RESUMO
One new norsesterterpene peroxide, rhopaloic acid H (1), along with two known related metabolites 2 and 3, were isolated from a marine sponge Hippospongia sp. The structures of compounds were elucidated by means of IR, MS, and NMR techniques and comparison of the NMR data with those of known analogues. Evaluation of the cytotoxicities revealed that compound 2 exhibited significant cytotoxicity against DLD-1, Molt 4, T47D and K-562 cell lines, with IC50 values of 3.18, 0.69, 2.22 and 1.06 µg/mL, respectively. Moreover, compound 3 also showed significant K562 inhibitory activity, with IC50 value of 3.65 µg/mL.
Assuntos
Peróxidos/isolamento & purificação , Poríferos/química , Sesterterpenos/isolamento & purificação , Animais , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Biologia Marinha , Peróxidos/química , Sesterterpenos/químicaRESUMO
Chemical investigation of a Formosan sponge Axinyssa sp. has led to the isolation of five nitrogenous bisabolene-type sesquiterpenes 1-5, including two new compounds axinysalines A (1) and B (2). The structures of new compounds were elucidated by analysis of high resolution (HR)-MS and two dimensional (2D)-NMR spectra and comparison of its NMR data with those of known analogues. Compound 1 exhibited moderate to weak cytotoxicity against Molt 4 and K562 cancer cell lines.
Assuntos
Antineoplásicos/química , Poríferos/química , Sesquiterpenos/química , Animais , Antineoplásicos/farmacologia , Linhagem Celular Tumoral/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Células K562/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Sesquiterpenos/isolamento & purificação , Sesquiterpenos/farmacologiaRESUMO
One new 5alpha,8alpha-epidioxysterol, 3-acetylaxinysterol (1), along with one known sterol, axinysterol (2), were isolated from a Formosan sponge, Axinyssa sp.. The structures of the compounds were determined by spectroscopic methods and the absolute configuration of 2 was further confirmed by single-crystal X-ray diffraction analysis for the first time. Compound 2 exhibited significant cytotoxicity against K562 and Molt 4 cancer cell lines.
