LncRNA TSLNC8 inhibits proliferation of breast cancer cell through the miR-214-3p/FOXP2 axis.

OBJECTIVE: In multiple cancers, heterozygosity is frequently lost for the tumor-suppressive long noncoding RNA (lncRNA). The expression, function, and molecular mechanisms of tumor suppressive lncRNA on chromosome 8p12 (TSLNC8) in breast cancer are still unknown. MATERIALS AND METHODS: QRT-PCR assays were carried out to evaluate the level of TSLNC8 in breast cancer tissues and cell lines. MTT, colony formation, and anchorage-independent growth assays were performed to investigate the effect of TSLNC8 on cell proliferation, and flow cytometry assays were conducted to detect cell percent of different phases. Luciferase reporter assays were used to confirm the interaction of different molecules. RESULTS: TSLNC8 is significantly increased in breast cancer tissues and cell lines. Up-regulation of TSLNC8 reduces the proliferation capacity of breast cancer cells and the transition from G1 to S phase of the cell cycle. Further analysis indicated that TSLNC8 could directly bind to miR-214-3p. Up-regulation of miR-214-3p may attenuate the suppressive role of TSLNC8 on the proliferation capacity of breast cancer cells. Moreover, miR-214-3p was found to directly interact with the 3'-untranslated region (UTR) of Forkhead box P2 (FOXP2) in luciferase assays, suggesting that FOXP2 may be one of the downstream targets of miR-412-3p. CONCLUSIONS: TSLNC8 was found to inhibit the proliferation and G1/S phase transition of breast cancer cells, an effect mediated by miR-214-3p/FOXP2 axis. Our study provides evidence that TSLNC8 may act as a suppressive lncRNA and represent a novel therapeutic target for breast cancer therapy.

Strain and mechanical behavior measurements of soft tissues with digital speckle method.

Soft tissues of the body are composite, typically being made up of collagen and elastin fibers with high water contents. The strain measurement in soft tissues has proven to be a difficult task. The digital speckle method, combined with the image processing technique, has many advantages such as full field, noncontact, and real time. We focus on the use of an improved digital speckle correlation method (DSCM) and time-sequence electric speckle pattern interferometry (TSESPI) to noninvasively obtain continual strain measurements on cartilage and vessel tissues. Monoaxial tensile experiments are well designed and performed under constant temperature and the necessary humidity with smart sensors. Mechanical behaviors such as the tensile modulus and Poisson ratio of specimens are extracted based on the deformation information. A comparison of the advantages and the disadvantages of these techniques as well as some problems concerning strain measurements in soft tissues are also discussed.

Buccal bone plate thickness of the Asian people.

Distances from the apex to the buccal bone plate were measured on the computed tomography (CT) images of 1806 teeth from 66 patients, using an image analyzer program (Image-Pro Plus, Ver. 4.0, Media Cybernetics). In the mandible, the mean distance from the distal apex of the mandibular second molar to the buccal bone plate was the largest distance measured, at 8.51 mm, followed by distance from the mesial root to the buccal bone (7.34 mm). In the mandibular first molar, the mean distal and mesial bone thicknesses were 5.18 mm and 4.09 mm, respectively. However, when there were two distal roots, the distance of the disto-lingual root to the buccal plate was found to be 9.52 mm, which constitutes the greatest measured thickness. In the maxillary buccal roots, the distances from the mesio-buccal and disto-buccal root of the second molar to the buccal bone plate were the largest, at 4.63 mm and 3.61 mm, respectively. The average distances from the palatal apex of the maxillary first and second molars to the buccal bone plate were 10.69 mm and 10.17 mm, respectively, while, from the palatal bone plate, average distances of 3.15 mm and 3.08 mm were measured. Special considerations, such as bony lid approach, lingual approach, or intentional replantation may be required, especially when a patient has a surgical need in the second molars and the disto-lingual root of the mandibular first molar, or in the palatal root of the maxillary molars.

[Applying photocclusion method to study occlusion mechanics on wedge shaped teeth defect with computer assisting].

OBJECTIVE: Study the occlusal force of wedge shaped teeth defect and its relation with the clinical condition. METHODS: Use the method of clinical examination and plaster model measurement to decide the parameters of axial gradient degree of the teeth, abrasion index, missing teeth number and so on; Adopt the computer image processing technology and program to analyse the photocclusion sheet, take the date and image information of occlusion force, areas, and occlusal stress of wedge shaped teeth defect. RESULTS: The occlusion contact data and graph of wedge-shaped teeth defect had the features of trauma occlusion. 42.97% of them has the larger axial gradient degree, 96.09% of them has abration in different classes, 46.29% missing the front teeth and 57.03% missing the buccal teeth. Occlusal contact force increase took up 78.91% in the wedge shaped teeth defect. CONCLUSION: It can be concluded that the not uniformly distributed occlusal force produced from the larger axial gradient degree, the defect of dentition, and the abrasion of teeth, may be the important factors which cause wedge shaped teeth defect.