9 transcriptionally upregulated YAP1 expression. Overexpression of YAP1 reversed the KLF9 silencing-induced repression of HRMEC proliferation and tube formation. Further in vivo evidence demonstrated that knockdown of KLF9 reduced the expression of Ki67, CD31 and vascular endothelial growth factor A (VEGFA) in the retinas of OIR rats. Collectively, KLF9 silencing might mitigate the progression of PDR by inhibiting angiogenesis via blocking YAP1 transcription.


Assuntos
Diabetes Mellitus , Retinopatia Diabética , Humanos , Ratos , Animais , Retinopatia Diabética/genética , Retinopatia Diabética/metabolismo , Fator A de Crescimento do Endotélio Vascular/genética , Células Endoteliais/metabolismo , Retina/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Fatores de Transcrição Kruppel-Like/genética , Fatores de Transcrição Kruppel-Like/metabolismo , Proliferação de Células , Diabetes Mellitus/metabolismo
10.
Plant Physiol Biochem ; 190: 174-183, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36116226

RESUMO

'Docteur Jules Guyot' pears were immersed in acibenzolar-S-methyl (ASM) and 0.01 mol L-1 ethyl glycol tetra acetic acid (EGTA) to investigate the changes of Ca2+ receptor proteins and phenylpropanoid pathway. Results showed that ASM treatment increased the activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate coenzyme A ligase (4CL), polyphenol oxidase (PPO), and cinnamyl alcohol dehydrogenase (CAD) in the exocarp of pears, whereas EGTA pre-treatment inhibited the activities of these enzymes. ASM treatment also enhanced the transcription of PcPAL, PcC4H, Pc4CL, PcC3H, PcCOMT, PcCCoAOMT, PcCCR, PcPOD, PcCDPK1, PcCDPK2, PcCDPK5, PcCDPK11, PcCDPK13, PcCBL1, PcCBL9, PcCIPK14, and PcCML27 in pears. EGTA + ASM treatments inhibited the transcription of PcPAL, PcC4H, Pc4CL, PcC3H, PcCCR, PcF5H, PcCAD, PcCDPK11, PcCDPK26, PcCDPK32, PcCBL1, PcCIPK14, PcCIPK23, and PcCaM in the fruit. All these results indicated that ASM induced the gene expressions of Ca2+ receptor proteins, the key enzyme activities and gene expressions in phenylpropanoid pathway; Ca2+ mediated phenylpropane metabolism in pears after ASM treatment.


Assuntos
Pyrus , Cálcio , Catecol Oxidase , Cinamatos , Coenzima A Ligases/metabolismo , Ácido Egtázico , Glicóis , Lignina/genética , Fenilalanina Amônia-Liase/metabolismo , Pyrus/metabolismo , Tiadiazóis , Transcinamato 4-Mono-Oxigenase/metabolismo
11.
Food Res Int ; 160: 111741, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36076423

RESUMO

Apples (cv. Golden Delicious) were used as the materials to investigate methyl jasmonate (MeJA) dipping on quality parameters, organic acids metabolism and GABA shunt during storage at 21 ± 1 °C and 75 ± 5 % relative humidity. Results demonstrated that MeJA treatment reduced mass loss, respiratory intensity and ethylene release, and maintained higher flesh firmness and soluble solid content of apples. MeJA also decreased malic acid content, increased succinic and tartaric acids contents, and inhibited cytoplasmic aconitase (Cyt-ACO), NADP-malate (NADP-ME), phosphoenolpyruvate dehydrogenase (PEPC), mitochondrial citrate synthase (Mit-CS), glutamate dehydrogenase (GAD), and GABA transferase (GABA-T) activities in apples. NADP-isocitrate dehydrogenase (NADP-IDH), mitochondrial cis-aconitase (Mit-ACO), and cytoplasmic NAD-malate dehydrogenase (CytNAD-MDH) activities in apples were also enhanced by MeJA dipping. Moreover, MeJA dipping enhanced MdCytNAD-MDH and MdNADP-IDH expressions, and down-regulated MdGAD, MdGABA-T, MdNADP-ME, MdPEPC, MdCyt-ACO and MdMit-CS expressions in apples. These results suggest that MeJA dipping can maintain storage quality of "Golden Delicious" apples by regulating organic acids metabolism and GABA shunt.


Assuntos
Malus , Acetatos , Aconitato Hidratase/metabolismo , Ciclopentanos , Frutas/metabolismo , Malus/metabolismo , NADP/metabolismo , Oxilipinas , Ácido gama-Aminobutírico
12.
J Agric Food Chem ; 70(18): 5658-5667, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35499968

RESUMO

The efficacy of trehalose on the lesion diameter of apples (cv. Golden Delicious) inoculated with Penicillium expansum was evaluated to screen the optimal concentration. The changes in gene expression and activity of the enzyme in starch, sorbitol, and energy metabolism were also investigated in apples after trehalose treatment. The results revealed that trehalose dipping reduced the lesion diameter of apples inoculated with P. expansum. Trehalose suppressed the activities and gene expressions of ß-amylase, NAD-sorbitol dehydrogenase, and NADP-sorbitol dehydrogenase, whereas it decreased the sorbitol 6-phosphate dehydrogenase gene expression and amylose, amylopectin, total starch, and reducing sugar contents. Additionally, trehalose improved the gene expressions and activities of α-amylase, starch-branching enzymes, total amylase, H+-ATPase, and Ca2+-ATPase, as well as soluble sugar, adenosine triphosphate, and adenosine diphosphate contents and energy charge in apples. These findings imply that trehalose could induce tolerance to the blue mold of apple fruit by regulating starch, sorbitol, and energy metabolism.


Assuntos
Anacardiaceae , Malus , Penicillium , Metabolismo Energético , Frutas/metabolismo , L-Iditol 2-Desidrogenase/metabolismo , Malus/metabolismo , Penicillium/metabolismo , Sorbitol , Amido/metabolismo , Açúcares/metabolismo , Trealose/metabolismo , Trealose/farmacologia
13.
Exp Anim ; 71(3): 376-384, 2022 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-35400714

RESUMO

Diabetic retinopathy (DR) is the prevalent microvascular complication of diabetes mellitus (DM), and it may lead to permanent blindness. The previous publication has indicated that both inflammatory response and oxidative stress are critical factors involved in DR progression, however, the accurate regulatory mechanism remains to be revealed. Src homology region 2 (SH2)-containing protein tyrosine phosphatase 2 (SHP2), a member of the protein tyrosine phosphatase family, was reported to play a role in diabetic nephropathy, whereas its function in DR was unknown and required further exploration. The level of phosphorylated, not the total, SHP2 increased in the retinas of rats with streptozotocin injection-induced DM. Further, the intravitreal injection of SHP2 shRNA lentivirus alleviated retinal pathological changes, and inhibited inflammatory response and oxidative stress, which were accompanied with Yes-associated protein 1 (YAP1) deactivation in DR rats. Additional co-immunoprecipitation results confirmed the interaction of SHP2 and YAP1. Collectively, our data preliminarily show that DR amelioration-induced by SHP2 inhibition in rats may attribute to the deactivation of YAP1 pathway.


Assuntos
Diabetes Mellitus , Retinopatia Diabética , Estresse Oxidativo , Proteína Tirosina Fosfatase não Receptora Tipo 11 , Proteínas de Sinalização YAP , Animais , Retinopatia Diabética/genética , Inativação Gênica , Proteína Tirosina Fosfatase não Receptora Tipo 11/genética , Proteína Tirosina Fosfatase não Receptora Tipo 11/metabolismo , Ratos , Transdução de Sinais , Proteínas de Sinalização YAP/genética , Proteínas de Sinalização YAP/metabolismo
14.
Biochem Biophys Res Commun ; 601: 101-108, 2022 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-35240496

RESUMO

A shielded geomagnetic field, also called the hypomagnetic field (HMF), interferes with the metabolic processes of various cells and animals exhibiting diverse effects in different models, however, its underlying mechanism remains largely unknown. In this study, we assessed the effect on the energy metabolism of SH-SY5Y cells in HMF and found that HMF-induced cell proliferation depends on glucose supply. HMF promoted SH-SY5Y cell proliferation by increasing glucose consumption rate via up-regulating anaerobic glycolysis in the cells. Increased activity of LDH, a key member of glycolysis, was possibly a direct response to HMF-induced cell proliferation. Thus, we unveiled a novel subcellular mechanism underlying the HMF-induced cellular response: the up-regulation of anaerobic glycolysis and repression of oxidative stress shifted cellular metabolism more towards the Warburg effect commonly observed in cancer metabolism. We suggest that cellular metabolic profiles of various cell types may determine HMF-induced cellular effects, and a magnetic field can be applied as a non-invasive regulator of cell metabolism.


Assuntos
Glucose , Neuroblastoma , Anaerobiose , Animais , Linhagem Celular Tumoral , Glicólise , Humanos , Campos Magnéticos , Neuroblastoma/metabolismo
15.
Plant Physiol Biochem ; 174: 43-50, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-35123260

RESUMO

Senescence is a pivotal factor that causes quality breakdown and economic loss of fruit after harvest. In this study, 'Golden Delicious' apples were used as the materials to investigate the effect of melatonin dipping on quality parameters and sucrose metabolism during room temperature storage. Postharvest melatonin treatment inhibited respiratory intensity and ethylene release, increased flesh firmness, soluble sugar, ascorbic acid, and soluble solid contents, and titratable acid in apples. Furthermore, melatonin treatment inhibited acid invertase and neutral invertase activities, increased sucrose synthase and sucrose phosphate synthase activities, and repressed the activities of sorbitol dehydrogenase, sorbitol oxidase and sucrose synthase cleavage in apple fruit. All these findings suggest that exogenous application of melatonin could maintain quality of 'Golden Delicious' apples by mediating the enzyme activity in sucrose metabolism.


Assuntos
Malus , Melatonina , Metabolismo dos Carboidratos , Frutas/metabolismo , Malus/metabolismo , Melatonina/metabolismo , Melatonina/farmacologia , Sacarose/metabolismo
16.
Zhen Ci Yan Jiu ; 46(8): 679-83, 2021 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-34472753

RESUMO

OBJECTIVE: To observe the effect of electroacupuncture (EA) at "Zhiyang" (GV9) and "Jizhong" (GV6) of the Governor Vessel on the expression of calcitonin gene related peptide (CGRP)and NOD-like receptor pyrin containing 3 (NLRP3) in the injured anterior horn (AH) of rats with spinal cord injury (SCI), so as to explore its mechanisms underlying improvement of SCI. METHODS: Thirty-six male SD rats were randomly divided into sham operation (sham) group, model group and EA group which were further randomly allocated to 7 day (d) and 14 d subgroups (n=6 per subgroup). The SCI model was established by clamping the exposed spinal cord with an aneurysm clip. Rats of the EA group received EA of GV6 and GV9 for 30 min, once daily for 7 and 14 days, respectively. The Basso, Beattie and Bresnahan (BBB) was used to assess changes of the locomotor function on the 1st, 3rd, 7th and 14th day after SCI. The protein expression levels of CGRP, NLRP3, apoptosis-associated speck-like protein (ASC) and cysteinyl aspartate specific protease-1 (caspase-1) in the anterior horn region of the spinal cord on the 7th and 14th after SCI were detected by Western blot. RESULTS: The BBB scores on the 1st, 3rd, 7th and 14th d after SCI were significantly lower in the model group than in the sham group (P<0.05), whereas the expression levels of NLRP3, ASC and caspase-1 proteins on day 7 and 14 were markedly higher in the model group than in the sham group (P<0.05, P<0.01). Compared with the model group, the BBB score and CGRP expression on the 7th and 14th d were significantly increased in the EA group (P<0.05,P<0.01), while the expression levels of NLRP3, ASC and caspase-1 were obviously decreased in the EA group (P<0.01). CONCLUSION: EA of GV6 and GV9 can improve the locomotion of SCI rats, which may be associated with its function in up-regulating the expression of CGRP and down-regulating the expression of NLRP3, ASC and caspase-1 proteins in the spinal anterior horn tissue.


Assuntos
Eletroacupuntura , Traumatismos da Medula Espinal , Animais , Calcitonina , Peptídeo Relacionado com Gene de Calcitonina/genética , Masculino , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , Ratos , Ratos Sprague-Dawley , Medula Espinal , Traumatismos da Medula Espinal/genética , Traumatismos da Medula Espinal/terapia
17.
Clin Chim Acta ; 522: 77-82, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34411556

RESUMO

BACKGROUND: Hypoxia-inducible factor 1alpha (HIF-1α) is implicated in the cell's response to hypoxia. We investigated whether serum HIF-1α concentrations are correlated with the severity and clinical outcome of severe traumatic brain injury (sTBI). METHODS: Serum HIF-1α concentrations were quantified in 104 sTBI patients and 80 healthy controls. Trauma severity was assessed using Glasgow coma scale (GCS). Glasgow outcome scale (GOS) score of 1-3 at post-trauma 90 days was defined as a poor outcome. Multivariate analyses were performed to discern the relationship between serum HIF-1α concentrations and outcome. RESULTS: Patients displayed significantly higher serum HIF-1α concentrations than controls (median, 294.9 versus 102.7 pg/ml). HIF-1α concentrations were intimately related to GCS scores (r = -0.62) and GOS scores (r = -0.64). 48 patients (46.2%) experienced a poor outcome. Serum HIF-1α concentrations > 280.2 pg/ml significantly distinguished patients with the development of poor outcome with 77.1% sensitivity and 69.6% specificity (AUC, 0.750; 95% CI: 0.655-0.829). Serum HIF-1α concentrations > 280.2 pg/ml emerged as an independent predictor for poor outcome (OR: 4.179; 95% CI: 1.024-17.052). CONCLUSIONS: Serum HIF-1α concentrations are tightly associated with trauma severity and poor 90-day outcome, substantializing serum HIF-1α as a promising prognostic biomarker for sTBI.


Assuntos
Lesões Encefálicas Traumáticas , Lesões Encefálicas Traumáticas/diagnóstico , Escala de Coma de Glasgow , Humanos , Hipóxia , Subunidade alfa do Fator 1 Induzível por Hipóxia , Prognóstico
18.
J Agric Food Chem ; 69(36): 10581-10591, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34432461

RESUMO

Intestinal stem cells (ISCs) are essential to maintain intestinal epithelial regeneration and barrier function. Our previous work showed that glucomannan from Aloe vera gel (AGP) alleviated epithelial damage, but the mechanism was still elusive. Herein, RNA-sequencing analysis showed that proliferation and differentiation of intestinal epithelial cells as well as the canonical Wnt pathway were involved in this process. Further experiments exhibited that AGP promoted nuclear translocation of ß-catenin and expression of transcription factor 7, increased the number of Lgr5+ ISCs, and differentiated epithelial cells in mice colon. Intriguingly, AGP reversed the inhibition of IEC-6 cells proliferation induced by an inhibitor of the canonical Wnt pathway. Hence, this study implied that AGP promoted proliferation and differentiation of colon stem cells via Wnt/ß-catenin signaling, which subsequently facilitated the regeneration of epithelial cells and alleviated colitis in mice. It may provide new insights into the role of polysaccharides in regulating intestinal homeostasis and relieving intestinal injury.


Assuntos
Via de Sinalização Wnt , beta Catenina , Animais , Diferenciação Celular , Proliferação de Células , Mucosa Intestinal/metabolismo , Mananas , Camundongos , Preparações de Plantas , Regeneração , Células-Tronco/metabolismo , beta Catenina/genética , beta Catenina/metabolismo
19.
Environ Sci Pollut Res Int ; 28(28): 38406-38425, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33728611

RESUMO

Increasing urban heat island and global warming have aroused serious thermal environmental problems and even harm people's thermal health. Because of the importance in people's daily life, a commercial pedestrianized block represents a symbol of a city or metropolis; therefore, focusing the attention on the thermal environment in such regions is very necessary. Most of the researches on the urban thermal environment are calculated by remote sensing data; limited by the low spatial resolution of remote sensing image, it may not obviously reflect the true thermal environment of the research site, especially in some microscale regions. Based on this, the new software ENVI-met is developed to research the thermal environment and forecast people's thermal sensation in a microscale region. Therefore, the objective of this study aims at conducting field measurement and numerical simulation to assess the thermal environment of a typical commercial pedestrianized space in southern China and assess the different urban design parameters in ameliorating the urban heat island effect. Our final results demonstrate a quantitative evidence for establishing a comprehensive standard for improving the thermal environment in a microscale region, and this study also can be a supplementary in the research field about improving thermal health.


Assuntos
Temperatura Alta , Sensação Térmica , China , Cidades , Aquecimento Global , Humanos
20.
J Agric Food Chem ; 69(2): 686-697, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33369397

RESUMO

Targeting autophagy and lysosome may serve as a promising strategy for cancer therapy. Tea polysaccharide (TP) has shown promising antitumor effects. However, its mechanism remains elusive. Here, TP was found to have a significant inhibitory effect on the proliferation of colon cancer line HCT116 cells. RNA-seq analysis showed that TP upregulated autophagy and lysosome signal pathways, which was further confirmed through experiments. Immunofluorescence experiments indicated that TP activated transcription factor EB (TFEB), a key nuclear transcription factor modulating autophagy and lysosome biogenesis. In addition, TP inhibited the activity of mTOR, while it increased the expression of Lamp1. Furthermore, TP ameliorated the lysosomal damage and autophagy flux barrier caused by Baf A1 (lysosome inhibitor). Hence, our data suggested that TP repressed the proliferation of HCT116 cells by targeting lysosome to induce cytotoxic autophagy, which might be achieved through mTOR-TFEB signaling. In summary, TP may be used as a potential drug to overcome colon cancer.


Assuntos
Autofagia/efeitos dos fármacos , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/metabolismo , Camellia sinensis/química , Neoplasias do Colo/fisiopatologia , Lisossomos/metabolismo , Extratos Vegetais/farmacologia , Polissacarídeos/farmacologia , Serina-Treonina Quinases TOR/metabolismo , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/genética , Morte Celular/efeitos dos fármacos , Neoplasias do Colo/tratamento farmacológico , Neoplasias do Colo/genética , Neoplasias do Colo/metabolismo , Células HCT116 , Humanos , Transdução de Sinais/efeitos dos fármacos , Serina-Treonina Quinases TOR/genética
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