Integrated Structural Modulation Inducing Fast Charge Transfer in Aqueous Zinc-Ion Batteries.

Aqueous Zn-ion batteries (AZIBs) are promising energy-storage devices owing to their exceptional safety, long cycle life, simple production, and high storage capacity. Manganese oxides are considered potential cathode materials for AZIBs, primarily because of their safety, low cost, simple synthesis, and high storage capacity. However, MnO2-based cathodes tend to deteriorate structurally during long-term cycling, which reduces their reversible capacity. In this study, an advanced α-MnO2@SnO2 nanocomposite via facile hydrothermal synthesis is developed. The synergistic effects of lattice disorder and increased electron conductivity in the α-MnO2@SnO2 nanocomposite mitigate structural degradation and enhance the overall electrochemical performance. The nanocomposite exhibits a high reversible capacity of 347 mAh g-1 at a current density of 100 mA g-1 after 50 cycles. Furthermore, it exhibits excellent rate performance and stable capacity even after 1000 cycles, maintaining a capacity of 78 mAh g-1 at a high current density of 5 A g-1. This excellent electrochemical performance is attributed to the reversible Zn intercalation in α-MnO2@SnO2 nanocomposites due to the increased structural stability and fast ion/electron exchange caused by the distortion of the tunnel structure, on the basis of various ex situ experiments, density functional theory calculations, and electrochemical characterizations.

Looking inside the black box of gender differences in creativity: A dual-process model and meta-analysis.

Although prior work has characterized creativity as a primarily agentic endeavor, we diverge from this perspective and argue for agentic and communal pathways to creativity that offer unique advantages to each gender. We draw from social role theory to predict that risk-taking and empathic tendencies-as agentic and communal mechanisms, respectively-help explain how gender influences creativity. We also identify contextual moderators that can strengthen the communal pathway-predicting a more positive relationship between empathic tendency and creativity as well as a stronger indirect effect via empathic tendency when the tasks demand perspective-taking and when usefulness is explicitly incorporated in creativity assessment. With a meta-analysis of 753 independent samples (265,762 individuals), we find support for a communal pathway (i.e., women are creative via empathic tendency) and for an agentic pathway (i.e., men are creative via risk-taking tendency). We also find that the communal pathway is stronger when usefulness is explicitly incorporated in creativity assessment. Task demands for perspective-taking did not show a moderating effect. Taken together, our findings provide a more balanced account of the gender-creativity relationship, demonstrate why men and women differ in creativity and when women can leverage the communal mechanism to enhance creativity, and inform theory and practice towards a more gender-equitable workplace. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Presynaptic structural and functional plasticity are coupled by convergent Rap1 signaling.

Dynamic presynaptic actin remodeling drives structural and functional plasticity at synapses, but the underlying mechanisms remain largely unknown. Previous work has shown that actin regulation via Rac1 guanine exchange factor (GEF) Vav signaling restrains synaptic growth via bone morphogenetic protein (BMP)-induced receptor macropinocytosis and mediates synaptic potentiation via mobilization of reserve pool vesicles in presynaptic boutons. Here, we find that Gef26/PDZ-GEF and small GTPase Rap1 signaling couples the BMP-induced activation of Abelson kinase to this Vav-mediated macropinocytosis. Moreover, we find that adenylate cyclase Rutabaga (Rut) signaling via exchange protein activated by cAMP (Epac) drives the mobilization of reserve pool vesicles during post-tetanic potentiation (PTP). We discover that Rap1 couples activation of Rut-cAMP-Epac signaling to Vav-mediated synaptic potentiation. These findings indicate that Rap1 acts as an essential, convergent node for Abelson kinase and cAMP signaling to mediate BMP-induced structural plasticity and activity-induced functional plasticity via Vav-dependent regulation of the presynaptic actin cytoskeleton.

Spherical Sulfur-Infiltrated Carbon Cathode with a Tunable Poly(3,4-ethylenedioxythiophene) Layer for Lithium-Sulfur Batteries.

Li-S batteries have received significant attention owing to their high energy density, nontoxicity, low cost, and eco-friendliness. However, the dissolution of lithium polysulfide during the charge/discharge process and its extremely low electron conductivity hinder practical applications of Li-S batteries. Herein, we report a sulfur-infiltrated carbon cathode material with a spherical morphology and conductive polymer coating. The material was produced via a facile polymerization process that forms a robust nanostructured layer and physically prevents the dissolution of lithium polysulfide. The thin double layer composed of carbon and poly(3,4-ethylenedioxythiophene) provides sufficient space for sulfur storage and effectively prevents the elution of polysulfide during continuous cycling, thereby playing an essential role in increasing the sulfur utilization rate and significantly improving the electrochemical performance of the battery. Sulfur-infiltrated hollow carbon spheres with a conductive polymer layer demonstrate a stable cycle life and reduced internal resistance. The as-fabricated battery demonstrated an excellent capacity of 970 mA h g-1 at 0.5 C and a stable cycle performance, exhibiting ∼78% of the initial discharge capacity after 50 cycles. This study provides a promising approach to significantly improve the electrochemical performance of Li-S batteries and render them as valuable and safe energy devices for large-scale energy storage systems.

Disagreement Between the Accepted Best-Fit Circle Method to Calculate Bone Loss Between Injured and Uninjured Shoulders.

BACKGROUND: No study has evaluated whether best-fit circles based on glenoids with defects accurately represent normal inferior glenoids before injury. PURPOSE: To investigate whether the best-fit circles on the affected side with a glenoid defect can accurately represent native glenoids before injury. STUDY DESIGN: Cohort study (diagnosis); Level of evidence, 3. METHODS: This retrospective study included 58 patients with unilateral recurrent anterior shoulder instability. First, we compared the diameter of best-fit circles based on affected and unaffected glenoids. Glenoid defect sizes based on each best-fit circle were then calculated and compared. Second, we created serial virtual glenoid defects (10%, 15%, 20%, 25%) on unaffected glenoids and compared diameters of best-fit circles on the glenoids before and after virtual defects. We also analyzed and compared the size of virtual and calculated glenoid defects. Bland-Altman plots and intraclass coefficients (ICCs) were used to compare and analyze agreement of measurements. After categorization of glenoid defects based on clinical cutoff values, Cohen κ and percentage agreement were calculated. RESULTS: The diameter of 55.2% (32/58) of best-fit circles from affected glenoids over- or underestimated the diameter on the unaffected side by >5%. In 28 of the 32 patients, the diameter of the affected side circle was overestimated. Consequently, 41.4% (24/58) of glenoid defects were over- or underestimated by >5%. In 19 of the 24 patients, the glenoid defect from the affected side was >5% larger. ICCs between sides for best-fit circle diameters and defect sizes were 0.632 and 0.800, respectively. Agreement of glenoid defect size between sides was 58.6% (34/58) overall, but when the defect was ≥10%, agreement decreased to 32.3% (10/31). Among 232 glenoids with virtual defects created from 58 normal glenoids, the diameter of 31.0% (72/232) of best-fit circles and the size of 11.6% (27/232) of defects were over- or underestimated by >5%. CONCLUSION: When assessing glenoid defects in anterior shoulder instability, best-fit circles based on affected glenoids do not always represent the native glenoid and may thus lead to inaccurate circle sizes and defect estimates.

Subscapularis tendon tears hidden by the medial biceps sling can be missed on arthroscopic examination.

INTRODUCTION: To investigate (1) the prevalence of "hidden lesions" and "non-hidden lesions" of subscapularis tendon tears requiring repair during arthroscopic examination that would be missed by a 30° arthroscope, but could be identified by a 70° arthroscope, from the standard posterior portal and (2) the correlation of preoperative internal rotation weakness and findings of magnetic resonance imaging (MRI) indicating hidden lesions. MATERIALS AND METHODS: We retrospectively examined 430 patients who underwent arthroscopic subscapularis repair between was initially nonvisible with a 30° arthroscope but became visible only with a 70° arthroscope from the standard posterior portal. The preoperative and intraoperative findings of the hidden lesion group (n = 82) were compared with those of the non-hidden lesion group (n = 348). 2016 and 2020. A hidden lesion was defined as a subscapularis tendon tear requiring repair that preoperative internal rotation weakness was assessed using the modified belly-press test. Preoperative MR images were reviewed using a systemic approach. RESULTS: The prevalence of hidden lesions was 19.1% (82/430). No significant difference was found in preoperative internal rotation weakness between the groups. Preoperative MRI showed a significantly lower detection rate in the hidden lesion group than in the non-hidden group (69.5% vs. 84.8%; P = 0.001). The hidden lesions were at a significantly earlier stage of subscapularis tendon tears than the non-hidden lesions, as revealed by the arthroscopic findings (Lafosse classification, degree of retraction; P = 0.003 for both) and MR findings (muscle atrophy, fatty infiltration; P = 0.001, P = 0.005, respectively). CONCLUSIONS: Among the subscapularis tears requiring repair, 19.1% could be identified by a 70° arthroscope, but not by a 30° arthroscope, through the posterior portal. The hidden lesions showed a significantly lower detection rate on preoperative MRI than the non-hidden lesions. Thus, for subscapularis tears suspected on preoperative physical examination, the 70° arthroscope would be helpful to avoid a misdiagnosis.

High failure rate after conservative treatment for recurrent shoulder dislocation without subjective apprehension on physical examination.

PURPOSE: The purpose of this study was to investigate the outcomes of conservative treatment for recurrent shoulder dislocation without subjective apprehension, despite the presence of a Bankart lesion or glenoid defect. METHODS: A retrospective analysis was performed for 92 patients with recurrent shoulder dislocation treated with conservative treatment due to negative apprehension between 2009 and 2018. The failure of the conservative treatment was defined as a dislocation or subluxation episode or subjective feeling of instability based on a positive apprehension. The Kaplan-Meier method was used to estimate failure rates over time, and a receiver operating characteristic (ROC) curve was constructed to determine a cut-off value for a glenoid defect. The clinical outcomes were compared between patients who completed conservative treatment without recurrence of instability (Group A) and those who failed and subsequently underwent surgical treatment (Group B) using shoulder functional scores and sports/recreation activity level. RESULTS: This retrospective study included 61 of 92 eligible patients with recurrent shoulder dislocation. Among the 61 patients, conservative treatment failed in 46 (75.4%) over the 2-year study period. The cut-off value for a glenoid defect was 14.4%. The association between glenoid defect size (≥ 14.4% or as a continuous variable) and survival was statistically significant (p = 0.039 and p < 0.001, respectively). The mean glenoid defect size in Group B increased from 14.6 ± 3.0% to 17.3 ± 3.1% (p < 0.001), and clinical outcomes for Group A were inferior to those for Group B at the 24-month follow-up. CONCLUSIONS: Conservative treatment for recurrent shoulder dislocation in patients without subjective apprehension showed a high failure rate during the study period, especially if the glenoid defect was ≥ 14.4% in size. Despite clinical improvement in patients who completed conservative treatment without recurrence, functional outcome scores and sport/recreation activity levels were better in the patients who underwent arthroscopic Bankart repair. Therefore, for recurrent anterior shoulder instability, even without subjective apprehension, surgical treatment is warranted over conservative treatment. LEVEL OF EVIDENCE: Level IV.

Synthesis and Characterization of MnO2@Cellulose and Polypyrrole-Decorated MnO2@Cellulose for the Detection of Chemical Warfare Agent Simulant.

Chemical warfare agents (CWAs) have been threatening human civilization and its existence because of their rapid response, toxic, and irreversible nature. The hybrid nanostructured composites were synthesized by the hydrothermal process to detect the dimethyl methyl phosphonate (DMMP), a simulant of G-series nerve agents, especially sarin. Cellulose (CE), manganese oxide cellulose (MnO2@CE), and MnO2@CE/polypyrrole (PPy) exhibited a frequency shift of 0.4, 4.8, and 8.9 Hz, respectively, for a DMMP concentration of 25 ppm in the quartz crystal microbalance (QCM). In surface acoustic wave (SAW) sensor, they exhibited 187 Hz, 276 Hz, and 78 Hz, respectively. A comparison between CE, MnO2@CE, and MnO2@CE/PPy demonstrated that MnO2@CE/PPy possesses excellent linearity with a coefficient of determination (COD or R2) of 0.992 and 0.9547 in the QCM and SAW sensor. The hybrid composite materials showed a reversible adsorption and desorption phenomenon in the reproducibility test. The response and recovery times indicated that MnO2@CE/PPy showed the shortest response (~23 s) and recovery times (~42 s) in the case of the QCM sensor. Hence, the pristine CE and its nanostructured composites were compared to analyze the sensing performance based on sensitivity, selectivity, linearity, reproducibility, and response and recovery times to detect the simulant of nerve agents.

Nano-Sheet-like Morphology of Nitrogen-Doped Graphene-Oxide-Grafted Manganese Oxide and Polypyrrole Composite for Chemical Warfare Agent Simulant Detection.

Chemical warfare agents (CWAs) have inflicted monumental damage to human lives from World War I to modern warfare in the form of armed conflict, terrorist attacks, and civil wars. Is it possible to detect the CWAs early and prevent the loss of human lives? To answer this research question, we synthesized hybrid composite materials to sense CWAs using hydrothermal and thermal reduction processes. The synthesized hybrid composite materials were evaluated with quartz crystal microbalance (QCM) and surface acoustic wave (SAW) sensors as detectors. The main findings from this study are: (1) For a low dimethyl methyl phosphonate (DMMP) concentration of 25 ppm, manganese dioxide nitrogen-doped graphene oxide (NGO@MnO2) and NGO@MnO2/Polypyrrole (PPy) showed the sensitivities of 7 and 51 Hz for the QCM sensor and 146 and 98 Hz for the SAW sensor. (2) NGO@MnO2 and NGO@MnO2/PPy showed sensitivities of more than 50-fold in the QCM sensor and 100-fold in the SAW sensor between DMMP and potential interferences. (3) NGO@MnO2 and NGO@MnO2/PPy showed coefficients of determination (R2) of 0.992 and 0.975 for the QCM sensor and 0.979 and 0.989 for the SAW sensor. (4) NGO@MnO2 and NGO@MnO2/PPy showed repeatability of 7.00 ± 0.55 and 47.29 ± 2.69 Hz in the QCM sensor and 656.37 ± 73.96 and 665.83 ± 77.50 Hz in the SAW sensor. Based on these unique findings, we propose NGO@MnO2 and NGO@MnO2/PPy as potential candidate materials that could be used to detect CWAs.

Pull-out Strength of Suture Anchor and Torque of Buddy Anchor for an Osteoporotic Humeral Head in Rotator Cuff Repair: Parallel Versus Divergent Insertion.

BACKGROUND: The buddy anchor technique is useful to reinforce loose anchors in the osteoporotic humeral head during arthroscopic rotator cuff repair. However, theoretical parallel insertion of the buddy anchor to index a loose anchor is challenging in arthroscopy and can widen the entry site and decrease structural integrity. PURPOSE: To investigate and compare the biomechanical stability between 2 buddy anchor insertion techniques (parallel insertion vs divergent insertion) in the osteoporotic humeral head. STUDY DESIGN: Controlled laboratory study. METHODS: A total of 24 paired fresh-frozen cadaveric shoulders were used, and each pair was randomly assigned to either the parallel insertion group or the divergent insertion group. In the parallel insertion group, the buddy anchor was inserted parallel to the index loose anchor. In the divergent insertion group, the buddy anchor was inserted at a 20° angle in the medial direction to the index loose anchor. The insertion torque of the buddy anchor and ultimate pull-out strength of the index anchor were measured and compared between the 2 groups. RESULTS: The mean maximum insertion torque was significantly higher in the parallel insertion group (16.1 ± 1.8 cN·m) compared with the divergent insertion group (12.0 ± 1.5 cN·m) (P < .001). The mean ultimate pull-out strength was significantly higher with divergent insertion (192.2 ± 28.6 N) than with parallel insertion (147.7 ± 23.6 N) (P < .001). CONCLUSION: For application of the buddy anchor system in the cadaveric osteoporotic humeral bone model, divergent insertion showed better ultimate pull-out strength than conventional parallel insertion, despite inferior maximum insertion torque. CLINICAL RELEVANCE: The results of this study widen the applicability and accessibility for the buddy anchor system.

Nanorod-like Structure of ZnO Nanoparticles and Zn8O8 Clusters Using 4-Dimethylamino Benzaldehyde Liquid to Study the Physicochemical and Antimicrobial Properties of Pathogenic Bacteria.

To study their physicochemical and antimicrobial properties, zinc oxide nanoparticles were synthesized using a simple chemical route and 4-dimethylaminobenzaldehyde (4DB) as an organic additive. ZnO nanoparticles were characterized with XRD analysis, which confirmed the presence of a hexagonal wurtzite structure with different crystalline sizes. The SEM morphology of the synthesized nanoparticles confirmed the presence of nanorods in both modifications of ZnO nanoparticles. EDS analysis proved the chemical composition of the synthesized samples via different chemical approaches. In addition, the optical absorption results indicated that the use of 4DB increased the band gap energy of the synthesized nanoparticles. The synthesized Zn8O8 and Zn8O8:4DB clusters were subjected to HOMO-LUMO analysis, and their ionization energy (I), electron affinity (A), global hardness (η), chemical potential (σ), global electrophilicity index (ω), dipole moment (µ), polarizability (αtot), first-order hyperpolarizability (ßtot), and other thermodynamic properties were determined. Furthermore, the antimicrobial properties of the ZnO nanoparticles were studied against G+ (S. aureus and B. subtilis) and G- (K. pneumoniae and E. coli) bacteria in a nutrient agar according to guidelines of the Clinical and Laboratory Standards Institute (CLSI).

Eliciting Specific Electrochemical Reaction Behavior by Rational Design of a Red Phosphorus Electrode for Sodium-Ion Batteries.

Due to the demand to upgrade from lithium-ion batteries (LIB), sodium-ion batteries (SIB) have been paid considerable attention for their high-energy, cost-effective, and sustainable battery system. Red phosphorus is one of the most promising anode candidates for SIBs, with a high theoretical specific capacity of 2596 mAh g-1 and in the discharge potential range of 0.01-0.8 V; however, it suffers from a low electrical conductivity, a substantial expansion of volume (~300%), and sluggish electron/ion kinetics. Herein, we have designed a well-defined electrode, which consists of red phosphorus, nanowire arrays encapsulated in the vertically aligned carbon nanotubes (P@C NWs), which were fabricated via a two-step, anodized-aluminum oxide template. The designed anode achieved a high specific capacity of 2250 mAh g-1 (87% of the theoretical capacity), and a stepwise analysis of the reaction behavior between sodium and red phosphorus was demonstrated, both of which have not been navigated in previous studies. We believe that our rational design of the red phosphorus electrode elicited the specific reaction mechanism revealed by the charge-discharge profiles, rendered excellent electrical conductivity, and accommodated volume expansion through the effective nano-architecture, thereby suggesting an efficient structure for the phosphorus anode to advance in the future.

Improving Room Temperature Ionic Conductivity of Na3-xKxZr2Si2PO12 Solid-Electrolytes: Effects of Potassium Substitution.

The battery safety and cost remain major challenges for developing next-generation rechargeable batteries. All-solid-state sodium (Na)-ion batteries are a promising option for low-cost as well as safe rechargeable batteries by using abundant resources and solid electrolytes. However, the operation of solid-state batteries is limited due to the low ionic conductivity of solid electrolytes. Therefore, it is essential to develop new compounds that feature a high ionic conductivity and chemical stability at room temperature. Herein, we report a potassium-substituted sodium superionic conductor solid electrolyte, Na3-xKxZr2Si2PO12 (0 ≤ x ≤ 0.2), that exhibits an ionic conductivity of 7.734 × 10-4 S/cm-1 at room temperature, which is more than 2 times higher than that of the undoped sample. The synchrotron powder diffraction patterns with Rietveld refinements revealed that the substitution of large K-ions resulted in an increased unit cell volume, widened the Na diffusion channel, and shortened the Na-Na distance. Our work demonstrates that substituting a larger cation on the Na site effectively widens the ion diffusion channel and consequently increases the bulk ionic conductivity. Our findings will contribute to improving the ionic conductivity of the solid electrolytes and further developing safe next-generation rechargeable batteries.

Clinical factors affecting the longevity of fixed retainers and the influence of fixed retainers on periodontal health in periodontitis patients: a retrospective study.

PURPOSE: The aim of this study was to evaluate clinical factors affecting the longevity of fixed retainers and the influence of fixed retainers on periodontal health in periodontitis patients. METHODS: In total, 52 patients with at least 2 years of follow-up after periodontal and orthodontic treatment were included in this study. After scaling and root planing, orthodontic treatment with fixed appliances or clear aligners was performed. Fixed retainers with twist-flex stainless steel wires were bonded to the palatal or lingual sides of anterior teeth. Changes in clinical parameters, including the plaque index, gingival index, calculus index (CI), probing pocket depth, and radiographic bone levels, were evaluated before bonding of fixed retainers and at a 12-month follow-up. Cumulative survival rates (CSRs) for retainer failure were evaluated according to sex, site, CI, stage of periodontitis, and the severity of the irregularity with the log-rank test and hazard ratios (HRs). RESULTS: Twelve months after bonding of fixed retainers, improvements were observed in all clinical parameters except CI and radiographic bone gain. The overall CSR of the retainers with a CI <1 at the 12-month follow-up after bonding of fixed retainers was significantly higher than that of the retainers with a CI ≥1 at the 12-month follow-up (log-rank test; P<0.001). Patients with stage III (grade B or C) periodontitis had a higher multivariate HR for retainer failure (5.4; 95% confidence interval, 1.22-23.91; P=0.026) than patients with stage I (grade A or B) periodontitis. CONCLUSIONS: Although fixed retainers were bonded in periodontitis patients, periodontal health was well maintained if supportive periodontal treatment with repeated oral hygiene education was provided. Nonetheless, fixed retainer failure occurred more frequently in patients who had stage III (grade B or C) periodontitis or a CI ≥1 at 12-month follow-up after bonding of fixed retainers.

Highly Sensitive Hybrid Nanostructures for Dimethyl Methyl Phosphonate Detection.

Nanostructured materials synthesized by the hydrothermal and thermal reduction process were tested to detect the dimethyl methylphosphonate (DMMP) as a simulant for chemical warfare agents. Manganese oxide nitrogen-doped graphene oxide with polypyrrole (MnO2@NGO/PPy) exhibited the sensitivity of 51 Hz for 25 ppm of DMMP and showed the selectivity of 1.26 Hz/ppm. Nitrogen-doped multi-walled carbon nanotube (N-MWCNT) demonstrated good linearity with a correlation coefficient of 0.997. A comparison between a surface acoustic wave and quartz crystal microbalance sensor exhibited more than 100-times higher sensitivity of SAW sensor than QCM sensor.

First Clinical Cases of Spirometrosis in Two Cats in Korea.

This study reports the first two clinical cases of spirometrosis caused by Spirometra sp. in cats in Korea. In these two cases, the cats vomited, and long proglottids of tapeworm were recovered. The sick cats presented with anorexia and lethargy. However, they unexpectedly showed no diarrhea, which is the main symptom of spirometrosis. Based on a fecal floatation test as well as morphological and molecular analyses, the parasite was diagnosed as Spirometra sp. The 2 cases were treated with praziquantel. This study suggests regular monitoring of health and deworming in companion animals, even when animals are well cared for, with regular preventive medication. Additionally, spirometrosis should be considered in the differential diagnosis in cases of gastrointestinal symptoms in Spirometra endemic areas.

Drosophila Graf regulates mushroom body ß-axon extension and olfactory long-term memory.

Loss-of-function mutations in the human oligophrenin-1 (OPHN1) gene cause intellectual disability, a prevailing neurodevelopmental condition. However, the role OPHN1 plays during neuronal development is not well understood. We investigated the role of the Drosophila OPHN1 ortholog Graf in the development of the mushroom body (MB), a key brain structure for learning and memory in insects. We show that loss of Graf causes abnormal crossing of the MB ß lobe over the brain midline during metamorphosis. This defect in Graf mutants is rescued by MB-specific expression of Graf and OPHN1. Furthermore, MB α/ß neuron-specific RNA interference experiments and mosaic analyses indicate that Graf acts via a cell-autonomous mechanism. Consistent with the negative regulation of epidermal growth factor receptor (EGFR)-mitogen-activated protein kinase (MAPK) signaling by Graf, activation of this pathway is required for the ß-lobe midline-crossing phenotype of Graf mutants. Finally, Graf mutants have impaired olfactory long-term memory. Our findings reveal a role for Graf in MB axon development and suggest potential neurodevelopmental functions of human OPHN1.

Resistance Temperature Detectors Fabricated via Dual Fused Deposition Modeling of Polylactic Acid and Polylactic Acid/Carbon Black Composites.

Planar-type resistance temperature detectors (P-RTDs) were fabricated via fused deposition modeling by dual nozzle extrusion. The temperature-sensing element of the fabricated sensor was printed with electrically conductive polylactic acid/carbon black (PLA/CB) composite, while the structural support was printed with a PLA insulator. The temperature-dependent resistivity change of PLA/CB was evaluated for different stacking sequences of PLA/CB layers printed with [0°/0°], [-45°/45°], and [0°/90°] plies. Compared to a PLA/CB filament used as 3D printing source material, the laminated structures exhibited a response over 3 times higher, showing a resistivity change from -10 to 40 Ω∙cm between -15 and 50 °C. Then, using the [0°/90°] plies stacking sequence, a P-RTD thermometer was fabricated in conjunction with a Wheatstone bridge circuit for temperature readouts. The P-RTD yielded a temperature coefficient of resistance of 6.62 %/°C with high stability over repeated cycles. Fabrication scalability was demonstrated by realizing a 3 × 3 array of P-RTDs, allowing the temperature profile detection of the surface in contact with heat sources.

ALS2 regulates endosomal trafficking, postsynaptic development, and neuronal survival.

Mutations in the human ALS2 gene cause recessive juvenile-onset amyotrophic lateral sclerosis and related motor neuron diseases. Although the ALS2 protein has been identified as a guanine-nucleotide exchange factor for the small GTPase Rab5, its physiological roles remain largely unknown. Here, we demonstrate that the Drosophila homologue of ALS2 (dALS2) promotes postsynaptic development by activating the Frizzled nuclear import (FNI) pathway. dALS2 loss causes structural defects in the postsynaptic subsynaptic reticulum (SSR), recapitulating the phenotypes observed in FNI pathway mutants. Consistently, these developmental phenotypes are rescued by postsynaptic expression of the signaling-competent C-terminal fragment of Drosophila Frizzled-2 (dFz2). We further demonstrate that dALS2 directs early to late endosome trafficking and that the dFz2 C terminus is cleaved in late endosomes. Finally, dALS2 loss causes age-dependent progressive defects resembling ALS, including locomotor impairment and brain neurodegeneration, independently of the FNI pathway. These findings establish novel regulatory roles for dALS2 in endosomal trafficking, synaptic development, and neuronal survival.