RESUMO
A fundamental question in developmental biology is how different cell lineages acquire different cell cycle durations. With its highly stereotypical asymmetric and asynchronous cell divisions, the early Caenorhabditis elegans embryo provides an ideal system to study lineage-specific cell cycle timing regulation during development, with high spatio-temporal resolution. The first embryonic division is asymmetric and generates two blastomeres of different sizes (AB>P1) and developmental potentials that divide asynchronously, with the anterior somatic blastomere AB dividing reproducibly two minutes before the posterior germline blastomere P1. The evolutionarily conserved PAR proteins (abnormal embryonic PARtitioning of cytoplasm) regulate all of the asymmetries in the early embryo including cell cycle asynchrony between AB and P1 blastomeres. Here we discuss our current understanding and open questions on the mechanism by which the PAR proteins regulate asynchronous cell divisions in the early C. elegans embryo.
Assuntos
Proteínas de Caenorhabditis elegans/metabolismo , Caenorhabditis elegans/embriologia , Pontos de Checagem do Ciclo Celular/fisiologia , Ciclo Celular/fisiologia , Embrião não Mamífero/citologia , Animais , Caenorhabditis elegans/fisiologia , Divisão Celular , Embrião não Mamífero/metabolismoRESUMO
The spindle assembly checkpoint (SAC) prevents anaphase onset until all the chromosomes have successfully attached to the spindle microtubules. The MAP kinase (MAPK) is an important player in this pathway, however its exact role is not fully understood. One major target of MAPK is the p90 ribosomal protein S6 kinase (RSKs) family. In this study, we analyse whether Rsk2 could participate in the activation of the SAC. Our data indicate that this protein is localized at the kinetochores under checkpoint conditions. Moreover, it is essential for the SAC activity in Xenopus egg extracts as its depletion prevents metaphase arrest as well as the kinetochore localization of the other SAC components. We also show that this kinase might also participate in the maintenance of the SAC in mammalian cells as Rsk2 knockdown in these cells prevents the kinetochore localization of Mad1, Mad2 and CENP-E under checkpoint conditions.
Assuntos
Ciclo Celular , Cinetocoros/metabolismo , Proteínas Quinases S6 Ribossômicas 90-kDa/metabolismo , Fuso Acromático/metabolismo , Animais , Proteínas de Ligação ao Cálcio/metabolismo , Proteínas de Ciclo Celular/metabolismo , Proteínas Cromossômicas não Histona/metabolismo , Técnicas de Silenciamento de Genes , Células HeLa , Humanos , Proteínas Mad2 , Metáfase , Proteínas Nucleares/metabolismo , Plasmídeos/genética , Transporte Proteico , RNA Interferente Pequeno/genética , Proteínas Repressoras/metabolismo , Proteínas Quinases S6 Ribossômicas 90-kDa/deficiência , Proteínas Quinases S6 Ribossômicas 90-kDa/genética , Extratos de Tecidos/metabolismo , XenopusRESUMO
Chfr is a checkpoint protein that plays an important function in cell cycle progression and tumor suppression, although its exact role and regulation are unclear. Previous studies have utilized overexpression of Chfr to determine the signaling pathway of this protein in vivo. In this study, we demonstrate, by using three different antibodies against Chfr, that the endogenous and highly overexpressed ectopic Chfr protein is localized and regulated differently in cells. Endogenous and lowly expressed ectopic Chfr are cytoplasmic and localize to the spindle during mitosis. Higher expression of ectopic Chfr correlates with a shift in the localization of this protein to the nucleus/PML bodies, and with a block of cell proliferation. In addition, endogenous and lowly expressed ectopic Chfr is stable throughout the cell cycle, whereas when highly expressed, ectopic Chfr is actively degraded during S-G2/M phases in an autoubiquitination and proteasome-dependent manner. A two-hybrid screen identified TCTP as a possible Chfr-interacting partner. Biochemical analysis with the endogenous proteins confirmed this interaction and identified beta-tubulin as an additional partner for Chfr, supporting the mitotic spindle localization of Chfr. The Chfr-TCTP interaction was stable throughout the cell cycle, but it could be diminished by the complete depolymerization of the microtubules, providing a possible mechanism where Chfr could be the sensor that detects microtubule disruption and then activates the prophase checkpoint.
Assuntos
Biomarcadores Tumorais/fisiologia , Proteínas de Ciclo Celular/fisiologia , Proteínas de Neoplasias/fisiologia , Fuso Acromático/química , Animais , Biomarcadores Tumorais/análise , Ciclo Celular , Proteínas de Ciclo Celular/análise , Células HeLa , Humanos , Microtúbulos/fisiologia , Proteínas de Neoplasias/análise , Fosforilação , Proteínas de Ligação a Poli-ADP-Ribose , Tubulina (Proteína)/metabolismo , Proteína Tumoral 1 Controlada por Tradução , Ubiquitina-Proteína Ligases , Ubiquitinação , XenopusRESUMO
The use of Caenorhabditis elegans as a model system for understanding animal development and human disease has long been recognized as an efficient tool of discovery. Recent developments, particularly in our understanding of RNA-mediated interference and its ability to modify gene activity, have facilitated the use of C. elegans in determining gene function via high-throughput analysis. These new strategies have provided a framework that allows investigators to analyse gene function globally at the genomic level and will likely become a prototypic model for biological analysis in the post-genome era.
Assuntos
Caenorhabditis elegans/embriologia , Caenorhabditis elegans/genética , Interferência de RNA , Animais , Caenorhabditis elegans/metabolismo , Biblioteca Gênica , Genes Letais , Técnicas Genéticas , Genômica/métodos , Humanos , Neoplasias/genética , Neoplasias/metabolismoRESUMO
In most tumor cells a chromosomal instability leads to an abnormal chromosome number (aneuploidy). The mitotic checkpoint is essential for ensuring accurate chromosome segregation by allowing mitotic delay in response to a spindle defect. This checkpoint delays the onset of anaphase until all the chromosomes are correctly aligned on the mitotic spindle. When unattached kinetochores are present, the metaphase/anaphase transition is not allowed and the time available for chromosome-microtubule capture increases. Genes required for this delay were first identified in Saccharomyces cerevisiae (the MAD, BUB and MPS1 genes) and subsequently, homologs have been identified in higher eucaryotes showing that the spindle checkpoint pathway is highly conserved. The checkpoint functions by preventing an ubiquitin ligase called the anaphase-promoting complex/cyclosome (APC) from ubiquitinylating proteins whose destruction is required for anaphase onset.
Assuntos
Divisão Celular , Cromossomos/fisiologia , Aneuploidia , Animais , Segregação de Cromossomos , Humanos , Cinetocoros , Mitose , Neoplasias/genética , Saccharomyces cerevisiae/genética , Fuso AcromáticoRESUMO
CDK9 paired with cyclin T1 forms the human P-TEFb complex and stimulates productive transcription through phosphorylation of the RNA polymerase II C-terminal domain. Here we report that CDK9 is ubiquitinated and degraded by the proteasome whereas cyclin T1 is stable. SCF(SKP2) was recruited to CDK9/cyclin T1 via cyclin T1 in an interaction requiring its PEST domain. CDK9 ubiquitination was modulated by cyclin T1 and p45(SKP2). CDK9 accumulated in p45(SKP2-/-) cells, and its expression during the cell cycle was periodic. The transcriptional activity of CDK9/cyclin T1 on the class II major histocompatibility complex promoter could be regulated by CDK9 degradation in vivo. We propose a novel mechanism whereby recruitment of SCF(SKP2) is mediated by cyclin T1 while ubiquitination occurs exclusively on CDK9.
Assuntos
Quinases Ciclina-Dependentes/metabolismo , Ciclinas/metabolismo , Cisteína Endopeptidases/metabolismo , Ligases/metabolismo , Complexos Multienzimáticos/metabolismo , Complexos Ubiquitina-Proteína Ligase , Ubiquitinas/metabolismo , Ciclossomo-Complexo Promotor de Anáfase , Animais , Ciclo Celular/fisiologia , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Células Cultivadas , Ciclina T , Quinase 9 Dependente de Ciclina , Fibroblastos/metabolismo , Humanos , Camundongos , Periodicidade , Complexo de Endopeptidases do Proteassoma , Ligação Proteica/fisiologia , Estrutura Terciária de Proteína/fisiologia , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Proteínas Quinases Associadas a Fase S , Transcrição Gênica/fisiologia , Transfecção , Ubiquitina-Proteína LigasesRESUMO
Protein kinase A regulatory subunit RIIalpha is tightly bound to centrosomal structures during interphase through interaction with the A-kinase anchoring protein AKAP450, but dissociates and redistributes from centrosomes at mitosis. The cyclin B-p34(cdc2) kinase (CDK1) has been shown to phosphorylate RIIalpha on T54 and this has been proposed to alter the subcellular localization of RIIalpha. We have made stable transfectants from an RIIalpha-deficient leukemia cell line (Reh) that expresses either wild-type or mutant RIIalpha (RIIalpha(T54E)). When expressed, RIIalpha detaches from centrosomes at mitosis and dissociates from its centrosomal location in purified nucleus-centrosome complexes by incubation with CDK1 in vitro. By contrast, centrosomal RIIalpha(T54E) is not redistributed at mitosis, remains mostly associated with centrosomes during all phases of the cell cycle and cannot be solubilized by CDK1 in vitro. Furthermore, RIIalpha is solubilized from particular cell fractions and changes affinity for AKAP450 in the presence of CDK1. D and V mutations of T54 also reduce affinity for the N-terminal RII-binding domain of AKAP450, whereas small neutral residues do not change affinity detected by surface plasmon resonance. In addition, only RIIalpha(T54E) interacts with AKAP450 in a RIPA-soluble extract from mitotic cells. Finally, microtubule repolymerization from mitotic centrosomes of the RIIalpha(T54E) transfectant is poorer and occurs at a lower frequency than that of RIIalpha transfectants. Our results suggest that T54 phosphorylation of RIIalpha by CDK1 might serve to regulate the centrosomal association of PKA during the cell cycle.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteína Quinase CDC2/metabolismo , Proteínas de Transporte , Centrossomo/metabolismo , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Proteínas do Citoesqueleto , Proteínas Associadas aos Microtúbulos/metabolismo , Mitose/fisiologia , Proteínas de Ancoragem à Quinase A , Animais , Sítios de Ligação/fisiologia , Linhagem Celular/metabolismo , Centrossomo/química , Subunidade RIIalfa da Proteína Quinase Dependente de AMP Cíclico , Proteínas Quinases Dependentes de AMP Cíclico/química , Proteínas de Fluorescência Verde , Humanos , Proteínas Luminescentes/genética , Camundongos , Proteínas Associadas aos Microtúbulos/química , Microtúbulos/química , Microtúbulos/metabolismo , Fosforilação , Mutação Puntual/genética , Testes de Precipitina/métodos , Leucemia-Linfoma Linfoblástico de Células Precursoras B/metabolismo , Estrutura Terciária de Proteína/fisiologia , Ratos , Solubilidade , Frações Subcelulares/química , TransfecçãoRESUMO
The c-Mos proto-oncogene product plays an essential role during meiotic divisions in vertebrate eggs. In Xenopus, it is required for progression of oocyte maturation and meiotic arrest of unfertilized eggs. Its degradation after fertilization is essential to early embryogenesis. In this study we investigated the mechanisms involved in c-Mos degradation. We present in vivo evidence for ubiquitin-dependent degradation of c-Mos in activated eggs. We found that c-Mos degradation is not directly dependent on the anaphase-promoting factor activator Fizzy/cdc20 but requires cyclin degradation. We demonstrate that cyclin B/cdc2 controls in vivo c-Mos phosphorylation and stabilization. Moreover, we show that cyclin B/cdc2 is capable of directly phosphorylating c-Mos in vitro, inducing a similar mobility shift to the one observed in vivo. Tryptic phosphopeptide analysis revealed a practically identical in vivo and in vitro phosphopeptide map and allowed identification of serine-3 as the largely preferential phosphorylation site as previously described (Freeman et al., 1992). Altogether, these results demonstrate that, in vivo, stability of c-Mos is directly regulated by cyclin B/cdc2 kinase activity.
Assuntos
Proteína Quinase CDC2/metabolismo , Ciclina B/metabolismo , Oócitos/metabolismo , Proteínas Proto-Oncogênicas c-mos/metabolismo , Xenopus/metabolismo , Animais , Western Blotting , Estabilidade Enzimática , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Modelos Biológicos , Mapeamento de Peptídeos , Fosforilação , Testes de Precipitina , Proteínas Recombinantes/metabolismo , Ubiquitina/metabolismo , Proteínas de Xenopus/metabolismoRESUMO
The mitotic checkpoint acts to inhibit entry into anaphase until all chromosomes have successfully attached to spindle microtubules. Unattached kinetochores are believed to release an activated form of Mad2 that inhibits APC/C-dependent ubiquitination and subsequent proteolysis of components needed for anaphase onset. Using Xenopus egg extracts, a vertebrate homolog of yeast Mps1p is shown here to be a kinetochore-associated kinase, whose activity is necessary to establish and maintain the checkpoint. Since high levels of Mad2 overcome checkpoint loss in Mps1-depleted extracts, Mps1 acts upstream of Mad2-mediated inhibition of APC/C. Mps1 is essential for the checkpoint because it is required for recruitment and retention of active CENP-E at kinetochores, which in turn is necessary for kinetochore association of Mad1 and Mad2.
Assuntos
Proteínas de Transporte , Proteínas de Ciclo Celular , Ciclo Celular/fisiologia , Cinetocoros/metabolismo , Mitose/fisiologia , Oócitos/fisiologia , Proteínas Serina-Treonina Quinases/metabolismo , Proteínas Tirosina Quinases/metabolismo , Proteínas de Saccharomyces cerevisiae , Proteínas de Xenopus/metabolismo , Sequência de Aminoácidos , Animais , Proteínas de Ligação ao Cálcio/metabolismo , Núcleo Celular/fisiologia , Proteínas Cromossômicas não Histona/metabolismo , Ciclina B/genética , Ciclina B/metabolismo , Ciclina B1 , Feminino , Proteínas Fúngicas/metabolismo , Humanos , Proteínas Mad2 , Masculino , Meiose , Metáfase , Mitose/efeitos dos fármacos , Modelos Biológicos , Dados de Sequência Molecular , Nocodazol/farmacologia , Proteínas Nucleares , Oócitos/citologia , Oócitos/efeitos dos fármacos , Proteínas Quinases/metabolismo , Proteínas Serina-Treonina Quinases/genética , Proteínas Tirosina Quinases/genética , Proteínas Recombinantes/metabolismo , Reticulócitos/metabolismo , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/enzimologia , Espermatozoides/fisiologia , Ubiquitinas/metabolismo , Vertebrados , Proteínas de Xenopus/genética , Xenopus laevisRESUMO
Here we show that segregation of homologous chromosomes and that of sister chromatids are differentially regulated in Xenopus and possibly in other higher eukaryotes. Upon hormonal stimulation, Xenopus oocytes microinjected with antibodies against the anaphase-promoting complex (APC) activator Fizzy or the APC core subunit Cdc27, or with the checkpoint protein Mad2, a destruction-box peptide or methylated ubiquitin, readily progress through the first meiotic cell cycle and arrest at second meiotic metaphase. However, they fail to segregate sister chromatids and remain arrested at second meiotic metaphase when electrically stimulated or when treated with ionophore A34187, two treatments that mimic fertilization and readily induce chromatid segregation in control oocytes. Thus, APC is required for second meiotic anaphase but not for first meiotic anaphase.
Assuntos
Anáfase/fisiologia , Proteínas de Transporte , Ligases/fisiologia , Meiose/fisiologia , Oócitos/crescimento & desenvolvimento , Complexos Ubiquitina-Proteína Ligase , Proteínas de Xenopus , Xenopus/embriologia , Ciclossomo-Complexo Promotor de Anáfase , Animais , Anticorpos/farmacologia , Calcimicina/farmacologia , Proteínas de Ligação ao Cálcio/metabolismo , Proteínas de Ligação ao Cálcio/farmacologia , Proteínas Cdc20 , Proteínas de Ciclo Celular/imunologia , Proteínas de Ciclo Celular/metabolismo , Proteínas de Ciclo Celular/farmacologia , Feminino , Proteínas Fúngicas/metabolismo , Proteínas Fúngicas/farmacologia , Ionóforos/farmacologia , Microinjeções , Proteínas Nucleares , Oócitos/citologia , Oócitos/metabolismo , Progesterona/farmacologia , Ubiquitina-Proteína Ligases , Xenopus/genética , Xenopus/metabolismoRESUMO
Caenorhabditis elegans dauer formation is an alternative larval developmental pathway that the worm can take when environmental conditions become detrimental. Animals can survive several months in this stress-resistant stage and can resume normal development when growth conditions improve. Although the worms integrate a variety of sensory information to commit to dauer formation, it is currently unknown whether they also monitor internal cellular damage. The Ro ribonucleoprotein complex, which was initially described as a human autoantigen, is composed of one major 60-kDa protein, Ro60, that binds to one of four small RNA molecules, designated Y RNAs. Ro60 has been shown to bind mutant 5S rRNA molecules in Xenopus oocytes, suggesting a role for Ro60 in 5S rRNA biogenesis. Analysis of ribosomes from a C. elegans rop-1(-) strain, which is null for the expression of Ro60, demonstrated that they contain a high percentage of mutant 5S rRNA molecules, thereby strengthening the notion of a link between the rop-1 gene product and 5S rRNA quality control. The Ro particle was recently shown to be involved in the resistance of Deinococcus radiodurans to UV irradiation, suggesting a role for the Ro complex in stress resistance. We have studied the role of rop-1 in dauer formation. We present genetic and biochemical evidence that rop-1 interacts with dauer-formation genes and is involved in the regulation of the worms' entry into the dauer stage. Furthermore, we find that the rop-1 gene product undergoes a proteolytic processing step that is regulated by the dauer formation pathway via an aspartic proteinase. These results suggest that the Ro particle may function in an RNA quality-control checkpoint for dauer formation.
Assuntos
Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/crescimento & desenvolvimento , Caenorhabditis elegans/genética , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Helminto/genética , Proteínas de Helminto/metabolismo , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismo , RNA de Helmintos/genética , Animais , Caenorhabditis elegans/embriologia , Embrião não Mamífero/fisiologia , Genótipo , Larva , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/metabolismo , Receptor de Insulina/metabolismo , Fator de Crescimento Transformador beta/metabolismoRESUMO
During oogenesis, maternal mRNAs are synthesised and stored in a translationally dormant form due to the presence of regulatory elements at the 3' untranslated regions (3'UTR). In Xenopus oocytes, several studies have described the presence of RNA-binding proteins capable to repress maternal-mRNA translation. The testis-brain RNA-binding protein (TB-RBP/Translin) is a single-stranded DNA- and RNA-binding protein which can bind the 3' UTR regions (Y and H elements) of stored mRNAs and can suppress in vitro translation of the mRNAs that contain these sequences. Here we report the cloning of the Xenopus homologue of the TB-RBP/Translin protein (X-translin) as well as its expression, its localisation, and its biochemical association with the protein named Translin associated factor X (Trax) in Xenopus oocytes. The fact that this protein is highly present in the cytoplasm from stage VI oocytes until 48 h embryos and that it has been described as capable to inhibit paternal mRNA translation, indicates that it could play an important role in maternal mRNA translation control during Xenopus oogenesis and embryogenesis. Moreover, we investigated X-translin localisation during cell cycle in XTC cells. In interphase, although a weak and diffuse nuclear staining was observed, X-translin was mostly present in the cytoplasm where it exhibited a prominent granular staining. Interestingly, part of X-translin underwent a remarkable redistribution throughout mitosis and associated with centrosomes, which may suggest a new unknown role for this protein in cell cycle.
Assuntos
Centrossomo/metabolismo , Proteínas de Ligação a DNA/genética , Mitose/genética , Sequência de Aminoácidos , Animais , Proteínas de Transporte/metabolismo , Sequência Consenso , DNA Complementar/análise , DNA de Cadeia Simples/metabolismo , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/metabolismo , Humanos , Dados de Sequência Molecular , Proteínas Nucleares/metabolismo , Oócitos/metabolismo , Homologia de Sequência de Aminoácidos , Xenopus laevis/embriologiaRESUMO
Members of the polo-like family of protein kinases have been involved in the control of APC (anaphase-promoting complex) during the cell cycle, yet how they activate APC is not understood in any detail. In Xenopus oocytes, Ca2+-dependent degradation of cyclin B associated with release from arrest at second meiotic metaphase was demonstrated to require the polo-like kinase Plx1. The aim of the present study was to examine, beyond Ca2+-dependent resumption of meiosis, the possible role of Plx1 in the control of cyclin degradation during the early mitotic cell cycle. Plx1 was found to be dispensable for MPF to turn on the cyclin degradation machinery. However, it is required to prevent premature inactivation of the APC-dependent proteolytic pathway. Microcystin suppresses the requirement for Plx1 in both Ca2+-dependent exit from meiosis, associated with degradation of both cyclin B and A downstream of CaMK2 activation, and prevention of premature APC(Fizzy) inactivation in the early mitotic cell cycle. These results are consistent with the view that Plx1 antagonizes an unidentified microcystin-sensitive phosphatase that inactivates APC(Fizzy).
Assuntos
Proteínas de Ciclo Celular/metabolismo , Ligases/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Transdução de Sinais , Complexos Ubiquitina-Proteína Ligase , Proteínas de Xenopus , Ciclossomo-Complexo Promotor de Anáfase , Animais , Proteína Quinase CDC2/metabolismo , Proteínas Cdc20 , Ciclo Celular , Ciclina B/metabolismo , Cisteína Endopeptidases/metabolismo , Ativação Enzimática , Inibidores Enzimáticos/farmacologia , Humanos , Microcistinas , Complexos Multienzimáticos/metabolismo , Peptídeos Cíclicos/farmacologia , Fosfoproteínas Fosfatases/antagonistas & inibidores , Fosfoproteínas Fosfatases/metabolismo , Complexo de Endopeptidases do Proteassoma , Proteínas Serina-Treonina Quinases/genética , Estrelas-do-Mar , Fatores de Tempo , Ubiquitina-Proteína Ligases , XenopusRESUMO
Signal transduction cascades involved in regulation of the cell cycle machinery are poorly understood. In the Xenopus oocyte model, meiotic maturation is triggered by MPF, a complex of p34(cdc2)-cyclin B, which is activated in response to a progesterone signal by largely unknown mechanisms. We have previously shown that the p21-activated kinase (PAK) family negatively regulates the MPF amplification loop. In this study, we identify the endogenous PAK2 as a key enzyme in this regulation and describe the pathways by which PAK2 is regulated. We show that the small GTPase Cdc42 is required for maintenance of active endogenous X-PAK2 in resting stage VI oocytes, whereas Rac1 is not involved in this regulation. During the process of maturation, X-PAK2 phosphorylation results in its inactivation and allows maturation to proceed to completion. Activation of mitogen-activated protein kinase and cyclin B-p34(cdc2) is coincident with X-PAK2 inactivation, and purified active MPF inhibits X-PAK2, demonstrating the existence of a new positive feedback loop. Our results confirm and extend the importance of p21-activated kinases in the control of the G(2)/M transition. We hypothesize that the X-PAK2/Cdc42 pathway could link p34(cdc2) activity to the major cytoskeleton rearrangements leading to spindle migration and anchorage to the animal pole cortex.
Assuntos
Proteínas de Ciclo Celular/metabolismo , Regulação Enzimológica da Expressão Gênica , Oócitos/crescimento & desenvolvimento , Proteínas Serina-Treonina Quinases/metabolismo , Sequência de Aminoácidos , Animais , Clonagem Molecular , DNA Complementar , Feminino , Fertilização/fisiologia , Dados de Sequência Molecular , Oócitos/enzimologia , Progesterona/fisiologia , Proteínas Serina-Treonina Quinases/genética , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos , Xenopus , Quinases Ativadas por p21RESUMO
The Ro ribonucleoprotein complex (Ro RNP) was initially described as an autoimmune target in human diseases such as systemic lupus erythematosus and Sjögren's syndrome. In Xenopus and human cells, its general structure is composed of one major protein of 60 kDa, Ro60, that binds to one of four small RNA molecules, designated Y RNAs. Although no function has been assigned to the Ro RNP, Ro60 has been shown to bind mutant 5S ribosomal RNA (rRNA) molecules in Xenopus oocytes, suggesting a role for Ro60 in 5S rRNA biogenesis. Ro60 has also been shown to participate in the regulation of the translational fate of the L4 ribosomal protein mRNA by interacting with the 5' untranslated region, again suggesting its possible implication in ribosome biogenesis. To identify the function of Ro RNP, we have taken a genetic approach in the nematode Caenorhabditis elegans. As such, we characterized the gene encoding the protein ROP-1, the homologue of the human Ro60 protein. Here, we review the phenotypic analysis of C. elegans rop-l(-) mutants and integrate these results into a model for the function of the Ro RNP particle.
Assuntos
Autoantígenos/fisiologia , Caenorhabditis elegans , RNA Citoplasmático Pequeno , Ribonucleoproteínas/fisiologia , Animais , Humanos , Modelos BiológicosRESUMO
Mutations in the clk-1 gene of the nematode Caenorhabditis elegans result in an average slowing of a variety of developmental and physiological processes, including the cell cycle, embryogenesis, post-embryonic growth, rhythmic behaviors and aging. In yeast, a CLK-1 homologue is absolutely required for ubiquinone biosynthesis and thus respiration. Here we show that CLK-1 is fully active when fused to green fluorescent protein and is found in the mitochondria of all somatic cells. The activity of mutant mitochondria, however, is only very slightly impaired, as measured in vivo by a dye-uptake assay, and in vitro by the activity of succinate cytochrome c reductase. Overexpression of CLK-1 activity in wild-type worms can increase mitochondrial activity, accelerate behavioral rates during aging and shorten life span, indicating that clk-1 regulates and controls these processes. These observations also provide strong genetic evidence that mitochondria are causally involved in aging. Furthermore, the reduced respiration of the long-lived clk-1 mutants suggests that longevity is promoted by the age-dependent decrease in mitochondrial function that is observed in most species.
Assuntos
Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/genética , Caenorhabditis elegans/fisiologia , Proteínas de Helminto/genética , Proteínas de Helminto/fisiologia , Envelhecimento/genética , Envelhecimento/fisiologia , Animais , Animais Geneticamente Modificados , Comportamento Animal/fisiologia , Expressão Gênica , Genes de Helmintos , Genes Reporter , Proteínas de Fluorescência Verde , Proteínas Luminescentes/genética , Proteínas Luminescentes/metabolismo , Mitocôndrias/metabolismo , Mutação , Consumo de Oxigênio/genética , Consumo de Oxigênio/fisiologia , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismoRESUMO
We have examined the role of protein phosphorylation in the modulation of the key muscle-specific transcription factor MyoD. We show that MyoD is highly phosphorylated in growing myoblasts and undergoes substantial dephosphorylation during differentiation. MyoD can be efficiently phosphorylated in vitro by either purified cdk1-cyclin B or cdk1 and cdk2 immunoprecipitated from proliferative myoblasts. Comparative two-dimensional tryptic phosphopeptide mapping combined with site-directed mutagenesis revealed that cdk1 and cdk2 phosphorylate MyoD on serine 200 in proliferative myoblasts. In addition, when the seven proline-directed sites in MyoD were individually mutated, only substitution of serine 200 to a nonphosphorylatable alanine (MyoD-Ala200) abolished the slower-migrating hyperphosphorylated form of MyoD, seen either in vitro after phosphorylation by cdk1-cyclin B or in vivo following overexpression in 10T1/2 cells. The MyoD-Ala200 mutant displayed activity threefold higher than that of wild-type MyoD in transactivation of an E-box-dependent reporter gene and promoted markedly enhanced myogenic conversion and fusion of 10T1/2 fibroblasts into muscle cells. In addition, the half-life of MyoD-Ala200 protein was longer than that of wild-type MyoD, substantiating a role of Ser200 phosphorylation in regulating MyoD turnover in proliferative myoblasts. Taken together, our data show that direct phosphorylation of MyoD Ser200 by cdk1 and cdk2 plays an integral role in compromising MyoD activity during myoblast proliferation.
Assuntos
Proteína Quinase CDC2/metabolismo , Quinases relacionadas a CDC2 e CDC28 , Quinases Ciclina-Dependentes/metabolismo , Músculo Esquelético/citologia , Proteína MyoD/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Células-Tronco/citologia , Alanina/genética , Alanina/metabolismo , Diferenciação Celular , Divisão Celular , Quinase 2 Dependente de Ciclina , Meia-Vida , Músculo Esquelético/metabolismo , Fosforilação , Serina/metabolismo , Células-Tronco/metabolismo , Ativação TranscricionalRESUMO
The Ro ribonucleoproteins (RoRNP) consist of at least one major protein of 60 kD, Ro60, and one small associated RNA, designated Y RNA. Although RoRNP have been found in all vertebrate species examined so far, their function remains unknown. The Caenorhabditis elegans rop-1 gene previously has been identified as encoding a Ro60 homologue. We report here the phenotypic characterization of a C. elegans strain in which rop-1 has been disrupted. This is the first report regarding the inactivation of a major RoRNP constituent in any organism. The rop-1 mutant worms display no visible defects. However, at the molecular level, the disruption of rop-1 results in a dramatic decrease in the levels of the ROP-1-associated RNA (CeY RNA). Moreover, transgenic expression of wild-type rop-1 partially rescues the levels of CeY RNA. Considering that transgenes are poorly expressed in the germline, the fact that the rescue is only partial is most likely related to the high abundance of the CeY RNA in the adult germline and in embryos. The developmental expression pattern and localization of CeY RNA suggest a role for this molecule during embryogenesis. We conclude that, under laboratory culture conditions, ROP-1 does not play a crucial role in C. elegans.
Assuntos
Autoantígenos/fisiologia , Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/fisiologia , Proteínas de Helminto/fisiologia , RNA de Helmintos/metabolismo , RNA Citoplasmático Pequeno , Ribonucleoproteínas/fisiologia , Alelos , Animais , Animais Geneticamente Modificados , Autoantígenos/genética , Caenorhabditis elegans/genética , Expressão Gênica , Células Germinativas , Proteínas de Helminto/genética , Mutagênese , Fenótipo , Regiões Promotoras Genéticas , Processamento Pós-Transcricional do RNA , RNA Ribossômico 5S , Ribonucleoproteínas/genética , TransgenesRESUMO
Phosphorylation of the RII regulatory subunits of cyclic AMP-dependent protein kinases (PKAs) was examined during the HeLa cell cycle. Three RIIalpha isoforms of 51, 54, and 57 kDa were identified by RIIalpha immunodetection and labeling with 8-azido[32P]cAMP in different cell cycle phases. These isoforms were characterized as different phosphorylation states by the use of selective PKA and cyclin-directed kinase inhibitors. Whereas RIIalpha autophosphorylation by PKA caused RIIalpha to shift from 51 to 54 kDa, phosphorylation of RIIalpha by one other or a combination of several kinases activated during mitosis caused RIIalpha to shift from 51 to 57 kDa. In vivo incorporation of [32P]orthophosphate into mitotic cells and RIIalpha immunoprecipitation demonstrated that RIIalpha was hyperphosphorylated on a different site than the one phosphorylated by PKA. Deletion and mutation analysis demonstrated that the cyclin B-p34(cdc2) kinase (CDK1) phosphorylated human recombinant RIIalpha in vitro on Thr54. Whereas RIIalpha was associated with the Golgi-centrosomal region during interphase, it was dissociated from its centrosomal localization at metaphase-anaphase transition. Furthermore, particulate RIIalpha from HeLa cell extracts was solubilized following incubation with CDK1 in vitro. Our results suggest that at the onset of mitosis, CDK1 phosphorylates RIIalpha, and this may alter its subcellular localization.
Assuntos
Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Ciclo Celular/fisiologia , Marcadores de Afinidade/farmacocinética , Substituição de Aminoácidos , Anticorpos Monoclonais , Azidas/farmacocinética , Proteínas Quinases Dependentes de Cálcio-Calmodulina/química , AMP Cíclico/análogos & derivados , AMP Cíclico/farmacocinética , Proteínas Quinases Dependentes de AMP Cíclico/antagonistas & inibidores , Quinases Ciclina-Dependentes/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Células HeLa , Humanos , Substâncias Macromoleculares , Mitose , Mutagênese Sítio-Dirigida , Radioisótopos de Fósforo , Fosforilação , Reação em Cadeia da Polimerase , Proteínas Recombinantes/análise , Proteínas Recombinantes/metabolismo , Frações Subcelulares/enzimologia , TransfecçãoRESUMO
The Xenopus homologue of Drosophila Fizzy and budding yeast CDC20 has been characterized. The encoded protein (X-FZY) is a component of a high molecular weight complex distinct from the APC/cyclosome. Antibodies directed against FZY were produced and shown to prevent calmodulin-dependent protein kinase II (CaMKII) from inducing the metaphase to anaphase transition of spindles assembled in vitro in Xenopus egg extracts, and this was associated with suppression of the degradation of mitotic cyclins. The same antibodies suppressed M phase-promoting factor (MPF)-dependent activation of the APC/cyclosome in interphase egg extracts, although they did not appear to alter the pattern or extent of MPF-dependent phosphorylation of APC/cyclosome subunits. As these phosphorylations are thought to be essential for APC/cyclosome activation in eggs and early embryos, we conclude that at least two events are required for MPF to activate the APC/cyclosome, allowing both chromatid segregation and full degradation of mitotic cyclins. The first one, which does not require FZY function, is the phosphorylation of APC/cyclosome subunits. The second one, that requires FZY function (even in the absence of MAD2 protein and when the spindle assembly checkpoint is not activated) is not yet understood at its molecular level.