Microbial risk assessment of ready-to-eat mixed vegetable salads from different restaurants of Bangladesh Agricultural University campus.

OBJECTIVE: The study was aimed to analyze the microbiological quality of mixed vegetable salads and to understand the risk related with its consumption from different restaurants around Bangladesh Agricultural University campus in Mymensingh. MATERIALS AND METHODS: Sixty (60) samples of mixed vegetable salads were taken from twelve (12) different restaurants in five different time points from each restaurant. In parallel, restaurant workers were asked for handling practices while the consumers were interviewed about their salad consumption pattern and whether they had experienced any health-related problems. Microbial risk assessment of Staphylococcus spp., Salmonella spp., and Escherichia coli was estimated by Monte Carlo simulation (10,000 iterations), an exponential model. RESULTS: Aerobic plate count was ranged from 7.73 ± 0.61 to 9.04 ± 0.26 log cfu/gm, Staphylococcus spp. from 4.64 ± 0.61 to 6.42 ± 0.53 log cfu/gm, Salmonella spp. from 4.75 ± 0.08 to 5.27 ± 0.53 log cfu/gm, and E. coli from 4.98 ± 0.20 to 6.66 ± 0.80 log cfu/gm. From the survey, it was found that total consumers had 18% chances where the male had 13% and the female had 30% chances of being infected with salads. Again frequent, average, and occasional consumers had 31%, 13%, and 0% chances, respectively, of being infected with those salads. From the Monte Carlo simulation, the calculated mean annual risks of Staphylococcus spp., Salmonella spp., and E. coli infection for the three exposure scenarios were found to be about 100%. CONCLUSION: The study actually revealed the potential microbial contamination in mixed vegetable salads which may impact on food safety and human health. So, the findings suggest that following hygienic measures during processing and handling the microbiological quality of mixed vegetables salads can be improved.

WOMEN'S EMPOWERMENT AND INTIMATE PARTNER VIOLENCE IN BANGLADESH: INVESTIGATING THE COMPLEX RELATIONSHIP.

The aim of this study was to assess whether women's empowerment ensures reduced intimate partner violence (IPV). The differential relationship between women's empowerment and IPV among married women in Bangladesh was investigated using 2007 Demographic and Health Survey data. Logistic regression models were used to assess whether women's empowerment had any influence on their likelihood of experiencing IPV while controlling for other covariates. The analyses revealed that older cohorts of women, who were more empowered, were more vulnerable to physical violence than less-empowered, younger women. The likelihood of being affected by physical violence among less-empowered, childless women was greater than that of more-empowered women with only male children. Less-empowered, uneducated women were more likely to experience physical violence than more-empowered, primary-educated women. Less-empowered women who had been married for less than 5 years were more likely to suffer from physical violence than more-empowered women who had been married for more than 19 years. The likelihood of experiencing sexual violence was not found to have any significant association with women's empowerment. The findings suggest that although women's empowerment in Bangladesh is gradually improving, some sub-groups of empowered women are still susceptible to IPV.

Arsenic exposure to dairy cows in Bangladesh.

Food-chain contamination by arsenic (As) is a newly uncovered disaster. Effects of As-contaminated drinking water and paddy straw on the excretion of As through milk, urine, and dung of dairy cows (n = 240) were studied in As-prone areas of Bangladesh. Mean (±SEM) total As (inorganic plus organic) concentration in drinking water, paddy straw [dry weight dw)], cow's urine (specific gravity adjusted to 1.035), dung (dw), and milk (wet weight) were 89.6 ± 6.5 µg/l, 1,114.4 ± 57.3 µg/kg, 123.6 ± 7.6 µg/l, 1,693.0 ± 65.1 µg/kg, and 26.2 ± 2.8 µg/l, respectively. Significantly (p < 0.01) greater As was in Boro straw (1,386.9 ± 71.8 µg/kg) than Aus (702.4 ± 67.1 µg/kg) and Aman (431.7 ± 28.8 µg/kg) straw and in straw irrigated with shallow (1,697.3 ± 81.9 µg/kg) than deep well water (583.6 ± 62.7 µg/kg) and surface water (511.8 ± 30.0 µg/kg). Significant (p < 0.01) positive correlations were found between As contents of cow's urine and drinking water (r = 0.92) as well as cow dung and straw (r = 0.82). Concentrations of As in cow urine, dung, and milk were increased with the relative increment of As in drinking water and/or straw. These results provide evidence that dairy cows excrete ingested As mainly through urine and dung; thus, As biotransformation through milk remains low. This low concentration of As in milk may be of concern when humans are exposed to multiple sources of As simultaneously. Moreover, As in cow dung could be an environmental issue in Bangladesh.

Arsenic in eggs and excreta of laying hens in Bangladesh: a preliminary study.

The aim of this study was to detect arsenic concentrations in feed, well-water for drinking, eggs, and excreta of laying hens in arsenic-prone areas of Bangladesh and to assess the effect of arsenic-containing feed and well-water on the accumulation of arsenic in eggs and excreta of the same subject. One egg from each laying hen (n = 248) and its excreta, feed, and well-water for drinking were collected. Total arsenic concentrations were determined by atomic absorption spectrophotometer, coupled with hydride generator. Effects of arsenic-containing feed and drinking-water on the accumulation of arsenic in eggs and excreta were analyzed by multivariate regression model, using Stata software. Mean arsenic concentrations in drinking-water, feed (dry weight [DW]), egg (wet weight [WW]), and excreta (DW) of hens were 77.3, 176.6, 19.2, and 1,439.9 ppb respectively. Significant (p < 0.01) positive correlations were found between the arsenic contents in eggs and drinking-water (r = 0.602), drinking-water and excreta (r = 0.716), feed and excreta (r = 0.402) as well as between the arsenic content in eggs and the age of the layer (r = 0.243). On an average, 55% and 82% of the total variation in arsenic contents of eggs and excreta respectively could be attributed to the variation in the geographic area, age, feed type, and arsenic contents of drinking-water and feed. For each week's increase in age of hens, arsenic content in eggs increased by 0.94%. For every 1% elevation of arsenic in drinking-water, arsenic in eggs and excreta increased by 0.41% and 0.44% respectively whereas for a 1% rise of arsenic in feed, arsenic in eggs and excreta increased by 0.40% and 0.52% respectively. These results provide evidence that, although high arsenic level prevails in well-water for drinking in Bangladesh, the arsenic shows low biological transmission capability from body to eggs and, thus, the value was below the maximum tolerable limit for humans. However, arsenic in drinking-water and/or feed makes a significant contribution to the arsenic accumulations in eggs and excreta of laying hens.