Pumpkin seeds, lemongrass essential oil and ripleaf leaves as feed additives for Ascaridia galli infected laying hens.

The present study was performed to evaluate the in vivo efficiency of Curcurbita pepo (pumpkin) seeds, Cymbopogon citratus (lemongrass) essential oil and Plantago lanceolata (ripleaf) leaves against helminth infections in laying hens. In the first experiment, 75 Lohmann LSL Classic hens naturally infected with Ascaridia galli were assigned to groups of five; groups were randomly assigned to one of three treatments with five replicates each (untreated control; lemongrass oil: 1 g/bird/day; pumpkin seeds: 10 g/bird/day). Feed consumption and egg production were continuously recorded, individual faecal egg counts were determined weekly, and E. coli and Lactobacillus spp. three times during the experimental period of 29 days. After slaughter, intestinal worms were counted and sexed. Pumpkin improved feed conversion as compared to the control (p = 0.008) and to lemongrass (p = 0.021); no treatment effect on any other parameter was found. In the second experiment, 75 LSL pullets were artificially infected with 3 × 200 A. galli eggs, randomly divided into groups of five and assigned to one of three treatments (untreated control, lemongrass oil: 1 g/bird/day; ripleaf: 5% of ration). After 109 days of sampling as described above, hens were slaughtered and worm burdens determined. Performance of the animals did not change regardless of the treatment and none of the treatments resulted in changes of the microbiological and parasitological parameters. In conclusion, with the exception of improved feed conversion in the pumpkin group, no positive nor negative effects of the additives on performance, parasitological and microbiological parameters of naturally and artificially A. galli infected laying hens were observed.

In vitro efficacies of oils, silicas and plant preparations against the poultry red mite Dermanyssus gallinae.

The aim of this study was to test the effectiveness of physically acting substances (oils and silicas) and plant preparations for the control of the poultry red mite Dermanyssus gallinae (De Geer 1778). Reproduction and survival of fed D. gallinae females were evaluated in vitro for a total of 168 h using the "area under the survival curve" (AUC) to compare survival of the mites between treatments. Four oils (two plant oils, one petroleum spray oil and diesel), one soap, three silicas (one synthetic amorphous silica, one diatomaceous earth (DE) and one DE with 2% pyrethrum extract) and seven plant preparations (derived from Chrysanthemum cineariaefolium, Allium sativum, Tanacetum vulgare, Yucca schidigera, Quillaja saponaria, Dryopteris filix-mas, and Thuja occidentalis) were tested at various concentrations. All the oils, diesel and soap significantly reduced D. gallinae survival. All silicas tested inhibited reproduction. DE significantly reduced mite survival, but amorphous silica was less effective in vitro. Except for pure A. sativum juice and the highest concentration of C. cineariaefolium extract, the plant preparations tested resulted in statistically insignificant control of D. gallinae.