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Background: Diabetic retinopathy (DR) is one of the most common eye diseases. Convolutional neural networks (CNNs) have proven to be a powerful tool for learning DR features; however, accurate DR grading remains challenging due to the small lesions in optical coherence tomography angiography (OCTA) images and the small number of samples. Methods: In this article, we developed a novel deep-learning framework to achieve the fine-grained classification of DR; that is, the lightweight channel and spatial attention network (CSANet). Our CSANet comprises two modules: the baseline model, and the hybrid attention module (HAM) based on spatial attention and channel attention. The spatial attention module is used to mine small lesions and obtain a set of spatial position weights to address the problem of small lesions being ignored during the convolution process. The channel attention module uses a set of channel weights to focus on useful features and suppress irrelevant features. Results: The extensive experimental results for the OCTA-DR and diabetic retinopathy analysis challenge (DRAC) 2022 data sets showed that the CSANet achieved state-of-the-art DR grading results, showing the effectiveness of the proposed model. The CSANet had an accuracy rate of 97.41% for the OCTA-DR data set and 85.71% for the DRAC 2022 data set. Conclusions: Extensive experiments using the OCTA-DR and DRAC 2022 data sets showed that the proposed model effectively mitigated the problems of mutual confusion between DRs of different severity and small lesions being neglected in the convolution process, and thus improved the accuracy of DR classification.
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Global food production faces challenges in balancing the need for increased yields with environmental sustainability. This study presents a six-year field experiment in the North China Plain, demonstrating the benefits of diversifying traditional cereal monoculture (wheat-maize) with cash crops (sweet potato) and legumes (peanut and soybean). The diversified rotations increase equivalent yield by up to 38%, reduce N2O emissions by 39%, and improve the system's greenhouse gas balance by 88%. Furthermore, including legumes in crop rotations stimulates soil microbial activities, increases soil organic carbon stocks by 8%, and enhances soil health (indexed with the selected soil physiochemical and biological properties) by 45%. The large-scale adoption of diversified cropping systems in the North China Plain could increase cereal production by 32% when wheat-maize follows alternative crops in rotation and farmer income by 20% while benefiting the environment. This study provides an example of sustainable food production practices, emphasizing the significance of crop diversification for long-term agricultural resilience and soil health.
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Gases de Efeito Estufa , Solo , Solo/química , Gases de Efeito Estufa/análise , Carbono/análise , Óxido Nitroso/análise , Agricultura , Produtos Agrícolas , Grão Comestível/química , Verduras , Zea mays , Triticum , China , Produção AgrícolaRESUMO
PURPOSE: The optic disc and the macular are two major anatomical structures in the human eye. Optic discs are associated with the optic nerve. Macular mainly involves degeneration and impaired function of the macular region. Reliable optic disc and macular segmentation are necessary for the automated screening of retinal diseases. METHODS: A swept-source OCTA system was designed to capture OCTA images of human eyes. To address these segmentation tasks, first, we constructed a new Optic Disc and Macula in fundus Image with optical coherence tomography angiography (OCTA) dataset (ODMI). Second, we proposed a Coarse and Fine Attention-Based Network (CFANet). RESULTS: The five metrics of our methods on ODMI are 98.91 % , 98.47 % , 89.77 % , 98.49 % , and 89.77 % , respectively. CONCLUSIONS: Experimental results show that our CFANet has achieved good performance on segmentation for the optic disc and macula in OCTA.
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Aprendizado Profundo , Oftalmologia , Humanos , Vasos Retinianos/diagnóstico por imagem , Angiofluoresceinografia/métodos , Tomografia de Coerência Óptica/métodosRESUMO
BACKGROUND: Biochar addition strongly alters net carbon (C) balance in agroecosystems. However, the effects of biochar addition on net C balance of maize field under various irrigation water quantities and qualities remains unclear. Thus, a field experiment combining two irrigation levels of full (W1) and deficit irrigation (W2 = 1/2 W1), two water salinity levels of fresh (S0, 0.71 g L-1 ) and brackish water (S1, 4 g L-1 ), and two biochar addition rates of 0 t ha-1 (B0) and 60 t ha-1 (B1) was conducted to investigate soil carbon dioxide (CO2 ) emissions, maize C sequestration and C budget. RESULTS: Compared with W1, W2 reduced average cumulative CO2 emissions by 6.5% and 19.9% for 2020 and 2021, respectively. The average cumulative CO2 emissions under W1S1 treatments were 5.4% and 22.3% lower than W1S0 for 2020 and 2021, respectively, whereas W2S0 and W2S1 had similar cumulative CO2 emissions in both years. Biochar addition significantly increased cumulative CO2 emissions by 17.8-23.5% for all water and salt treatments in 2020, and reduced average cumulative CO2 emissions by 11.9% for W1 but enhanced it by 8.0% for W2 in 2021. Except for W2S1, biochar addition effectively increased total maize C sequestration by 6.9-14.8% for the other three treatments through ameliorating water and salt stress over the 2 years. Compared with W1S0, W1S1 did not affect net C sequestration, but W2 treatments significantly decreased it. Biochar addition increased net C sequestration by 39.47-43.65 t C ha-1 for four water and salt treatments for the 2 years. CONCLUSION: These findings demonstrate that biochar addition is an effective strategy to increase both crop C sequestration and soil C storage under suitable water-saving irrigation methods in arid regions with limited freshwater resources. © 2023 Society of Chemical Industry.
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Solo , Zea mays , Dióxido de Carbono/análise , Carvão Vegetal , ÁguaRESUMO
BACKGROUND: Increasing crop yield per unit area by increasing planting density is essential to ensure food security. However, the optimal combination of planting density and nitrogen (N) application for high-yielding maize and its source-sink characteristics need to be more clearly understood. RESULTS: A 2-year field experiment was conducted combining three planting densities (D1: 70 000 plants ha-1 ; D2: 100 000 plants ha-1 ; D3: 130 000 plants ha-1 ) and three nitrogen rates (N1: 150 kg hm-2 ; N2: 350 kg hm-2 ; N3: 450 kg hm-2 ). The results showed that increasing planting density significantly increased leaf area index and grain yield but negatively affected ear traits. The Richards model was used to fit the dynamic changes of dry matter accumulation of maize under different treatments, and the fitting results were good. Increasing planting density increased population yield while limiting the development of individual plants, bringing the period of rapid dry matter accumulation to an early end and accelerating leaf senescence. An appropriate nitrogen rate could prolong the period of rapid accumulation of dry matter in maize, and increase the 100-kernel weight. Increasing planting density enhanced post-silking dry matter accumulation to a lesser extent, and the source-sink relationship of the maize population gradually developed from sink limitation to source limitation with increasing planting density. CONCLUSION: The decrease in yield due to the insufficient source strength to meet the sink demand at too high densities was the reason that limited further improvement of the optimal planting density. An appropriate nitrogen rate facilitated the realization of yield potential at high density. © 2023 Society of Chemical Industry.
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Nitrogênio , Zea mays , Biomassa , Grão Comestível , ChinaRESUMO
Agricultural ecosystem water use efficiency (WUE) is an important indicator reflecting carbon-water coupling, but its control mechanisms in managed fields remain unclear. In order to reveal the influencing factors of WUE in the agricultural field under mulched drip irrigation (DM), we carried out the 8-year continuous observations in a maize field from Northwestern China. The structural equation model, relative importance analysis and principal component analysis were used to quantify the regulation effects of environmental and biological factors on WUE at different time scales, in different growth stages and under different hydrothermal conditions. The results showed that annual WUE varied between 2.18 g C Kg-1 H2O and 3.60 g C Kg-1 H2O, with a multi-year mean of 2.91 g C Kg-1 H2O. The total effects of air temperature on the daily WUE in the whole growth period, the vegetative growth stage, the warm and dry years, the cold and wet years, and the warm and wet years were the largest, with values of 0.61, 0.80, 0.70, 0.70 and 0.91 respectively. However, vapor pressure deficit and net radiation had the largest total effect in the cold and dry years (-0.63) and the reproductive growth stage (-0.49), respectively. Leaf biomass played a leading role in regulating the daily and interannual WUE, and the relative importance of leaf biomass to WUE in the vegetative growth stage was up to 75 %. In the warm and wet years, the relative importance of root biomass to WUE was 33 %, slightly higher than that of leaf biomass (31 %). At the same time, we found that Ta has the potential to increase WUE under future climate warming. Our results improve the understanding of carbon-water coupling mechanisms and provide important enlightenment on how crop ecosystems should adapt to future climate change.
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Água , Zea mays , Ecossistema , Biomassa , Carbono , Irrigação Agrícola/métodosRESUMO
This study is aimed at assessing the current status of ACS patients' health literacy and medication compliance, analyzing the relationship between the two, and providing ideas for clinically improving the medication compliance of ACS patients and preventing the recurrence of cardiovascular events. ACS patients need long-term medication to prevent vascular restenosis after surgery, and bad living habits and mood swings will affect postoperative recovery, so clinical interventions are needed to help patients establish a healthy lifestyle. The effect of conventional care is not ideal. Therefore, this paper uses regression analysis to analyze the correlation between the health literacy status of ACS patients and the compliance behavior, combines the investigation and experiment to perform regression analysis and uses mathematical statistics to process data. The connection between health literacy level and compliance behavior is discovered via a study, providing a point of reference for future research.
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Síndrome Coronariana Aguda/tratamento farmacológico , Síndrome Coronariana Aguda/psicologia , Letramento em Saúde , Adesão à Medicação , Idoso , China , Biologia Computacional , Feminino , Letramento em Saúde/estatística & dados numéricos , Humanos , Masculino , Adesão à Medicação/estatística & dados numéricos , Pessoa de Meia-Idade , Conhecimento do Paciente sobre a Medicação/estatística & dados numéricos , Análise de RegressãoRESUMO
BACKGROUND: Under the escalating threat to sustainable development from the global increase in carbon dioxide concentrations, the variations in carbon flux in the farmland ecosystem and their influencing factors have attracted global attention. Over the past few decades, with the development of eddy covariance technology, the carbon fluxes of farmlands have been determined in many countries. However, studies are very limited for drip irrigation maize the arid regions in northwestern China, which covers a large area where a mixed mode of agriculture and grazing is practiced. RESULTS: To study the effects of drip irrigation on the net ecosystem productivity (NEE), ecosystem respiration (ER), gross primary production (GPP) and net biome productivity (NBP) in the arid regions of northwestern China, we measured the carbon flux annually from 2014 to 2018 using an eddy covariance system. Our results showed that the maize field carbon flux exhibited single-peak seasonal patterns during the growing seasons. During 2014-2018, the NEE, ER and GPP of the drip-irrigated maize field ranged between - 407 ~ - 729 g C m-2, 485.46 ~ 975.46 g C m-2, and 1068.23 ~ 1705.30 g C m-2. In four of the 5 study years, the ER released back to the atmosphere was just over half of the carbon fixed by photosynthesis. The mean daily NEE, ER and GPP were significantly correlated with the net radiation (Rn), air temperature (Ta), leaf area index (LAI) and soil moisture (SWC). The results of path analysis showed that leaf area index is the main driving force of seasonal variation of carbon flux. When harvested removals were considered, the annual NBP was - 234 g C m-2, and the drip-irrigated maize field was a carbon source. CONCLUSIONS: This study shows the variation and influencing factors of NEE, ER and GPP in the growth period of spring maize under film drip irrigation in arid areas of northwest China. The ecosystem was a carbon sink before maize harvest, but it was converted into a carbon source considering the carbon emissions after harvest. The variation of carbon flux was influenced by both environmental and vegetation factors, and its leaf area index was the main driver that affects the seasonal variation of carbon flux.
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BACKGROUND: Keto-analogues administration plays an important role in clinical chronic kidney disease (CKD) adjunctive therapy, however previous studies on their reno-protective effect mainly focused on kidney pathological changes induced by nephrectomy. This study was designed to explore the currently understudied alternative mechanisms by which compound α-ketoacid tablets (KA) influenced ischemia-reperfusion (IR) induced murine renal injury, and to probe the current status of KA administration on staving CKD progression in Chinese CKD patients at different stages. METHODS: In animal experiment, IR surgery was performed to mimic progressive chronic kidney injury, while KA was administrated orally. For clinical research, a retrospective cohort study was conducted to delineate the usage and effects of KA on attenuating CKD exacerbation. End-point CKD event was defined as 50% reduction of initial estimated glomerular filtration rate (eGFR). Kaplan-Meier analysis and COX proportional hazard regression model were adopted to calculate the cumulative probability to reach the end-point and hazard ratio of renal function deterioration. RESULTS: In animal study, KA presented a protective effect on IR induced renal injury and fibrosis by attenuating inflammatory infiltration and apoptosis via inhibition of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. In clinical research, after adjusting basic demographic factors, patients at stages 4 and 5 in KA group presented a much delayed and slower incidence of eGFR decrease compared to those in No-KA group (hazard ratio (HR) = 0.115, 95% confidence interval (CI) 0.021-0.639, p = 0.0134), demonstrating a positive effect of KA on staving CKD progression. CONCLUSION: KA improved IR induced chronic renal injury and fibrosis, and seemed to be a prospective protective factor in end stage renal disease.
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Suplementos Nutricionais , Progressão da Doença , Cetoácidos/uso terapêutico , Sistema de Sinalização das MAP Quinases , NF-kappa B/metabolismo , Insuficiência Renal Crônica/tratamento farmacológico , Insuficiência Renal Crônica/patologia , Animais , Apoptose/efeitos dos fármacos , Dieta com Restrição de Proteínas , Feminino , Humanos , Inflamação/patologia , Cetoácidos/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Masculino , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , Probabilidade , Insuficiência Renal Crônica/etiologia , Insuficiência Renal Crônica/fisiopatologia , Traumatismo por Reperfusão/complicações , Análise de Sobrevida , ComprimidosRESUMO
BACKGROUND: Putative endothelial progenitor cells (pEPCs) have been confirmed to participate in alleviation of renal fibrosis in several ischaemic diseases. However, their mechanistic effect on renal fibrosis, which is characterized by vascular regression and further rarefaction-related pathology, remains unknown. METHODS: To explore the effect and molecular mechanisms by which pEPCs act on unilateral ureteral obstruction (UUO)-induced renal fibrosis, we isolated pEPCs from murine bone marrow. In vivo, pEPCs (2 × 105 cells/day) and pEPC-MVs (microvesicles) were injected into UUO mice via the tail vein. In vitro, pEPCs were co-cultured with renal-derived pericytes. Pericyte-myofibroblast transition was evaluated using the myofibroblast marker α-smooth muscle actin (α-SMA) and pericyte marker platelet-derived growth factor receptor ß (PDGFR-ß). RESULTS: Exogenous supply of bone marrow-derived pEPCs attenuated renal fibrosis by decreasing pericyte-myofibroblast transition without significant vascular repair in the UUO model. Our results indicated that pEPCs regulated pericytes and their transition into myofibroblasts via pEPC-MVs. Co-culture of pericytes with pEPCs in vitro suggested that pEPCs inhibit transforming growth factor-ß (TGF-ß)-induced pericyte-myofibroblast transition via a paracrine pathway. CONCLUSION: pEPCs effectively attenuated UUO-induced renal fibrosis by inhibiting pericyte-myofibroblast transition via a paracrine pathway, without promoting vascular repair.
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Células da Medula Óssea , Células Progenitoras Endoteliais , Miofibroblastos , Comunicação Parácrina , Pericitos , Obstrução Ureteral , Animais , Células da Medula Óssea/metabolismo , Células da Medula Óssea/patologia , Células Progenitoras Endoteliais/metabolismo , Células Progenitoras Endoteliais/patologia , Células Progenitoras Endoteliais/transplante , Fibrose , Nefropatias/etiologia , Nefropatias/metabolismo , Nefropatias/patologia , Nefropatias/terapia , Masculino , Camundongos , Camundongos Transgênicos , Miofibroblastos/metabolismo , Miofibroblastos/patologia , Pericitos/metabolismo , Pericitos/patologia , Obstrução Ureteral/complicações , Obstrução Ureteral/metabolismo , Obstrução Ureteral/patologia , Obstrução Ureteral/terapiaRESUMO
Quantifying the influence of driving factors on irrigation water productivity (IWP) is vital for efficient agricultural water use. This study analyzed contributions of agronomic practice and climatic factors to the changes of IWP, based on the data from 1981 to 2012 in Hexi Corridor, Northwest China. Cobb-Douglas production functions were developed by the partial least squares method and contribution rates of the driving factors were calculated. Results showed that IWP and its driving factors increased during the study period, with different changing patterns. IWP was significantly correlated with the agronomic practice factors, daily mean temperature and solar radiation of the crop growing period. The agronomic practice factors including irrigation, fertilization, agricultural film, and agricultural pesticide contributed 20.6%, 32.8%, 42.3% and 11.1% respectively to the increase of IWP; and the contribution rates of the climatic factors, i.e. daily mean temperature and solar radiation, are -0.9% and 0.9%. And the contributions of these factors changed in different sub-periods. It is concluded that agronomic practice factors influenced IWP much more than climatic factors. The improvement of IWP should rely on advanced water-saving technology and application of optimum (need-based) fertilizer, agricultural film and pesticide, ensuring efficient use of agronomic inputs in the study area.
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This paper studied the stem sap flow of grape in arid oasis region of Shiyang River basin under conventional drip irrigation (CDI), alternate drip irrigation (ADI), and fixed drip irrigation (FDI), and its relationships with meteorological conditions and soil moisture content. The results showed that the stem sap flow of grape had an obvious day-night rhythm synchronous with solar radiation, and was significantly higher under CDI than under ADI and FDI during new branch growth and flowering stages. Solar radiation and air temperature were the main meteorological factors affecting the hourly sap flow, and the daily stem sap flow had linear relationships with daily air temperature and wind speed. The correlation coefficients between the stem sap flow and the meteorological factors ranked in the order of CDI > ADI > FDI. There was a significant correlation between daily stem sap flow and reference crop evapotranspiration (ET0). Compared with CDI, ADI could save 50% of irrigation water while the stem sap flow only reduced by 6.56%, and an obvious compensation effect between stem sap flow and hydraulic conductivity was observed.