Effect of TX-100 pretreatment on carbon paste electrode for selective sensing of dopamine in presence of paracetamol.

Dopamine (DA) is one of the chief neurotransmitters present in the central nervous system of mammals. Therefore detection of DA in presence of various analytes like paracetamol has great importance. In the current work, we are proposing that Triton X-100 (TX-100) pretreated carbon paste electrode (CPE) can be useful to detect the DA selectively in presence of PA. After the pretreatment CPE can detect DA in presence of PA effectively. Cyclic voltammetry was employed to observe the amplified electron transfer reaction between the modified CPE and DA. To understand electron transfer regioselectivity at the TX-100 pretreated CPE, a dual descriptor was used. The prepared electrode showed satisfactory stability when kept under ambient conditions. The proposed approach also showed excellent analytical applicability to identify DA and PA in commercial formulations. The scope of the work is limited to detecting DA in presence of PA. We will consider the other interferes for future works.

Effect of graphite oxide and exfoliated graphite oxide as a modifier for the voltametric determination of dopamine in presence of uric acid and folic acid.

In the present work, exfoliated graphite oxide (E-GO) was prepared by sonicating graphite oxide (GO) (prepared by modified Hummer's and Offemam methods). Prepared GO and E-GO were characterized using infrared absorption spectroscopy, X-ray diffraction, and scanning electron microscopy. The electrocatalytic properties of GO and E-GO towards detection of dopamine (DA), uric acid (UA), and folic acid (FA) were investigated using cyclic voltammetry and differential pulse voltammetry. Our results revealed that E-GO has a slighter advantage over the GO as an electrode modifier for detection DA, UA, and FA, which might be ascribed to the good conductivity of E-GO when compared to the GO.

Infrared thermography: A potential noninvasive tool to monitor udder health status in dairy cows.

The animal husbandry and livestock sectors play a major role in the rural economy, especially for the small and marginal farmers. India has the largest livestock population in the world and ranks first in the milk production. Mastitis is the most common and expensive infectious disease in dairy cattle. The global economic losses per year due to mastitis amounts to USD 35 billion and for Indian dairy industry ₹6000 crores per year. Early detection of mastitis is very important to reduce the economic loss to the dairy farmers and dairy industry. Automated methods for early and reliable detection of mastitis are currently in focus under precision dairying. Skin surface temperature is an important indicator for the diagnosis of cow's illnesses and for the estimation of their physiological status. Infrared thermography (IRT) is a simple, effective, on-site, and noninvasive method that detects surface heat, which is emitted as infrared radiation and generates pictorial images without causing radiation exposure. In human and bovine medicine, IRT is used as a diagnostic tool for assessment of normal and physiological status.

Rumen-protected choline: A significance effect on dairy cattle nutrition.

Choline is a vitamin-like substance it has multi-function in animal production, reproduction, and health. The transition period is most crucial stage in lactation cycle of dairy cows due to its association with negative hormonal and energy balances. Unfortunately, unprotected choline easily degrades in the rumen; therefore, choline added to the diet in a rumen-protected form. The use of rumen-protected choline (RPC) is a preventive measurement for the fatty liver syndrome and ketosis; may improve milk production as well as milk composition and reproduction parameters. This review summarizes the effectiveness of RPC on animal production, health, and reproduction.

Investigation of body and udder skin surface temperature differentials as an early indicator of mastitis in Holstein Friesian crossbred cows using digital infrared thermography technique.

AIM: The objective of this study was to investigate the ability of infrared thermography (IRT) technique and its interrelationship with conventional mastitis indicators for the early detection of mastitis in Holstein Friesian (HF) crossbred cows. MATERIALS AND METHODS: A total of 76 quarters of lactating HF crossbred (Bos indicus × Bos taurus) cows (n=19) were monitored for body temperature (i.e., eye temperature) and udder skin surface temperature (USST) before milking using forward-looking infrared (FLIR) i5 camera. Milk samples were collected from each quarter and screened for mastitis using Somatic Cell Count (SCC), Electrical Conductivity (EC), and California mastitis test. Thermographic images were analyzed using FLIR Quick Report 1.2 image analysis software. Data on body and USST were compiled and analyzed statistically using SPSS 16.0 and Sigmaplot 11. RESULTS: The mean±standard deviation (SD) body (37.23±0.08°C) and USST (37.22±0.04°C) of non-mastitic cow did not differ significantly; however, the mean USST of the mastitis-affected quarters were significantly higher than the body temperature and USST of unaffected quarters (p<0.001). The mean±SD USST of the subclinical mastitis (SCM) and clinical mastitis-affected quarters were 38.08±0.17 °C and 38.25±0.33 °C, respectively, which is 0.72 and 1.05 °C higher than the USST temperature of unaffected quarters. The USST was positively correlated with EC (r=0.95) and SCC (r=0.93). The receiver operating characteristic curve analysis revealed a higher sensitivity for USST in early prediction of SCM with a cut-off value of >37.61°C. CONCLUSION: It is concluded that infrared thermal imaging technique could be used as a potential noninvasive, quick cow-side diagnostic technique for screening and early detection of SCM and clinical mastitis in crossbred cows.