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Graphene-based nanomaterials (graphene, graphene oxide, reduced graphene oxide, graphene quantum dots, graphene-based nanocomposites, etc.) are emerging as an extremely important class of nanomaterials primarily because of their unique and advantageous physical, chemical, biological, and optoelectronic aspects. These features have resulted in uses across diverse areas of scientific research. Among all other applications, they are found to be particularly useful in designing highly sensitive biosensors. Numerous studies have established their efficacy in sensing pathogens and other biomolecules allowing for the rapid diagnosis of various diseases. Considering the growing importance and popularity of graphene-based materials for biosensing applications, this review aims to provide the readers with a summary of the recent progress in the concerned domain and highlights the challenges associated with the synthesis and application of these multifunctional materials.
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Carbon dots (CDs) are carbon nano materials (CNMs) that find use across several biological applications because of their water solubility, biocompatible nature, eco-friendliness, and ease of synthesis. Additionally, their physiochemical properties can be chemically tuned for further optimization towards specific applications. Here, we investigate the efficacy of C70-derived Graphene Acid Quantum Dots (GAQDs) in mitigating the transformation of soluble, monomeric Hen Egg-White Lysozyme (HEWL) to mature fibrils during its amyloidogenic trajectory. Our findings reveal that GAQDs exhibit dose-dependent inhibition of HEWL fibril formation (up to 70 % at 5 mg/mL) without affecting mitochondrial membrane potential or inducing apoptosis at the same density. Furthermore, GAQDs scavenged reactive oxygen species (ROS); achieving a 50 % reduction in ROS levels at a mere 100 µg/mL when exposed to a standard free radical generator. GAQDs were not only found to be biocompatible with a human neuroblastoma-derived SHSY-5Y cell line but also rescued the cells from rotenone-induced apoptosis. The GAQD-tolerance of SHSY-5Y cells coupled with their ability to restitute cells from rotenone-dependent apoptosis, when taken in conjunction with the biocompatibility data, indicate that GAQDs possess neuroprotective potential. The data position this class of CNMs as promising candidates for resolving aberrant cellular outputs that associate with the advent and progress of multifactorial neurodegenerative disorders including Parkinson's (PD) and Alzheimer's diseases (AD) wherein environmental causes are implicated (95 % etiology). The data suggest that GAQDs are a multifunctional carbon-based sustainable nano-platform at the intersection of nanotechnology and neuroprotection for advancing green chemistry-derived, sustainable healthcare solutions.
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Apoptose , Grafite , Muramidase , Pontos Quânticos , Espécies Reativas de Oxigênio , Pontos Quânticos/química , Humanos , Grafite/química , Grafite/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Muramidase/química , Muramidase/metabolismo , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Doenças Neurodegenerativas/tratamento farmacológico , Doenças Neurodegenerativas/metabolismo , Animais , Tamanho da Partícula , Fármacos Neuroprotetores/farmacologia , Fármacos Neuroprotetores/química , Carbono/química , Propriedades de Superfície , Potencial da Membrana Mitocondrial/efeitos dos fármacosRESUMO
Nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) are persistent pollutants that have been introduced into the environment as a result of human activities. They are produced when PAHs undergo oxidation and are highly resistant to degradation, resulting in prolonged exposure and significant health risks for wildlife and humans. Nitro-PAHs' potential to induce cancer and mutations has raised concerns about their harmful effects. Furthermore, their ability to accumulate in the food chain seriously threatens the ecosystem and human health. Moreover, nitro-PAHs can disrupt the normal functioning of the endocrine system, leading to reproductive and developmental problems in humans and other organisms. Reducing nitro-PAHs in the environment through source management, physical removal, and chemical treatment is essential to mitigate the associated environmental and human health risks. Recent studies have focused on improving nitro-PAHs' phytoremediation by incorporating microorganisms and biostimulants. Microbes can break down nitro-PAHs into less harmful substances, while biostimulants can enhance plant growth and metabolic activity. By combining these elements, the effectiveness of phytoremediation for nitro-PAHs can be increased. This study aimed to investigate the impact of introducing microbial and biostimulant agents on the phytoremediation process for nitro-PAHs and identify potential solutions for addressing the environmental risks associated with these pollutants.
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Biodegradação Ambiental , Recuperação e Remediação Ambiental , Hidrocarbonetos Policíclicos Aromáticos , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Recuperação e Remediação Ambiental/métodos , Humanos , Ecossistema , Nitrocompostos/toxicidadeRESUMO
Disorders of the central nervous system (CNS) which include a wide range of neurodegenerative and neurological conditions have become a serious global issue. The presence of CNS barriers poses a significant challenge to the progress of designing effective therapeutic delivery systems, limiting the effectiveness of drugs, genes, and other therapeutic agents. Natural nanocarriers present in biological systems have inspired researchers to design unique delivery systems through biomimicry. As natural resource derived delivery systems are more biocompatible, current research has been focused on the development of delivery systems inspired by bacteria, viruses, fungi, and mammalian cells. Despite their structural potential and extensive physiological function, making them an excellent choice for biomaterial engineering, the delivery of nucleic acids remains challenging due to their instability in biological systems. Similarly, the efficient delivery of genetic material within the tissues of interest remains a hurdle due to a lack of selectivity and targeting ability. Considering that gene therapies are the holy grail for intervention in diseases, including neurodegenerative disorders such as Alzheimer's disease, Parkinson's Disease, and Huntington's disease, this review centers around recent advances in bioinspired approaches to gene delivery for the prevention of CNS disorders.
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Materiais Biocompatíveis , Técnicas de Transferência de Genes , Humanos , Materiais Biocompatíveis/química , Animais , Sistema Nervoso Central/metabolismo , Doenças Neurodegenerativas/terapia , Doenças Neurodegenerativas/tratamento farmacológico , Doenças Neurodegenerativas/metabolismo , Terapia Genética , Doenças do Sistema Nervoso Central/terapia , Doenças do Sistema Nervoso Central/tratamento farmacológico , Tamanho da Partícula , Teste de MateriaisRESUMO
Sediment contamination jeopardizes wetlands by harming aquatic organisms, disrupting food webs, and reducing biodiversity. Carcinogenic substances like heavy metals bioaccumulate in sediments and expose consumers to a greater risk of cancer. This study reports Pb, Cr, Cu, and Zn levels in sediments from eight wetlands in India. The Pb (51.25 ± 4.46 µg/g) and Cr (266 ± 6.95 µg/g) concentrations were highest in Hirakud, Cu (34.27 ± 2.2 µg/g) in Bhadrak, and Zn (55.45 ± 2.93 µg/g) in Koraput. The mean Pb, Cr, and Cu values in sediments exceeded the toxicity reference value. The contamination factor for Cr was the highest of the four metals studied at Hirakud (CF = 7.60) and Talcher (CF = 6.97). Furthermore, high and moderate positive correlations were observed between Cu and Zn (r = 0.77) and Pb and Cr (r = 0.36), respectively, across all sites. Cancer patients were found to be more concentrated in areas with higher concentrations of Pb and Cr, which are more carcinogenic. The link between heavy metals in wetland sediments and human cancer could be used to make policies that limit people's exposure to heavy metals and protect their health.
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Metais Pesados , Neoplasias , Poluentes Químicos da Água , Humanos , Áreas Alagadas , Carcinógenos/toxicidade , Chumbo , Sedimentos Geológicos , Monitoramento Ambiental , Metais Pesados/toxicidade , Metais Pesados/análise , Neoplasias/induzido quimicamente , Medição de Risco , Poluentes Químicos da Água/toxicidade , Poluentes Químicos da Água/análise , ChinaRESUMO
Environmental agents such as pesticides, weedicides and herbicides (collectively referred to as pesticides) are associated with the onset and pathogenesis of neurodegenerative disorders such as Parkinson's (PD) and Alzheimer's (AD) diseases. The development of blood-brain barrier (BBB)-penetrating therapeutic candidates to both prevent and treat the aforementioned xenotoxicant-induced neurodegenerative disorders remains an unmet need. Here, we examine whether caffeic-acid based Carbon Quantum Dots (CACQDs) can intervene in pesticide-associated onset and progress of the PD phenotype. Pulse-chase fluorescence analyses revealed that CACQDs intervene in the soluble-to-toxic transformation of the amyloid-forming protein model Hen Egg White Lysozyme (HEWL). The sp2-rich CACQDs also scavenged free radicals, a milestone along the PD trajectory. In-vitro, CACQDs introduced into a human neuroblastoma-derived cell line (SH-SY5Y) demonstrated negligible cytotoxicity up to 5 mg/mL and protected the cell line against oxidative stress-induced neuronal injury induced by the pesticide and potent neurotoxin, paraquat. Our findings suggest that the potentially BBB-penetrating CACQDs derived from caffeic acid hold promise for mitigating neurodegenerative disorders associated with environmental pesticides and xenobiotic neurotoxicants. Importantly, CACQDs sourced from coffee, coupled with their facile synthesis, represent a sustainable, green chemistry platform for generating interventional candidates in neurodegeneration.
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Metals are micropollutants that cannot be degraded by microorganisms and are infiltrated into various environmental media, including both freshwater and marine water. Metals from polluted water are absorbed by many aquatic species, especially fish. Fish is a staple food in the diets of many regions in the world; hence, both the type and concentration of metals accumulated and transferred from contaminated water sources to fish must be determined and assessed. In this study, the heavy metal concentration was determined and assessed in fish collected from freshwater sources via published literature and Estimated Daily Intake (EDI), Target hazard quotient (THQ), and Carcinogenic Risk (CR) analyses, aiming to examine the metal pollution in freshwater fish. The fish was used as a bioindicator, and Geographic information system (GIS) was sued to map the polluted regions. The results confirmed that Pb was detected in fish sampled at 28 locations, Cr at 24 locations, Cu and Zn at 30 locations, with values Pb detected ranging from 0.0016 mg kg-1 to 44.3 mg kg-1, Cr detected ranging from 0.07 mg kg-1 to 27 mg kg-1, Cu detected ranging from 0.031 mg kg-1 to 35.54 mg kg-1, and Zn detected ranging from 0.242 mg kg-1 to 103.2 mg kg-1. The strongest positive associations were discovered between Cu-Zn (r = 0.74, p < 0.05) and Cr-Zn (r = 0.57, p < 0.05). Spatial distribution maps depicting the consumption of fish as food and its corresponding Pb and Cr intake revealed a higher incidence of both carcinogenic and non-carcinogenic health concerns attributed to Pb and Cr in the region with populations consuming the fish.
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Metais Pesados , Poluentes Químicos da Água , Animais , Carcinógenos/análise , Saúde Pública , Chumbo/análise , Monitoramento Ambiental , Metais Pesados/análise , Água Doce/análise , Peixes , Poluição da Água/análise , Água/análise , Medição de Risco , Poluentes Químicos da Água/análise , Contaminação de Alimentos/análiseRESUMO
Metal-organic frameworks (MOFs) are amongst the most attractive porous polymeric networks with appealing properties. However, their inherent fragility, powder nature, low processibility, and handling present some exceptional challenges for high-tech commercial applications. Currently, economic and environmental concerns drive the development of some bioinspired polymeric matrices containing MOFs. As an artifact, the availability of previously unattainable properties is negotiated by conjugating cellulosic materials with crystalline MOFs. Thus, multi-dimensional organic-inorganic hybrid composites are formed with high electrical, optical, mechanical, and thermal features. These MOF/cellulose hybrids, known as CelloMOFs (cellulose MOFs), have remarkable mechanical properties with tunable porosities, specific surface area and accessible active sites, making them ideal for real-world troubleshooting applications such as wastewater treatment, chemical sensing, energy storage, and so on. In this review, current state-of-the-art strategic synthesis routes for fabrication of MOF/cellulose composites with a specific focus on CelloMOFs as a potential tool for mitigation of the targeted emerging water contaminants have been done under the same umbrella, which has previously been less explored. Streamlining discussions on general properties such as raw material selection, structural analysis of cellulose, availability of surface functional groups, cellulose-metal node interactions, cellulose charging, and so on have been emphasized, as has integration with robust MOFs. A better understanding of these fundamental properties is critical because they will have a significant impact on the performance of MOF/cellulose composites in a variety of applications. Furthermore, at the end of this review, the challenges and perspectives of using CelloMOFs have been discussed in a concise manner in order to improve their practical utility rather than just concept mapping.
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Estruturas Metalorgânicas , Purificação da Água , Celulose , Polímeros , EletricidadeRESUMO
A consortium of two biosurfactant-producing bacteria (Bacillus pumilus KS2 and Bacillus cereus R2) was developed to remediate petroleum hydrocarbon-contaminated paddy soil. Soil samples from a heavily contaminated rice field near Assam's Lakwa oilfield were collected and placed in earthen pots for treatment. After each month of incubation, 50 g of soil from each earthen pot was collected, and the soil TPH (ppm) in each sample was determined. The extracted TPH samples were analysed by Gas chromatography-mass spectrometry (GC-MS) to confirm microbial degradation. The soil samples were examined for changes in pH, conductivity, total organic content (TOC), water holding capacity, and total nitrogen content in addition to TPH degradation. An increasing trend in TPH degradation was observed with each passing month. After six months of treatment, the sample with the lowest initial TPH concentration (1735 ppm) had the highest degradation (91.24%), while the soil with the highest amount of TPH (5780 ppm) had the lowest degradation (74.35%). A wide range of aliphatic hydrocarbons found in soil samples was degraded by the bacterial consortium. The soil samples contained eight different low- and high-molecular-weight PAHs. Some were fully mineralized, while others were significantly reduced. With the decrease in the TPH level in the polluted soil, a significant improvement in the soil's physicochemical qualities (such as pH, electrical conductivity, total organic content, and water-holding capacity) was observed.
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Oryza , Petróleo , Poluentes do Solo , Esgotos/microbiologia , Solo/química , Biodegradação Ambiental , Poluentes do Solo/análise , Microbiologia do Solo , Hidrocarbonetos , Bactérias/metabolismo , Petróleo/análise , Petróleo/metabolismoRESUMO
Global water scarcity is exacerbated by climate change, population growth, and water pollution. Over half of the world's population will be affected by water shortages for at least a month annually by 2050 due toa lack of clean water sources. Even though recycling wastewater helps meet the growing demand, new pollutants, including pharmaceuticals and personal care products (PPCPs), pose a health threat since conventional methods cannot remove them and their environmental monitoring regulations are yet in place. Therefore, the current review aims to investigate and propose eco-friendly technologies for removing PPCPs from wastewater and their implementation strategies for ecosystem safety. Findings indicated the absence of a single wastewater treatment technology that can remove all PPCPs in a single operation. Instead, biotechnological methods are one of the alternatives that can remove PPCPs from aquatic environments. In this context, community involvement and knowledge transfer are identified keys to clean water resources' long-term sustainability.
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Cosméticos , Poluentes Químicos da Água , Águas Residuárias , Ecossistema , Eliminação de Resíduos Líquidos , Poluentes Químicos da Água/análise , Monitoramento Ambiental , Água , Cosméticos/análise , Preparações FarmacêuticasRESUMO
Antibiotic Resistant Genes (ARGs) are an emerging environmental health threat due to the potential change in the human microbiome and selection for the emergence of antibiotic resistant bacteria. The rise of antibiotic resistant bacteria has caused a global health burden. The WHO (world health organization) predicts a rise in deaths due to antibiotic resistant infections. Since bacteria can acquire ARGs through horizontal transmission, it is important to assess the dissemination of antibioticresistant genes from anthropogenic sources. There are several sources of antibiotics, antibiotic resistant bacteria and genes in the environment. These include wastewater treatment plants, landfill leachate, agricultural, animal industrial sources and estuaries. The use of antibiotics is a worldwide practice that has resulted in the evolution of resistance to antibiotics. Our review provides a more comprehensive look into multiple sources of ARG's and antibiotics rather than one. Moreover, we focus on effective surveillance methods of ARGs and antibiotics and sustainable abiotic and biotic remediation strategies for removal and reduction of antibiotics and ARGs from both terrestrial and aquatic environments. Further, we consider the impact on public health as this problem cannot be addressed without a global transdisciplinary effort.
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Antibacterianos , Poluentes Químicos da Água , Animais , Antibacterianos/farmacologia , Bactérias/genética , Genes Bacterianos , Humanos , Águas Residuárias/análiseRESUMO
Emerging micro-pollutants have rapidly contaminated the agro-ecosystem, posing serious challenges to a sustainable future. The vast majority of them have infiltrated the soil and damaged agricultural fields and crops after being released from industry. These pollutants and their transformed products are also transported in vast quantities which further exacerbate the damage. Sustainable remediation techniques are warranted for such large amounts of contaminants. As aforementioned, many of them have been detected at very high concentrations in soil and water which adversely affect crop physiology by disrupting different metabolic processes. To combat this situation, nanomaterials and other organic amendments assisted phytoremediation ware considered as a viable alternative. It is a potent synergistic activity between the biological system and the supplied organic or nanomaterial material to eliminate emerging contaminants and micropollutants from crop fields. This can be effectively be applied to degraded crop fields and could potentially embody a green technology for sustainable agriculture.
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Poluentes Ambientais , Nanoestruturas , Poluentes do Solo , Biodegradação Ambiental , Ecossistema , Solo , Poluentes do Solo/análiseRESUMO
Nanotechnology has a wide range of agricultural applications, with emphasize on the development of novel nano-agrochemicals such as, nano-fertilizer and nano-pesticides. It has a significant impact on sustainable agriculture by increasing agricultural productivity, while reducing the use of inorganic fertilizers, pesticides, and herbicides. Nano-coating delivery methods for agrochemicals have improved agrochemical effectiveness, safety, and consistency. Biosynthesis of nanoparticles (NPs) has recently been recognized as an effective tool, contrary to chemically derived NPs, for plant abiotic and biotic stress control, and crop improvement. In this regard, fungi have tremendous scope and importance for producing biogenic NPs of various sizes, shapes, and characteristics. Fungi are potential candidates for synthesis of biogenic NPs due to their enhanced bioavailability, biological activity, and higher metal tolerance. However, their biomimetic properties and high capacity for dispersion in soil, water environments, and foods may have negative environmental consequences. Furthermore, their bioaccumulation raises significant concerns about the novel properties of nanomaterials potentially causing adverse biological effects, including toxicity. This review provides a concise outline of the growing role of fungal-mediated metal NPs synthesis, its potential applications in crop field, and associated issues of nano-pollution in soil and its future implications.
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Nanopartículas Metálicas , Nanopartículas , Praguicidas , Agricultura/métodos , Fertilizantes/análise , Fungos , Nanopartículas/química , Nanopartículas/toxicidade , Nanotecnologia/métodos , SoloRESUMO
Toxic and hazardous waste poses a serious threat to human health and the environment. Green remediation technologies are required to manage such waste materials, which is a demanding and difficult task. Here, effort was made to explore the role of Pseudomonas aeruginosa SR17 in alleviating naphthalene via catabolism and simultaneously producing biosurfactant. The results showed up to 89.2% naphthalene degradation at 35 °C and pH 7. The GC/MS analysis revealed the generation of naphthalene degradation intermediates. Biosurfactant production led to the reduction of surface tension of the culture medium to 34.5 mN/m. The biosurfactant was further characterized as rhamnolipids. LC-MS of the column purified biosurfactant revealed the presence of both mono and di rhamnolipid congeners. Rhamnolipid find tremendous application in medical field and as well as in detergent industry and since they are of biological origin, they can be used as favorable alternative against their chemical counterparts. The study demonstrated that catabolism of naphthalene and concurrent formation of rhamnolipid can result in a dual activity process, namely environmental cleanup and production of a valuable microbial metabolite. Additionally, the present-day application of rhamnolipids is highlighted.
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Glicolipídeos , Tensoativos , Biodegradação Ambiental , Glicolipídeos/química , Glicolipídeos/metabolismo , Humanos , Naftalenos , Tensoativos/químicaRESUMO
Plastic trash dumped into water bodies degrade over time into small fragments. These plastic fragments, which come under the category of micro-plastics (MPs), are generally 0.05-5 mm in size, and due to their small size they are frequently consumed by aquatic organisms. As a result, widespread MPs infiltration is a global concern for the aquatic environment, posing a threat to existing life forms. MPs easily bind to other toxic chemicals or metals, acting as vector for such toxic substances and introducing them into life forms. Polyethylene, polypropylene, polystyrene, and other polymers are emerging pollutants that are detrimental to all types of organisms. The main route for MPs into the aquatic ecosystems is through the flushing of urban wastewater. The current paper investigates the origin, environmental fate, and toxicity of MPs, shedding light on their sustainable remediation.
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Halogenated compounds are one of the largest groups of environmental-hazardous chemicals. The removal of the halogen atom from the substrate is possible by the catalytic activity of a type of enzyme called dehalogenase. Hydrolytic dehalogenases are suggested to be a good biodegradation catalyst for halogenated compounds with potential bioremediation applications. Therefore, the identification of possible bacterial strains that produce dehalogenase is of great importance. Soil microorganisms that are regularly exposed to halogenated pesticides are a major source of hydrolytic dehalogenase. Their proper identification may be useful in the production of high-quality dehalogenase. DNA stable isotope probing (DNA-SIP) is quite a useful technique for the identification of active microorganisms that assimilate specific carbon substrates and nutrients. Metagenomics combined with a stable isotope probe (SIP) technique could therefore be used to detect bacterial dehalogenases in pesticides exposed agricultural soil.
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Bactérias , Metagenômica , Bactérias/genética , Biodegradação Ambiental , Marcação por Isótopo/métodos , Isótopos , Metagenômica/métodosRESUMO
This review discusses the techniques available for detecting and inactivating of pathogens in municipal wastewater, landfill leachate, and solid waste. In view of the current COVID-19 pandemic, SARS-CoV-2 is being given special attention, with a thorough examination of all possible transmission pathways linked to the selected waste matrices. Despite the lack of works focused on landfill leachate, a systematic review method, based on cluster analysis, allows to analyze the available papers devoted to sewage sludge and wastewater, allowing to focalize the work on technologies able to detect and treat pathogens. In this work, great attention is also devoted to infectivity and transmission mechanisms of SARS-CoV-2. Moreover, the literature analysis shows that sewage sludge and landfill leachate seem to have a remote chance to act as a virus transmission route (pollution-to-human transmission) due to improper collection and treatment of municipal wastewater and solid waste. However due to the incertitude about virus infectivity, these possibilities cannot be excluded and need further investigation. As a conclusion, this paper shows that additional research is required not only on the coronavirus-specific disinfection, but also the regular surveillance or monitoring of viral loads in sewage sludge, wastewater, and landfill leachate. The disinfection strategies need to be optimized in terms of dosage and potential adverse impacts like antimicrobial resistance, among many other factors. Finally, the presence of SARS-CoV-2 and other pathogenic microorganisms in sewage sludge, wastewater, and landfill leachate can hamper the possibility to ensure safe water and public health in economically marginalized countries and hinder the realization of the United Nations' sustainable development goals (SDGs).
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COVID-19 , Poluentes Químicos da Água , Humanos , Pandemias , SARS-CoV-2 , Resíduos Sólidos/análise , Instalações de Eliminação de Resíduos , Águas Residuárias , Poluentes Químicos da Água/análiseRESUMO
In developing countries, the use of antibiotics has helped to reduce the mortality rate by minimizing the deaths caused by pathogenic infections, but the costs of antibiotic contamination remain a major concern. Antibiotics are released into the environment, creating a complicated environmental problem. Antibiotics are used in human, livestock and agriculture, contributing to its escalation in the environment. Environmental antibiotics pose a range of risks and have significant effects on human and animal health. Nevertheless, this is the result of the development of antibiotic-resistant and multi-drug-resistant bacteria. In the area of health care, animal husbandry and crop processing, the imprudent use of antibiotic drugs produces antibiotic-resistant bacteria. This threat is the deepest in the developing world, with an estimated 700,000 people suffering from antibiotic-resistant infections each year. The study explores how bacteria use a wide variety of antibiotic resistance mechanism and how these approaches have an impact on the environment and on our health. The paper focuses on the processes by which antibiotics degrade, the health effects of these emerging contaminants, and the tolerance of bacteria to antibiotics.
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Microbial fuel cells (MFCs) that generate bioelectricity from biodegradable waste have received considerable attention from biologists. Fungi play a significant role as both anodic and cathodic catalysts in MFCs. Saccharomyces cerevisiae is a fungus with an ability to transfer electrons through mediators such as methylene blue (MB), neutral red (NR) or even without a mediator. This unique role of fungal cells in exocellular electron transfer (EET) and their interactions with electrodes hold a lot of promise in areas such as wastewater treatment where yeast cell-based MFCs can be used. The present article highlights the physico-chemical factors affecting the performance of fungal-mediated MFCs in terms of power output and degradation of organic pollutants, along with the challenges associated with fungal MFCs. In addition, to this comparative assessment of fungal-mediated bio-electrochemical systems, their development, possible applications and potential challenges are also discussed.
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A native strain of Bacillus paramycoides isolated from the leachate of coal mine overburden rocks was investigated for its potential to produce selenium nanoparticles (SeNPs) by biogenic reduction of selenite, one of the most toxic forms of selenium. 16S rDNA sequencing was used to identify the bacterial strain (SP3). The SeNPs were characterized using spectroscopic (UV-Vis absorbance, dynamic light scattering, X-ray diffraction, and Raman), surface charge measurement (zeta potential), and ultramicroscopic (FESEM, EDX, FETEM) analyses. SP3 exhibited extremely high selenite tolerance (1000 mM) and reduced 10 mM selenite under 72 h to produce spherical monodisperse SeNPs with an average size of 149.1 ± 29 nm. FTIR analyses indicated exopolysaccharides coating the surface of SeNPs, which imparted a charge of -29.9 mV (zeta potential). The XRD and Raman spectra revealed the SeNPs to be amorphous. Furthermore, biochemical assays and microscopic studies suggest that selenite was reduced by membrane reductases. This study reports, for the first time, the reduction of selenite and biosynthesis of SeNPs by B. paramycoides, a recently discovered bacterium. The results suggest that B. paramycoides SP3 could be exploited for eco-friendly removal of selenite from contaminated sites with the concomitant biosynthesis of SeNPs.