Minimizing pain in deer antler removal: Local anaesthetics in ZnO nanoparticle based collagen dressings as a promising solution.

Antler removal in deer is a common practice for various purposes, including meat production and traditional medicine. However, the current industry practice using lidocaine as a local anesthetic has limitations, such as short duration of action and the potential for postoperative infections. In this study, we investigated the performance of a ZnO collagen nanocomposites loaded with local anesthetics to improve wound management and alleviate pain associated with antler removal in red deer. The research involved the preparation of collagen nanocomposites with local anesthetics and testing the drug release rates using in vitro drug release tests. Pharmacokinetic analysis was performed to evaluate the total drug release from the collagen matrix in red deer after velvet removal. Additionally, the analgesic efficacy of these collagen nanocomposite dressings was assessed after antler removal in red deer. Functionalized ZnO nanoparticles were incorporated into collagen fibers to enhance their mechanical stability and prolong drug release. The developed collagen nanocomposites aimed to slowly release local anesthetics and promote wound healing. The findings of this research could have significant implications for improving the pain management and wound healing associated with antler removal in deer. The results obtained from the in vitro drug release tests, pharmacokinetic analysis, and analgesic efficacy evaluations provide valuable insights into the understanding and development of novel approaches for antler removal procedures in red deer. The findings contribute to the advancement of knowledge in this field and lay the foundation for future implementation of improved techniques and protocols for antler removal.

Hydrogel dressings and their application in burn wound care.

After a burn injury, skin loses its protective properties, leaving the body open to a number of complications. The gold standard treatment for burn wounds is autologous skin grafting; however, this may not always be an option, due to insufficient amounts of donor skin. Wound dressings can be used as a primary treatment in those patients who are unsuitable for skin grafting. Many different materials are used to make the dressings; current materials include hydrocolloids, alginates, hydrogels, collagen and hyaluronic acid. There is currently no gold standard of burns dressing that is universally accepted. However, hydrogels have been shown to exhibit a great number of beneficial properties and are the most favourable for use in burns patients. We discuss the use of hydrogel dressings, including their properties and clinical application.

Synthesis and Properties of Two PRODAN-based Fluorescent Models of Cholesterol.

The syntheses and photophysical properties of 1-(5-methylhexyl)-2,3,7,8-tetrahydro-1H-naphtho[2,1-e]indol-9(6H)-one (7a) and 1-(5-methylhexyl)-2,3,8,9-tetrahydro-1H-naphtho[2,1-e]indol-6(7H)-one (7b) are reported. They are prepared in eight steps from the corresponding bromonaphthylamines. These fluorescent compounds have PRODAN-like cores, and they are structurally similar to cholesterol. Compound 7a is the first reported PRODAN derivative where both the amino and carbonyl groups are constrained to be coplanar with the naphthalene core. Comparing the photophysical behavior of these compounds with related compounds indicates that locking the amino group in a five-membered ring enhances their desirable properties as solvent polarity sensors.

Packed red blood cell-associated arginine depletion is mediated by arginase.

BACKGROUND: Packed red blood cells (PRBCs) transfusion is associated with immune suppression, but these mechanisms are incompletely understood. PRBCs contain arginase, an enzyme that converts arginine to ornithine, and is known to limit arginine availability and suppress cellular immunity. We sought to determine whether PRBC arginase causes arginine depletion, potentially contributing to immunosuppression. METHODS: A model of transfusion was designed by adding either centrifuged acellular supernatant from the PRBC unit (plasma) or total fluid from the unit (plasma+RBC [red blood cells]) to cell culture media. Through an institutional review board-approved protocol, PRBC units were isolated and processed by an accredited blood bank and stored at 4 degrees C. Leukoreduced PRBCs or supernatant aliquots were withdrawn every 5 days to 7 days for 42 days. Cell cultures were created with standard Roswell Park Memorial Institute media, controlling the arginine level at 80 micromol/L (approximating human serum), and adding 20% plasma or plasma+RBC. An irreversible arginase blocker (nor-N-omega-OH-L-arginine) was added to selected cultures. After 24 hours, culture arginase activity was measured by ornithine synthesis, and amino acid levels were measured using mass spectroscopy. RESULTS: Culture arginase activity was increased by both plasma and plasma+RBC, but with plasma+RBC this did not reach statistical significance. Arginine levels were decreased and ornithine levels increased in cultures containing either supernatant or PRBC, as compared with control media. Addition of nor-N-omega-OH-l-arginine significantly decreased cell culture arginase activity, restored arginine levels, and diminished ornithine synthesis. CONCLUSIONS: Arginase is present in PRBC units and causes arginine depletion. Depletion of arginine by PRBC arginase is a potential novel mechanism for immunosuppression.

Atypical etiology of massive gastrointestinal bleeding: arterio-enteric fistula following enteric drained pancreas transplant.

Pancreas transplantation is an established treatment for selected type I insulin-dependent diabetes mellitus (DM). Increasingly, enteric drainage of exocrine secretions has been performed in preference to bladder drainage. We present two cases of massive gastrointestinal hemorrhage (GIH) related to arterial-graft duodenal fistulas, a rare cause of massive bleeding. Case 1 DM is a 49-year-old male who underwent simultaneous kidney pancreas transplantation (SPK) for DM and end-stage renal disease (ESRD). He developed a transplant duodenal stump leak that resolved with drainage. He presented with massive hemorrhage at 2 months. Angiography revealed a fistula between the graft-recipient arterial anastomosis and the stump leak. This was managed by transplant pancreatectomy. Case 2 SB is a 37-year-old male who underwent pancreas-after-kidney transplantation (PAK) for type I DM. At 6 months, the pancreas graft failed due to chronic rejection. He presented 9 months later with massive hemorrhage. Upper and lower endoscopy were inconclusive. Angiography revealed a fistula between the transplant arterial graft and the transplant duodenum. This was initially managed by coil embolization and definitively by transplant pancreatectomy. Patients with functional or nonfunctional pancreas transplants presenting with massive GIH not readily localized by endoscopy should undergo angiography to exclude this unusual etiology.