Development of a sensitive electrochemical method to determine amitraz based on perylene tetracarboxylic acid/mesoporous carbon/Nafion@SPCEs.

A cutting-edge electrochemical method is presented for precise quantification of amitraz (AMZ), a commonly used acaricide in veterinary medicine and agriculture. Leveraging a lab-made screen-printed carbon electrode modified with a synergistic blend of perylene tetracarboxylic acid (PTCA), mesoporous carbon (MC), and Nafion, the sensor's sensitivity was significantly improved. Fine-tuning of PTCA, MC, and Nafion ratios, alongside optimization of the pH of the supporting electrolyte and accumulation time, resulted in remarkable sensitivity enhancements. The sensor exhibited a linear response within the concentration range 0.01 to 0.70 µg mL-1, boasting an exceptionally low limit of detection of 0.002 µg mL-1 and a limit of quantification of 0.10 µg mL-1, surpassing maximum residue levels permitted in honey, tomato, and longan samples. Validation with real samples demonstrated high recoveries ranging from 80.8 to 104.8%, with a relative standard deviation below 10%, affirming the method's robustness and precision. The modified PTCA/MC/Nafion@SPCE-based electrochemical sensor not only offers superior sensitivity but also simplicity and cost-effectiveness, making it a pivotal tool for accurate AMZ detection in food samples. Furthermore, beyond the scope of this study, the sensor presents promising prospects for wider application across various electrochemical analytical fields, thereby significantly contributing to food safety and advancing agricultural practices.

A novel and portable electrochemical sensor for 5-hydroxymethylfurfural detection using silver microdendrite electrodeposited paper-based electrode.

A portable paper-based electrochemical sensor has been developed to determine 5-hydroxymethylfurfural (5-HMF). A screen-printed carbon electrode (SPCE) was facilely fabricated for the first time on poster paper which showed a very satisfactory electrochemical response. The analytical performance of the electrode was enhanced by electrochemical deposition of silver microdendrites (AgMDs). The cathodic peak of 5-HMF occurred at approximately -1.48 V, lower than that obtained from the bare poster-SPCE. Moreover, the modified electrode showed a higher current response than the bare electrode, revealing that the AgMDs not only exhibited highlighted electrocatalytic features but also improved the electrical conductivity and increased the electrode surface area. Afterward, some influencing conditions were optimized, including scan rate and the number of scan cycles for AgMD deposition, pH, temperature, and square wave voltammetric parameters. Under the optimal conditions, the analytical characteristics of the proposed sensor were evaluated. The cathodic peak current increased linearly according to 5-HMF concentration over the range of 3-100 ppm, and the detection limit was 1.0 ppm. This low-cost, disposable electrochemical sensor provided environmentally friendly, simple and rapid detection, acceptable precision, good stability, and high selectivity. Additionally, this method can be applied to quantify 5-HMF in honey samples with satisfying accuracy.

An Environmentally Friendly Flow Injection-Gas Diffusion System Using Roselle (Hibiscus sabdariffa L.) Extract as Natural Reagent for the Photometric Determination of Sulfite in Wines.

In this work, a green and simpler method for photometric determination of sulfite based on a flow injection-gas diffusion (FI-GD) system using a natural reagent extracted from roselle (Hibiscus sabdariffa L.) was proposed. Despite the fact that the employed reaction is not selective to sulfite, its sensitivity is high, and the selectivity can be improved by coupling a GD unit to the FI system. The method involves monitoring a decrease in absorbance of the reagent solution that is used as an acceptor solution. When a standard solution or sample solution was injected into an acidic donor stream, the liberated sulfur dioxide diffuses through a gas-permeable membrane of the GD unit into the acceptor solution, causing color fading of the reagent. A linear analytical curve in the range of 5-100 mg L-1 was obtained with a detection limit of 2 mg·L-1. Relative standard deviations of 0.9%, 0.6%, and 0.6% were obtained for the determination of 30, 70, and 100 mg·L-1 SO3 2- (n = 11). The developed method was applied to wine samples, giving results that agreed with those obtained with the Ripper titrimetric method. The proposed method offers advantages of simplicity, cost-effectiveness, and being environmentally friendly such as reduced chemical consumption and less waste generation.

A novel flow injection amperometric sensor based on carbon black and graphene oxide modified screen-printed carbon electrode for highly sensitive determination of uric acid.

A simple, rapid, and cost-effective flow injection amperometric (FI-Amp) sensor for sensitive determination of uric acid (UA) was developed based on a new combination of carbon black (CB) and graphene oxide (GO) modified screen-printed carbon electrode (SPCE). The CB-GO nanocomposites were simply synthesized and modified on the working electrode surface to increase electrode conductivity and enhance the sensitivity of UA determination via the electrocatalytic activity toward UA oxidation. The morphologies and electrochemical properties of the synthesized nanomaterials were investigated through scanning electron microscopy (SEM), transmission electron microscopy (TEM), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV). The modified electrode was incorporated with FI-Amp to improve UA detection's sensitivity, stability, and automation. Some parameters affecting sensitivity were optimized, including pH of the electrolyte solution, applied potential, amount of CB-GO suspension, flow rate, injection volume, and reaction coil length. Using an applied potential of +0.35 V (vs Ag/AgCl), the anodic current was linearly proportional to UA concentration over the range of 0.05-2000 µM with a detection limit of 0.01 µM (3 S/N). Besides, the developed method provides a sample throughput of 25 injections h-1, excellent sensitivity (0.0191 µA/µM), selectivity, repeatability (RSD 3.1%, n = 7), and stability (RSD 1.08%, n = 50). The proposed system can tolerate potential interferences commonly found in human urine. Furthermore, a good correlation coefficient between the results obtained from the FI-Amp sensor and a hospital laboratory implies that the proposed system is accurate and can be utilized for UA detection in urine samples.

Sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin G.

Sequential injection (SI) system incorporated with amperometric immunosensor was developed for sensitive determination of human immunoglobulin G (HIgG). A cost effective label-free immunosensor was fabricated by immobilizing anti-HIgG on a graphene oxide (GO) modified screen-printed carbon electrode (SPCE). The developed electrode was characterized by cyclic voltammetry(CV), scanning electron microscope(SEM), and energy dispersive spectroscopy(EDS) which confirmed the selective immunointeraction of HIgG to the anti-HIgG on the electrode, thus reduced the amperometric current of [Fe(CN)6]3-/4- redox probe. The sensing electrode was placed in a designed electrochemical flow cell of SI system, where the redox probe was propelled through and the currents before and after the immunointeraction occurred were measured amperometrically by using a simple home-made amperometer. Under the optimum condition: flow rate of 2mLmin-1, applied potential of +350mV, [Fe(CN)6]3-/4- concentration of 10mM and 10min of incubation time, a linear calibration in the range of 2-100ngmL-1 was achieved, with detection limit of 1.70ngmL-1. The proposed system provided good repeatability and reproducibility and the application for urine sample analysis was demonstrated.

Flow injection amperometric sensor with a carbon nanotube modified screen printed electrode for determination of hydroquinone.

Flow injection amperometric (FI-Amp) sensor was developed for sensitive and selective determination of hydroquinone. A simple screen printed carbon electrode (SPCE) was modified with various nanomaterials for improvement of sensitivity on the determination of quinone. As a result, the appropriate sensitivity is obtained from the SPCE modified with carbon nanotube (CNT) which indicated that CNT contributed to the transfer of electron to quinone. The reproducibility (n=9) and repeatability (n=111) of SPCE-CNT were obtained at 4.4% and 3.6%RSD, respectively. The SPCE-CNT electrode and enzymatic column were incorporated to the FI-Amp system to determine hydroquinone. Laccase was immobilized on silica gel using a cross-linking method by glutaraldehyde modification and then packed in the column. The laccase column has high efficiency for catalytic oxidation of hydroquinone to quinone, which further detects by amperometric detection. Parameters affecting response of the proposed sensor, i.e., pH, ionic strength, and temperature have been optimized. The proposed system provided a wide linear range between 1 and 50 µM with detection limit of 0.1 µM. Satisfactory recoveries in the range of 91.2-103.8% were obtained for the analysis of water sample.

Cost-effective flow injection amperometric system with metal nanoparticle loaded carbon nanotube modified screen printed carbon electrode for sensitive determination of hydrogen peroxide.

Various metal nanoparticles (NPs) decorated on carbon nanotube (CNT) was modified on the home-made screen printed carbon electrode (SPCE) in order to enhances sensitivity of hydrogen peroxide (H2O2) determination. The simple casting method was used for the electrode modification. The monometallic and bimetallic NPs modified electrodes were investigated for their electrochemical properties for H2O2 reduction. The Pd-CNT/SPCE is appropriated to measure the H2O2 reduction at a potential of -0.3 V, then this modified electrode was incorporated with a home-made flow through cell and applied in a simple flow injection amperometry (FI-Amp). Some parameters influencing the resulted modified electrode and the FI-Amp system were studied. The proposed detection system was able to detect H2O2 in the range of 0.1-1.0 mM, with detection limit of 20 µM. Relative standard deviation for 100 replicated injections of 0.6 mM H2O2 was 2.3%. The reproducibility of 6 electrodes preparing in 3 different lots was 8.2%. It was demonstrated for determination of H2O2 in disinfectant, hair colorant and milk samples. Recoveries in the range of 90-109% were observed. The developed system provided high stability, good repeatability, high sample throughput and low reagent consumption.