Evaluation of the immune response of layer chickens to Newcastle disease virus vaccines using the new vaccination regimens.

BACKGROUND: The study aimed to evaluate the immunological response of layer chickens to live Newcastle disease virus vaccine using a newly developed vaccine schedule administered via the ocular route, as well as assess the persistence of passive antibodies in layer chickens and the effectiveness of protection against strains of the virus. METHODS: A total of 140-day-old Lohmann Brown chicks were randomly divided into seven groups, 20 chicks each. Groups 1-3 received a single eye instillation of the vaccine at ages 5, 26 and 54 days, respectively, whereas groups 4-6 received a double eye instillation. Group 7 served as non-vaccinated control group. Ten days after immunization, samples were taken from hens that had received the vaccine at ages 15, 36 and 64, as well as from control chickens that had not received the vaccine at ages 5, 15, 21 and 31. RESULTS: A total of 10 serum samples from all chickens exhibited protective antibodies, and booster doses resulted in the highest haemagglutination inhibition titre. No significant change in antibody production was observed among layer hens (p > 0.05). The study found that the La Sota (GMT ± SD: 6.71 ± 4.96), La Sota (GMT ± SD: 8.00 ± 0.00) and thermostable I2 (GMT ± SD: 7.60 ± 6.02), vaccination schedules provided the maximum immune response in single eye instillation, whereas the HB1 (GMT ± SD: 7.11 ± 4.77), La Sota (GMT ± SD: 7.83 ± 5.76) and La Sota (GMT ± SD: 7.60 ± 6.02), combination was the second-best vaccination schedule in double eye instillation. Furthermore, maternally-derived antibodies were maintained up to 31 days of age, indicating the level of passive immunity prior to vaccination. Characteristic lesions, such as edematous and diphtheria mucosal membranes of the trachea, along with petechial and necrotic haemorrhages of the proventriculus, were observed during the necropsy of the birds that died from the challenged virus. CONCLUSION: This study suggests that subsequent live virus vaccine by ocular route immunization is required to effectively protect against velogenic viscerotropic Newcastle disease infection. The results also highlight the importance of developing effective vaccination schedules and routes to enhance immunity against ND in layer chickens.

Seroprevalence of Coxiella burnetii, Leptospira interrogans serovar hardjo, and Brucella species and associated reproductive disorders in cattle in southwest Ethiopia.

In Ethiopia, Coxiella burnetii, Leptospira Hardjo, and Brucella spp are recognized as the primary factors contributing to cattle reproductive issues. A cross-sectional study was conducted in southwest Ethiopia from October 2020 to October 2021 to assess the risk of reproductive disorders associated with L. Hardjo, Coxiella burnetii, and Brucella spp. Moreover, the study aimed to identify the factors associated with reproductive disorders. Using an indirect ELISA, antibodies against these pathogens were observed in serum samples collected from 461 cattle. We employed multivariable random effect logistic regression analysis to identify potential risk factors associated with reproductive disorders in cattle. The study areas showed a prevalence of 25.16 % (95 % CI: 21.20-29.12) for cattle reproductive disorders. The presence of Leptospira Hardjo (OR = 2.9, 95 % CI: 1.17-4.02) and Coxiella burnetii (OR = 3.0, 1.49-5.94) antibodies was associated to the occurrence of cattle reproductive disorders. Seropositivity to pathogens B. abortus, C. burnetii, and L. Hardjo, along with co-infection of all three, showed association with cattle abortion. The presence of L. Hardjo seropositivity and co-infection with C. burnetii were related to dystocia in cattle. Cattle with retained fetal membranes were associated with co-infection seropositivity to these pathogens. Additionally, B. abortus seropositivity was linked to cases of repeated breeding in cattle. Age, breeding practices, and dog access to cattle showed associations with reproductive disorders, with odds ratios of 2.3 (95 % CI: 2.03-4.69), 2.9 (95 % CI: 1.83-4.82), and 6.5 (95 % CI: 1.04-2.53) respectively. This research indicates that Brucella abortus, Coxiella burnetii, and Leptospira Hardjo, which are responsible for severe zoonotic diseases, have a substantial negative impact on cattle production by causing reproductive disorders. To address the transmission of these diseases, it is essential to implement effective mitigation strategies and enhance public awareness. Additional investigation is necessary to identify and understand the factors contributing to cattle reproductive disorders in the specified area.

Adoption of veterinary vaccines, determining factors, and barriers in Southwest Ethiopia: Implications for livestock health and disease management strategies.

In Ethiopia, the use of veterinary vaccines to control animal diseases is an effective strategy. A study conducted in Southwest Ethiopia from October 2020 to October 2021 aimed to determine the adoption level of veterinary vaccines and factors affecting their use. The study used multistage random sampling to select districts and interviewed 476 farmers who had either adopted or not adopted the vaccines. The study found that certain diseases should be prioritized for vaccination to safeguard the health of cattle, sheep, goats, and poultry. These include anthrax (19.12 %), blackleg (17.65 %), foot and mouth disease (10.50 %), and lumpy skin disease (8.82 %) in cattle, and pasteurellosis (18.07 %), contagious caprine pleuropneumonia (15.97 %), peste des petits ruminants (14.15 %), and Orf (13.45 %) in sheep and goats. Newcastle disease (21.85 %), infectious bursal disease (19.33 %), and coccidiosis (17.02 %) were identified as high-priority diseases for flock health. Overall, 30.7 % of farmers were adopters of veterinary vaccines, while 69.3 % were non-adopters. The study identified several factors that influence the likelihood of adopting veterinary vaccines, including breed type (OR = 9.1, p < 0.0001), production size (OR = 9.7, p < 0.0001), production type (OR = 2.7, p < 0.0001), and farm location (OR = 9.8, p = 0.001). Common barriers to vaccination included a lack of disease knowledge, high vaccine costs, limited vaccine availability, and administration difficulties. Insights from the study can guide strategies for promoting veterinary vaccine adoption in Ethiopia. Stakeholders should pay attention to these findings since vaccine use is crucial for controlling animal diseases, enhancing animal health, and preventing economic losses. Further research is needed to investigate factors affecting enhanced veterinary vaccine adoption.

Using participatory epidemiology to investigate the causes of cattle abortion in Southwest Ethiopia.

Cattle abortion, stemming from both infectious and non-infectious causes, lead to notable financial setbacks in the cattle industry. Between October 2020 and October 2021, an epidemiological investigation took place in Southwest Ethiopia. The objective was to determine the magnitude and seasonal occurrence of the presumed causes of cattle abortion. Information for this research was collected through 30 group discussions, each involving 8-12 participants. Various participatory epidemiological tools, including semi-structured interviews, pairwise ranking, matrix scoring, proportional piling, and seasonal calendars, were employed in the designated areas. By employing the pairwise ranking approach, the relative significance of presumed causes contributing to cattle abortion was established. The identified major presumed causes of cattle abortion, listed in increasing order of importance, were blackleg, foot-and-mouth disease, pasteurellosis, lumpy skin disease, listeriosis, trypanosomosis, Q fever, leptospirosis, and brucellosis. Participants identified brucellosis (6.1%), leptospirosis (6.0%), and Q-fever (5.7%) as the primary presumed causes of abortion, determined through proportional piling. Matrix scoring analysis indicated a robust agreement (W = 0.464-0.989; P < 0.001) among different informant groups regarding both the presumed causes of abortion and the associated clinical signs. Brucellosis and Q-fever were perceived to be more prevalent during the dry season, while leptospirosis, listeriosis, and lumpy skin disease were associated with the wet, hot, and rainy seasons. However, Pasteurellosis, blackleg, and physical/mechanical factors were deemed to be consistently encountered causes of abortion throughout the year. The patterns of seasonal occurrence of suspected abortion causes were widely acknowledged across informant groups (W = 0.977-0.863; P < 0.001). Local practices involving herbal remedies and traditional methods were employed by participants to manage cattle abortion. Moreover, the results underscore the necessity for additional laboratory research to pinpoint the exact causes of abortion in the study areas.

Health status of honeybee colonies (Apis mellifera) and disease-associated risk factors in different agroecological zones of Southwest Ethiopia.

A cross-sectional study design was conducted in different agroecological zones of southwest Ethiopia from October 2019 to October 2021. The study aimed to determine the prevalence and associated risk factors for honeybee diseases and pests, as well as the impact of these issues on honeybee colonies and their products. To identify potential risk factors for honeybee disease and pests, a multivariate random effects logistic regression analysis was used. Adult honeybee and brood samples from a total of 384 honeybee colonies were collected and tested using standard laboratory diagnostic methods. The highest prevalence (55.8%) of ants was recorded, followed by wax moths (22.5%) and hive beetles (23.3%). In the current study, the main honeybee diseases observed in the study areas were varroosis (36.5%), bee lice (5.2%), nosemosis (39.6%), amoeba (56%), and chalkbrood (4.5%). However, tracheal mites, sachbrood, and American and European foul brood, were not detected. The agroecological zone (OR = 5.2, 95% CI: 1.75-14.85), type of hive (OR = 2.9, 95% CI: 1.17-17.03), management system (OR = 4.3, 95% CI: 1.23-14.70), and the management of the colony (OR = 3.5, 95% CI: 1.31-9.14) were identified as risk factors for varroosis in these areas. The occurrence of nosemosis in colonies was also influenced by the agroecological zone (OR = 12.2, 95% CI: 3.06-48.54) and colony management (OR = 3.4, 95% CI: 1.59-7.23). The agroecological zone (OR = 10.5, 95% CI: 12.76-22.63) and hive type (OR = 3.0, 95% CI: 1.39-6.36) were the primary risk factors for the occurrence of amoeba in honeybee colonies. However, the occurrence of bee lice (OR = 34.7, 95% CI: 3.96-104.93) and chalkbrood (OR = 4.8, 95% CI: 1.44-13.16) in honeybee colonies was only influenced by the agroecological zone in the study areas. This study demonstrated that losses in honey production in the area are significantly attributed to honeybee disease and pests. Therefore, it is essential to increase public awareness of how honeybee diseases and pests affect honey production and to develop and implement appropriate control measures for these diseases and pests. Furthermore, more studies should be conducted to characterize and isolate other causes of honeybee diseases and pests in various locations.

A Comprehensive Review of the Common Bacterial Infections in Dairy Calves and Advanced Strategies for Health Management.

Dairy farming faces a significant challenge of bacterial infections in dairy calves, which can have detrimental effects on their health and productivity. This review offers a comprehensive overview of the most prevalent bacterial infections in dairy calves, including Escherichia coli, Salmonella typhimurium, Salmonella dublin, Salmonella enterica, Clostridium perfringens, Pasteurella multocida, Listeria monocytogenes, Mycoplasma bovis, and Haemophilus somnus. These pathogens can cause various clinical signs and symptoms, leading to diarrhea, respiratory distress, septicemia, and even mortality. Factors such as management practices, environmental conditions, and herd health influence the incidence and severity of the infections. Efficient management and prevention strategies include good colostrum and nutrient feeding, early detection, appropriate treatment, hygiene practices, and supportive care. Regular health monitoring and diagnostic tests facilitate early detection and intervention. The use of antibiotics should be judicious to prevent antimicrobial resistance and supportive care such as fluid therapy and nutritional support promotes recovery. Diagnostic methods, including immunological tests, culture, polymerase chain reaction (PCR), and serology, aid in the identification of specific pathogens. This review also explores recent advancements in the diagnosis, treatment, and prevention of bacterial infections in dairy calves, providing valuable insights for dairy farmers, veterinarians, and researchers. By synthesizing pertinent scientific literature, this review contributes to the development of effective strategies aimed at mitigating the impact of bacterial infections on the health, welfare, and productivity of young calves. Moreover, more research is required to enhance the understanding of the epidemiology and characterization of bacterial infections in dairy calves.

Eukaryotic Infections in Dairy Calves: Impacts, Diagnosis, and Strategies for Prevention and Control.

Eukaryotic infections are common among dairy calves and can have significant impacts on their health and growth rates. Fungal infections caused by Aspergillus fumigatus, Trichophyton verrucosum, and Candida albicans can cause respiratory diseases, dermatophytosis, and diarrhea, respectively. Protozoan parasites, including Cryptosporidium parvum, Giardia duodenalis, and Eimeria spp., are also common in dairy calves. C. parvum is highly contagious and can cause severe diarrhea and dehydration, while Giardia duodenalis can lead to poor growth and is transmissible to humans through contaminated food or water. Eimeria spp. can cause coccidiosis and lead to reduced growth rates, poor feed conversion, and death. The common helminthic infections in dairy calves include Ostertagia ostertagi, Cooperia spp., Fasciola hepatica, and Strongyloides papillosus. These parasitic infections significantly impact calf health, growth, and dairy industry productivity. Diagnosis of these infections can be made through fecal samples using microscopy or molecular methods. However, diagnosis of the infections can be challenging and requires a combination of clinical signs and laboratory tests such as culture and PCR. Preventing and controlling eukaryotic infections in dairy calves requires several measures. Good hygiene and sanitation practices, proper management strategies, and timely treatment of affected animals are important. It is also necessary to avoid overcrowding and consider vaccination against ringworm. Further research is needed to better understand the epidemiology and characterization of eukaryotic infections in dairy calves, which will help in the development of more effective prevention and control strategies. In general, good hygiene practices, appropriate management strategies, and timely treatment of affected animals are crucial in preventing and controlling the infections, ensuring the health and well-being of dairy calves.

Evaluation of livestock farmers' knowledge, attitudes and practices regarding the use of veterinary vaccines in Southwest Ethiopia.

BACKGROUND: The utilization of veterinary vaccines plays a vital role in preventing and managing animal diseases. However, the success of vaccination programmes relies on livestock farmers' understanding, perspectives and behaviours regarding their application. Therefore, this study aims to evaluate the knowledge, attitudes and practices of livestock farmers in Southwest Ethiopia regarding the use of veterinary vaccines. METHODS: The study followed a cross-sectional design conducted between October 2021 and October 2022. A sample of 476 livestock-owning farmers, including those who raise dairy cattle, beef cattle and poultry, were interviewed from the districts of Shei Bench, Semen Bench, Sheko and Yeki. RESULTS: The findings revealed that participants from Sheko, who had a poultry farm, were college graduates and resided in an urban area, were more likely to have good knowledge of veterinary vaccines. Similarly, participants who were male, from Semen Bench, had a poultry farm and had a high school education, exhibited a positive attitude towards veterinary vaccines. Furthermore, being male, from Semen Bench, having a poultry farm, and having a high school education were associated with increased veterinary vaccine use. CONCLUSION: This study highlights the importance of addressing gender differences and tailoring interventions based on geography and farming conditions in the area. These measures are crucial to improve practices related to veterinary vaccines for enhanced animal health and productivity. Prioritizing education, veterinary services and information dissemination is vital. However, further research and targeted interventions are needed to better comprehend underlying factors and implement effective strategies for diverse communities in different areas.

Epidemiological investigation of Coxiella burnetii in cattle and its association with Ixodid tick infestation in different agro-ecological zones of Southwest Ethiopia.

Coxiella burnetii is a serious zoonotic disease that causes significant economic losses in cattle production, including abortion, stillbirth, infertility, and reduced milk yield. However, little is known about the epidemiology of C. burnetii in Ethiopia. From November 2020 to November 2021, a cross-sectional study was conducted to estimate the seroprevalence and associated risk factors of C. burnetii in cattle in various agro-ecologies of Southwest Ethiopia. Blood samples were collected from 461 cattle, and the serum samples were tested for the presence of C. burnetii antibodies using an indirect ELISA. To identify potential risk factors for C. burnetii seropositivity, a multivariable mixed-effect logistic regression analysis was used. The study found an overall seroprevalence of 8.68% (95% CI: 6.11-11.25) and 13.57% (95% CI: 9.56-17.58) at the animal and herd levels, respectively, in the study areas. The results of the study indicated that C. burnetii infection was a widespread disease in the study areas. C. burnetii seropositivity at the animal level was significantly associated with age (OR = 4.1, 95%CI: 1.47-10.92), herd size (OR = 3.9, 95%CI: 1.21-12.66), management system (OR = 9.7, 95%CI: 1.27-27.25), cattle access to dogs, cats, and mice (OR = 2.5, 95%CI: 1.21-5.28), accessibility of cattle to wild animals (OR = 4.2, 95%CI: 1.01-17.18), presence of ticks on cattle (OR = 2.3, 95%CI: 1.12-4.83), and history of abortion (OR = 3.8, 95%CI: 1.78-8.23). A herd level analysis identified several risk factors for C. burnetii infection, including the management system (OR = 3.8, 95%CI: 1.59-8.98), agro-ecology (OR = 2.8, 95%CI: 1.43-7.21), herd size (OR = 4.3, 95%CI: 1.69-9.76), and accessibility of cattle to dogs, cats, and mice (OR = 2.6, 95%CI: 1.18-3.96). Therefore, it is important to implement appropriate control methods and raise public awareness about C. burnetii zoonotic transmission. Moreover, further studies should be conducted to isolate and characterize C. burnetii as a cause of reproductive problems and in disease reservoirs such as ticks and wildlife in the study areas.

Epidemiology, factors influencing prevalence and level of varroosis infestation (Varroa destructor) in honeybee (Apis mellifera) colonies in different agroecologies of Southwest Ethiopia.

Little information is available on the epidemiology of varroosis caused by Varroa mite, Varroa destructor infestation in Ethiopia, although it is a devastating honeybee disease that results in significant economic losses in beekeeping. Therefore, between October 2021 and October 2022, a cross-sectional study was carried out in different agroecology zones in Southwest Ethiopia to determine the prevalence and associated risk factors for varroosis, as well as the effects of this disease on honeybee colonies and honey production. A multivariate logistic regression analysis was performed to identify possible risk factors for the prevalence of V. destructor. A total of 384 adult honeybee and worker or drone brood samples were collected from honeybee colonies and examined using standard diagnostic techniques in the laboratory. The result shows that the prevalence of V. destructor was found to be 39.3% (95% CI 34.44-44.21) and 43.2% (38.27-48.18) in adult honeybees and brood, respectively. The major risk factors for the prevalence of V. destructor in the study areas included agroecology (OR = 5.2, 95% CI 1.75-14.85), type of hive (OR = 2.9, 95% CI 1.17-17.03), management system (OR = 4.3, 95% CI 1.23-14.70), and colony management (OR = 3.5, 95% CI 1.31-9.14). The lower level of colony infestation in adult bees and brood was measured as 1.97 ± 0.14 and 3.19 ± 0.25, respectively. Season, colony status, colony management, and agroecology were among the determinant factors of the level of varroa mite infestation in adult bees and brood. The results of the study demonstrated that honey production losses are largely attributable to V. destructor infestation. Therefore, it is critical to inform the community about the effects of V. destructor on honey production and develop and implement effective management strategies for this disease. In addition, further research should be done to identify and isolate additional factors that contribute to varroosis in honeybees in different regions.

Coxiellosis in Livestock: Epidemiology, Public Health Significance, and Prevalence of Coxiella burnetii Infection in Ethiopia.

Coxiellosis is a zoonotic disease that is prevalent globally and can pose significant challenges, especially in less developed countries like Ethiopia. Coxiella burnetii is responsible for causing an infection called Q fever in humans and coxiellosis in ruminants. Pneumonia and endocarditis are the only signs that characterize the acute and chronic forms of Q fever, respectively. Ruminants exhibit symptoms such as abortion during the later stages of pregnancy, impaired fertility, perinatal death, premature delivery, and reduced birth weight. C. burnetii infection typically spreads among healthy cattle via tick bites and exposure to infected cattle or their bodily secretions. The primary source of human infection is through the ingestion of contaminated milk and milk products, but transmission through aerosols and dust generated during livestock operations is also common. Cattle, sheep, camels and goats are the primary sources of human infection, and the bacterium can be found in various bodily fluids of infected animals. Several factors, including host characteristics, environmental conditions, and management practices, can potentially affect the occurrence of C. burnetii infection in livestock, such as cattle, camels, sheep, and goats. Coxiellosis is prevalent in Ethiopia's pastoral and mixed cattle management systems, as individuals frequently interact with cattle and are therefore more prone to exposure to the C. burnetii bacterium. Vaccination and biosecurity measures are effective techniques for managing C. burnetii infection. Therefore, it is crucial to implement appropriate mitigation strategies, raise awareness about the spread of C. burnetii infection, and conduct further studies on C. burnetii infection in high-risk groups.

Herd and animal level seroprevalence and associated risk factors of bovine brucellosis in different agro-ecologies of southwest Ethiopia.

Brucellosis is a zoonotic infection that commonly affects cattle in Ethiopia, causing significant negative economic impact. A cross-sectional study was carried out between November 2020 and November 2021 in southwest Ethiopia to determine the seroprevalence of brucellosis and its associated risk factors in cattle herds. Blood samples were taken from 461 randomly selected cattle to test for the presence of Brucella antibodies using the Rose Bengal Plate test, with positive serum confirmed through the complement fixation test. A multivariable random effect logistic regression analysis was used to identify potential risk factors for Brucella seropositivity. The study found 7.14% (95% CI: 4.44-9.01) seroprevalence at the animal level and 12.23% (95% CI: 6.52-16.05) at the herd level based on the complement fixation test. Age (OR = 6.9, 95%CI: 1.83-15.97), herd size (OR = 3.66, 95%CI: 1.39-9.61), introducing new animals (OR = 2.72, 95%CI: 1.17-6.29), management system (OR = 12.2, 95%CI: 1.53-26.80), species composition (OR = 4.24, 95%CI: 1.51-11.91), and abortion (OR = 7.1, 95%CI: 1.93-15.39) were found to be associated with Brucella seropositivity. The analysis also revealed two risk factors for Brucella infection at the herd level, including herd size (OR = 3.4, 95% CI: 1.05-10.68) and species composition (OR = 3.1, 95% CI: 1.20-7.88). The presence of Brucella antibodies in cattle highlights the need for increased awareness and measures to mitigate the identified risk factors of the disease to prevent its spread. Furthermore, further studies are necessary to investigate the zoonotic transmission of brucellosis to humans and its role in cattle reproduction disorders in the study area.

Neglected zoonotic bacteria causes and associated risk factors of cattle abortion in different agro-ecological zones of southwest Ethiopia.

Three zoonotic bacteria, Coxiella burnetii, Leptospira spp, and Brucella spp, cause cattle abortion and significant losses in the livestock sector. However, these infections were neglected diseases in Ethiopia. Between October 2020 and October 2021, a cross-sectional study was carried out in southwest Ethiopia to determine the prevalence of abortion and identify the major zoonotic bacterial causes and risk factors that contribute to abortion. Using an indirect ELISA, antibodies to Brucella abortus, Leptospira Hardjo, and Coxiella burnetii were detected in serum samples taken from 461 cattle. Potential risk factors for cattle abortion were found using a multivariable random effect logistic regression analysis. The prevalence of cattle abortion was found to be 22.56 % (95 % CI: 18.74-26.38) in the study areas. Leptospira Hardjo (OR=1.8, 95% CI: 1.04-3.00), Coxiella burnetii (OR=2.7, 95 % CI: 1.26-5.62), and Brucella abortus (OR=9.8, 95 % CI: 1.27-17.15) were all associated with the occurrence of abortion in cattle, as were their co-infections (OR=8.1, 95 % CI: 1.28-39.33). Other risk factors for cattle abortion included breed (OR=16.7, 95 % CI: 2.20-26.77), herd size (OR=2.5, 95 % CI: 1.16-4.68), breeding method (OR=2.3, 95 % CI: 1.33-3.90), and contact with dogs (OR=2.2, 95 % CI: 1.34-3.53). According to the findings of this study, Brucella abortus, Coxiella burnetii, and Leptospira Hardjo are serious zoonoses that cause significant production loss in cattle due to abortion. To prevent transmission of these zoonotic diseases, it is crucial to implement effective control measures and increase public awareness. The causes of cattle abortion in the study areas should also be isolated and characterized.

A participatory epidemiological investigation of causes of cattle abortion in Jimma zone, Ethiopia.

A participatory epidemiological study was conducted with cattle keepers in Jimma zone, Ethiopia, between October 2018 and October 2019 to identify the causes of abortion in cattle. Data collection involved 20 group discussions (each comprising 8-12 people) in 10 peasant associations. Methods used in group discussions included semi-structured interviews, pairwise ranking, matrix scoring, proportional piling, and seasonal calendar. The result of pairwise ranking identified brucellosis, leptospirosis, listeriosis, trypanosomosis, and Foot and mouth disease (FMD) in decreasing order as the most important causes of abortion in cattle. Mechanical or physical agents were also identified as less important non-infectious causes of cattle abortion in study areas. A very strong agreement (W = 0.880; P < 0.001) was observed among informant groups in pairwise ranking as to the most important cause of cattle abortion in study areas. Proportional piling showed that brucellosis was responsible for the highest proportion of abortions (39.9%) followed by leptospirosis (22.5%) and listeriosis (16.3%). A lesser proportion of abortion was attributed to trypanosomosis and FMD which comprise 11.6% and 9.7%, respectively. Matrix scoring showed strong agreement (W = 0.572 to 0.898; p < 0.001) concerning causes of abortion and its clinical signs between informant groups. According to the discussants, brucellosis and FMD tend to occur more frequently in the winter and spring seasons whereas listeriosis and trypanosomosis occurred frequently in the summer and autumn seasons, respectively. Strong agreement was observed among informant groups about the seasonal pattern of occurrence causes of abortion (W = 0.525-0.794; P < 0.001). Participants used medicinal plants and other traditional practices to manage cattle abortion in their areas. Farmers' knowledge should be incorporated to investigate health problems of unknown causes, designing, and implementing the intervention program in the areas.

Epidemiological investigation of bovine trypanosomosis and distribution of vectors in Jimma zone, Ethiopia.

Trypanosomosis is highly reliant on the distribution of vectors responsible for transmission. A cross-sectional study was conducted to determine the prevalence and associated risk factors of bovine trypanosomosis as well as the distribution of vectors in the Jimma zone, Ethiopia. Blood samples from a total of 2088 cattle were collected and tested using a buffy coat and Giemsa techniques. An overall 13.36% prevalence of trypanosomosis was recorded in study areas. The highest proportion of the infections was caused by T. vivax (44.80%) followed by T. congolense (36.92%) and mixed infection (18.28%) of both species. The study also revealed that trypanosomosis was associated with anemia as the mean PCV was significantly lower among trypanosome-infected animals (20.34 ± 4.39) than non-infected ones (27.98 ± 3.68). Moreover, anemia was more pronounced with T. congolense infection (19.54 ± 3.22) than T. vivax (21.07 ± 3.96) and mixed infection of both species (20.16 ± 2.71). This study identified age, body condition, and agro-ecology as risk factors for the occurrence of trypanosomosis in cattle. Vector survey was conducted by deploying 377 mono-pyramidal traps in selected districts. Accordingly, Glossina species and other biting flies (Stomoxys and Tabanus) were identified with an apparent density of 5.27 and 1.74 fly/trap/day, respectively. Moreover, a higher 4.49 fly/trap/day of G. tachinoides than G. morsitans submorsitans (0.79 fly/trap/day) was noted in study areas. The present study indicated that trypanosomosis is the major cattle production constraint in the areas. Hence, applicable management techniques of the disease and its vector should be implemented and further investigation involving molecular technique should be conducted in different seasons.

Knowledge and perception of community about causes of cattle abortion and case-control study of brucellosis as cause of abortion in Jimma zone, Ethiopia.

BACKGROUND: Abortion, causes by several infectious and non-infectious factors is one of the most critical health problems of cattle in Ethiopia. Thus, this study aimed to assess knowledge and perception of the community about potential causes of abortion and case-control study of brucellosis as a cause of abortion in cattle of study districts. METHODS: A cross-sectional study was conducted between October 2016 and October 2017 in selected districts of the Jimma zone using a questionnaire survey. Based on the questionnaire survey, we also carried out a case-control study (141 cases and 282 controls) to confirm whether the most frequently mentioned disease (brucellosis) is associated with abortion. The blood samples were collected from both cases and controls cattle groups and then serum was separated. The presence of antibody against Brucella organism in serum was first tested by Rose Bengal Plate Test (RBPT) and all RBPT positive samples were confirmed using the Complement Fixation Test (CFT). RESULTS: From a total of 180 randomly selected respondents, the majority (59.4%) of them attributed abortion to infectious diseases. Based on the questionnaire survey, brucellosis, leptospirosis and listeriosis were identified as the major infectious causes of abortion in the areas. Physical injuries, shortage of feed and toxic substances were observed as less important non-infection causes of abortion in the study areas. This study also identified improper disposal of aborted materials and birth products, use of communal bulls, sharing communal grazing areas and water sources which favour disease transmission. Exposure to Brucella organism was higher among cases (6.4%) than controls (2.8%) with a statistically significant difference (p = 0.042). CONCLUSION: Brucellosis is the most important infectious cause of cattle abortion in this study. Thus, important to conduct appropriate control methods and increasing public awareness of the zoonotic transmission of brucellosis are suggested. This finding also recommended the need for further study to isolate and characterise brucellosis as a cause of abortion in cattle.

Epidemiological investigation of brucellosis in breeding female cattle under the traditional production system of Jimma zone in Ethiopia.

Bovine brucellosis is a serious zoonotic infectious disease with widespread occurrence in developing countries like Ethiopia. A cross-sectional study was conducted from November 2017 to November 2018 to investigate the prevalence and associated risk factors of brucellosis in breeding female cattle under the traditional production system of the Jimma zone in Ethiopia. Blood samples were collected from a total of 423 breeding female cattle to test the presence of Brucella antibody. The presence of antibody against Brucella spp. was serially tested first by the Rose Bengal Plate test, then positive sera were confirmed using the complement fixation test. Potential risk factors for Brucella seropositivity were analyzed using multivariable logistic regression analysis. An overall 11.6% (95%CI: 6.25-16.94) and 4.3% (95%CI: 2.15-5.89) seroprevalence of brucellosis was recorded at herd and animal level, respectively in study areas. We identified age (OR=9.6, 95% CI: 2.08-44.07), breed (OR=4.5, 95%CI: 1.54-12.99), herd size (OR=10.4, 95%CI: 1.27-85.04) and species composition (OR=4.4, 95%CI: 1.31-14.89) as risk factors for Brucella infection. Herd level analysis of risk factor shows that herds kept with sheep and/goats was at higher risk (OR=3.7, 95% CI: 1.25-11.17) of acquired Brucella infection. This result showed that brucellosis was a widely spread disease in the study areas. Thus, important to carry out appropriate control methods and creating awareness on public zoonotic transmissions of brucellosis are recommended. Moreover, further investigation should be carried out to isolate and characterized brucellosis as a cause of reproduction problems in the study areas.