Genomic analysis of wolves from Pakistan clarifies boundaries among three divergent wolf lineages.

Among the three main divergent lineages of gray wolf (Canis lupus), the Holarctic lineage is the most widespread and best-studied, particularly in North America and Europe. Less is known about Tibetan (also called Himalayan) and Indian wolf lineages in southern Asia, especially in areas surrounding Pakistan where all three lineages are thought to meet. Given the endangered status of the Indian wolf in neighboring India and unclear southwestern boundary of the Tibetan wolf range, we conducted mitochondrial and genome-wide sequencing of wolves from Pakistan and Kyrgyzstan. Sequences of the mitochondrial D-loop region of 81 wolves from Pakistan indicated contact zones between Holarctic and Indian lineages across the northern and western mountains of Pakistan. Reduced-representation genome sequencing of 8 wolves indicated an east-to-west cline of Indian to Holarctic ancestry, consistent with a contact zone between these two lineages in Pakistan. The western boundary of the Tibetan lineage corresponded to the Ladakh region of India's Himalayas with a narrow zone of admixture spanning this boundary from the Karakoram Mountains of northern Pakistan into Ladakh, India. Our results highlight the conservation significance of Pakistan's wolf populations, especially the remaining populations in Sindh and Southern Punjab that represent the highly endangered Indian lineage.

Ungulate spatiotemporal responses to contrasting predation risk from wolves and snow leopards.

Spatial responses to risk from multiple predators can precipitate emergent consequences for prey (i.e. multiple-predator effects, MPEs) and mediate indirect interactions between predators. How prey navigate risk from multiple predators may therefore have important ramifications for understanding the propagation of predation-risk effects (PREs) through ecosystems. The interaction of predator and prey traits has emerged as a potentially key driver of antipredator behaviour but remains underexplored in large vertebrate systems, particularly where sympatric prey share multiple predators. We sought to better generalize our understanding of how predators influence their ecosystems by considering how multiple sources of contingency drive prey distribution in a multi-predator-multi-prey system. Specifically, we explored how two sympatric ungulates with different escape tactics-vertically agile, scrambling ibex Capra sibirica and sprinting argali Ovis ammon-responded to predation risk from shared predators with contrasting hunting modes-cursorial wolves Canis lupus and vertical-ambushing, stalking snow leopards Panthera uncia. Contrasting risk posed by the two predators presented prey with clear trade-offs. Ibex selected for greater exposure to chronic long-term risk from snow leopards, and argali for wolves, in a nearly symmetrical manner that was predictable based on the compatibility of their respective traits. Yet, acute short-term risk from the same predator upended these long-term strategies, increasing each ungulates' exposure to risk from the alternate predator in a manner consistent with a scenario in which conflicting antipredator behaviours precipitate risk-enhancing MPEs and mediate predator facilitation. By contrast, reactive responses to wolves led ibex to reduce their exposure to risk from both predators-a risk-reducing MPE. Evidence of a similar reactive risk-reducing effect for argali vis-à-vis snow leopards was lacking. Our results suggest that prey spatial responses and any resulting MPEs and prey-mediated interactions between predators are contingent on the interplay of hunting mode and escape tactics. Further investigation of interactions among various drivers of contingency in PREs will contribute to a more comprehensive understanding and improved forecasting of the ecological effects of predators.

Guidelines for Telemetry Studies on Snow Leopards.

Animal-borne tracking devices have generated a wealth of new knowledge, allowing us to better understand, manage and conserve species. Fitting such tracking devices requires that animals are captured and often chemically immobilized. Such procedures cause stress and involve the risk of injuries and loss of life even in healthy individuals. For telemetry studies to be justifiable, it is vital that capture operations are planned and executed in an efficient and ethical way. Project objectives must be clearly articulated to address well-defined knowledge gaps, and studies designed to maximize the probability of achieving those goals. We provide guidelines for how to plan, design, and implement telemetry studies with a special emphasis on snow leopards that are typically captured using foot snares. We also describe the necessary steps to ensure that captures are conducted safely, and with minimal stress to animals.

Responses of snow leopards, wolves and wild ungulates to livestock grazing in the Zorkul Strictly Protected Area, Tajikistan.

Long recognized as a threat to wildlife, livestock grazing in protected areas has the potential to undermine conservation goals, via competition, habitat degradation, human-carnivore conflict and disruption of predator-prey relationships. In the Strictly Protected Area Zorkul in Tajikistan (Zorkul Reserve), grazing is commonplace despite official prohibition, with potentially detrimental effects on local fauna, in particular, snow leopard Panthera uncia, wolf Canis lupus, brown bear Ursus arctos, argali sheep Ovis ammon, Asiatic ibex Capra sibirica, and long-tailed marmot Marmota caudata. To understand the impacts of grazing and associated human pastoralism on the large mammal community in Zorkul Reserve we used data from camera traps to build models of ungulate and carnivore site use intensity, and we investigated carnivore summer diets using microscopic scat analysis. While sample sizes limited our inference for several species, we found that site use of the most common ungulate, argali, decreased with proximity to herder's camps, indicating possible displacement into sub-optimal habitats. However, no such pattern was present in carnivore site use. For wolf and snow leopard, the most frequently encountered prey items were argali and marmot, while bear depended almost exclusively on marmot. While current pastoralist practices in the reserve may not be incompatible with wildlife presence, our findings suggest that pastoralism may negatively impact ungulates by displacing them from otherwise suitable habitats, with unknown fitness consequences for ungulates or the predators that depend upon them. Managing Zorkul Reserve and other actively grazed protected areas to meet potentially competing demands of local pastoralist communities and conservation will require careful consideration of such interactions to minimize the risk of cascading negative impacts on wildlife.