Microbial risk assessment of dairy products from retail marketplaces in Basrah province, Iraq.

Background: Milk-borne bacteria cause degradation of milk products and constitute a significant risk to public health. Aim: The objectives of the present study are to determine the microbiological quality of dairy products and to investigate pathogenic microorganisms. Methods: A total of 60 samples of raw milk, homemade cheese, and yogurt were randomly selected from different retail marketplaces in Basrah. The bacteriological and biochemical tests were utilized to identify the pathogens in dairy samples, as well as the molecular technique was used as an accurate diagnostic test. Results: The prevalence of contamination of milk products with various isolates was estimated as 50% (95% Cl: 36.8-63.2). The mean of total bacteria count for cheese was 7.29 ± 2.70, raw milk 4.62 ± 2.86, and yogurt 2.87 ± 1.05, with a significant p-value (p = 0.001). The mean count of aerobic spore-forming (ASF) contaminated raw milk was analyzed as 3.77 ± 1.18 and less contamination detected in the yogurt samples with mean of ASF was estimated as 2.52 ± 1.47 SD log 10 CFU/ml. A range of important microorganisms to human health were identified by employing the VITEK_2 system and sequencing 16S rDNA gene, including Staphylococcus aureus, Escherichia coli, Pseudomonas aerogenosa, and Bacillus cereus. Conclusion: The study indicates that there is a high level of bacterial contamination in dairy products with different bacteria species, which is medically important. Therefore, food safety management must be implemented to reduce biological risks carried by dairy products and ensure healthy food for consumers.

Antimicrobial efficacy of Thymus vulgaris extract against some Staphylococcus species isolated from subclinical mastitis in cattle in Basrah province, Iraq.

Background: Thymus vulgaris extracts can play a significant role as alternatives for antimicrobial agents against bovine staphylococcus mastitis. Aim: This research's goal was to evaluate the antibacterial properties of an extract from T. vulgaris as an alternative to antibiotics for bovine Staphylococcus mastitis. In addition, it is important to know the effect of the extraction methods (hot alcoholic, cold alcoholic, and hot water extract) on their effectiveness. Methods: Two hundred ten cow milk samples from different areas of Basrah province had been suffering subclinical mastitis reported by using the California mastitis test (CMT). Staphylococcus species were identified by conventional microbiological technique, GP24 Kit, and nuc gene. Antimicrobial activity of various concentrations of T. vulgaris extracted (75, 50, 25) mg/ml with different methods of extraction (hot alcoholic, cold alcoholic, and hot water extract). Results: Out of 210 samples, 99 (47.1%) were positive for the CMT, and the identification rate of Staphylococci spp. by conventional microbiological technique and GP24 kit was 78 (78.8%). Out of 78 isolates of Staphylococcus spp. 48 (61.5%) were identified as Staphylococcus aureus, by using both molecular techniques using PCR and miniaturized Kit GP24 and employing the miniature GP24, the remaining 30 (38.5%) were determined to be different species of Staphylococcus. Antibacterial activity of various concentrations of T. vulgaris extracted (75, 50, 25) mg/ml with different methods of extraction revealed that hot alcoholic extract (100%) was more effective than cold alcoholic extract (66.7%), whereas there is no effect on the bacteria species with the hot water extract. Conclusion: Thymus vulgaris extracts can play a significant role as alternatives for antimicrobial agents against bovine staphylococcus mastitis.