RESUMO
The morbidity due to congenital toxoplasmosis in humans is very high. Most of these infected children are likely to develop symptoms of clinical toxoplasmosis. Sequelae in fetus resulting from Toxoplasma gondii infections in women who become infected with this parasite during pregnancy can be devastating and enormous efforts are directed in some countries to prevent these consequences. Here, an update on congenital toxoplasmosis in humans, especially the rate of congenital infections in humans worldwide, is provided. Although several countries have surveillance programmes, most information on the rate of congenital transmission is from France and Brazil. Because of compulsory national screening programme in France to detect and treat women with recently acquired T. gondii infection with anti-toxoplasma therapy, the rate of congenital transmission and the severity of disease in children are declining. Infections by this parasite are widely prevalent in Brazil. The severity of clinical toxoplasmosis in Brazilian children is very high and may be associated with the genetic characteristics of T. gondii isolates prevailing in animals and humans in Brazil. Virtually little or no information is available on this topic from China, India and other countries in Asia.
Assuntos
Doenças Transmissíveis , Toxoplasma , Toxoplasmose Congênita , Toxoplasmose , Animais , Feminino , Humanos , Índia , Gravidez , Toxoplasma/genética , Toxoplasmose/epidemiologia , Toxoplasmose Congênita/epidemiologia , Toxoplasmose Congênita/prevenção & controleRESUMO
Toxoplasma gondii infections are common in humans and animals worldwide. The present review summarizes worldwide information on the prevalence of clinical and subclinical infections, epidemiology, and genetic diversity of T. gondii infections in bears. Seroprevalence estimates of T. gondii in black bears (Ursus americanus) are one of the highest of all animals. In Pennsylvania, seroprevalence is around 80% and has remained stable for the past 4 decades. Approximately 3,500 bears are hunted yearly in Pennsylvania alone. The validity of different serological tests is discussed based on bioassay and serological comparisons. Seroprevalence in grizzly bears (Ursus arctos) is lower than that in black bears. Even polar bears (Ursus maritimus) are infected; infections in these animals are ecologically interesting because of the absence of felids in the Arctic. Clinical toxoplasmosis in bears is rare and not documented in adult animals. The few reports of fatal toxoplasmosis in young bears need confirmation. Viable T. gondii has been isolated from black bears and a grizzly bear. The genetic diversity of isolates based on DNA from viable T. gondii isolates is discussed. Genetic typing of a total of 26 T. gondii samples from bears using 10 PCR-RFLP markers revealed 8 PCR-RFLP ToxoDB genotypes: #1 (clonal type II) in 3 samples, #2 (clonal type III) in 8 samples, #4 (haplogroup 12) in 3 samples, #5 (haplogroup 12) in 3 samples, #74 in 5 samples, #90 in 1 sample, #147 in 1 sample, and #216 in 2 samples. These results suggest relatively high genetic diversity of T. gondii in bears. Overall, T. gondii isolates in bears range from those circulating in a domestic cycle (genotypes #1 and #2) to those mainly associated with wildlife (such as genotypes #4 and #5, together known as haplogroup 12). A patient who acquired clinical Trichinella spiralis infection after eating undercooked bear meat also acquired T. gondii infection. Freezing of infected meat kills T. gondii, including the strains isolated from bears.
Assuntos
Toxoplasmose Animal/epidemiologia , Ursidae/parasitologia , Fatores Etários , Animais , Anticorpos Antiprotozoários/sangue , Variação Genética , Humanos , Prevalência , Fatores de Risco , Estudos Soroepidemiológicos , Fatores Sexuais , Toxoplasma/genética , Toxoplasma/imunologia , Toxoplasmose Animal/transmissão , Estados Unidos/epidemiologia , Ursidae/genéticaRESUMO
Toxoplasma gondii infections are common in humans and animals worldwide. The ingestion of food or water contaminated with oocysts excreted by infected cats or ingestion of uncooked or undercooked meat containing tissue cysts of T. gondii are the 2 major modes of transmission of T. gondii. Deer are a popular game. Recently, outbreaks of clinical toxoplasmosis were reported in humans in North America linked to ingestion of undercooked venison. Here, we review prevalence, persistence of infection, clinical disease, epidemiology, and public health risks of T. gondii infections in deer and other cervids for the past decade. Estimates of worldwide serological prevalence are summarized individually for each species of deer, elk, moose, and caribou. Genetic diversity of 112 viable isolates of T. gondii from cervids is discussed, including its public health significance. Prevalence of T. gondii in deer is very high. Any part of a deer, including liver, spleen, and muscles, should be cooked thoroughly before human consumption.
Assuntos
Cervos/parasitologia , Carne/parasitologia , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/epidemiologia , Toxoplasmose Animal/transmissão , Toxoplasmose/etiologia , Aborto Animal/epidemiologia , Animais , Anticorpos Antiprotozoários/sangue , Culinária/métodos , Culinária/normas , DNA de Protozoário/análise , Genótipo , Humanos , Fígado/parasitologia , Músculos/parasitologia , Prevalência , Baço/parasitologia , Toxoplasma/classificação , Toxoplasma/genética , Toxoplasma/imunologia , Toxoplasmose/epidemiologia , Toxoplasmose/transmissão , Estados Unidos/epidemiologiaRESUMO
Toxoplasma gondii infections are common in humans and animals worldwide. Rodents are one of the most important intermediate hosts for T. gondii because they are preyed on by cats, who in turn excrete the environmentally resistant oocysts in their feces and thus spread the infection. Information on T. gondii infections is spread in numerous reports and is not easily accessible to readers. Here, we review prevalence, persistence of infection, clinical disease, epidemiology, and genetic diversity of T. gondii infections in wild rodents worldwide. Data are tabulated by country, by each rodent species alphabetically, and chronologically. Recent genetic diversity of T. gondii strains in rodents is critically evaluated.
Assuntos
Doenças dos Roedores/epidemiologia , Doenças dos Roedores/parasitologia , Toxoplasmose Animal/epidemiologia , Animais , Animais Selvagens/parasitologia , Bioensaio/veterinária , Brasil/epidemiologia , DNA de Protozoário/isolamento & purificação , Variação Genética , Camundongos , Prevalência , Roedores , Estudos Soroepidemiológicos , Toxoplasma/classificação , Toxoplasma/genéticaRESUMO
Toxoplasma gondii infections are common in humans and animals worldwide. Wild and domestic avian species are important in the epidemiology of T. gondii infections because felids prey on them and excrete millions of oocysts in the environment, disseminating the infection. Herbivorous birds are also excellent sentinels of environmental contamination with T. gondii oocysts because they feed on the ground. Toxoplasma gondii infections in birds of prey reflect infections in intermediate hosts. Humans can become infected by consuming undercooked avian tissues. Here, the authors reviewed prevalence, persistence of infection, clinical disease, epidemiology and genetic diversity of T. gondii strains isolated from turkeys, geese, ducks, ratites and avian species (excluding chickens) worldwide 2009-2020. Genetic diversity of 102 T. gondii DNA samples isolated worldwide is discussed. The role of migratory birds in dissemination of T. gondii infection is discussed.
Assuntos
Aves/parasitologia , Toxoplasma , Toxoplasmose Animal/epidemiologia , Migração Animal , Animais , Animais Selvagens/parasitologia , Doenças das Aves/epidemiologia , Galinhas/parasitologia , DNA de Protozoário , Reservatórios de Doenças/parasitologia , Patos/parasitologia , Variação Genética , Humanos , Oocistos , Paleógnatas/parasitologia , Prevalência , Toxoplasma/genética , Toxoplasma/isolamento & purificação , Perus/parasitologiaRESUMO
Toxoplasma gondii infections are common in humans and animals worldwide. The ingestion of food or water contaminated with oocysts excreted by infected cats or ingesting uncooked or undercooked meat containing tissue cysts of T. gondii are the 2 major modes of transmission of T. gondii. Viable T. gondii is more prevalent in pork and lamb than in beef. In the past decade, there have been many articles on the high seroprevalence in cattle, particularly from China. There is a report of an outbreak of acute toxoplasmosis in humans suspected to be linked to the ingestion of Artisan fresh cheese from cow's milk. There are conflicting reports concerning the rate of congenital transmission of T. gondii in cattle, especially from Brazil. In a report from Brazil, viable T. gondii was isolated from the blood of 1 of 60 pregnant cows slaughtered at an abattoir and from 1 fetus. The role of beef in the epidemiology of T. gondii infections is still not clear. Here, we review prevalence, persistence of infection, clinical disease, epidemiology, and public health risks of T. gondii infections in cattle from beef and cow's milk worldwide for the past decade.
Assuntos
Doenças dos Bovinos/parasitologia , Saúde Pública , Toxoplasmose Animal/parasitologia , Animais , Anticorpos Antiprotozoários/sangue , Brasil/epidemiologia , Bovinos , Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/transmissão , Variação Genética , Genótipo , Saúde Global , Humanos , Carne/parasitologia , Leite/parasitologia , Estudos Soroepidemiológicos , Toxoplasma/classificação , Toxoplasma/genética , Toxoplasma/imunologia , Toxoplasmose Animal/diagnóstico , Toxoplasmose Animal/epidemiologia , Toxoplasmose Animal/transmissão , Toxoplasmose Congênita/transmissãoRESUMO
Toxoplasma gondii infections are common in humans and animals worldwide. The present review summarizes worldwide information on the prevalence of clinical and subclinical infections, epidemiology, diagnosis, control, and genetic diversity of T. gondii in sheep in the past decade. There is debate and uncertainty concerning repeat congenital infection as evidenced by finding T. gondii DNA in progeny of chronically infected sheep. However, there is no concrete evidence that T. gondii is the cause of repeated abortions in sheep. Recent data concerning pathogenesis of abortion in acutely infected sheep are reviewed. PCR-RFLP typing of T. gondii DNA derived from viable T. gondii isolates or tissues of infected sheep revealed low genetic diversity in sheep in Europe, Africa, Asia and North America but high diversity in South America. This review will be of interest to biologists, parasitologists, veterinarians, and public health workers.
Assuntos
Doenças dos Ovinos , Toxoplasma/genética , Toxoplasmose Animal , Animais , Infecções Assintomáticas/economia , Infecções Assintomáticas/epidemiologia , Prevalência , Ovinos , Doenças dos Ovinos/diagnóstico , Doenças dos Ovinos/economia , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/prevenção & controle , Carneiro Doméstico , Toxoplasmose Animal/diagnóstico , Toxoplasmose Animal/economia , Toxoplasmose Animal/epidemiologia , Toxoplasmose Animal/prevenção & controleRESUMO
Toxoplasma gondii infections are common in humans and animals worldwide. The present review summarizes worldwide information on the prevalence of clinical and subclinical infections, epidemiology, and genetic diversity of T. gondii in horses, donkeys and other equids in the past decade. This review will be of interest to biologists, parasitologists, veterinarians, and public health workers.
Assuntos
Equidae/parasitologia , Toxoplasmose Animal/parasitologia , Animais , Prevalência , Saúde Pública , Toxoplasma/genética , Toxoplasmose Animal/epidemiologiaRESUMO
Toxoplasma gondii infections are common in humans and animals worldwide. Domestic free-range chickens (Gallus domesticus) are excellent sentinels of environmental contamination with T. gondii oocysts because they feed on the ground. Chickens can be easily infected with T. gondii; however, clinical toxoplasmosis is rare in these hosts. Chickens are comparatively inexpensive and thus are good sentinel animals for T. gondii infections on the farms. Here, the authors reviewed prevalence, the persistence of infection, clinical disease, epidemiology and genetic diversity of T. gondii strains isolated from chickens worldwide for the past decade. Data on phenotypic and molecular characteristics of 794 viable T. gondii strains from chickens are discussed, including new data on T. gondii isolates from chickens in Brazil. This paper will be of interest to biologists, epidemiologists, veterinarians and parasitologists.
Assuntos
Galinhas/parasitologia , Toxoplasma , Toxoplasmose Animal/epidemiologia , Animais , Antígenos de Protozoários/sangue , Brasil/epidemiologia , Fezes/parasitologia , Genes de Protozoários , Variação Genética , Oocistos/isolamento & purificação , Patologia Molecular/métodos , Reação em Cadeia da Polimerase/veterinária , Polimorfismo de Fragmento de Restrição , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/patologia , Prevalência , Estudos Soroepidemiológicos , Testes Sorológicos/veterinária , Toxoplasma/genética , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/patologiaRESUMO
Toxoplasma gondii infections are common in humans and animals worldwide. Toxoplasmosis in goats continues to be of public health and economic concern. The present review summarizes worldwide information on the prevalence of clinical and subclinical infections, epidemiology, diagnosis, treatment, and genetic diversity of T. gondii in goats in the past decade. There is debate and uncertainty regarding excretion of T. gondii in milk and the ingestion of raw milk as sources of T. gondii infection for humans. Toxoplasmosis can cause abortion and deaths even in adult goats. PCR-RFLP typing of T. gondii DNA derived from viable T. gondii isolates has revealed genetic diversity in goats in North and South America. The significance of T. gondii isolates in domestic goats usually associated with wildlife in USA is discussed. This review will be of interest to biologists, parasitologists, veterinarians, and public health workers.
Assuntos
Doenças das Cabras/parasitologia , Saúde Pública , Toxoplasmose Animal/economia , Animais , Anticorpos Antiprotozoários , Doenças das Cabras/economia , Doenças das Cabras/epidemiologia , Cabras , Humanos , Leite , Reação em Cadeia da Polimerase/veterinária , Polimorfismo de Fragmento de Restrição , Prevalência , Estudos Soroepidemiológicos , Toxoplasma/genética , Toxoplasmose Animal/epidemiologia , ZoonosesRESUMO
Toxoplasma gondii infections are common in humans and animals worldwide. Toxoplasmosis continues to be of public health concern. Cats (domestic and wild felids) are the most important host in the epidemiology of toxoplasmosis because they are the only species that can excrete the environmentally resistant oocysts in feces. Cats can excrete millions of oocysts and a single cat can spread infection to many hosts. The present paper summarizes information on prevalence, persistence of infection, clinical signs, and diagnosis of T. gondii infections in domestic and wild cats for the past decade. Special emphasis is paid to genetic diversity of T. gondii isolates from cats. Review of literature indicates that a unique genotype (ToxoDB genotype #9 or Chinese 1) is widely prevalent in cats in China and it has been epidemiologically linked to outbreaks of clinical toxoplasmosis in pigs and deaths in humans in China; this genotype has rarely been detected in other countries. This review will be of interest to biologists, parasitologists, veterinarians, and public health workers.
Assuntos
Doenças do Gato , Genótipo , Toxoplasma/fisiologia , Animais , Infecções Assintomáticas/epidemiologia , Doenças do Gato/diagnóstico , Doenças do Gato/tratamento farmacológico , Doenças do Gato/epidemiologia , Gatos , China/epidemiologia , Epidemiologia Molecular , Prevalência , Toxoplasma/genética , Toxoplasmose Animal/diagnóstico , Toxoplasmose Animal/tratamento farmacológico , Toxoplasmose Animal/epidemiologiaRESUMO
To assess the role of white-tailed deer (Odocoileus virginianus, WTD) in the epidemiology of toxoplasmosis, we conducted a national survey of WTD across the USA for Toxoplasma gondii infection. To do this, we combined serology with parasite isolation to evaluate the prevalence and genetic diversity of T. gondii in this game species. From October 2012 to March 2019, serum and tissues were collected from 914 WTD across the USA. Serum samples were screened for antibodies to T. gondii, and then the tissues of seropositive WTD were bioassayed in mice. Antibodies were detected in 329 (36%) of 914 WTD tested by the modified agglutination test (positive reaction at 1:25 or higher). Viable T. gondii was isolated from the heart of 36 WTD from 11 states. Three of the 36 isolates were pathogenic but not highly virulent to outbred Swiss Webster mice and all 36 isolates could be propagated further in cell culture and were genotyped. For genotyping, DNA extracted from cell culture-derived tachyzoites was characterized by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using the genetic markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico. Genotyping revealed seven ToxoDB PCR-RFLP genotypes, including 24 isolates for genotype #5 (haplogroup 12), four isolates for #2 (type III, haplogroup 3), three isolates for genotypes #1 (type II, haplogroup 2), two isolates for genotypes #3 (type II, haplogroup 2) and one isolate each for #39, #221 and #224. Genotype #5 was the most frequently isolated, accounting for 66.6% (24 of 36) of the isolates. Combining the 36 isolates from this study with previously reported 69 isolates from WTD, 15 genotypes have been identified. Among these, 50.4% (53/105) isolates belong to genotype #5. Our results indicate moderate genetic diversity of T. gondii in WTD. The results also indicate that undercooked venison should not be consumed by humans or fed to cats.
Assuntos
Cervos/parasitologia , Reservatórios de Doenças/veterinária , Parasitologia de Alimentos/estatística & dados numéricos , Variação Genética , Carne/parasitologia , Toxoplasma/genética , Animais , Culinária , Reservatórios de Doenças/parasitologia , Feminino , Masculino , Estados UnidosRESUMO
Feral swine are known reservoirs of various pathogens, including Toxoplasma gondii. Here, we report the first national survey of viable T. gondii in feral swine in the USA. We paired serological surveys with parasite isolation and bioassay to evaluate the prevalence and genetic diversity of these parasites. From 2012-2017, sera and tissues from 1517 feral swine across the USA were collected for the isolation of viable T. gondii. Serum samples were initially screened for antibodies to T. gondii, and then the tissues of seropositive feral swine were bioassayed in mice. Antibodies were detected in 27.7% of feral swine tested by the modified agglutination test (1:25 or higher). Antibody positive rates increased significantly with age, with 10.1% of juveniles, 16.0% of sub-adults and 38.4% of adults testing seropositive. Myocardium (50 g) from 232 seropositive feral swine was digested in pepsin and bioassayed in mice. Viable T. gondii was isolated from 78 feral swine from 21 states. Twelve of the 78 isolates were pathogenic to outbred Swiss Webster mice and 76 of the 78 isolates could be propagated further in cell culture and were genotyped. For genotyping, deoxyribonucleic acid extracted from cell culture-derived tachyzoites was characterized by polymerase chain reaction restriction fragment length polymorphism using the genetic markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico. Genotyping revealed 15 ToxoDB genotypes, including 43 isolates for genotype #5 (haplogroup 12), 11 isolates for #24, four isolates for #2 (haplogroup 3), two isolates for each of genotypes #3 (haplogroup 2), #4 (haplogroup 12), #216, #221, #289 and #297 and one isolate for each of genotypes #1 (haplogroup 2), #39, #66, #260, #261 and #299. Genotype #5 was the most frequently isolated, accounted for 57% (43/76) of the isolates, followed by #24, accounted for 14% (11/76). Genotypes #260, #289, #297 and #299 are new types. Genotype #289 was highly virulent to mice and originated from feral swine collected in Louisiana on the same day at the same location. Genotype #216 was previously demonstrated to be highly virulent to mice. Our results indicate moderate genetic diversity of T. gondii in feral swine in the USA, with the genotype #5 (haplogroup 12) dominant in the continental USA, whereas genotype #24 (10/14) was dominant in Hawaii, suggesting different population structures of the parasites among the two distinct geographical locations.
Assuntos
Variação Genética , Genótipo , Doenças dos Suínos/epidemiologia , Toxoplasma/genética , Toxoplasmose Animal/epidemiologia , Animais , Animais Selvagens , Suínos , Doenças dos Suínos/parasitologia , Doenças dos Suínos/transmissão , Toxoplasma/isolamento & purificação , Toxoplasma/patogenicidade , Toxoplasmose Animal/parasitologia , Toxoplasmose Animal/transmissão , Estados Unidos/epidemiologia , Virulência/genéticaRESUMO
Toxoplasma gondii infections are prevalent in most warm-blooded animals worldwide. During the 2018 November hunting season in Pennsylvania, fresh (unfixed, not frozen) samples obtained from 99 harvested elk (Cervus canadensis) were tested for T. gondii infection. Antibodies to T. gondii were detected in 69 of 99 (69.7%) elk tested by the modified agglutination test (MAT, 1:25 cut-off). Tongues and hearts from 16 elk with high MAT titers (>1:200) were bioassayed for T. gondii by inoculation in outbred Swiss Webster (SW) and interferon-gamma gene knockout (KO) mice. Viable T. gondii was isolated from tongues of 2 elk with MAT titers of 1:200 and 1:3,200. Toxoplasma gondii from both isolates were successfully propagated in cell culture. Genetic typing on DNA extracted from culture-derived tachyzoites using the PCR restriction fragment length polymorphism with 10 genetic markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) revealed that both isolates belonged to ToxoDB PCR-RFLP genotype #5 that is widely prevalent in wildlife in the United States. Our results suggest that elk may clear T. gondii organisms from their tissues.
Assuntos
Cervos/parasitologia , Toxoplasma/imunologia , Toxoplasmose Animal/epidemiologia , Testes de Aglutinação/veterinária , Animais , Anticorpos Antiprotozoários/sangue , Bioensaio/veterinária , DNA de Protozoário/química , DNA de Protozoário/isolamento & purificação , Feminino , Técnicas de Genotipagem/veterinária , Coração/parasitologia , Masculino , Camundongos , Camundongos Knockout , Pennsylvania/epidemiologia , Reação em Cadeia da Polimerase/veterinária , Polimorfismo de Fragmento de Restrição , Estudos Soroepidemiológicos , Língua/parasitologia , Toxoplasma/genética , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/parasitologia , Toxoplasmose Animal/transmissãoRESUMO
Cats are important in the epidemiology of Toxoplasma gondii infection because they are the only hosts that can excrete the environmentally resistant oocysts in the environment. Although exposure is common (approximately 30% of cats in the USA), clinical toxoplasmosis is relatively rare. Here, we report overwhelming disseminated toxoplasmosis in two litter mate 8-week-old kittens, thought to have acquired toxoplasmosis postnatally. Five domestic shorthair kittens, approximately 2-3â¯weeks of age, and the queen were found in upstate New York by a rescue group in spring of 2018. The kittens and queen were placed in a foster home for approximately 4-5â¯weeks and then transferred to a shelter. Two kittens died unexpectedly following a short illness. Postmortem examination of the two deceased kittens revealed overwhelming toxoplasmosis and the presence of entero-epithelial stages in small intestine, suggestive of recent ingestion of infected tissues. Antibodies to T. gondii were found in the deceased kittens and the queen but not in the three asymptomatic littermate kittens. No obvious cause of immunosuppression was demonstrated. Genetic typing of T. gondii from DNA extracted from liver and lungs of both kittens revealed Toxo DB #4 genotype, commonly found in wildlife. Owners and veterinarians should be aware of dangers of feeding raw meat to cats and contact with infected cat feces. Procedures to safely handle T. gondii infected feces in hospital setting are outlined.
Assuntos
Doenças do Gato/parasitologia , Gatos/parasitologia , Genótipo , Toxoplasma/genética , Toxoplasma/patogenicidade , Toxoplasmose Animal/mortalidade , Animais , Animais Selvagens/parasitologia , Fezes/parasitologia , Feminino , Tamanho da Ninhada de Vivíparos , Fígado/parasitologia , Pulmão/parasitologia , Masculino , Carne/parasitologia , Gravidez , Alimentos Crus/parasitologia , Toxoplasmose Animal/diagnósticoRESUMO
Toxoplasmosis is a worldwide zoonosis. The ingestion of uncooked/undercooked meat and consumption of water contaminated with Toxoplasma gondii oocysts excreted by felids are the main modes of transmission of this parasite. T. gondii has been reported in multiple cervid species; however, little is known of the parasite in North American elk (Cervus canadensis). In the present study, antibodies to T. gondii were detected in serum of wild elk from Pennsylvania collected during 2013-2016 by the modified agglutination test (MAT, cut-off 1:25); 221 of 317 (69.7%) had MAT titers of 1:25 in 19, 1:50 in 28, 1:100 in 34, and 1:200 or higher in 140. Thus most (44.1%) elk had relatively high titers. Seroprevalence was slightly higher in males (76.9%) than females (67.5%, not statistically significant, Chi-square tests, P<0.0001) and was higher in adults (76.5%) than yearlings (46.4%, Odds ratio 3.82; 95% CL 1.72-8.47; P=0.001) or calves (21.7%, Odds ratio 12.58; 95% CL 4.51-35.10; P<0.0001). Annual seroprevalence was relatively stable throughout the period tested and ranged from 66.6% to 72.2%. Of the 101 elk harvested in 2016, hearts were bioassayed from 20 elk and tongues were bioassayed from 56; all tongue samples were negative. Viable T. gondii was isolated from hearts of two female elk, one of these was a seronegative adult and the other was a calf with no serum available for testing. Both T. gondii isolates were cultivated in cell culture and DNA derived from tachyzoites was characterized using the PCR-RFLP markers including SAG1, SAG2 (5'- 3'SAG2 and altSAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. One isolate belongs to ToxoDB PCR-RFLP genotype #2 and the other is genotype #5. Both genotypes are frequently identified in animals in North America.