Arctic plasmidome analysis reveals distinct relationships among associated antimicrobial resistance genes and virulence genes along anthropogenic gradients.

Polar regions are relatively isolated from human activity and thus could offer insight into anthropogenic and ecological drivers of the spread of antibiotic resistance. Plasmids are of particular interest in this context given the central role that they are thought to play in the dissemination of antibiotic resistance genes (ARGs). However, plasmidomes are challenging to profile in environmental samples. The objective of this study was to compare various aspects of the plasmidome associated with glacial ice and adjacent aquatic environments across the high Arctic archipelago of Svalbard, representing a gradient of anthropogenic inputs and specific treated and untreated wastewater outflows to the sea. We accessed plasmidomes by applying enrichment cultures, plasmid isolation and shotgun Illumina sequencing of environmental samples. We examined the abundance and diversity of ARGs and other stress-response genes that might be co/cross-selected or co-transported in these environments, including biocide resistance genes (BRGs), metal resistance genes (MRGs), virulence genes (VGs) and integrons. We found striking differences between glacial ice and aquatic environments in terms of the ARGs carried by plasmids. We found a strong correlation between MRGs and ARGs in plasmids in the wastewaters and fjords. Alternatively, in glacial ice, VGs and BRGs genes were dominant, suggesting that glacial ice may be a repository of pathogenic strains. Moreover, ARGs were not found within the cassettes of integrons carried by the plasmids, which is suggestive of unique adaptive features of the microbial communities to their extreme environment. This study provides insight into the role of plasmids in facilitating bacterial adaptation to Arctic ecosystems as well as in shaping corresponding resistomes. Increasing human activity, warming of Arctic regions and associated increases in the meltwater run-off from glaciers could contribute to the release and spread of plasmid-related genes from Svalbard to the broader pool of ARGs in the Arctic Ocean.

The regime of constructed wetlands in greywater treatment.

There is an excellent need for supply-side threats due to the enhanced degradation and reclamation of existing water bodies in the present scenario. This led to the global water crisis. One of the easiest ways to fulfil the growing need for freshwater is the recycling of wastewater. Greywater is a form of wastewater from households, industries, etc., with some less toxic materials. The recycling of this greywater has provoked the development of new and sustainable technologies to meet the growing water demand. Engineered constructed wetlands are considered one of the most economically practical processes to treat greywater due to its minimal footprint. In this case study, we summarize several categories of constructed wetlands, operating conditions, and the effects of biological, physical, and chemical aspects of greywater on their treatment performance. On the other hand, the effluent quality from diverse wetlands is also summarized. Furthermore, it would be better to consider that constructed wetlands' integrated performance with disinfection may improve the effluent quality to desirable standards.