[IS A NONBACTERIAL PROSTATITIS NONBACTERIAL?].

The study involved 287 patients with chronic recurrent urethroprostatitis. In 83 (28.9%) of them the bacterial microflora in prostatic secretions was detected. The remaining 204 patients with nonbacterial prostatitis underwent in-depth laboratory testing. Cytological and immunological methods, electron microscopy, PCR, McCoy cell culture and developed by the authors the pre-culture pathogen accumulation technique were used to investigate urethral and prostatic smears. As a result C. trachomatis was detected in 84.8% of patients, T. vaginalis--75.5%, and viruses of the Herpesviridae family--in 68.6%. Co-infection was diagnosed in 82.4% of patients, no infection was found in 6.9% of patients. The highest diagnostic sensitivity and specificity were observed in the pre-culture pathogen accumulation technique. According to electron microscopy, T. vaginalis was presented in several morphological shapes: flagellated, spherical and amoeboid. Thus, in 93.1% of patients who had previously been diagnosed with nonbacterial prostatitis by standard bacterial culture, the in-depth microbiological examination identified atypical infections (chlamydia, herpes virus, trichomonas), revealing that the rate of real nonbacterial (non-infectious) prostatitis did not exceed 6.9%.

[Immunogenic properties and molecular-genetic features of native and inactivated Chlamydia trachomatis MT-2A serovariant D strain].

UNLABELLED: AIM. Study of antibody genesis after introduction of native and inactivated Chlamydia trachomatis MT-2A strain into Balb/c mice and analysis of molecular-genetic features of omp A gene that is responsible for its immunogenicity. MATERIALS AND METHODS: Chlamydia infection was modeled in Balb/c mice by using human isolated C. trachomatis serovariant D. RESULTS: After the introduction of this strain, 5 days after the infection of mice A, M and G class antibodies including G class antibodies against chlamydia heat shock protein are induced. Studies of molecular-genetic features of the isolated C. trachomatis MT-2A strain showed presence of 2 nucleotide mutations in ompAgene. A synonymous substitution of cytosine to thymine in position 574 (CD) that does not result in amino acid substitution in the protein was registered. A non-synonymous substitution of thymine to guanine was present in position 843 (VDIII), that is most probably one of the main reasons of the registered higher immunogenicity of C. trachomatis MT-2A serovariant D strain. CONCLUSION: Further studies of C. trachomatis MT-2A serovariant D strain are promising, but do not exclude search of other immunogenic strains for the generation of agents for specific prophylaxis and immunotherapy of chlamydiosis.

[Biological and immunogenic characteristics of Chlamydia trachomatis strain MT-2A (serovar D) and its use for development of experimental inactivated vaccine].

Biological characteristics of C. trachomatis author's strain MT-2A (serovar D) is presented. Stages of development on its basis the experimental formalin-inactivated vaccine against Chlamydia were described. Humoral and cellular immune response to the vaccine administered on 3-dose immunization schedule in conjunction with polyoxidonium as adjuvant was studied. Significant immunological efficacy of the vaccine was shown. T- and B-cell immune responses were characterized. Titer of IgG antibodies against Chlamydia in blood serum after 3rd dose of the vaccine was 10,880+/-1,817.76. Assessment of T-cell response showed that reaction of delayed hypersensitivity with formation of granuloma presented in 60% of animals. Proportion of immunoblasts in reaction of blast-transformation was 29.3+/-2.8%. Perspectives of further studies of the developed corpuscular vaccine against Chlamydia are discussed.