Evaluation of right ventricle echocardiographic variables in apparently healthy domestic shorthair cats.

Right ventricular structural and functional changes result from many cardiovascular disorders in cats. Accordingly, echocardiographic evaluation of the size and function of the right ventricle (RV) provides important prognostic information in many conditions affecting the right heart. Therefore, detection of these changes is clinically important and needs reference values to ease the diagnosis. The present study was designed to calculate structural and functional right ventricular variables in 10 apparently healthy cats (six males and four females, average age 1 year old and body weight 2.70 - 4.80 kg) with no sedation. For this purpose, The minimum, maximum, mean ± standard error of the mean of right ventricle internal dimension in systole and diastole (cm), right ventricle free wall thickness in systole and diastole (cm), fractional shortening (%), right ventricle volume in systole and diastole (mL), right ventricle area in systole and diastole (cm2), ejection fraction (%) and fractional area change (%) were measured and reported. Descriptive statistics were provided for all calculated variables. Statistical correlation of the collected variables with body weight, gender and heart rate were analyzed. Results showed that there is a significant correlation between heart rate with body weight and between systolic and diastolic RV volumes with gender. The results of the present study would help clinicians in the diagnosis of the right heart changes in DSH cats.

In silico analysis of sporozoite surface antigen 1 of Theileria annulata (TaSPAG1) for multi-epitope vaccine design against theileriosis.

Tropical theileriosis is a protozoan infection caused by Theileria annulata, which significantly affects cattle worldwide. This study was aimed to analyze the TaSPAG1 protein and design a novel multi-epitope vaccine candidate. Online tools were employed for the prediction of Physico-chemical properties, antigenicity, allergenicity, solubility, transmembrane domains and signal peptide, posttranslational modification (PTM) sites, secondary and tertiary structures as well as intrinsically disordered regions, followed by identification and screening of potential linear and conformational B-cell epitopes and those peptides having affinity to bind bovine major histocompatibility complex class I (MHC-I) molecules. Next, a multi-epitope vaccine construct was designed and analyzed. This 907-residue protein was hydrophilic (GRAVY: -0.399) and acidic (pI: 5.04) in nature, with high thermotolerance (aliphatic: 71.27). Also, 5 linear and 12 conformational B-cell epitopes along with 8 CTL epitopes were predicted for TaSPAG1. The 355-residue vaccine candidate had a MW of about 35 kDa and it was antigenic, non-allergenic, soluble and stable, which was successfully interacted with cattle MHC-I molecule and finally cloned into the pET28a(+) vector. Further wet studies are required to assess the vaccine efficacy in cattle. Supplementary Information: The online version contains supplementary material available at 10.1007/s40203-023-00153-5.

Echocardiographic assessment of right ventricular blood flow and tricuspid annular plane systolic excursion in apparently healthy domestic short-haired cats.

Right ventricular parameters change in many cardiovascular diseases; so, the presence of normal right ventricular parameters is necessary to diagnose these diseases. Ten clinically healthy adult domestic short-haired cats including six males and four females in the range of 2.70 to 4.80 kg were studied using echocardiography without sedation. The speed and pressure of blood flow through the tricuspid and pulmonary valves, speed of the movement of tricuspid valve and tricuspid annular plane systolic excursion (TAPSE) were recorded using conventional pulsed-wave (PW) Doppler, tissue Doppler imaging (TDI) and M-mode, respectively. No significant statistical differences were observed between the measured values and sex, heart rate and body weight. A positive correlation was observed between the maximum velocity of the right ventricular outflow tract and heart rate and also between TAPSE slope and body weight. With determining the normal values of PW-TDI of the right ventricle in apparently healthy domestic short-haired cats, it is expected that with providing normal reference values, the diagnosis of heart diseases especially asymptomatic heart disease be achieved as soon as possible to provide the best appropriate therapeutic management and monitoring decision about them.

In silico design of a novel peptide-based vaccine against the ubiquitous apicomplexan Toxoplasma gondii using surface antigens.

Human toxoplasmosis is a global public health concern and a commercial vaccine is still lacking. The present in silico study was done to design a novel vaccine candidate using tachyzoite-specific SAG1-realted sequence (SRS) proteins. Overlapping B-cell and strictly-chosen human MHC-I binding epitopes were predicted and connected together using appropriate spacers. Moreover, a TLR4 agonist, human high mobility group box protein 1 (HMGB1), and His-tag were added to the N- and C-terminus of the vaccine sequence. The final vaccine had 442 residues and a molecular weight of 47.71 kDa. Physico-chemical evaluation showed a soluble, highly antigenic and non-allergen protein, with coils and helices as secondary structures. The vaccine 3D model was predicted by ITASSER server, subsequently refined and was shown to possess significant interactions with human TLR4. As well, potent stimulation of cellular and humoral immunity was demonstrated upon chimeric vaccine injection. Finally, the outputs showed that this vaccine model possesses top antigenicity, which could provoke significant cell-mediated immune profile including IFN-γ, and can be utilized towards prophylactic purposes. Supplementary Information: The online version contains supplementary material available at 10.1007/s40203-023-00140-w.

Computational Clues of Immunogenic Hotspots in Plasmodium falciparum Erythrocytic Stage Vaccine Candidate Antigens: In Silico Approach.

Malaria is the most pernicious parasitic infection, and Plasmodium falciparum is the most virulent species with substantial morbidity and mortality worldwide. The present in silico investigation was performed to reveal the biophysical characteristics and immunogenic epitopes of the 14 blood-stage proteins of the P. falciparum using comprehensive immunoinformatics approaches. For this aim, various web servers were employed to predict subcellular localization, antigenicity, allergenicity, solubility, physicochemical properties, posttranslational modification sites (PTMs), the presence of signal peptide, and transmembrane domains. Moreover, structural analysis for secondary and 3D model predictions were performed for all and stable proteins, respectively. Finally, human helper T lymphocyte (HTL) epitopes were predicted using HLA reference set of IEDB server and screened in terms of antigenicity, allergenicity, and IFN-γ induction as well as population coverage. Also, a multiserver B-cell epitope prediction was done with subsequent screening for antigenicity, allergenicity, and solubility. Altogether, these proteins showed appropriate antigenicity, abundant PTMs, and many B-cell and HTL epitopes, which could be directed for future vaccination studies in the context of multiepitope vaccine design.