RESUMO
A new rapid assay for the okadaic acid group of toxins, based on lateral flow immunochromatographic (LFIC) test strips developed by Jellett Rapid Testing Ltd., was assessed on naturally contaminated bivalves from the Portuguese coast. One prototype was evaluated using samples harvested during 2005, extracted with 80% methanol, followed by dilution with the running buffer of a methanolic extract after alkaline hydrolysis for esters. The second prototype was assessed using samples harvested during 2006, extracted with 100% methanol and, after alkaline hydrolysis, the methanol was evaporated by a nitrogen stream followed by re-suspension with the running buffer. The first prototype failed to detect 20% of samples that were positive by LC-MS in the range 160-480 microg kg(-1), and were classified as negative or trace level by LFIC. The presence of methanol in the extracts made correct detection of toxins more difficult. The second prototype classified as positive all samples above 160 microg kg(-1), as confirmed by LC-MS. However, in the second prototype, matrix effects were a major drawback and led to 45% false positives, particularly for mussels, due to compounds in shellfish extracts interfering with the antibodies and reducing the test line intensity. Extraction with a higher percentage of methanol was thought responsible for these matrix effects. Regarding sample migration, both prototypes needed one hour before reading. In an attempt to speed-up sample preparation, a direct digestion of bivalve tissues with sodium hydroxide was evaluated. Low recoveries for esters were found by LC-MS with this hydrolysis technique compared to conventional hydrolysis of methanolic extracts. While prototype A was not sensitive enough, prototype B had too many false positives to be of use to the shellfish industry or in a monitoring program.
Assuntos
Bivalves/química , Carcinógenos/análise , Ésteres/análise , Ácido Okadáico/análise , Frutos do Mar/análise , Animais , Carcinógenos/química , Cromatografia/métodos , Ésteres/química , Hidrólise , Imunoensaio/métodos , Ácido Okadáico/química , PortugalRESUMO
A method is described to sulfate PSP toxins at various positions in the molecule and to prepare 35S labelled compounds using H2(35)SO4 in the presence of dicyclohexylcarbodiimide (DCC). The 11-sulfates of saxitoxin and neosaxitoxin, known as gonyautoxins, are often the most abundant of the PSP toxins in algae and contaminated shellfish. Receptor site binding and antibody assays based on these analogues should, therefore, better reflect toxicity than those in which saxitoxin is used. Although the specific activity of 35S-gonyautoxins is lower than that of commercially available 3H-saxitoxin, the label is strongly bound and is not lost through proton exchange with water as occurs with tritiated saxitoxin. The labelling procedure is rapid, inexpensive and can be done on a small scale. Sulfate can be removed from the 11-position of GTX's in methanolic-HCl and from the 21-position by mild acid hydrolysis and H2(35)SO4 added in 5-10-fold excess. Addition or exchange occurs rapidly on mixing DCC in dimethylformamide with dry toxin and sulfate. Reaction conditions were optimized and reaction products identified by capillary electrophoresis, autoradiography and ionspray mass spectrometry. Together with methods for selective removal of sulfate, the sulfation reaction provides an additional way to prepare some of the naturally occurring derivatives of saxitoxin, many of which are sulfates.
Assuntos
Marcação por Isótopo/métodos , Toxinas Marinhas/química , Saxitoxina/análogos & derivados , Sulfatos/química , Radioisótopos de Enxofre/química , Animais , Dinoflagellida/química , Eletroforese Capilar , Toxinas Marinhas/isolamento & purificação , Saxitoxina/química , Saxitoxina/isolamento & purificação , Frutos do Mar/microbiologia , Intoxicação por Frutos do MarRESUMO
A novel enzyme-linked immunosorbent assay (ELISA) technology was developed for detecting saxitoxin or evaluation of anti-saxitoxin antibodies, which is based on non-covalent immobilization "free' saxitoxin to Maxisorp microtitre plates. The effect of pH on immobilization was studied in media with wide-range buffering capacities (piperazine-glycylglycine and barbiturate buffers). Increasing pH resulted in better responses, although this was mainly due to non-specific interactions. At pH 10.0, however, saxitoxin immobilization was quite effective and specific. The same pattern was found under four different conditions; absence vs presence of bovine serum albumin precoating and absence vs presence of 150 mM NaCl. The best results (high specific response) were achieved with bovine serum albumin precoating in the presence of 150 mM NaCl. The method of choice involved precoating Maxisorp with 5 micrograms/ml albumin followed by addition of 5 microM saxitoxin in 0.01 M piperazine-glycylglycine buffer, pH 10.0. The efficacy of this technology was demonstrated on a polyclonal rabbit anti-saxitoxin antibody and compared with a conventional ELISA of saxitoxin using saxitoxin-bovine serum albumin conjugate as the coating antigen. In the experiments investigating cross-reactivities of various saxitoxin derivatives based on a competitive assay, significantly greater sensitivity was achieved with the novel approach, e.g. 35 pM saxitoxin could be detected (3 x 10(4) times lower concentrations than using the conjugate). The assay works well with mussel tissue homogenates, and because it does not require the use of the covalent saxitoxin-carrier conjugates it offers a simpler alternative to the traditional ELISA for saxitoxin.
Assuntos
Anticorpos/análise , Ensaio de Imunoadsorção Enzimática/métodos , Saxitoxina/análise , Anticorpos/imunologia , Concentração de Íons de Hidrogênio , Saxitoxina/imunologiaRESUMO
A competitive direct enzyme-linked immunofiltration assay for the detection of saxitoxin was developed, using polyclonal antibodies against saxitoxin and a saxitoxin-horseradish peroxidase conjugate. The test was performed in an eight-well plastic test device, in which antibody-coated nylon membranes were pressed tightly to an absorbent cellulose layer. Saxitoxin standard or sample extract solution, saxitoxin-conjugate, and enzyme substrate/chromogen solution were sequentially added on to the membrane. The test was evaluated visually by comparing the intensity of the resulting coloured (blue) dot with that of a negative control. The detection limits for saxitoxin in buffer solution and in shellfish tissue were 4 ng/ml and 80 ng/g respectively, with an assay time of less than 15 min. Under the conditions of the immunofiltration assay, decarbamoyl-saxitoxin, gonyautoxin 2/3, and neosaxitoxin standards (in buffer) gave a positive response at concentrations of about 10 ng/ml, 40 ng/ml, and 80 ng/ml, respectively. The relative cross-reactivity of the antibody to these PSPs was similar when determined using both direct and indirect (using a saxitoxin-bovine serum albumin conjugate) competitive enzyme immunoassays in microtitre plate format. In competitive direct microtitre plate assays, the 50% binding values found for saxitoxin, decarbamoyl-saxitoxin, gonyautoxin 2/3 and neosaxitoxin were 15 pg/ml, 47.5 pg/ml, 163.5 pg/ml, and 510 pg/ml respectively. In competitive indirect microtitre assay, the respective values were 138 pg/ml, 404 pg/ml, 1582 pg/ml, and 6982 pg/ml.
Assuntos
Bivalves/química , Técnicas Imunoenzimáticas , Saxitoxina/isolamento & purificação , Frutos do Mar , Animais , Toxinas Marinhas/isolamento & purificaçãoRESUMO
Four major paralytic shellfish poisoning (PSP) toxins in pure form-saxitoxin (STX), neosaxitoxin (NEO), gonyautoxin II (GTX II), and decarbamoylsaxitoxin (dcSTX), and mixed gonyautoxin II plus III (GTX II/III)-were toxicologically evaluated in the neuroblastoma cell bioassay and compared with the United States Food and Drug Administration standard reference saxitoxin (FDA STX). Mean relative toxicities of these experimental preparations were 99.5 (STX), 128.0 (NEO), 48.4 (GTX II) and 42.6 (dcSTX) when compared with FDA STX set arbitrarily at 100. Two mixtures of GTX II/III with relative concentrations of 1:2.5 and 4:1 had percentage relative toxicities of 52.4 and 54.5, respectively. Except for GTX II/III, which increased in toxicity, the toxicities of the experimental toxin preparations were not affected by boiling for 5 min in equal volumes of either 0.1 or 1.0 N HCl. Detailed examinations of dose-responses of each individual toxin preparation in the cell bioassay revealed some differences in response profiles. The toxicological data presented here support and extend previous information collected with the mouse bioassay and HPLC. The EC(50) values of STX and GTX II/III obtained under different conditions of matrix (acetic acid nu. ethanol), filtration (filtered 0.22 mum or unfiltered) and time in storage at 5 degrees C after opening of the sealed ampoules were largely unaffected by any of the experimental treatments.
RESUMO
A sample stacking procedure is presented for the capillary electrophoretic (CE) separation of paralytic shellfish poisoning (PSP) toxins dissolved in high ionic strength buffers. The application of such a stacking procedure prior to the zone electrophoretic separation is demonstrated for the analysis of decarbamoyl toxins arising from the digestion of PSP toxins by an hydrolytic enzyme from little neck clams (Protothaca staminea). Improvements in separation efficiency facilitated identification and quantitation of substrates and enzymatic products present in the digest using CE. The separation conditions developed were found to be entirely compatible with electrospray mass spectrometry, which permitted the analysis of PSP toxins and their decarbamoyl derivatives present in the low micromolar range in crude enzyme digests. The products released during the enzymatic digestion were identified using CE combined with tandem mass spectrometry.
Assuntos
Bivalves/química , Hidrolases de Éster Carboxílico/metabolismo , Eletroforese/métodos , Venenos de Moluscos/metabolismo , Animais , Espectrometria de Massas , Espectrofotometria UltravioletaRESUMO
Paralytic shellfish poisoning (PSP) is caused by mixtures of saxitoxin analogs of which more than eighteen are known. Reliable and sensitive analytical methods and assays for these toxins are essential to protect the consumer and the shellfish industry, but research has been restricted by a shortage of the pure compounds. Only saxitoxin has so far been generally available as a PSP toxin standard, yet sulfated analogs usually occur in higher concentrations than saxitoxin in toxic marine algae and shellfish. Methods are described for the purification of some of the common PSP toxins, in quantities sufficient for the preparation of PSP standards from the dinoflagellate Alexandrium excavatum, the giant sea scallop (Placopecten megallanicus) hepatopancreas, and the cyanobacterium Aphanizomenon flos-aquae. Purity was monitored by high performance liquid chromatography with fluorescence detection (HPLC-FD), ionspray mass spectrometry (ISP-MS), capillary electrophoresis (CE), and proton NMR spectroscopy.
Assuntos
Toxinas Marinhas/isolamento & purificação , Saxitoxina/toxicidade , Frutos do Mar , Animais , Cromatografia Líquida de Alta Pressão , Dinoflagellida , Eletroforese em Papel , Espectroscopia de Ressonância Magnética , Toxinas Marinhas/toxicidade , Padrões de Referência , Saxitoxina/análogos & derivados , Saxitoxina/isolamento & purificaçãoRESUMO
The distribution of kainic acid among various red algae was investigated. Analysis of free amino acids from different populations of Palmaria palmata showed that some were unable to accumulate kainic acid to detectable concentrations, whereas in two dwarf mutants it was a major component of the free amino acid composition. The amino acid profiles were also examined for unknown amino acids in the search for possible intermediates in kainic acid biosynthesis. The only unknown amino acid present in P. palmata extracts was isolated and identified by NMR spectroscopy as 1'-hydroxykainic acid. This compound was found in all samples that contained kainic acid. To investigate the effect of growth conditions on kainic acid production different strains of P. palmata were grown at 5, 10, and 15 degrees C with or without added nitrate. No effect on production was observed, suggesting that the growth conditions in these experiments do not affect the level of gene expression in the pathway of kainic acid biosynthesis. Furthermore, changing the growth conditions did not induce synthesis of kainic acid in the non-producing strains of Palmariales.
Assuntos
Ácido Caínico/análogos & derivados , Ácido Caínico/metabolismo , Nitratos/química , Rodófitas/metabolismo , Aminoácidos/análise , Fracionamento Químico , Cromatografia por Troca Iônica , Meios de Cultura , Ácido Caínico/química , Ácido Caínico/isolamento & purificação , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Mutação/genética , TemperaturaRESUMO
Ionspray mass spectrometry has been used to monitor the purification of saxitoxin, the parent compound in the family of toxins responsible for paralytic shellfish poisoning (PSP), from a strain of the dinoflagellate Alexandrium excavatum. Quantitative results obtained by flow-injection analysis are compared to those obtained by high-performance liquid chromatography with post-column oxidation and fluorescence detection. The coupling of liquid chromatography and capillary electrophoresis with ionspray mass spectrometry is described for the separation of mixtures of PSP toxins and the highly potent pufferfish toxin tetrodotoxin. Tandem mass spectrometry is used to provide the structural information, and the ability to distinguish isomeric PSP toxins both chromatographically and mass spectrometrically is demonstrated.
Assuntos
Espectrometria de Massas/métodos , Saxitoxina/química , Cromatografia Líquida de Alta Pressão/métodos , Eletroforese/métodos , Análise de Injeção de Fluxo , Espectrometria de FluorescênciaRESUMO
Three clones were isolated from a lobster digestive gland cDNA library, using oligonucleotide probes based on the partial amino terminal sequence of a digestive cysteine proteinase. The cDNAs, LCP1, LCP2 and LCP3 encode preproenzymes of 322, 323 and 321 amino acid residues, and putative mature enzymes of 217, 216 and 215 residues, respectively. Calculated mature protein molecular masses are 23386 (LCP1), 29093 (LCP2) and 23255 (LCP3) Sequence alignments show that the lobster enzymes are more similar to L (55-62% identity) than H (42-44%) or B (22-24%) cathepsins. Southern analysis indicated as many as eleven genes related to the three cDNAs.
Assuntos
Cisteína Endopeptidases/genética , DNA/genética , Sistema Digestório/enzimologia , Nephropidae/enzimologia , Animais , Sequência de Bases , Southern Blotting , Clonagem Molecular , Dados de Sequência Molecular , Mapeamento por Restrição , Alinhamento de Sequência , Homologia de Sequência do Ácido NucleicoRESUMO
A capillary electrophoresis (CE) method with UV detection is described for the separation and determination of underivatized toxins associated with paralytic shellfish poisoning (PSP). Confirmation of the electrophoretic peaks was facilitated by mass spectrometric (MS) detection using an ionspray CE-MS interface and by high-performance liquid chromatography with fluorescence detection. The determination of PSP toxins, such as saxitoxin and neosaxitoxin, in toxic dinoflagellates and scallops is demonstrated and comparisons are made with existing techniques.
Assuntos
Eletroforese/métodos , Toxinas Marinhas/análise , Frutos do Mar , Animais , Ação Capilar , Cianobactérias/análise , Dinoflagellida/análise , Fígado/química , Moluscos/análise , Saxitoxina/análogos & derivados , Saxitoxina/análiseRESUMO
A new cysteine proteinase was isolated from the digestive juice of the American lobster (Homarus americanus). The enzyme was purified by a combination of affinity and ion-exchange chromatography and gel filtration. The cysteine proteinase accounted for 80% of the proteolytic activity in the lumen of the hepatopancreas. The most potent heavy-metal inhibitors were Hg, Cu, and Ag ions. Inhibition by organic proteinase inhibitors, including E-64 [L-trans-epoxysuccinyl-leucylamido-(4-guanidino)butane] and activation of the enzyme by 2-mercaptoethanol and dithiothreitol are characteristic of cysteine proteinases. Several similarities to papain are noted and include the N-terminal sequence, of which 22 of the first 28 amino acids are identical. Some notable differences are the higher Mr of 28,000 compared with 23,350 for papain, and the low isoelectric point (pI 4.5) of the lobster enzyme. The effects of pH and temperature on catalytic activity of the lobster proteinase were studied with benzyloxycarbonylalanine p-nitrophenyl ester as the substrate. The kcat./Km value was effectively temperature-independent between 10 and 60 degrees C. The pH-activity profile for the lobster enzyme revealed four apparent protonation states, of which only two are active.
Assuntos
Cisteína Endopeptidases/isolamento & purificação , Nephropidae/enzimologia , Sequência de Aminoácidos , Aminoácidos/análise , Animais , Cromatografia , Cisteína Endopeptidases/metabolismo , Inibidores de Cisteína Proteinase , Estabilidade de Medicamentos , Liofilização , Suco Gástrico/enzimologia , Concentração de Íons de Hidrogênio , Hidrólise , Cinética , Metais/farmacologia , Dados de Sequência Molecular , Inibidores de Proteases/farmacologia , Homologia de Sequência do Ácido Nucleico , Especificidade por Substrato , TermodinâmicaRESUMO
Toxicity (extreme weakness, body temperature drop, cyanosis, some slow deaths) in test mice, upon intraperitoneal injection of standard-method paralytic shellfish poison (PSP) extracts of some PSP-free oysters, is consistent with the relatively high levels of zinc in these extracts. As a rough guideline, the threshold for a toxic response corresponds to a drained tissue zinc level of over 900 micrograms/g. The identification of zinc as the substance responsible has been supported by inducing toxicity in control extracts by spiking with nontoxic levels of zinc, and by eliminating toxicity from toxic extracts by chemical removal (precipitation, ion exchange) of metals.
Assuntos
Contaminação de Alimentos/análise , Ostreidae/análise , Zinco/intoxicação , Animais , Bioensaio , CamundongosRESUMO
Gigartinine, 5-(3-amidinoureido)-2-aminovaleric acid, and L-citrullinyl-L-arginine were islated from aqueous extracts of Chondrus crispus (Rhodophyceae). Their identifications were confirmed by chemical procedures, and 1H and 13C nuclear magnetic resonance and infrared spectroscopic methods. Citrullinylarginine, gigartinine, taurine, citrulline, and glutamic acid were the predominant free amino compounds. Citrullinylarginine showed the most pronounced change in concentration. This occurred during the winter months when it reached a maximum (in March) of 58 mumol/g fresh weight, a value 10 times greater than that of any of the free amino acids and equal to 50% of the total organic nitrogen in the plant; All of these compounds were depleted to a minimum of about 1 mumol/g fresh weight in October. Both gigartinine and citrullinylarginine were detected in Ahnfeltia plicata, Gracilaria sp. Petrocelis middendorfii, Polyides rotundus, Polysiphonia lanosa, and Rhodomela confervoides.
Assuntos
Aminoácidos/metabolismo , Dipeptídeos/metabolismo , Rodófitas/metabolismo , Ureia/análogos & derivados , Arginina/análise , Citrulina/análise , Dipeptídeos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Ornitina/análise , Estações do Ano , Ureia/metabolismoRESUMO
Cytochrome f was isolated from the brown alga Alaria esculenta and the amino acid sequence was determined. The native haemoprotein has a molecular weight of 9800 and consists of a single polypeptide chain of 86 amino acid residues with a haem group bonded to cysteine residues at positions 14 and 17. The N-terminus is not acetylated and no methylated lysines were found. Sequences of three other algal cytochromes f were compared with that of Alaria and 22 out of 92 positions were common to the four sequences. One-half of these conserved sites occur between positions 49 and 63. Detailed evidence for the amino acid sequence of Alaria cytochrome has been deposited as Supplementary Publication SUP 50048 (6 pages) at the British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1975) 145, 5.
Assuntos
Citocromos , Eucariotos/análise , Phaeophyceae/análise , Sequência de Aminoácidos , Citocromos/análise , Dipeptidases , Heme/análise , Peso Molecular , Fragmentos de Peptídeos/análise , Especificidade da Espécie , TripsinaAssuntos
Aminoácidos/análise , Grupo dos Citocromos c/análise , Eucariotos/análise , Sequência de Aminoácidos , Animais , Asparagina/análise , Ácido Aspártico/análise , Autoanálise , Bactérias/análise , Quimotripsina , Cisteína/análise , Compostos de Dansil , Transporte de Elétrons , Glicina/análise , Cavalos , Hidrólise , Mitocôndrias Musculares/análise , Pepsina A , Peptídeos/análise , Fotossíntese , Especificidade da Espécie , Tiocianatos , Fatores de Tempo , TripsinaAssuntos
Citocromos , Eucariotos/análise , Aminoácidos/análise , Arginina/análise , Autoanálise , Precipitação Química , Cromatografia em Gel , Cromatografia por Troca Iônica , Cisteína/análise , Citocromos/análise , Citocromos/isolamento & purificação , Diálise , Transporte de Elétrons , Eletroforese Descontínua , Eucariotos/crescimento & desenvolvimento , Liofilização , Heme/análise , Histidina/análise , Ferro/análise , Focalização Isoelétrica , Metionina/análise , Métodos , Peso Molecular , Oxirredução , Fotossíntese , Prolina/análise , Compostos de Amônio Quaternário , Espectrofotometria , Sulfatos , Triptofano/análise , Ultracentrifugação , Raios UltravioletaRESUMO
The amino acid sequence of Phaseolus aureus L. (mung-bean) cytochrome c has been determined. The molecule consists of a single polypeptide chain of 111 amino acid residues and is homologous with other mitochondrial cytochromes c. Comparison with the amino acid sequence of wheat-germ cytochrome c (Stevens, Glazer & Smith, 1967) shows 14 differences. On alignment with mammalian cytochromes c, mung-bean cytochrome c has an N-acetylated ;tail' of eight amino acid residues similar to that found in wheat-germ cytochrome c. Of the 22 positions in wheat-germ cytochrome c that contain amino acid residues unique to these positions, 20 were found to contain the same ones in mung-bean cytochrome c. The in-N-trimethyl-lysine residues reported for wheat-germ cytochrome c (Delange, Glazer & Smith, 1969) in positions 72 and 86 were also found in these positions in mung-bean cytochrome c. The sequence was determined from 3mumol, by using chymotryptic and tryptic peptides which were analysed by the ;dansyl'-Edman method (Gray & Hartley, 1963a), with confirmation by amino acid analysis.