RESUMO
Three young northern temperate forest communities in the north-central United States were exposed to factorial combinations of elevated carbon dioxide (CO2 ) and tropospheric ozone (O3 ) for 11 years. Here, we report results from an extensive sampling of plant biomass and soil conducted at the conclusion of the experiment that enabled us to estimate ecosystem carbon (C) content and cumulative net primary productivity (NPP). Elevated CO2 enhanced ecosystem C content by 11%, whereas elevated O3 decreased ecosystem C content by 9%. There was little variation in treatment effects on C content across communities and no meaningful interactions between CO2 and O3 . Treatment effects on ecosystem C content resulted primarily from changes in the near-surface mineral soil and tree C, particularly differences in woody tissues. Excluding the mineral soil, cumulative NPP was a strong predictor of ecosystem C content (r(2) = 0.96). Elevated CO2 enhanced cumulative NPP by 39%, a consequence of a 28% increase in canopy nitrogen (N) content (g N m(-2) ) and a 28% increase in N productivity (NPP/canopy N). In contrast, elevated O3 lowered NPP by 10% because of a 21% decrease in canopy N, but did not impact N productivity. Consequently, as the marginal impact of canopy N on NPP (∆NPP/∆N) decreased through time with further canopy development, the O3 effect on NPP dissipated. Within the mineral soil, there was less C in the top 0.1 m of soil under elevated O3 and less soil C from 0.1 to 0.2 m in depth under elevated CO2 . Overall, these results suggest that elevated CO2 may create a sustained increase in NPP, whereas the long-term effect of elevated O3 on NPP will be smaller than expected. However, changes in soil C are not well-understood and limit our ability to predict changes in ecosystem C content.
Assuntos
Poluentes Atmosféricos/farmacologia , Dióxido de Carbono/farmacologia , Carbono/análise , Florestas , Ozônio/farmacologia , Árvores/efeitos dos fármacos , Acer/efeitos dos fármacos , Acer/crescimento & desenvolvimento , Betula/efeitos dos fármacos , Betula/crescimento & desenvolvimento , Biomassa , Ecossistema , Modelos Teóricos , Solo/química , Árvores/crescimento & desenvolvimento , Estados UnidosRESUMO
Gene expression responses of paper birch (Betula papyrifera) leaves to elevated concentrations of CO(2) and O(3) were studied with microarray analyses from three time points during the summer of 2004 at Aspen FACE. Microarray data were analyzed with clustering techniques, self-organizing maps, K-means clustering and Sammon's mappings, to detect similar gene expression patterns within sampling times and treatments. Most of the alterations in gene expression were caused by O(3), alone or in combination with CO(2). O(3) induced defensive reactions to oxidative stress and earlier leaf senescence, seen as decreased expression of photosynthesis- and carbon fixation-related genes, and increased expression of senescence-associated genes. The effects of elevated CO(2) reflected surplus of carbon that was directed to synthesis of secondary compounds. The combined CO(2)+O(3) treatment resulted in differential gene expression than with individual gas treatments or in changes similar to O(3) treatment, indicating that CO(2) cannot totally alleviate the harmful effects of O(3).
Assuntos
Poluentes Atmosféricos/toxicidade , Betula/genética , Dióxido de Carbono/metabolismo , Expressão Gênica/efeitos dos fármacos , Ozônio/toxicidade , Folhas de Planta/genética , Betula/efeitos dos fármacos , Betula/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Análise de Sequência com Séries de Oligonucleotídeos , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/crescimento & desenvolvimentoRESUMO
Early spring leaf out is important to the success of deciduous trees competing for light and space in dense forest plantation canopies. In this study, we investigated spring leaf flush and how long-term growth at elevated carbon dioxide concentration ([CO(2)]) and elevated ozone concentration ([O(3)]) altered leaf area index development in a closed Populus tremuloides (aspen) canopy. This work was done at the Aspen FACE experiment where aspen clones have been grown since 1997 in conditions simulating the [CO(2)] and [O(3)] predicted for approximately 2050. The responses of two clones were compared during the first month of spring leaf out when CO(2) fumigation had begun, but O(3) fumigation had not. Trees in elevated [CO(2)] plots showed a stimulation of leaf area index (36%), while trees in elevated [O(3)] plots had lower leaf area index (-20%). While individual leaf area was not significantly affected by elevated [CO(2)], the photosynthetic operating efficiency of aspen leaves was significantly improved (51%). There were no significant differences in the way that the two aspen clones responded to elevated [CO(2)]; however, the two clones responded differently to long-term growth at elevated [O(3)]. The O(3)-sensitive clone, 42E, had reduced individual leaf area when grown at elevated [O(3)] (-32%), while the tolerant clone, 216, had larger mature leaf area at elevated [O(3)] (46%). These results indicate a clear difference between the two clones in their long-term response to elevated [O(3)], which could affect competition between the clones, and result in altered genotypic composition in future atmospheric conditions.
Assuntos
Poluentes Atmosféricos/metabolismo , Dióxido de Carbono/metabolismo , Ozônio/metabolismo , Folhas de Planta/metabolismo , Populus/metabolismo , Clorofila/metabolismo , Células Clonais , Glucose/metabolismo , Folhas de Planta/crescimento & desenvolvimento , Populus/crescimento & desenvolvimento , Estações do Ano , Estresse FisiológicoRESUMO
Betula papyrifera trees were exposed to elevated concentrations of CO(2) (1.4 x ambient), O(3) (1.2 x ambient) or CO(2) + O(3) at the Aspen Free-air CO(2) Enrichment Experiment. The treatment effects on leaf surface characteristics were studied after nine years of tree exposure. CO(2) and O(3) increased epidermal cell size and reduced epidermal cell density but leaf size was not altered. Stomatal density remained unaffected, but stomatal index increased under elevated CO(2). Cuticular ridges and epicuticular wax crystallites were less evident under CO(2) and CO(2) + O(3). The increase in amorphous deposits, particularly under CO(2) + O(3,) was associated with the appearance of elongated plate crystallites in stomatal chambers. Increased proportions of alkyl esters resulted from increased esterification of fatty acids and alcohols under elevated CO(2) + O(3). The combination of elevated CO(2) and O(3) resulted in different responses than expected under exposure to CO(2) or O(3) alone.
Assuntos
Poluentes Atmosféricos/toxicidade , Betula/efeitos dos fármacos , Dióxido de Carbono/metabolismo , Ozônio/toxicidade , Epiderme Vegetal/efeitos dos fármacos , Folhas de Planta/efeitos dos fármacos , Betula/citologia , Betula/metabolismo , Epiderme Vegetal/citologia , Epiderme Vegetal/metabolismo , Folhas de Planta/citologia , Folhas de Planta/metabolismoRESUMO
The effect of high temperature on photosynthesis of isoprene-emitting (aspen) and non-isoprene-emitting (birch) trees were measured under elevated CO(2) and ambient conditions. Aspen trees tolerated heat better than birch trees and elevated CO(2) protected photosynthesis of both species against moderate heat stress. Elevated CO(2) increased carboxylation capacity, photosynthetic electron transport capacity, and triose phosphate use in both birch and aspen trees. High temperature (36-39 degrees C) decreased all of these parameters in birch regardless of CO(2) treatment, but only photosynthetic electron transport and triose phosphate use at ambient CO(2) were reduced in aspen. Among the two aspen clones tested, 271 showed higher thermotolerance than 42E possibly because of the higher isoprene-emission, especially under elevated CO(2). Our results indicate that isoprene-emitting trees may have a competitive advantage over non-isoprene emitting ones as temperatures rise, indicating that biological diversity may be affected in some ecosystems because of heat tolerance mechanisms.
Assuntos
Betula/metabolismo , Dióxido de Carbono/metabolismo , Temperatura Alta , Populus/metabolismo , Estresse Fisiológico/efeitos dos fármacos , Betula/efeitos dos fármacos , Aquecimento Global , Fotossíntese/efeitos dos fármacos , Transpiração Vegetal/efeitos dos fármacos , Populus/efeitos dos fármacosRESUMO
Photosynthetic acclimation under elevated carbon dioxide (CO(2)) and/or ozone (O(3)) has been the topic of discussion in many papers recently. We examined whether or not aspen plants grown under elevated CO(2) and/or O(3) will acclimate after 11 years of exposure at the Aspen Face site in Rhinelander, WI, USA. We studied diurnal patterns of instantaneous photosynthetic measurements as well as A/C(i) measurements monthly during the 2004-2008 growing seasons. Our results suggest that the responses of two aspen clones differing in O(3) sensitivity showed no evidence of photosynthetic and stomatal acclimation under either elevated CO(2), O(3) or CO(2) + O(3). Both clones 42E and 271 did not show photosynthetic nor stomatal acclimation under elevated CO(2) and O(3) after a decade of exposure. We found that the degree of increase or decrease in the photosynthesis and stomatal conductance varied significantly from day to day and from one season to another.
Assuntos
Aclimatação/efeitos dos fármacos , Poluentes Atmosféricos/toxicidade , Dióxido de Carbono/metabolismo , Ozônio/toxicidade , Fotossíntese/efeitos dos fármacos , Populus/metabolismo , Estômatos de Plantas/efeitos dos fármacos , Estômatos de Plantas/metabolismo , Populus/efeitos dos fármacosRESUMO
The effects of elevated concentrations of atmospheric tropospheric ozone (O(3)) on DNA damage in five trembling aspen (Populus tremuloides Michx.) clones growing in a free-air enrichment experiment in the presence and absence of elevated concentrations of carbon dioxide (CO(2)) were examined. Growing season mean hourly O(3) concentrations were 36.3 and 47.3 ppb for ambient and elevated O(3) plots, respectively. The 4th highest daily maximum 8-h ambient and elevated O(3) concentrations were 79 and 89 ppb, respectively. Elevated CO(2) averaged 524 ppm (+150 ppm) over the growing season. Exposure to O(3) and CO(2) in combination with O(3) increased DNA damage levels above background as measured by the comet assay. Ozone-tolerant clones 271 and 8L showed the highest levels of DNA damage under elevated O(3) compared with ambient air; whereas less tolerant clone 216 and sensitive clones 42E and 259 had comparably lower levels of DNA damage with no significant differences between elevated O(3) and ambient air. Clone 8L was demonstrated to have the highest level of excision DNA repair. In addition, clone 271 had the highest level of oxidative damage as measured by lipid peroxidation. The results suggest that variation in cellular responses to DNA damage between aspen clones may contribute to O(3) tolerance or sensitivity.
Assuntos
Poluentes Atmosféricos/toxicidade , Dano ao DNA , Ozônio/toxicidade , Populus/genética , Dióxido de Carbono/metabolismo , Células Clonais , Ensaio Cometa , Reparo do DNA/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Populus/efeitos dos fármacos , Fatores de TempoRESUMO
The balance of mechanistic detail with mathematical simplicity contributes to the broad use of the Farquhar, von Caemmerer and Berry (FvCB) photosynthetic rate model. Here the FvCB model was coupled with a stomatal conductance model to form an [A,g(s)] model, and parameterized for mature Populus tremuloides leaves under varying CO(2) and temperature levels. Data were selected to be within typical forest light, CO(2) and temperature ranges, reducing artifacts associated with data collected at extreme values. The error between model-predicted photosynthetic rate (A) and A data was measured in three ways and found to be up to three times greater for each of two independent data sets than for a base-line evaluation using parameterization data. The evaluation methods used here apply to comparisons of model validation results among data sets varying in number and distribution of data, as well as to performance comparisons of [A,g(s)] models differing in internal-process components.
Assuntos
Modelos Biológicos , Fotossíntese , Folhas de Planta/metabolismo , Populus/metabolismoRESUMO
The diurnal changes in light-saturated photosynthesis (Pn) under elevated CO(2) and/or O(3) in relation to stomatal conductance (g(s)), water potential, intercellular [CO(2)], leaf temperature and vapour-pressure difference between leaf and air (VPD(L)) were studied at the Aspen FACE site. Two aspen (Populus tremuloides Michx.) clones differing in their sensitivity to ozone were measured. The depression in Pn was found after 10:00 h. The midday decline in Pn corresponded with both decreased g(s) and decreased Rubisco carboxylation efficiency, Vc(max). As a result of increasing VPD(L), g(s) decreased. Elevated [CO(2)] resulted in more pronounced midday decline in Pn compared to ambient concentrations. Moreover, this decline was more pronounced under combined treatment compared to elevated CO(2) treatment. The positive impact of CO(2) on Pn was relatively more pronounced in days with environmental stress but relatively less pronounced during midday depression. The negative impact of ozone tended to decrease in both cases.
Assuntos
Dióxido de Carbono/metabolismo , Ritmo Circadiano/efeitos dos fármacos , Ozônio/toxicidade , Fotossíntese/efeitos dos fármacos , Populus/efeitos dos fármacos , Clorofila/metabolismo , Mudança Climática , Ozônio/metabolismo , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/metabolismo , Estômatos de Plantas/metabolismo , Populus/metabolismo , Estresse Fisiológico/efeitos dos fármacos , Água/metabolismoRESUMO
Current forest Free Air CO(2) Enrichment (FACE) experiments are reaching completion. Therefore, it is time to define the scientific goals and priorities of future experimental facilities. In this opinion article, we discuss the following three overarching issues (i) What are the most urgent scientific questions and how can they be addressed? (ii) What forest ecosystems should be investigated? (iii) Which other climate change factors should be coupled with elevated CO(2) concentrations in future experiments to better predict the effects of climate change? Plantations and natural forests can have conflicting purposes for high productivity and environmental protection. However, in both cases the assessment of carbon balance and how this will be affected by elevated CO(2) concentrations and the interacting climate change factors is the most pressing priority for future experiments.
Assuntos
Dióxido de Carbono/análise , Árvores/metabolismo , Dióxido de Carbono/metabolismo , Ecossistema , Efeito EstufaRESUMO
The effect of elevated CO(2) and O(3) on apparent quantum yield (varphi), maximum photosynthesis (P(max)), carboxylation efficiency (V(cmax)) and electron transport capacity (J(max)) at different canopy locations was studied in two aspen (Populus tremuloides) clones of contrasting O(3) tolerance. Local light climate at every leaf was characterized as fraction of above-canopy photosynthetic photon flux density (%PPFD). Elevated CO(2) alone did not affect varphi or P(max), and increased J(max) in the O(3)-sensitive, but not in the O(3)-tolerant clone. Elevated O(3) decreased leaf chlorophyll content and all photosynthetic parameters, particularly in the lower canopy, and the negative impact of O(3) increased through time. Significant interaction effect, whereby the negative impact of elevated O(3) was exaggerated by elevated CO(2) was seen in Chl, N and J(max), and occurred in both O(3)-tolerant and O(3)-sensitive clones. The clonal differences in the level of CO(2)xO(3) interaction suggest a relationship between photosynthetic acclimation and background O(3) concentration.
Assuntos
Poluentes Atmosféricos/toxicidade , Dióxido de Carbono/metabolismo , Ozônio/toxicidade , Fotossíntese/efeitos dos fármacos , Populus/metabolismo , Relação Dose-Resposta a Droga , Transporte de Elétrons/efeitos dos fármacos , Ozônio/metabolismo , Folhas de Planta/metabolismo , Populus/efeitos dos fármacos , Populus/crescimento & desenvolvimentoRESUMO
This study compared the leaf transcription profiles, physiological characteristics and primary metabolites of two Populus tremuloides genotypes (clones 216 and 271) known to differ in their responses to long-term elevated [CO2] (e[CO2]) at the Aspen free-air CO2 enrichment site near Rhinelander, WI, USA. The physiological responses of these clones were similar in terms of photosynthesis, stomatal conductance and leaf area index under e[CO2], yet very different in terms of growth enhancement (0-10% in clone 216; 40-50% in clone 271). Although few genes responded to long-term exposure to e[CO2], the transcriptional activity of leaf e[CO2]-responsive genes was distinctly different between the clones, differentially impacting multiple pathways during both early and late growing seasons. An analysis of transcript abundance and carbon/nitrogen biochemistry suggested that the CO2-responsive clone (271) partitions carbon into pathways associated with active defense/response to stress, carbohydrate/starch biosynthesis and subsequent growth. The CO2-unresponsive clone (216) partitions carbon into pathways associated with passive defense (e.g. lignin, phenylpropanoid) and cell wall thickening. This study indicates that there is significant variation in expression patterns between different tree genotypes in response to long-term exposure to e[CO2]. Consequently, future efforts to improve productivity or other advantageous traits for carbon sequestration should include an examination of genetic variability in CO2 responsiveness.
Assuntos
Dióxido de Carbono/farmacologia , Carbono/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Populus/genética , Células Clonais , Regulação da Expressão Gênica de Plantas/efeitos da radiação , Genes de Plantas/genética , Variação Genética/efeitos dos fármacos , Variação Genética/efeitos da radiação , Genótipo , Luz , Nitrogênio/metabolismo , Tamanho do Órgão/efeitos dos fármacos , Tamanho do Órgão/efeitos da radiação , Fotossíntese/efeitos dos fármacos , Fotossíntese/efeitos da radiação , Folhas de Planta/efeitos da radiação , Caules de Planta/anatomia & histologia , Caules de Planta/efeitos dos fármacos , Caules de Planta/efeitos da radiação , Populus/efeitos dos fármacos , Populus/fisiologia , Populus/efeitos da radiação , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estações do Ano , Transcrição Gênica/efeitos dos fármacos , Transcrição Gênica/efeitos da radiaçãoRESUMO
Isoprene is the most important nonmethane hydrocarbon emitted by plants. The role of isoprene in the plant is not entirely understood but there is evidence that it might have a protective role against different oxidative stresses originating from heat shock and/or exposure to ozone (O(3)). Thus, plants under stress conditions might benefit by constitutively high or by higher stress-induced isoprene emission rates. In this study, measurements are presented of isoprene emission from aspen (Populus tremuloides) trees grown in the field for several years under elevated CO(2) and O(3). Two aspen clones were investigated: the O(3)-tolerant 271 and the O(3)-sensitive 42E. Isoprene emission decreased significantly both under elevated CO(2) and under elevated O(3) in the O(3)-sensitive clone, but only slightly in the O(3)-tolerant clone. This study demonstrates that long-term-adapted plants are not able to respond to O(3) stress by increasing their isoprene emission rates. However, O(3)-tolerant clones have the capacity to maintain higher amounts of isoprene emission. It is suggested that tolerance to O(3) is explained by a combination of different factors; while the reduction of O(3) uptake is likely to be the most important, the capacity to maintain higher amounts of isoprene is an important factor in strengthening this character.
Assuntos
Butadienos/metabolismo , Dióxido de Carbono/farmacologia , Hemiterpenos/metabolismo , Oxidantes Fotoquímicos/farmacologia , Ozônio/farmacologia , Pentanos/metabolismo , Populus/metabolismo , Adaptação Fisiológica , Dióxido de Carbono/metabolismo , Clonagem Molecular , Oxidantes Fotoquímicos/metabolismo , Estresse Oxidativo , Ozônio/metabolismo , Fotossíntese/efeitos dos fármacos , Populus/efeitos dos fármacos , Populus/genéticaRESUMO
A rising global population and demand for protein-rich diets are increasing pressure to maximize agricultural productivity. Rising atmospheric [CO(2)] is altering global temperature and precipitation patterns, which challenges agricultural productivity. While rising [CO(2)] provides a unique opportunity to increase the productivity of C(3) crops, average yield stimulation observed to date is well below potential gains. Thus, there is room for improving productivity. However, only a fraction of available germplasm of crops has been tested for CO(2) responsiveness. Yield is a complex phenotypic trait determined by the interactions of a genotype with the environment. Selection of promising genotypes and characterization of response mechanisms will only be effective if crop improvement and systems biology approaches are closely linked to production environments, that is, on the farm within major growing regions. Free air CO(2) enrichment (FACE) experiments can provide the platform upon which to conduct genetic screening and elucidate the inheritance and mechanisms that underlie genotypic differences in productivity under elevated [CO(2)]. We propose a new generation of large-scale, low-cost per unit area FACE experiments to identify the most CO(2)-responsive genotypes and provide starting lines for future breeding programmes. This is necessary if we are to realize the potential for yield gains in the future.
Assuntos
Dióxido de Carbono/metabolismo , Produtos Agrícolas/fisiologia , Abastecimento de Alimentos , Projetos de Pesquisa , Aclimatação , Ar , Produtos Agrícolas/genética , Genótipo , Efeito Estufa , Fenótipo , Fotossíntese/fisiologiaRESUMO
We studied the effects of long-term exposure (nine years) of birch (Betula papyrifera) trees to elevated CO(2) and/or O(3) on reproduction and seedling development at the Aspen FACE (Free-Air Carbon Dioxide Enrichment) site in Rhinelander, WI. We found that elevated CO(2) increased both the number of trees that flowered and the quantity of flowers (260% increase in male flower production), increased seed weight, germination rate, and seedling vigor. Elevated O(3) also increased flowering but decreased seed weight and germination rate. In the combination treatment (elevated CO(2)+O(3)) seed weight is decreased (20% reduction) while germination rate was unaffected. The evidence from this study indicates that elevated CO(2) may have a largely positive impact on forest tree reproduction and regeneration while elevated O(3) will likely have a negative impact.
Assuntos
Poluentes Atmosféricos/toxicidade , Betula/fisiologia , Dióxido de Carbono/toxicidade , Agricultura Florestal , Oxidantes Fotoquímicos/toxicidade , Ozônio/toxicidade , Betula/efeitos dos fármacos , Biomassa , Monitoramento Ambiental/métodos , Topos Floridos/efeitos dos fármacos , Topos Floridos/crescimento & desenvolvimento , Germinação , Efeito Estufa , Reprodução , Plântula , Sementes , Tempo , WisconsinRESUMO
We investigated the interactive effects of elevated concentrations of carbon dioxide ([CO(2)]) and ozone ([O(3)]) on radial growth, wood chemistry and structure of five 5-year-old trembling aspen (Populus tremuloides Michx.) clones and the wood chemistry of paper birch (Betula papyrifera Marsh.). Material for the study was collected from the Aspen FACE (free-air CO(2) enrichment) experiment in Rhinelander, WI, where the saplings had been exposed to four treatments: control, elevated [CO(2)] (560 ppm), elevated [O(3)] (1.5 x ambient) and their combination for five growing seasons. Wood properties of both species were altered in response to exposure to the treatments. In aspen, elevated [CO(2)] decreased uronic acids (constituents of, e.g., hemicellulose) and tended to increase stem diameter. In response to elevated [O(3)] exposure, acid-soluble lignin concentration decreased and vessel lumen diameter tended to decrease. Elevated [O(3)] increased the concentration of acetone-soluble extractives in paper birch, but tended to decrease the concentration of these compounds in aspen. In paper birch, elevated [CO(2)] decreased and elevated [O(3)] increased starch concentration. The responses of wood properties to 5 years of fumigation differed from those previously reported after 3 years of fumigation.
Assuntos
Betula/efeitos dos fármacos , Dióxido de Carbono/farmacologia , Ozônio/farmacologia , Populus/efeitos dos fármacos , Betula/crescimento & desenvolvimento , Betula/metabolismo , Parede Celular/efeitos dos fármacos , Parede Celular/metabolismo , Populus/crescimento & desenvolvimento , Populus/metabolismo , Ácidos Urônicos/metabolismoRESUMO
Paper birch (Betula papyrifera Marsh.) and three trembling aspen clones (Populus tremuloides Michx.) were studied to determine if alterations in carbon gain in response to an elevated concentration of CO(2) ([CO(2)]) or O(3) ([O(3)]) or a combination of both affected bud size and carbohydrate composition in autumn, and early leaf development in the following spring. The trees were measured for gas exchange, leaf size, date of leaf abscission, size and biochemical characteristics of the overwintering buds and early leaf development during the 8th-9th year of free-air CO(2) and O(3) exposure at the Aspen FACE site located near Rhinelander, WI. Net photosynthesis was enhanced 49-73% by elevated [CO(2)], and decreased 13-30% by elevated [O(3)]. Elevated [CO(2)] delayed, and elevated [O(3)] tended to accelerate, leaf abscission in autumn. Elevated [CO(2)] increased the ratio of monosaccharides to di- and oligosaccharides in aspen buds, which may indicate a lag in cold acclimation. The total carbon concentration in overwintering buds was unaffected by the treatments, although elevated [O(3)] decreased the amount of starch by 16% in birch buds, and reduced the size of aspen buds, which may be related to the delayed leaf development in aspen during the spring. Elevated [CO(2)] generally ameliorated the effects of elevated [O(3)]. Our results show that both elevated [CO(2)] and elevated [O(3)] have the potential to alter carbon metabolism of overwintering buds. These changes may cause carry-over effects during the next growing season.
Assuntos
Betula/crescimento & desenvolvimento , Dióxido de Carbono/farmacologia , Carbono/metabolismo , Flores/efeitos dos fármacos , Flores/fisiologia , Ozônio/farmacologia , Populus/crescimento & desenvolvimento , Betula/efeitos dos fármacos , Betula/efeitos da radiação , Células Clonais , Flores/efeitos da radiação , Luz , Tamanho do Órgão/efeitos dos fármacos , Tamanho do Órgão/efeitos da radiação , Fotossíntese/efeitos dos fármacos , Fotossíntese/efeitos da radiação , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/fisiologia , Folhas de Planta/efeitos da radiação , Estômatos de Plantas/efeitos dos fármacos , Estômatos de Plantas/efeitos da radiação , Populus/efeitos dos fármacos , Populus/efeitos da radiação , Estações do Ano , Solo , Temperatura , Fatores de Tempo , ÁguaRESUMO
Coupled surface-atmosphere models are being used with increased frequency to make predictions of tropospheric chemistry on a 'future' earth characterized by a warmer climate and elevated atmospheric CO2 concentration. One of the key inputs to these models is the emission of isoprene from forest ecosystems. Most models in current use rely on a scheme by which global change is coupled to changes in terrestrial net primary productivity (NPP) which, in turn, is coupled to changes in the magnitude of isoprene emissions. In this study, we conducted measurements of isoprene emissions at three prominent global change experiments in the United States. Our results showed that growth in an atmosphere of elevated CO2 inhibited the emission of isoprene at levels that completely compensate for possible increases in emission due to increases in aboveground NPP. Exposure to a prolonged drought caused leaves to increase their isoprene emissions despite reductions in photosynthesis, and presumably NPP. Thus, the current generation of models intended to predict the response of isoprene emission to future global change probably contain large errors. A framework is offered as a foundation for constructing new isoprene emission models based on the responses of leaf biochemistry to future climate change and elevated atmospheric CO2 concentrations.
Assuntos
Atmosfera , Butadienos , Ecossistema , Efeito Estufa , Hemiterpenos , Pentanos , Árvores , Modelos Teóricos , Estados UnidosRESUMO
Atmospheric CO2 and tropospheric O3 are rising in many regions of the world. Little is known about how these two commonly co-occurring gases will affect reproductive fitness of important forest tree species. Here, we report on the long-term effects of CO2 and O3 for paper birch seedlings exposed for nearly their entire life history at the Aspen FACE (Free Air Carbon Dioxide Enrichment) site in Rhinelander, WI. Elevated CO2 increased both male and female flower production, while elevated O3 increased female flower production compared to trees in control rings. Interestingly, very little flowering has yet occurred in combined treatment. Elevated CO2 had significant positive effect on birch catkin size, weight, and germination success rate (elevated CO2 increased germination rate of birch by 110% compared to ambient CO2 concentrations, decreased seedling mortality by 73%, increased seed weight by 17%, increased root length by 59%, and root-to-shoot ratio was significantly decreased, all at 3 weeks after germination), while the opposite was true of elevated O3 (elevated O3 decreased the germination rate of birch by 62%, decreased seed weight by 25%, and increased root length by 15%). Under elevated CO2, plant dry mass increased by 9 and 78% at the end of 3 and 14 weeks, respectively. Also, the root and shoot lengths, as well as the biomass of the seedlings, were increased for seeds produced under elevated CO2, while the reverse was true for seedlings from seeds produced under the elevated O3. Similar trends in treatment differences were observed in seed characteristics, germination, and seedling development for seeds collected in both 2004 and 2005. Our results suggest that elevated CO2 and O3 can dramatically affect flowering, seed production, and seed quality of paper birch, affecting reproductive fitness of this species.
Assuntos
Betula/crescimento & desenvolvimento , Dióxido de Carbono/farmacologia , Ozônio/farmacologia , Atmosfera , Betula/efeitos dos fármacos , Betula/embriologia , Flores/efeitos dos fármacos , Flores/crescimento & desenvolvimento , Germinação/efeitos dos fármacos , Efeito Estufa , Reprodução/efeitos dos fármacos , Sementes/efeitos dos fármacos , Sementes/crescimento & desenvolvimento , ÁrvoresRESUMO
Emission of hydrocarbons by trees has a crucial role in the oxidizing potential of the atmosphere. In particular, isoprene oxidation leads to the formation of tropospheric ozone and other secondary pollutants. It is expected that changes in the composition of the atmosphere will influence the emission rate of isoprene, which may in turn feedback on the accumulation of pollutants and greenhouse gases. We investigated the isoprene synthase (ISPS) gene expression and the ISPS protein levels in aspen trees exposed to elevated ozone (O(3)) and/or elevated carbon dioxide (CO(2)) in field-grown trees at the Aspen Free-Air Carbon Dioxide Enrichment (FACE) experimental site. Elevated O(3) reduced ISPS mRNA and the amount of ISPS protein in aspen leaves, whereas elevated CO(2) had no significant effect. Aspen clones with different O(3) sensitivity showed different levels of inhibition under elevated O(3) conditions. The drop in ISPS protein levels induced a drop in the isoprene emission rate under elevated O(3). However, the data indicated that other mechanisms also contributed to the observed strong inhibition of isoprene emission under elevated O(3).