1.
STAR Protoc
; 2(3): 100722, 2021 09 17.
Artigo
em Inglês
| MEDLINE
| ID: mdl-34458865
RESUMO
Intravital multiphoton imaging of the tumor milieu allows for the dissection of intricate and dynamic biological processes in situ. Herein, we present a step-by-step protocol for setting up an experimental cancer imaging model that has been optimized for solid tumors such as breast cancer and melanoma implanted in the flanks of mice. This protocol can be utilized for dissecting tumor-immune cell dynamics in vivo or other tumor-specific biological questions. For complete details on the use of this protocol for intravital imaging of breast cancer, please refer to Tikoo et al. (2021a), and for intravital imaging of melanoma, please refer to Tikoo et al. (2021b).
Assuntos
Microscopia Intravital/métodos , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Microambiente Tumoral/fisiologia , Animais , Neoplasias da Mama/diagnóstico por imagem , Feminino , Melanoma/diagnóstico por imagem , Camundongos
2.
J Invest Dermatol
; 141(8): 2090-2094.e6, 2021 08.
Artigo
em Inglês
| MEDLINE
| ID: mdl-33675788