RESUMO
We present a microscope on chip for automated imaging of Drosophila embryos by light sheet fluorescence microscopy. This integrated device, constituted by both optical and microfluidic components, allows the automatic acquisition of a 3D stack of images for specimens diluted in a liquid suspension. The device has been fully optimized to address the challenges related to the specimens under investigation. Indeed, the thickness and the high ellipticity of Drosophila embryos can degrade the image quality. In this regard, optical and fluidic optimization has been carried out to implement dual-sided illumination and automatic sample orientation. In addition, we highlight the dual color investigation capabilities of this device, by processing two sample populations encoding different fluorescent proteins. This work was made possible by the versatility of the used fabrication technique, femtosecond laser micromachining, which allows straightforward fabrication of both optical and fluidic components in glass substrates.
Assuntos
Drosophila , Microfluídica , Animais , Lasers , Microscopia de Fluorescência , MicrotecnologiaRESUMO
In this work we demonstrate novel integrated-optics modulators and switches, realized in a glass substrate by femtosecond laser pulses. These devices are based on oscillating microcantilevers, machined by water-assisted laser ablation. Single-mode optical waveguides are laser-inscribed inside the cantilever beam and continue in the substrate beyond the cantilever's tip. By exciting the resonant oscillation of the mechanical structure, coupling between the waveguide segments is varied in time. Operation frequencies are in the range of tens of kilohertz, thus they markedly overcome the response-time limitation of other glass-based modulators, which rely on the thermo-optic effect. These components may be integrated in more complex waveguide circuits or optofluidic lab-on-chips, to provide periodic and high-frequency modulation of the optical signals.
