RESUMO
PURPOSE: Visualization of the implanted mesh after a pelvic floor repair surgery is important for evaluating mesh-related complications. We made an X-ray-detectable mesh and studied the histocompatibility and toxicity of it. METHODS: A thin barium sulfate thread was weaved on a traditional polypropylene mesh to make it X-ray detectable. The cytotoxicity of the mesh was tested by the MTT assay on L929 cell line. The histocompatibility and toxicity of mesh were evaluated in rabbits. Meshes were first implanted intraperitoneally. On postoperative day 7, bloods were tested to estimate the acute toxicity of meshes. After 6 months, rabbits were sacrificed and local inflammatory reaction and tissue regeneration at implantation sites were estimated by the HE stain and Masson stain. In addition, CT scans were performed after surgeries to display the location and shape of implanted meshes. RESULTS: Compared to the polypropylene mesh group, no significant difference was observed in the X-ray-detectable mesh group on both in vitro cytotoxicity and in vivo acute and chronic toxicity. The amounts of extra cellular matrix between two groups did not differ. Through CT scan and 3D remodeling, the barium sulfate thread clearly revealed the position and shape of the X-ray-detectable mesh, whereas the traditional mesh was invisible under CT scan. CONCLUSION: Adding a thin barium sulfate thread on the polypropylene mesh does not change its histocompatibility or toxicity in rabbit model. The barium sulfate thread can effectively show the location and shape of implanted mesh under CT scan.
