Salivary Macrophage Inflammatory Protein-1α Levels in Periodontitis Subjects Receiving Non-surgical Periodontal Therapy With and Without Photo-Biomodulation: A Prospective Interventional Controlled Trial.

Introduction Periodontitis is a complex interplay of bacterial infection and host response. Non-surgical periodontal therapy (NSPT) is effective but limited in thoroughly debriding challenging areas, often leading to recurrent bacterial colonization. Photobiomodulation (PBM), involving precise doses of laser photonic energy, has shown potential in modulating inflammation and promoting healing. This study investigates the impact of NSPT alone and NSPT combined with PBM on clinical parameters and salivary macrophage inflammatory protein-1α (MIP-1α) levels in periodontitis patients. Methods Ethical approval was obtained, and informed consent was secured from all participants. Sixty periodontitis patients aged 30-60 were selected and divided into two groups: Group 1 (NSPT alone) and Group 2 (NSPT with PBM). Clinical parameters including the plaque index (PI), gingival index (GI), probing pocket depth (PPD), and clinical attachment level (CAL) were assessed at baseline and 21 days post-treatment. Salivary MIP-1α levels were measured using an enzyme-linked immunosorbent assay (ELISA). Results Both groups showed significant improvements in the PI, GI, PPD, and CAL from baseline to 21 days post-treatment. Group 2 exhibited greater reductions in the GI, PPD, and CAL compared to Group 1. Salivary MIP-1α levels post-intervention showed reduction in both groups but were not statistically significant between the groups. Conclusion NSPT combined with PBM demonstrates greater clinical improvements than NSPT alone, highlighting PBM as an effective adjunctive therapy. Both groups showed a reduction in MIP-1α levels post-intervention, suggesting the potential of MIP-1α as a biomarker for periodontal disease activity.

Assessment of Knowledge and Awareness of Ultrasonic Bone Surgery Among Periodontology and Oral Surgery Postgraduate Students in Maharashtra.

Introduction Ultrasonic bone surgery, or piezosurgery, is a novel technique utilizing ultrasonic vibrations to cut bone while minimizing damage to adjacent soft tissues. This study aims to evaluate the knowledge and awareness of ultrasonic bone surgery among periodontology and oral surgery postgraduate students in Maharashtra, India. Methods A cross-sectional, questionnaire-based study was conducted among 180 postgraduate students (84 in periodontology and 96 in oral surgery). A Google Form questionnaire (Google LLC, Mountain View, CA, United States) with 15 close-ended questions was distributed via email. Data were analyzed using SPSS Statistics version 25 (IBM Corp. Released 2017. IBM SPSS Statistics for Windows, Version 25.0. Armonk, NY: IBM Corp.) employing frequency percentages and Pearson Chi-square tests for intergroup comparisons. Results Of the participants, 80 (95.3%) of periodontology and 91 (94.7%) of oral surgery postgraduates were aware of ultrasonic bone surgery. Significant differences were found in the knowledge of critical bone damage temperature (p=0.009) and postoperative complications (p=0.037), with periodontology postgraduates showing higher awareness. Both groups recognized ultrasonic bone surgery's precision, safety, and reduced trauma advantages. Overall knowledge and awareness were higher among periodontology postgraduates (n=84, 87.5%) than oral surgery postgraduates (n=96, 78.3%). Conclusion Both groups demonstrated a good understanding of ultrasonic bone surgery, with periodontology postgraduates showing notably higher awareness. These findings underscore the importance of integrating ultrasonic bone surgical techniques into dental education to enhance patient care and keep pace with technological advancements. Further studies with larger sample sizes across India are recommended.

Comparative Evaluation of Salivary Cathelicidin and 8-Isoprostane Levels Among Smokeless Tobacco Users and Non-users and Their Correlation With Periodontal Health and Disease: A Cross-Sectional Study.

Introduction Periodontal diseases arise from host-microbial interactions influenced by tobacco products. Salivary antimicrobial peptides such as salivary cathelicidin and prostaglandins such as 8-isoprostane are part of the inflammatory cascade affecting periodontal disease pathogenesis. Methodology A total of 93 patients, 31 in each group that is healthy, periodontitis, and periodontitis with smokeless tobacco habit patients, were enrolled. The case history was recorded, and clinical examination was performed using periodontal parameter analysis of oral hygiene index simplified (OHIS), Russell's index, periodontal pocket depth (PPD), and clinical attachment level (CAL). The saliva samples were collected and subjected to an enzyme-linked immunosorbent assay (ELISA) to evaluate cathelicidin and 8-isoprostane. The results were analysed and compared statistically. Results The OHIS, Russell's index, pocket probing depth, and CAL were high in patients with periodontitis and tobacco habit (p<0.001). The cathelicidin levels were the highest in patients with periodontitis and the tobacco habit (1.6 g/mL). The level of 8-isoprostane was the highest in patients with periodontitis with tobacco habit (1.8 pg/mL). Smokeless tobacco users showed higher levels of cathelicidin and 8-isoprostane in periodontitis with tobacco than in the healthy group. Conclusion Increased cathelicidin and 8-isoprostane levels in smokeless tobacco users with periodontitis suggest risk biomarkers for tobacco-influenced periodontitis.

Knowledge, Attitudes, and Practices Regarding Periodontal Diseases Among First-Year Undergraduates of Different Faculties of Krishna Vishwa Vidyapeeth University.

Introduction Periodontal diseases, encompassing gingivitis and periodontitis, are prevalent and complex conditions with significant implications for overall health, particularly in India. This study aimed to assess the knowledge, attitudes, and practices (KAP) regarding periodontal diseases among first-year undergraduate students across various faculties at Krishna Vishwa Vidyapeeth Deemed to be University, Karad. Methods A cross-sectional questionnaire-based survey was conducted among 200 students from the medical, dental, physiotherapy, pharmacy, and nursing faculties. Descriptive statistics and Pearson's chi-squared test were utilized for data analysis. Results They revealed suboptimal oral hygiene practices among participants, with only 30% reporting twice-daily tooth cleaning. Additionally, less than half of the participants used soft toothbrushes and fluoridated toothpaste. Notably, 40% had never visited a dentist. While 86% recognized the correlation between gum and systemic health, significant proportions had experienced symptoms of gum disease. Dental first-year undergraduate students have a better understanding, attitude, and practices toward periodontal diseases, while nursing first-year undergraduate students displayed the lowest KAP scores compared to other faculties. Conclusion The first-year dental students demonstrated a better understanding, attitude, and behavior towards periodontal diseases compared to students in other faculties. Our study emphasizes the necessity of incorporating oral and periodontal health education into both healthcare professional curricula and those of higher secondary school students. Such educational programs will empower individuals to enhance their oral and systemic health. Moreover, equipping medical and paramedical practitioners with proper knowledge of periodontal health will ultimately lead to improved oral health outcomes for the general population.