In-Silico Selection of Wound-Healing Plant Secondary Molecules and Their Pro-Healing Activities on Experimental Models.

This study explored the potential of plant-derived molecules (PDMs) as a medicinal treatment for skin wounds. To assess their healing properties, 34 potential drug molecules (PDMs) and ten therapeutic targets were subjected to molecular docking and dynamics analysis, with allantoin used as a standard compound. Although aristolochic acid had the most potent inhibitory effect, its toxicity made it unsuitable for testing on cells and mice. Therefore, ß-caryophyllene (BC) and caryophyllene oxide (BCoxide) were chosen for further testing. The results showed that BC-treated HaCat cells had significantly improved scratch area closure, and both BC and BCoxide treatment produced positive effects such as reduced dermal cellularity and mast cells, decreased levels of inflammation markers IL-6 and TNF-α, and an increase in collagen deposition in mice tissues. However, these treatments did not accelerate wound healing. This study suggests that the PDMs selected based on in-silico results have significant potential for pro-healing abilities. It is essential to conduct further research to confirm our findings.

3D GIS for surface modelling of magnetic fields generated by overhead power lines and their validation in a complex urban area.

Residential exposure to magnetic fields generated by overhead high-voltage power lines, continues to be a matter of social concern and, for the scientific community, a challenge to model this exposure accurately enough to reliably detect even small effects in large populations complexes. In any expression of the magnetic field intensity, the source-receiver distance is a determining variable, especially in an environment closer to the electrical installation and critical with the existence of significant unevenness in the terrain. However, MF exposure studies adopt, due to their complexity, simplifications of reality where even sometimes the terrain relief and the buckling of the line are not considered. The application of 3D techniques with Geographic Information Systems (GIS) allows us to address this problem. This article presents a model for generating magnetic field intensity surfaces from high-precision terrain elevation data. The series expansion of the Biot-Savart law to an infinite rectilinear conductor with variable height according to the catenary described by the cables using ArcGIS software is applied to calculate the magnetic field. For the validation, 69 control points (1035 field measurements) were used in a free urban area and another 28 points (420 field measurements) in a built-up urban area with complex relief. Good estimates were obtained, although with differences in both areas. With MAPE 9.65% and 19.51%, R2 = 0.922 and 0.949, RMSE = 0.154 and 0.094 µT, respectively. Furthermore, 86% of the points were correctly classified according to usual exposure percentiles. However, the use of a 5 m resolution digital terrain model to obtain high-precision elevation data was an indispensable condition for the good performance of our model. The result as a continuous surface of magnetic field values at the real elevation of the ground can contribute significantly to the development of new environmental and public health studies.

Universal approach for full endoscopic decompression and percutaneous transpedicular fixation of the lumbar spine: A medial central approach (ACM).

ABSTRACT: Cohort study.This study aimed to determine the effectiveness of the universal approach of full endoscopy and percutaneous transpedicular fixation via a medial central approach (ACM) performed to surgically treat patients with lumbar degenerative surgical pathologies.Alternatives to interventionist treatments available to patients with lumbar degenerative surgical pathologies are related to recovery from minimally invasive surgery. Considering this, full endoscopic spinal decompression (full endoscopy) and percutaneous transpedicular fixation via an ACM represent advances in neurosurgical procedures, in particular, spinal surgery. Thus, the introduction of endoscopic and minimally invasive surgeries for the lumbar region has become 1 of the most important advances in modern surgery.A cohort of 79 patients undergoing full endoscopy and percutaneous transpedicular fixation was evaluated 6 times in 1 year. Pain intensity was measured using the visual analog scale (VAS), and lumbar functionality was measured using the Oswestry Disability Index (ODI). Six evaluations were performed: before surgery and on discharge after surgery as well as at 1, 3, 6, and 12 months after surgery.Before the ACM was applied, the VAS pain score was 8.52. At 11 hours post-surgery, the pain score reduced to 2.59 points (a difference of 5.73 points; P = 0.001). Of the 10 ODI domains evaluated, a difference was found between the period prior to surgery and 1 month later (P < 0.01).The universal approach to full endoscopy and lumbar percutaneous transpedicular fixation via an ACM is highly effective for patients with lumbar surgical degenerative pathologies.

Green Bank and Effelsberg Radio Telescope Searches for Axion Dark Matter Conversion in Neutron Star Magnetospheres.

Axion dark matter (DM) may convert to radio-frequency electromagnetic radiation in the strong magnetic fields around neutron stars. The radio signature of such a process would be an ultranarrow spectral peak at a frequency determined by the mass of the axion particle. We analyze data we collected from the Robert C. Byrd Green Bank Telescope in the L band and the Effelsberg 100-m Telescope in the L band and S band from a number of sources expected to produce bright signals of axion-photon conversion, including the Galactic center of the Milky Way and the nearby isolated neutron stars RX J0720.4-3125 and RX J0806.4-4123. We find no evidence for axion DM and are able to set constraints on the existence of axion DM in the highly motivated mass range between ∼5 and 11 µeV with the strongest constraints to date on axions in the ∼10-11 µeV range.

Conus Medullaris Level in Vertebral Columns With Lumbosacral Transitional Vertebra.

BACKGROUND: The estimated prevalence of lumbar or sacral transitional vertebrae (LSTV) in the population is 4% to 30%. Few small patient series have studied the normal level of the conus medullaris (CM) in individuals with LSTV. OBJECTIVE: To determine, by using a large cohort of patients, whether individuals of all ages with LSTV have different CM positions in the spinal canal in comparison with the rest of the population with normal vertebral columns. METHODS: We performed an institutional retrospective analysis of spinal magnetic resonance images on individuals with LSTV of all ages, sexes, and pathologies during a 10-year period. Fifty-seven percent of patients (n = 467) had a lumbarized vertebra and 43% had sacralized vertebra (n = 355). Mean age at the time of the study was 55 ± 19 years (range 1-97 years). Fifty-two percent were male and 48% were female. Sixty percent of subjects with a sacralized vertebra were female, and 54.5% of those with a lumbarized vertebra were male (P = .001). RESULTS: The CM in individuals with a lumbarized vertebra was seen to be lower at L1-2 to L2s, than un those with a sacralized vertebra where most conuses were at T12-L1 to L1s (P ≤ 0.001). The CM level was similarly distributed among sexes and ages. CONCLUSION: In our series, the CM level, when lumbarization occurred, was lower, with a mean level at L1-L2, whereas a more superior mean level at T12-L1 was seen when sacralization occurred. CM level was not influenced by sex, age, or pathology other than tethered cords.

HIV treatment cascade among transgender women in a San Francisco respondent driven sampling study.

OBJECTIVE: Male-to-female transgender women (transwomen) have a disproportionate burden of HIV. We sought to estimate HIV treatment cascade indicators among transwomen in San Francisco. METHODS: We conducted a respondent driven sampling (RDS) study of 314 transwomen from August to December 2010. The study tested participants for HIV and collected self-reported data on linkage and access to care, viral load and antiretroviral treatment (ART). We derived population-based estimates and 95% CIs of cascade indicators using sampling weights based on established RDS methods. We conducted RDS-weighted logistic regression analyses to evaluate correlates of being on ART and being virologically suppressed (viral load ≤ 200 copies/mL). RESULTS: The RDS-weighted population-based estimate of HIV prevalence was 39% (95% CI 32% to 48%) among transwomen tested for HIV. Among HIV-positive transwomen, 77% (95% CI 70% to 93%) reported being linked to care within 3 months of diagnosis and 87% (95% CI 76% to 98%) accessed care in the past 6 months. In addition, 65% (95% CI 54% to 75%) were on ART, and less than half (44%; 95% CI 21% to 58%) were virologically suppressed. Housing instability was associated with lower odds of being on ART and being virologically suppressed. CONCLUSIONS: We observed a high prevalence of HIV in our population-based estimates of transwomen in San Francisco, coupled with modest ART use and low virological suppression rates, indicating high potential for forward transmission. Poor HIV treatment outcomes were consistently associated with housing instability. These data suggest that multi-level efforts, including efforts to address housing insecurity, are urgently needed to ameliorate disparities in HIV clinical outcomes among transwomen and reduce secondary HIV transmission to their partners.

Alcohol and substance use among transgender women in San Francisco: prevalence and association with human immunodeficiency virus infection.

INTRODUCTION AND AIMS: Alcohol and substance use can have negative health consequences among both human immunodeficiency virus (HIV)-positive and -negative individuals, and are associated with behaviors that facilitate HIV transmission and acquisition. The relationship of substance use and HIV is well documented among key populations at risk for HIV. However, although transwomen (male-to-female transgender) are disproportionately impacted by HIV, this overlap remains understudied in this population. We sought to evaluate the association between HIV, alcohol and substance use among transwomen. DESIGN AND METHODS: We conducted a secondary data analysis of Respondent Driven Sampling study which collected information on self-reported alcohol and substance use among 314 transwomen. We used multivariable logistic regression to assess relationship between HIV infection and classes and patterns of alcohol and substance use. RESULTS: We found that 58% of transwomen used alcohol, and 43.3% used substances. The most common substances used were: marijuana (29%), methamphetamine (20.1%), crack cocaine (13.4%), and 'club drugs' (13.1%). Transwomen who reported any methamphetamine use [adjusted odds ratio (AOR) 3.02 (95% confidence interval (CI) = 1.51-6.02)], methamphetamine use before or during anal intercourse [AOR 3.27 (95% CI = 1.58-6.77)], and at least weekly methamphetamine use [AOR 3.89 (95% CI = 1.64-9.23)] had significantly greater odds of testing positive for HIV. DISCUSSION AND CONCLUSIONS: Transfemales have high prevalence of alcohol and substance use; those tested positive for HIV used significantly more methamphetamine in general, and in conjunction with sex. Given the disproportionate prevalence of HIV and substance use in this population, interventions aimed at addressing both substance use and HIV risk among transwomen are urgently needed.