Characterization of phytoplankton functional groups in a tropical shellfish harvesting estuary (Ashtamudi) and adjacent nearshore waters (southwest coast of India).

A pioneering study on phytoplankton marker pigments, by adopting the HPLC-CHEMTAX analytical approach, was carried out in one of the major shellfish harvesting estuaries (Ashtamudi estuary-AE) on the southwest coast of India and also its adjacent nearshore waters (< 20 m depth) to study the dynamics of phytoplankton functional groups (PFGs). The AE, in general, appeared to be warm (> 29 °C) during the non-monsoon seasons, along with the prevalence of higher salinity (> 25) and NH4-N (> 5 µM) levels. However, during the summer monsoon (SM), the prevailing substantial river influx converted the AE into a low salinity (< 10) dominated system, provided with enhanced levels of NO3-N (4.6-12.6 µM) and PO4-P (0.2-1.4 µM), specifically on its upper reaches. The dissolved inorganic nitrogen (DIN) in the AE was mainly comprised of ammonium (NH4-N), approximately up to ~ 81.8% of the DIN, regardless of seasons, which signifies the eutrophic state of the estuary, the upstream sampling locations, in particular. Concerning the phytoplankton community, a pronounced spatio-temporal variation in chlorophyll a biomass was discernible in the AE, with an exceptional increase (6.4-12.1 mg m-3) during the SIM period. The prevalence of a conspicuous increase in chlorophyll a (av. > 5 mg m-3) along with enhanced zeaxanthin (av. > 1.5 mg m-3) recorded in the AE during the non-monsoon (specifically SIM period) season apparently signified the characteristic governance of cyanobacterial community. During the SM period, the estuary sustained more or less similar concentrations of certain marker pigments, i.e. alloxanthin, zeaxanthin, fucoxanthin and chl b, which representing the co-occurrence of cryptophytes, cyanobacteria, diatoms and chlorophytes, respectively. In contrast, the nearshore waters, wherein enhanced nitrate (NO3-N) and phosphate (PO4-P) levels prevailed, irrespective of seasons, sustained dominance of fucoxanthin over other marker pigments, which indicated the numerical supremacy of diatoms. The CHEMTAX analysis, adopted for estimating the chlorophyll a equivalents of various PFGs, corroborated the supremacy of cyanobacterial derived chlorophyll a in the estuary, and it was conspicuous during the non-monsoonal seasonal periods. Even though the estuary has shown remarkable spatio-temporal hydrographic inconsistencies, that variability was not much operative in generating extreme changes in the nutrient components and subsequent phytoplankton community compositions. From the conspicuous increase in N:P and Si:P ratios, resulting mainly from the low levels of P, it can be concluded that the AE has been a P-limited system for phytoplankton growth (especially for large-sized phytoplankton, e.g. diatoms and dinoflagellates), mainly during the non-monsoonal seasonal periods. Perhaps, this P-limitation, along with the prevalence of warm water column and enhanced NH4-N levels, could be the potential causes of the preponderance of cyanobacterial populations in the AE.

Ecological habitat quality assessment of a tropical estuary using macrobenthic functional characteristics and biotic indices.

Seasonal variation in the macrobenthic functional characteristics, such as trophic structure and secondary production, was studied, along with their structural characteristics such as density, biomass, and community distribution in Cochin estuary (CE), a tropical monsoonal estuary along the Southwest coast of India during 2018-2019. The biotic indices, AMBI (AZTI's Marine Biotic Index) and M-AMBI (Multivariate-AMBI) were used to assess the ecological quality of the study area by using the sensitivity of macrobenthic fauna to disturbances. A distinct temporal variation was evident in the macrobenthic structural characteristics, wherein high density, biomass, and species richness were observed during the post-monsoon. Polycheate species were dominant during pre-monsoon (Prionospio cirrifera) and monsoon (Mediomastus sp.), while gastropod species, Stenothyra perpumila, was dominant during post-monsoon. The trophic structure analysis revealed the dominance of deposit-feeding polychaetes during the pre-monsoon in association with the fine sediments laden with high organic carbon. In contrast, during post-monsoon, the polychaete trophic groups were more or less evenly distributed in the sandy substratum. The higher density of suspension feeders and herbivorous-grazers observed during the post-monsoon indicated improved habitat quality compared to the pre-monsoon dominated by the detritivores. The macrobenthic secondary production was also high during the post-monsoon, contributed by a diverse assemblage of molluscs, crustaceans, and polychaetes having size > 0.7 mm. The biotic indices also showed an evident increase in the habitat quality of the estuary from pre-monsoon to post-monsoon (AMBI, moderately disturbed to undisturbed; M-AMBI, poor-moderate-good to good-high conditions), similar to the results of benthic structural and functional characteristics. The study highlights the significance of the utility of benthic functional characteristics while assessing the habitat quality of an ecosystem.