Chronic inhalation of H2S in low concentration induces immunotoxicity and inflammatory effects in lung tissue of rats.

Hydrogen sulfide (H2S) is a typical odour compound mainly causing respiratory and central nervous system symptoms. However, the immunotoxicity of inhaled H2S and the underlying mechanisms remain largely unknown. In this study, a low-dose inhalation exposure to H2S was arranged to observe inflammatory response and immunotoxicity in lung tissue of rats. Low concentrations of H2S exposure affected the immune level of pulmonary tissue and peripheral blood. Significant pathological changes in lung tissue in the exposure group were observed. At low concentration, H2S not only induced the upregulation of AQP-4 and MMP-9 expression but also stimulated immune responses, initiating various anti-inflammatory and inflammatory factors, altering tissue homeostatic environments. The TNF and chemokine signaling pathway played an important role which can promote the deterioration of pulmonary inflammatory processes and lead to lung injury and fibrosis. Excessive immune response causes an inflammatory effect and blood-gas barrier damage. These data will be of value in evaluating future occupational health risks and providing technical support for the further development of reliable, sensitive, and easy-to-use screening indicators of exposure injury.

Sediment-water distribution and potential sources of polyhalogenated carbazoles in a coastal river locating at a north metropolis, China.

The fate and transformation of PHCZs in the coastal river environment are not yet comprehensively understood. Paired river water and surface sediment were collected, and 12 PHCZs were analyzed to find out their potential sources and investigate the distribution of PHCZs between river water and sediment. The concentration of ∑PHCZs varied from 8.66 to 42.97 ng/g (mean 22.46 ng/g) in sediment and 17.91 to 81.82 ng/L (mean 39.07 ng/L) in river water. 18-B-36-CCZ was the dominant PHCZ congener in sediment, while 36-CCZ was in water. Meanwhile, the logKoc values for CZ and PHCZs were among the first calculated in the estuary and the mean logKoc varied from 4.12 for 1-B-36-CCZ to 5.63 for 3-CCZ. The logKoc values of CCZs were higher than those of BCZs, this may suggest that sediments have a higher capacity for accumulation and storage of CCZs than highly mobile environmental media.

Occurrence and distribution of polyhalogenated carbazoles in eastern Tibetan Plateau soils along the slope of Mt. Qionglai.

Polyhalogenated carbazoles (PHCZs), are considered as potential persistent organic pollutants (POPs), which have been frequently detected in the environment. However, the altitudinal distribution characteristics and possible sources of PHCZs in high mountain soils are still unknown. The present study was the first to analyze PHCZs in soil samples collected along the eastern slope of Mt. Qionglai (MQ), the east edge of the Tibetan Plateau. The concentration of ΣPHCZs (based on dry weight) ranges from 14.4 to 107 ng/g (median value of 40.9), which was at high end of the range reported in soils and sediments to date in the literature. The composition profiles of PHCZs in the soils of MQ were dominated by 3,6-dichlorocarbazole (36-CCZ), 3-chlorocarbazole (3-CCZ), and 2-bromocarbazole (2-BCZ). The mean TOC-normalized concentrations of ΣPHCZs in soil samples from below-treeline (2092 ng/g TOC) were higher than those from alpine meadow (1124 ng/g TOC), probably due to the forest filter effect. The decreasing trend of the PHCZs TOC-normalized concentrations with altitude shows that accumulation of PHCZs from the alpine meadow samples was not affected by the mountain cold-trapping effect. Significantly positive correlations were observed between the concentrations of more than half of detected PHCZ congeners and TOC. In addition, PHCZs show the potential to represent a class of POPs with the frequent occurrence and wide distribution, as the abundance and environmental behavior of PHCZs are similar to some POPs in MQ.

Occurrence and potential sources of polyhalogenated carbazoles in farmland soils from the Three Northeast Provinces, China.

Polyhalogenated carbazoles (PHCZs) have been detected in various environments frequently and have attracted increasing attention for their multiple toxicities. However, only a few reports record the occurrence of PHCZs in farmland soils, and the sources of which were not yet been implemented. In this study, 12 PHCZs and carbazole (CZ) were screened in farmland soil samples from the Three Northeast Provinces, and the ∑PHCZs were in the range of 18.16-219.67 ng/g dw. 36-CCZ was the dominant congener (40.67%) in farmland soils, followed by 3-CCZ (14.51%), and average percentages of other congeners were lower than 10%. A concrete analysis of the sources of PHCZs in the soil was conducted, revealing the diversity of PHCZs sources. Potential toxic effects associated with the levels of PHCZs were evaluated via the toxic equivalency (TEQ) approach, and the TEQs of PHCZs (TEQPHCZs) were in the range of 2.24-14.06 pg TEQ/g dw. Notwithstanding the 1368-CCZ with a low concentration level, the mean contribution to TEQPHCZs was up to 24.24%, preceded only by 36-CCZ (39.69%), showing the congeners with low concentration also may pose potential risks to the environment. Partial PHCZs congeners (2-BCZ, 3-BCZ, 36-CCZ, 136-BCZ, and 2367-BCZ) showed significant correlations (r = 0.45-0.63, p < 0.05) with the total organic carbon (TOC). Significant correlations were shown between PHCZ congeners replaced by halogens of the same species and quantity (r = 0.40-0.99, p < 0.01). In view of the fact that the high concentration level of PHCZs in the soil and their source diversity, more environmental monitoring and risk assessments of PHCZs should be of particular concern.

Assembly strategies of the wheat root-associated microbiome in soils contaminated with phenanthrene and copper.

Plants can cope with stressful conditions by indirectly regulating root-associated microbial structures. However, the recruitment strategies of the root-associated microbiome in combined organic and inorganic contaminated soils are not well known, especially for common agricultural crops. In this study, we performed greenhouse experiments to investigate the interactive effects of joint copper (Cu) and phenanthrene (PHE) pollution on wheat growth and microbial detoxication processes. Results show that heavy metals did not affect PHE dissipation in the rhizosphere but significantly enhanced the accumulation of PHE in the endosphere. In contrast, the addition of PHE did not influence the absorption of Cu by wheat roots. Cu was the primary factor affecting the variation of microbial communities in cocontaminated treatments among each rhizocompartment while the interactive effects of combined pollutants were only detected in unplanted bulk soil. Microbes are known to degrade polycyclic aromatic hydrocarbons and tolerant heavy metal stress e.g. Novosphingobium, Sphingomonas, Sphingobium and Pseudomonas enriched in the contaminated treatments. Our results provide an integrated understanding of the synthetic effects of combined pollutants on the root-microbial assemblage process in plant-soil systems and offer useful information on the selection of effective bioremediating root-associated microbes for the application of self-remediation by common crops.

Complete genome sequence of Exiguobacterium mexicanum A-EM, isolated from seafloor hydrothermal vents in Atlantic Ocean.

Exiguobacterium mexicanum A-EM was isolated from seafloor hydrothermal vents(Caifan field, 14.0S 14.4 W) and was shown to degrade toxins and contaminants. Here, we present the complete genome sequence of A-EM, consisting of 2,412,492 bp, with a GC content of 53.16%. A-EM sequence contains genes encoding enzymes that degrade toxins and contaminants. Complete genome sequence of the strain A-EM can further provide insights into microbial adaption to the seafloor hydrothermal system and the genomic basis for the biotechnological application of strain A-EM as an efficient agent to degrade environmental contaminants.

Spatial distribution and influencing factors on the variation of bacterial communities in an urban river sediment.

The water and sediments of urban rivers are spatially heterogeneous because of the influence of environmental and anthropogenic factors. However, the spatial and functional diversity of bacterial communities in urban river sediments are unclear. We investigated the spatial distribution of microbial compositions in sediments in Qingdao section of the Dagu River, and the effects of sediment physiochemical properties on the variation were explored. Among the seven heavy metals analyzed, only the average concentration of Cd significantly exceeded the safety limit for sediments. The detailed composition and spatial distribution of bacterial communities fluctuated substantially between sites along the river. Bacterial datasets were separated into three clusters according to the environmental characteristics of sampling areas (the urbanized, scenic, and intertidal zones). For the urbanized zone, Acidobacteria, Firmicutes, Gemmatimonadetes, Bacteroidetes, and Gammaproteobacteria were significantly enriched, implying the effects of human activity. In the intertidal zone, Alphaproteobacteria and Deltaproteobacteria were significantly enriched, which are associated with S redox processes, as in the marine environment. Variation partitioning analysis showed that the amount of variation independently explained by variables of Na, Al, total S and Zn was largest, followed by sediment nutrients, while heavy metals and pH explained independently 13% and 9% of the variance, respectively. Overall, microbial structures in the Dagu River exhibited spatial variation and functional diversity as a result of natural and anthropogenic factors. The results will enable the prediction of the changes in urban river ecosystems that maintain their ecological balance and health.