RESUMO
Educators have become increasingly committed to social and emotional learning in schools. However, we know too little about the typical growth trajectories of the competencies that schools are striving to improve. We leverage data from the California Office to Reform Education, a consortium of districts in California serving over 1.5 million students, that administers annual surveys to students to measure social and emotional competencies (SECs). This article uses data from six cohorts of approximately 16,000 students each (51% male, 73% Latinx, 11% White, 10% Black, 24% with parents who did not complete high school) in Grades 4-12. Two questions are addressed. First, how much growth occurs in growth mindset, self-efficacy, self-management, and social awareness from Grades 4 to 12? Second, do initial status and growth look different by parental educational attainment and gender? Using accelerated longitudinal design growth models, findings show distinct growth trends among the four SECs with growth mindset increasing, self-management mostly decreasing, and self-efficacy and social awareness decreasing and then increasing. The subgroup analyses show gaps between groups but patterns of growth that are more similar than different. Further, subgroup membership accounts for very little variation in growth or declines. Instead, initial levels of competencies predict growth. Also, variation within groups is greater than variation between groups. The findings have practical implications for educators and psychologists striving to improve SECs. If schools use student-report approaches, predicting steady and consistent positive growth in SECs is unrealistic. Instead, U-shaped patterns for some SECs appear to be normative with notable declines in the sixth grade, requiring new supports. (PsycInfo Database Record (c) 2024 APA, all rights reserved).
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Given the nature of horse-human interactions in equine-assisted services (EAS), death of a horse may have significant impacts. In this study, an online survey was distributed to EAS practitioners. The goal of the study was to explore the experiences of practitioners and identify the socioemotional processes that occur upon the death of an equine within an EAS program. Open-ended responses (N = 84) were analyzed qualitatively using a grounded theory and constant-comparative approach. Responses are situated into two themes (1) Processing the death of an equine and (2) Practical implications. Experiences processing the death of an equine can be situated within Worden's Four Tasks of Mourning, extending a grief model previously only considered in the context of human death to animal death. Practical implications found within responses highlight actions practitioners can take to prepare for and process through the death of an equine in their program.
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Abstract The aim of this review is to present an update on the susceptibility of viridans groupstreptococci (VGS) to -lactam antimicrobials, with emphasis on the Argentinean scenario. VGSare a heterogeneous group including five groups of species, each one exhibiting peculiar sus-ceptibility patterns to penicillin (PEN). Species of the Streptococcus mitis group are frequentlynonsusceptible to PEN. PEN resistance is associated with changes in PEN-binding proteins. InArgentina, one to two thirds of VGS are nonsusceptible to PEN. Third generation cephalosporinsand carbapenems are currently more effective in vitro than PEN against VGS. Mortality was asso-ciated to nonsusceptibility to PEN in at least two studies involving patients with bacteremiacaused by VGS. Treatment of endocarditis due to VGS should be adjusted/to the PEN suscepti-bility of the isolates. Vancomycin may be an alternative choice for treating endocarditis causedby PEN-resistant isolates (MIC 4 g/ml).
Resumen El objetivo de esta revisión es presentar una actualización sobre la sensibilidad de los estreptococos del grupo viridans (EGV) a los antimicrobianos p-lactámicos, con énfasis en el escenario argentino. Los EGV son un grupo heterogéneo que incluye cinco grupos de especies, y cada una presenta su patrón especial de sensibilidad a la penicilina (PEN). Las especies del grupo Streptococcus mitis, con mayor frecuencia, no son sensibles a la PEN. La resistencia a la PEN se asocia con cambios de las proteínas ligadoras de PEN. En la Argentina, de uno a dos tercios de los EGV no son sensibles a la PEN. Las cefalosporinas de tercera generación y los carbapenemes son actualmente más eficaces in vitro que la PEN contra los EGV. La mortalidad se asoció con la no sensibilidad a la PEN en al menos dos estudios de pacientes con bacteriemia por EGV. El tratamiento de las endocarditis por EGV debe ajustarse según la sensibilidad a la PEN de los aislados. La vancomicina podría ser una elección alternativa para el tratamiento de las endocarditis por cepas resistentes a PEN (CIM >4 ^g/ml).
RESUMO
The aim of this review is to present an update on the susceptibility of viridans group streptococci (VGS) to ß-lactam antimicrobials, with emphasis on the Argentinean scenario. VGS are a heterogeneous group including five groups of species, each one exhibiting peculiar susceptibility patterns to penicillin (PEN). Species of the Streptococcus mitis group are frequently nonsusceptible to PEN. PEN resistance is associated with changes in PEN-binding proteins. In Argentina, one to two thirds of VGS are nonsusceptible to PEN. Third generation cephalosporins and carbapenems are currently more effective in vitro than PEN against VGS. Mortality was associated to nonsusceptibility to PEN in at least two studies involving patients with bacteremia caused by VGS. Treatment of endocarditis due to VGS should be adjusted/to the PEN susceptibility of the isolates. Vancomycin may be an alternative choice for treating endocarditis caused by PEN-resistant isolates (MIC≥4µg/ml).
Assuntos
Endocardite , Infecções Estreptocócicas , Humanos , Testes de Sensibilidade Microbiana , Infecções Estreptocócicas/tratamento farmacológico , Estreptococos Viridans , Penicilinas , Monobactamas , beta-Lactamas/farmacologia , beta-Lactamas/uso terapêutico , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Endocardite/tratamento farmacológicoRESUMO
This study provides empirical benchmarks that quantify typical changes in students' reports of social and emotional skills in a large, diverse sample. Data come from six cohorts of students (N = 361,815; 6% Asian, 8% Black, 68% White, 75% Latinx, 50% Female) who responded to the CORE survey from 2015 to 2018 and help quantify typical gains/declines in growth mindset, self-efficacy, self-management, and social awareness. Results show fluctuations in skills between 4th and 12th grade (changes ranging from -.33 to .23 standard deviations). Growth mindset increases in fourth grade, declines in fifth to seventh grade, then mostly increases. Self-efficacy, self-management, and social awareness decline in sixth to eighth grade. Self-management and social awareness, but not self-efficacy, show increases in 10th to 12th grade.
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Benchmarking , Estudantes , Emoções , Feminino , Humanos , Masculino , Autoeficácia , Habilidades Sociais , Estudantes/psicologia , Inquéritos e QuestionáriosRESUMO
Streptococcus pneumoniae is an important causal agent of pneumonia, meningitis, sepsis, bacteremia, and otitis media. Penicillin resistance rates in S. pneumoniae have remained stable in Argentina in the last years. In the late '90s more isolates with MIC of penicillin ≥2µg/ml were observed; however, their frequency has decreased in recent years. The phenotypic expression of penicillin resistance is due to a modification in penicillin-binding proteins associated with a mosaic structure in the coding genes. The expansion of successful resistant clones varies among the different regions and is influenced by the use of antibiotics, vaccines, particularly conjugated ones, as well as population density. Parenteral treatment with high doses of penicillin G continues to be effective for the treatment of pneumonia and bacteremia, oral aminopenicillins for otitis media and sinusitis and third generation cephalosporins for meningitis.
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Infecções Pneumocócicas , Streptococcus pneumoniae , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Argentina , Humanos , Testes de Sensibilidade Microbiana , Resistência às Penicilinas , Infecções Pneumocócicas/tratamento farmacológico , Streptococcus pneumoniae/genética , beta-Lactamas/farmacologiaRESUMO
The goals of the current study were twofold. The first goal was to describe levels of mathematics and science self-efficacy and achievement among a sample of students with varying levels of English language proficiency. The second goal was to examine the extent to which students' self-efficacy explains the relation between their English proficiency level and mathematics and science achievement. The sample consisted of 332 fifth graders (mean age = 10.46 years, SD = 0.38) and their 63 teachers in 20 schools. The student sample was linguistically diverse with parents reporting 22 different home languages. Based on district classification procedures, each student was coded into one of three English language proficiency level categories: English proficient-speaking students (English proficient), English Learner (EL) students who are reaching proficiency, yet are still being monitored (reaching proficiency), and EL students who are receiving English for Speakers of Other Languages services (ESOL; limited English proficient). Regression analyses indicated that students identified as limited English proficient consistently demonstrated lower achievement and self-efficacy across the content areas of mathematics and science as compared to their peers who were English proficient and reaching proficiency. In addition, students' self-efficacy partially explained the relation between limited English proficiency level and achievement for science, but not for mathematics. Results indicate that educators should consider variability in students' English proficiency levels as they select supports to promote both science achievement and self-efficacy. Findings also suggest promise for practices and programs that foster self-efficacy in addition to language and content skills.
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Sucesso Acadêmico , Idioma , Aprendizagem , Matemática/educação , Ciência/educação , Autoeficácia , Estudantes/psicologia , Criança , Feminino , Humanos , Proficiência Limitada em Inglês , MasculinoRESUMO
Enterococci are intrinsically resistant to several antimicrobial classes and show a great ability to acquire new mechanisms of resistance. Resistance to β-lactam antibiotics is a major concern because these drugs either alone or in combination are commonly used for the treatment of enterococcal infections. Ampicillin resistance, which is rare in Enterococcus faecium occurs in most of the hospital-associated Enterococcus faecium isolates. High-level resistance to ampicillin in E. faecium is mainly due to the enhanced production of PBP5 and/or by polymorphisms in the beta subunit of this protein. The dissemination of high-level ampicillin resistance can be the result of both clonal spread of strains with mutated pbp5 genes and resistance horizontal gene transfer.
Los enterococos son intrínsecamente resistentes a varias clases de antimicrobianos y presentan una gran capacidad para adquirir mecanismos de resistencia. La resistencia a los antibióticos p-lactámicos es preocupante porque estos fármacos solos o combinados se usan comúnmente para el tratamiento de las infecciones enterocócicas. La mayoría de los aislamientos hospitalarios de Enterococcus faecium presentan resistencia a la ampicilina, la cual es rara en Enterococcus faecalis. El alto nivel de resistencia a la ampicilina en E. faecium se debe principalmente a la hiperproducción de PBP5 y/o a polimorfismos en la subunidad beta de esta proteína. La propagación de esta resistencia puede deberse tanto a la diseminación clonal de cepas con genes pbp5 mutados como a la transferencia horizontal de genes.
Assuntos
Enterococcus faecium/efeitos dos fármacos , Enterococcus faecium/genética , Farmacorresistência Bacteriana/genética , Ampicilina/antagonistas & inibidores , Resistência a Ampicilina/genéticaRESUMO
Las micobacterias de crecimiento rápido son una rara causa de endocarditis bacteriana. Durante las últimas décadas han aumentado las infecciones debido a este tipo de micobacterias, en especial las postraumáticas y las posquirúrgicas. Estas infecciones pueden ser localizadas o diseminadas, y también pueden producir brotes nosocomiales debido a la contaminación del equipamiento médico. Por lo general, las tinciones para bacterias ácido-alcohol resistentes no se emplean de rutina en el procesamiento de hemocultivos positivos. Sin embargo, el microbiólogo debe estar atento al ver un bacilo gram positivo, ya que podría tratarse de una micobacteria de crecimiento rápido. Describimos un caso de endocarditis por de Mycobacterium mageritense en una paciente con parche pericárdico autógeno y un catéter para medir la presión en la aurícula izquierda. La bacteria fue identificada por espectrometría de masas (MALDI-TOF MS), score 2,3, y luego confirmada por secuenciación y análisis del gen ARNr 16s con las bases de datos del NCBI y EzTaxon, con una concordancia del 99,8 y el 100%, respectivamente.
Rapidly growing non-tuberculosis mycobacteria are a rare cause of bacterial endocarditis. During the last decades, there has been an increase in infections due to rapidly growing mycobacteria, mainly after trauma and post-surgical procedures, both localized and disseminated, as well as nosocomial outbreaks due to contamination of medical equipment. Routine acid-fast staining for blood culture bottles is not always performed; however, the microbiologist should be aware of potential RGM infections especially when gram positive bacilli are observed. We describe a case of endocarditis caused by Mycobacterium mageritense in a patient with an autologous pericardial patch and a pressure catheter in the left auricle. The bacterial species was identified as Mycobacterium mageritense by mass spectrometry (MALDI-TOF MS), score 2.3, and confirmed by 16S rRNA analysis with 99.8 and 100% agreement, respectively.
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Humanos , Feminino , Adulto , Endocardite Bacteriana/microbiologia , Infecções Relacionadas a Cateter/diagnóstico , Mycobacterium/isolamento & purificação , Espectrometria de Massas/métodos , RNA Ribossômico 16S/análise , Infecções Relacionadas a Cateter/terapia , Hemocultura/métodosRESUMO
Rapidly growing non-tuberculosis mycobacteria are a rare cause of bacterial endocarditis. During the last decades, there has been an increase in infections due to rapidly growing mycobacteria, mainly after trauma and post-surgical procedures, both localized and disseminated, as well as nosocomial outbreaks due to contamination of medical equipment. Routine acid-fast staining for blood culture bottles is not always performed; however, the microbiologist should be aware of potential RGM infections especially when gram positive bacilli are observed. We describe a case of endocarditis caused by Mycobacterium mageritense in a patient with an autologous pericardial patch and a pressure catheter in the left auricle. The bacterial species was identified as Mycobacterium mageritense by mass spectrometry (MALDI-TOF MS), score 2.3, and confirmed by 16S rRNA analysis with 99.8 and 100% agreement, respectively.
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Infecções por Actinomycetales/microbiologia , Cateterismo Cardíaco/instrumentação , Endocardite Bacteriana/microbiologia , Mycobacteriaceae , Infecções Relacionadas à Prótese/etiologia , Adulto , Feminino , HumanosRESUMO
Enterococci are intrinsically resistant to several antimicrobial classes and show a great ability to acquire new mechanisms of resistance. Resistance to ß-lactam antibiotics is a major concern because these drugs either alone or in combination are commonly used for the treatment of enterococcal infections. Ampicillin resistance, which is rare in Enterococcus faecalis, occurs in most of the hospital-associated Enterococcus faecium isolates. High-level resistance to ampicillin in E. faecium is mainly due to the enhanced production of PBP5 and/or by polymorphisms in the beta subunit of this protein. The dissemination of high-level ampicillin resistance can be the result of both clonal spread of strains with mutated pbp5 genes and horizontal gene transfer.
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Enterococcus/efeitos dos fármacos , beta-Lactamas/farmacologia , Farmacorresistência Bacteriana , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Humanos , Lactobacillales , Testes de Sensibilidade Microbiana , beta-Lactamas/uso terapêuticoRESUMO
Group A (GAS), B (GBS), c (GCS) and G (GGS) β-hemolytic streptococci are important human pathogens. They cause infections of different severity and frequency. Nowadays, after 70 years of use, penicillin is still universally active against GAS, GCS and GGS. However, therapeutic failures have been recorded in 2-28% of pharyngitis cases (median: 12%) attributable to different causes. By contrast, some GBS with reduced susceptibility to penicillin have been described, especially in Japan. In this group of bacteria, it is important to highlight that confirmation by reference methods is mandatory when decreased susceptibility to penicillin is suspected as well as checked for the detection of the mechanisms involved.
Los estreptococos β-hemolíticos de los grupos A (GAS), B (GBS), C (GCS) y G (GGS) son importantes patógenos humanos. Ellos producen infecciones de diversa gravedad y frecuencia. Aún después de más de 70 años de uso, la penicilina sigue siendo activa in vitro frente al 100% de los GAS, GCS y GGS. No obstante se han producido fallas terapéuticas entre el 2-28% de los casos de faringitis (media: 12%), atribuibles a diversas causas. En cambio se han descrito aislados de GBS con sensibilidad reducida a la penicilina, especialmente en Japón. Es importante que toda sospecha de sensibilidad disminuida a la penicilina en este grupo de bacterias sea confirmada por los métodos de referencia y comprobada mediante la detección de los mecanismos involucrados.
Assuntos
Humanos , Streptococcus/efeitos dos fármacos , beta-Lactamas/farmacologia , Testes de Sensibilidade Microbiana , Farmacorresistência BacterianaRESUMO
Group A (GAS), B (GBS), C (GCS) and G (GGS) ß-hemolytic streptococci are important human pathogens. They cause infections of different severity and frequency. Nowadays, after 70 years of use, penicillin is still universally active against GAS, GCS and GGS. However, therapeutic failures have been recorded in 2-28% of pharyngitis cases (median: 12%) attributable to different causes. By contrast, some GBS with reduced susceptibility to penicillin have been described, especially in Japan. In this group of bacteria, it is important to highlight that confirmation by reference methods is mandatory when decreased susceptibility to penicillin is suspected as well as checked for the detection of the mechanisms involved.
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Streptococcus/efeitos dos fármacos , beta-Lactamas/farmacologia , Farmacorresistência Bacteriana , Humanos , Testes de Sensibilidade MicrobianaRESUMO
Mycoplasma hominis is a fastidious bacterium, which usually colonizes the lower urogenital tract and may cause systemic infections in neonates and genital infections in adults. It can also be the cause of serious extra-genital infections, mainly in immunosuppressed or predisposed subjects. CASE PRESENTATION: We describe a case of bacteremia caused by M. hominis in a previously healthy woman after uterine curettage due to incomplete abortion. M. hominis could be an underestimated cause of bacteremia in immunocompetent patients. Mycoplasma organisms have fastidious growth requirements, are often difficult to culture on a cell-free medium and have no cell wall. The conventional method for detection may fail. This is the first report of M. hominis isolation from a positive automated blood culture (BD BACTEC, USA).
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Bacteriemia , Infecções por Mycoplasma , Mycoplasma hominis , Infecções Urinárias , Adulto , Bacteriemia/diagnóstico , Feminino , Humanos , Mycoplasma , Infecções por Mycoplasma/diagnóstico , Mycoplasma hominis/isolamento & purificação , Mycoplasma hominis/patogenicidade , Gravidez , Infecções Urinárias/diagnósticoRESUMO
BACKGROUND: Globally, Latin America ranks third among regions with most cases of AIDS related cryptococcal meningitis. In 2009, a lateral flow immunoassay (LFA) for the detection of cryptococcal antigen (CrAg) was developed as a potential point-of-care test for diagnosis of cryptococcal infection. In 2011 World Health Organizations recommended on CrAg screening for HIV positive persons with CD4 below 100 cells/µL, followed by preemptive fluconazole treatment. However, in Argentina no formal recommendations for CrAg screening have been issued. METHODS: HIV positive patients > = 18 years with advanced immunosuppression (CD4 counts ≤100 cells/µL within 3 months or WHO stage III/IV), who visited the hospital between April 1, 2014 and January 31, 2015, were included. The LFA was performed according to the manufacturer's instructions on all serum samples. When CrAg detection was positive, a lumbar puncture was performed to rule out cryptococcal meningitis. Patients without evidence of meningeal involvement were treated with preemptive oral fluconazole in ambulatory care. RESULTS: We included 123 patients. Prevalence of CrAg-positivity was 8.1%. Among the 10 CrAg-positive patients, 6 had meningeal involvement detected through the CSF analysis (CSF India-ink testing, CSF CrAg and culture). The remaining 4 patients with positive CrAg received targeted preemptive treatment with oral fluconazole and were free of cryptococcal disease during the follow-up period. None of the 113 patients with a negative CrAg test result developed cryptococcal disease. CONCLUSIONS: This is the first study in Argentina, to our knowledge, describing the prevalence of cryptococcosis and usefulness of CrAg screening. LFA provided early diagnosis to determine a high prevalence of CrAg in our hospital, and that screening for subclinical infection with preemptive antifungal treatment, prevented a substantial proportion of meningeal disease.
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Antígenos de Fungos/sangue , Fluconazol/uso terapêutico , Infecções por HIV/complicações , Meningite Criptocócica/diagnóstico , Meningite Criptocócica/epidemiologia , Adulto , Argentina/epidemiologia , Feminino , Infecções por HIV/sangue , Humanos , Imunoensaio , Masculino , Pessoa de Meia-Idade , Sistemas Automatizados de Assistência Junto ao Leito , Prevalência , Estudos Prospectivos , Punção EspinalRESUMO
School-based social and emotional learning (SEL) programs are presented to educators with little understanding of the program components that have the greatest leverage for improving targeted outcomes. Conducted in the context of a randomized controlled trial, the present study used variation in treatment teachers' (N = 143) implementation of four core components of the Responsive Classroom approach to examine relations between each component and the quality of teachers' emotional, organizational, and instructional interactions in third, fourth, and fifth grade classrooms (controlling for pre-intervention interaction quality and other covariates). We also examined the extent to which these relations varied as a function of teachers' baseline levels of interaction quality. Indices of teachers' implementation of Morning Meeting, Rule Creation, Interactive Modeling, and Academic Choice were derived from a combination of teacher-reported surveys and classroom observations. Ratings of teacher-student classroom interactions were aggregated across five observations conducted throughout the school year. Structural path models indicated that teachers' use of Morning Meeting and Academic Choice related to higher levels of emotionally supportive interactions; Academic Choice also related to higher levels of instructional interactions. In addition, teachers' baseline interaction quality moderated several associations such that the strongest relations between RC component use and interaction quality emerged for teachers with the lowest baseline interaction quality. Results highlight the value of examining individual program components toward the identification of program active ingredients that can inform intervention optimization and teacher professional development.
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Relações Interpessoais , Professores Escolares , Aprendizado Social , Estudantes/psicologia , Criança , Estudos de Coortes , Ajustamento Emocional , Feminino , Humanos , Masculino , Mid-Atlantic Region , Observação , Inquéritos e QuestionáriosRESUMO
Theory suggests that African American students benefit from warm and demanding teachers. This study examines the relation between students' perceptions of 634 teachers' warm demander characteristics and achievement growth in fourth and fifth grades (Mstudent age = 9-11.5 years). Analyses explored whether relations were moderated by the proportion of African American students in the classroom or the ethnic match or mismatch between African American students and their teachers. Results indicated that students' perceptions of teachers' demand (challenge and control) related to student achievement growth. Findings showed a stronger relation between challenge and academic growth in classrooms with more African American students, but no significant findings were identified for ethnic match or mismatch.
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Sucesso Acadêmico , Negro ou Afro-Americano/psicologia , Relações Interpessoais , Professores Escolares/psicologia , Criança , Feminino , Humanos , Masculino , Instituições Acadêmicas , Percepção Social , Estados UnidosRESUMO
La infección por VIH incrementa la posibilidad de contraer tuberculosis; el riesgo resulta mayor para las formas extrapulmonares y dentro de estas, la meníngea. La infección por VIH no modifica las manifestaciones neurológi-cas pero disminuye significativamente la supervivencia. El método molecu-lar GeneXpert MTB/RIF (CEPHEID, USA), implementado en 2010, es un mé-todo de amplificación del ácido nucleico para Mycobacterium tuberculosis, en tiempo real, con la determinación adicional de la resistencia a rifampicina.Caso clínico: paciente con VIH de diagnóstico reciente que manifes-tó enfermedad consuntiva. El cuadro inicial fue de compromiso pulmo-nar y luego meníngeo; en ambos casos el método GeneXpert MTB/RIF detectó Mycobacterium tuberculosis resistente a rifampicina. Se utili-zaron drogas de segunda línea, por la presunción de estar frente a una tuberculosis multiresistente, por definición resistente al menos a iso-niacida y rifampicina. Desarrolló hidrocefalia y profundas secuelas.Conclusiones: este método molecular permitió la detección rápida de la tuberculosis, la implementación de medidas de aislamiento así como con-trol de la infección y el inicio temprano de una terapéutica más eficaz, tan-to en la forma pulmonar como meníngea
HIV infection increases the risk of developing any form of tuberculosis, but the risk is greater for meningitis and the extrapulmonary form. HIV infection does not alter the neurological manifestations, but significantly decreases survival. The GeneXpert MTB / RIF (CEPHEID, USA), implemented in 2010, is a real time PCR for Mycobacterium tuberculosis, with rifampicin sensitivity.Case report: patient with recent diagnosed HIV, stating wasting disease. The original picture involved lung and then meningeal compromise: In both cases the Xpert MTB / RIF detected the presence of Mycobacterium tuberculosis rimfapicin resistant. Second-line drugs were used by the presumption of being faced with multidrug-resistant tuberculosis that, by definition, is resistant to at least isoniazid and rifampin. Patient developed hydrocephalus and was severe aftermath.Conclusions: This molecular method allowed the rapid detection of tuberculosis, implementation of isolation measures as infection control and the early onset of a more effective therapy, both in the pulmonary form and the meningeal
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Humanos , Masculino , Adulto , Tuberculose Meníngea/terapia , Infecções por HIV/terapia , Controle de Infecções/métodos , Técnicas de Amplificação de Ácido NucleicoRESUMO
Rapid identification of microorganisms is critical in hospitalized infected patients. Blood culture is currently the gold standard for detecting and identifying microorganisms causing bacteremia or sepsis. The introduction of mass spectrometry by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF MS) in microbiology laboratories, especially in microorganisms growing in blood culture bottles, provides rapid identification. This study evaluates the performance of the Maldi Sepsityper Biotyper procedure (hereinafter, MS) compared to that of an in-home method (hereinafter, HF). Eight hundred and forty (840) positive blood culture bottles were processed using the HF procedure, 542 of which were also processed using MS. The organisms were identified in 670 (79.76%) and 391 (72.14%) bottles respectively (p = 0,0013). This study demonstrates the effectiveness of both procedures for identifying microorganisms directly from positive blood culture bottles. However, the HF procedure proved to be more effective than MS, especially in the presence of Gram positive organisms.