Sequential analysis of δ15 N in guard hair suggests late gestation is the most critical period for muskox calf recruitment.

RATIONALE: Analysis of stable isotopes in tissue and excreta may provide information about animal diets and their nutritional state. As body condition may have a major influence on reproduction, linking stable isotope values to animal demographic rates may help unravel the drivers behind animal population dynamics. METHODS: We performed sequential analysis of δ15 N values in guard hair from 21 muskoxen (Ovibos moschatus) from Zackenberg in high arctic Greenland. We were able to reconstruct the dietary history for the population over a 5-year period with contrasting environmental conditions. We examined the linkage between guard hair δ15 N values in 12 three-month periods and muskox calf recruitment to detect critical periods for muskox reproduction. Finally, we conducted similar analyses of the correlation between environmental conditions (snow depth and air temperature) and calf recruitment. RESULTS: δ15 N values exhibited a clear seasonal pattern with high levels in summer and low levels in winter. However, large inter-annual variation was found in winter values, suggesting varying levels of catabolism depending on snow conditions. In particular δ15 N values during January-March were linked to muskox recruitment rates, with higher values coinciding with lower calf recruitment. δ15 N values were a better predictor of muskox recruitment rates than environmental conditions. CONCLUSIONS: Although environmental conditions may ultimately determine the dietary δ15 N signal in muskox guard hairs, muskox calf recruitment was more strongly correlated with δ15 N values than ambient snow and temperature. The period January-March, corresponding to late gestation, appears particularly critical for muskox reproduction.

Muskoxen homogenise soil microbial communities and affect the abundance of methanogens and methanotrophs.

Grazing herbivores may affect soil microbial communities indirectly by impacting soil structure and vegetation composition. In high arctic environments, this impact is poorly elucidated, while having potentially wide-reaching effects on the ecosystem. This study examines how a key arctic herbivore, the muskox Ovibos moschatus, affects the soil microbial community in a high arctic fen. Environmental DNA was extracted from soil samples taken from grazed control plots and from muskox exclosures established 5 years prior. We sequenced amplicons of the 16S rRNA gene to provide insight into the microbial communities. We found that in the grazed control plots, microbial communities exhibited high evenness and displayed highly similar overall diversity. In plots where muskoxen had been excluded, microbial diversity was significantly reduced, and had more uneven intra-sample populations and overall lower ecological richness and evenness. We observed that the composition of microbial communities in grazed soils were significantly affected by the presence of muskoxen, as seen by elevated relative abundances of Bacteroides and Firmicutes, two major phyla found in muskox faeces. Furthermore, an increase in relative abundance of bacteria involved in degradation of recalcitrant carbohydrates and cycling of nitrogen was observed in grazed soil. Ungrazed soils displayed increased abundances of bacteria potentially involved in anaerobic oxidation of methane, whereas some methanogens were more abundant in grazed soils. This corroborates a previous finding that methane emissions are higher in arctic fens under muskox grazing. Our results show that the presence of large herbivores stimulates soil microbial diversity and has a homogenizing influence on the inter-species dynamics in soil microbial communities. The findings of this study, thus, improve our understanding of the effect of herbivore grazing on arctic ecosystems and the derived methane cycling.

Muskox status, recent variation, and uncertain future.

Muskoxen (Ovibos moschatus) are an integral component of Arctic biodiversity. Given low genetic diversity, their ability to respond to future and rapid Arctic change is unknown, although paleontological history demonstrates adaptability within limits. We discuss status and limitations of current monitoring, and summarize circumpolar status and recent variations, delineating all 55 endemic or translocated populations. Acknowledging uncertainties, global abundance is ca 170 000 muskoxen. Not all populations are thriving. Six populations are in decline, and as recently as the turn of the century, one of these was the largest population in the world, equaling ca 41% of today's total abundance. Climate, diseases, and anthropogenic changes are likely the principal drivers of muskox population change and result in multiple stressors that vary temporally and spatially. Impacts to muskoxen are precipitated by habitat loss/degradation, altered vegetation and species associations, pollution, and harvest. Which elements are relevant for a specific population will vary, as will their cumulative interactions. Our summaries highlight the importance of harmonizing existing data, intensifying long-term monitoring efforts including demographics and health assessments, standardizing and implementing monitoring protocols, and increasing stakeholder engagement/contributions.

EVALUATION OF TWO ENZYME-LINKED IMMUNOSORBENT ASSAYS MEASURING PREGNANCY-ASSOCIATED GLYCOPROTEINS IN THE BLOOD OF MUSKOXEN ( OVIBOS MOSCHATUS).

Pregnancy-associated glycoproteins (PAGs) are expressed by the ruminal placenta, making their detection in blood an accurate indicator of pregnancy. This study aimed to evaluate two commercially available PAG enzyme-linked immunosorbent assays (ELISAs) in muskoxen ( Ovibos moschatus). The two tests are based on the same principles; however, one is evaluated photometrically and the other visually. Sixteen samples covering all trimesters of pregnancy, and 16 nonpregnant samples were included to evaluate test performance. Both tests reliably detected pregnancy. The photometric ELISA showed a sensitivity and specificity of 94% and 100%, respectively. Although the visual ELISA depends on somewhat subjective interpretations, it came up with a sensitivity of 81% and a specificity of 100%, and might thus provide a useful in-house tool when limited laboratory equipment is available. Analysis of additional samples showed consistent results during pregnancy and circulating PAGs for at least 18 days postpartum.

Show Me Your Rump Hair and I Will Tell You What You Ate - The Dietary History of Muskoxen (Ovibos moschatus) Revealed by Sequential Stable Isotope Analysis of Guard Hairs.

The nutritional state of animals is tightly linked to the ambient environment, and for northern ungulates the state strongly influences vital population demographics, such as pregnancy rates. Continuously growing tissues, such as hair, can be viewed as dietary records of animals over longer temporal scales. Using sequential data on nitrogen stable isotopes (δ15N) in muskox guard hairs from ten individuals in high arctic Northeast Greenland, we were able to reconstruct the dietary history of muskoxen over approximately 2.5 years with a high temporal resolution of app. 9 days. The dietary chronology included almost three full summer and winter periods. The diet showed strong intra- and inter-annual seasonality, and was significantly linked to changes in local environmental conditions (temperature and snow depth). The summer diets were highly similar across years, reflecting a graminoid-dominated diet. In contrast, winter diets were markedly different between years, a pattern apparently linked to snow conditions. Snow-rich winters had markedly higher δ15N values than snow-poor winters, indicating that muskoxen had limited access to forage, and relied more heavily on their body stores. Due to the close link between body stores and calf production in northern ungulates, the dietary winter signals could eventually serve as an indicator of calf production the following spring. Our study opens the field for further studies and longer chronologies to test such links. The method of sequential stable isotope analysis of guard hairs thus constitutes a promising candidate for population-level monitoring of animals in remote, arctic areas.