[Quality evaluation method of Lepidium meyenii using UPLC-UV-Q-TOF-MS].

In order to evaluate the quality of different varieties of Maca(Lepidium meyenii), the main chemical components in Maca were investigated and a method for simultaneous determination of the main chemical components in Maca was established. UPLC-UV-Q-TOF-MS technology and reference materials were used to identify the structures of 19 main components in Maca. Seven compounds with UV absorption and high contents were selected to establish a simultaneous concentration determination method. The method was employed with a Waters Acquity I-Class~(TM) liquid chromatographic system coupled with a PDA detector and a Waters Acquity Cortecs C_(18)~+ column(2.1 mm×100 mm, 1.6 µm), and acetonitrile-0.2% phosphoric acid water was used as mobile phase(0.45 mL·min~(-1)). The detection wavelength was 195 nm and the column temperature was maintained at 40 ℃. There was efficient separation of seven compounds, p-hydroxybenzylglucosinolate, benzylglucosinolate, N-benzyl-9Z,12Z,15Z-octadecatrienamid, N-benzyl-9Z,12Z-octadecadienamide, N-(3-methoxybenzyl)-hexadecanamide, N-benzyl-hexadecanamide, and N-benzyl-9Z-octadecenamide. The stan-dard calibration curves were good(R~2>0.999). The precision, stability and repeatability were also good. The linearity ranges were 0.197-4.980 µg·mL~(-1) to 193.67-796.8 µg·mL~(-1), and the average recovery rate was 96.71%-103.9%. The average concentration of glucosinolates and macamides in Maca were 1.20% and 0.20%, respectively. Among four kinds of Maca grown in China, the concentration of glucosinolates in yellow Maca and black Maca were relatively high(1.55%), followed by white Maca(0.93%), and purple Maca(0.76%). The concentration of macamides in yellow, purple and white Maca was similar(0.23%-0.29%), however black Maca had significantly lower concentration(0.15%). Peru Maca tested in this study had the lowest concentration of these compounds. This qua-lity evaluation method was fast, accurate, and comprehensively reflects the concentration of the main chemical components in Maca, which provides a useful reference for the quality control and evaluation of Maca.

[Research progress on chemical constituents from Artemisiae Argyi Folium and their pharmacological activities and quality control].

Artemisiae Argyi Folium is a traditional medicine commonly used in traditional Chinese medicine(TCM). It contains volatile oil, flavones, phenylpropanoids, terpenes and other chemical constituents. It has the functions of antibacterial, antiviral, hemostasis, anti-tumor, liver protection, analgesic, anti-inflammatory, anti-oxidation, relieving cough and asthma and other pharmacological activities. At present, many useful researches on the quality of moxa floss and Artemisiae Argyi Folium have been carried out on the contents of volatile oil, flavones, phenylpropanoids, the storage time of Artemisiae Argyi Folium, the processing of moxa, the genuineness of Artemisiae Argyi Folium, and their heat release properties in combustion. This paper summarized the literature on the chemical composition, pharmacological activities and quality control of Artemisiae Argyi Folium and provided the basis for the further development and utilization of Artemisiae Argyi Folium.