RESUMO
Phytochemical investigation on the ethyl acetate fraction of the leaves of Epigynum cochinchinensis led to the isolation of a new C21 pregnane glycoside, epigycoside B (1), together with three known analogues. Their structures were elucidated on the basis of extensive spectroscopic techniques, including UV, MS, and NMR experiments, as well as the chemical methods. Compound 1 displayed in vitro immunosuppressive activity against concanavalin A (Con A)/Lipopolysaccharides (LPS)-stimulated proliferation of mice splenocyte. The activity was significant as compared with control group at 50 µM concentration.
Assuntos
Apocynaceae/química , Glicosídeos/farmacologia , Imunossupressores/isolamento & purificação , Pregnanos/farmacologia , Animais , Proliferação de Células/efeitos dos fármacos , Concanavalina A/efeitos dos fármacos , Glicosídeos/química , Glicosídeos/imunologia , Glicosídeos/isolamento & purificação , Imunossupressores/química , Imunossupressores/farmacologia , Lipopolissacarídeos , Camundongos , Estrutura Molecular , Folhas de Planta/química , Pregnanos/química , Pregnanos/imunologia , Pregnanos/isolamento & purificação , Análise Espectral , Baço/citologiaRESUMO
The title mol-ecule, C(13)H(18)N(2)O(3), contains a benzene ring fused to an oxazine ring and one tert-but-oxy-carbonyl group bound to the N atom of the oxazine ring. A weak intra-molecular C-Hâ¯O inter-action occurs. In the crystal, inter-molecular N-Hâ¯O and C-Hâ¯O hydrogen bonds stack the mol-ecules down the b axis. Weak C-Hâ¯N contacts connect the stacks, generating a three-dimensional network.
RESUMO
In the title compound, C(9)H(9)FO(3), the dihedral angle between the carboxyl group and the benzene ring is 79.4â (3)°. In the crystal, mol-ecules form centrosymmetric dimers through pairs of classical O-Hâ¯O hydrogen bonds. These are further linked by weaker C-Hâ¯O inter-actions, forming a three-dimensional network.
RESUMO
AIM: To study the inhibitory effect of 23-HBA on angiogenesis in vitro. METHODS: The effect of 23-hydroxy butulinic acid (23-HBA) on the in vitro proliferation of human microcapillary endothelial cells(HMECs) was examined by sulfonylrhodamine B (SRB) assay. The effect of 23-HBA on endothelial cell migration, and tubule formation on Matrigel was also observed. The CD31 expression in HMECs was dectected by immunohistochemical staining. RESULTS: The proliferation of HMECs was inhibited significantly by 23-HBA with IC(50) being 40.44 mg/L. 23-HBA inhibited endothelial cell migration and tubule formation in a dose-dependent manner. The expression of CD31 in HMECs was reduced after treatment with 10 mg/L 23-HBA. CONCLUSION: 23-HBA can inhibit angiogenesis in vitro, which would become a promising antiangiogenic drug.
