Assessing the Impact of Nano-Graphene Oxide Addition on Surface Microhardness and Roughness of Glass Ionomer Cements: A Laboratory Study.

Background: Nanomaterials, including nano-graphene oxide (nGO), have emerged as promising modifiers for dental materials. Therefore, this study investigated the effect of incorporating nGO into conventional glass ionomer cement (CGIC) and resin-modified glass ionomer cement (RMGIC) on surface roughness and hardness. Methods: Sixty disk-shaped specimens (2 × 6 mm) were divided into six groups: CGIC, RMGIC, CGIC with 1 wt.% nGO, CGIC with 2 wt.% nGO, RMGIC with 1 wt.% nGO, and RMGIC with 2 wt.% nGO. Surface roughness (Ra) and Vickers microhardness (VHN) were measured using a surface profilometer and Vickers microhardness tester, respectively. Statistical analysis employed the Kruskal-Wallis and Mann-Whitney tests (p <0.05). Results: The microhardness of RMGICs significantly increased with 1% and 2% nGO (p=0.017, P=0.001, respectively), while CGICs showed a significant decrease in VHN with nGO incorporation (p=0.001). VHN values of all CGIC groups were significantly higher than those of all RMGIC groups (p=0.001). Mean surface roughness values for all CGICs were significantly higher than those of RMGIC groups (p=0.001). Within the RMGIC groups, mean Ra values of RMGIC + 1 wt.% nGO and RMGIC + 2 wt.% nGO groups decreased significantly compared to the RMGIC control group (p=0.001, p=0.001, respectively). Among CGIC groups, mean Ra values of 1 wt.% and 2 wt.% nGO/CGIC groups were significantly higher than the CGIC control group (p=0.016, p=0.001). Conclusion: Incorporating nGO into RMGICs increased surface microhardness while reducing surface roughness, offering potential advantages for clinical applications. Conversely, adding nGO to CGICs increased surface roughness and decreased surface hardness. These findings emphasize the potential benefits of utilizing nGO in RMGICs and their implications in clinical practice.

Effects of different concentrations of bromelain and papain enzymes on shear bond strength of composite resin to deep dentin using an etch-and-rinse adhesive system.

BACKGROUND: The dentin substrate can be modified by proteolytic agents, which may affect the bonding strength of adhesive systems to the treated dentin surface. Papain, a cysteine protease enzyme with antibacterial and anti-inflammatory properties, can be used for deproteinization of dentin. An alternative deproteinizing enzyme is bromelain. OBJECTIVES: This study aimed to evaluate the impact of deproteinization on the shear bond strength (SBS) of composite resin to deep dentin using different concentrations of bromelain and papain. MATERIAL AND METHODS: Sixty upper premolars were extracted and randomly divided into 5 groups (n = 12 per group). In all groups, the dentin surface was etched with 37% phosphoric acid. Group 1 did not receive any enzyme treatment, group 2 was treated with a 10% papain solution, group 3 was treated with a 15% papain solution, group 4 was treated with a 6% bromelain solution, and group 5 was treated with a 10% bromelain solution. After applying an etch-and-rinse adhesive system, the specimens were restored with composite resin and the SBS was measured. RESULTS: Statistically significant differences were found between groups 2 and 3 (10% papain and 15% papain, p = 0.004), groups 2 and 4 (10% papain and 6% bromelain, p = 0.017), groups 4 and 5 (6% bromelain and 10% bromelain, p = 0.021), and groups 3 and 5 (15% papain and 10% bromelain, p = 0.005). CONCLUSIONS: Deproteinization with papain and bromelain at different concentrations after acid etching did not affect the SBS of composite resin to deep dentin when using an etch-and-rinse adhesive system. However, the group deproteinized with 15% papain demonstrated a higher SBS than the group deproteinized with 10% papain, and the group deproteinized with 6% bromelain showed a higher SBS compared to the group deproteinized with 10% bromelain.

Evaluation of the microleakage of class V composite restoration after cavity treatment with Erbium, CO2 lasers, Papain, and Bromelain enzymes.

OBJECTVES: Different surface preparation and treatment methods may have dissimilar effects on the microleakage of composite resin. This study was conducted to determine the deproteinizing effect of 10% bromelain enzyme, 10% papain enzyme, CO2 , and erbium-YAG laser in regard to decrease in the microleakage of composite restorations. MATERIALS AND METHODS: Thirty teeth were selected and 60 class V cavities were prepared on the lingual and buccal sides. They were divided into six groups (n = 10): Group 1, phosphoric acid gel; Group 2, bromelain enzyme 10%; Group 3, papain enzyme 10%; Group 4, mixed papain and bromelain enzymes 10%; Group 5, CO2 laser; and Group 6, erbium-YAG laser. They were stored in basic fuchsine and dye penetration was evaluated. Kruskal-Wallis and Mann-Whitney tests were used for statistical analysis, p < 0.05 RESULTS: In both occlusal and gingival margins, comparison of microleakage between groups 1, 2, 3, 4, and 5 showed no significant differences (p = 1) and group 6 had a significant difference with other groups (p ˂ 0.001). CONCLUSIONS: Microleakage of composite resin in the dentin surface was not affected significantly using either bromelain or papain 10% enzymes or erbium laser. However, CO2 laser had a negative effect on the enamel and dentin margins and increased the microleakage. Erbium laser showed a better effect than enzymes on microleakage.

Evaluation of the Effect of Nanographene Oxide on Microleakage of Conventional and Resin-Modified Glass Ionomer.

Objectives: One of the important features of the restorative materials is the ability to seal and prevent the microleakage. Glass ionomer cement (GIC) still exhibits some microleakage despite establishing a chemical bond to the tooth. The aim of this study was to evaluate the effect of nanographene oxide (nGO) on the microleakage of conventional (CGIC) and resin-modified glass ionomer cement (RMGIC). Methods: Thirty intact extracted molars were used. Class V cavities were prepared on their buccal and lingual surfaces. The samples randomly divided into two main groups of CGIC and RMGIC; each of them was randomly subdivided into three subgroups, including the group without nGO (control), the group with 1% nGO, and the group with 2% nGO. After restoring the cavities, they were subjected to thermocycling (1,000 cycles at 5/55°C). Two percent basic fuchsin solution was used to perform the microleakage test, and then the sectioned samples were examined by a stereomicroscope 40x. Kruskal-Wallis test, Dunn's test, and Mann-Whitney U test were used to analyze the data (P < 0.05). Results: Group CGIC + 1% nGO at the gingival margin and group RMGIC + 1% nGO at both gingival and occlusal margins had significantly less microleakage than their control groups (P=0.008, P=0.002, P=0.023, respectively). Also, in these two groups, there were no significant differences between the microleakage of the occlusal and gingival margins (P=0.132, P=0.511, respectively), while in all other groups, the gingival microleakage was significantly higher than that of occlusal microleakage. Conclusions: The addition of 1% nGO significantly reduced the gingival microleakage of CGIC and the occlusal and the gingival microleakage of RMGIC, while the addition of 2% nGO did not cause a significant reduction in microleakage.

Evaluation of the effect of nano-graphene oxide on shear bond strength of conventional and resin-modified glass ionomer cement.

OBJECTIVES: Recently, nano-graphene oxide (nGO), a material with unique mechanical properties, has been introduced to improve the properties of glass ionomer cement (GIC). The purpose of this study was to investigate the effect of adding nGO on the shear bond strength (SBS) of conventional (CGIC) and resin-modified GIC (RMGIC). METHODS: Sixty intact molars were mounted and their occlusal surface was cut at a depth of 1 mm below the dentinoenamel junction. 1 wt.% and 2 wt.% of nGO (US Research Nanomaterials, Inc.) were added to CGIC and RMGIC (GC Corporation). The samples were randomly divided into six groups (n = 10), including 1: CGIC,  2: CGIC + 1% GO, 3: CGIC + 2% GO, 4: RMGIC, 5: RMGIC + 1% GO, and 6: RMGIC + 2% GO. Plastic molds were placed on the surface of the dentin pretreated with 10% polyacrylic acid (GC Corporation) and filled with prepared cement according to the manufacturer's instruction. After 24 h of storage in an incubator, the SBS test was done by the universal testing machine. Data were analyzed using two-way analysis of variance and post hoc Tukey tests (p < .05). RESULTS: In the group of CGIC, mean SBS was significantly lower than all other study groups (p < .001), and groups 5 (RMGIC + 1% GO) and 6 (RMGIC + 2% GO) showed significantly higher values compared to all other study groups (p < .001). However, the difference between groups 2 and 3, as well as the difference between groups 5 and 6, was not significant (p = .999 andp = .994, respectively). RMGI groups had significantly higher SBS than their corresponding CGIC groups. CONCLUSIONS: The addition of 1% and 2% nGO significantly increased the SBS of CGIC and RMGIC to the dentin, which can be considered as a promising point for wider clinical application of this material.

Evaluation of the Effect of Nanoparticle Graphene Oxide on Flexural Strength of Glass Ionomer Cements.

Aim: Glass ionomer (GIC) is a widely used restorative material in dentistry, but it has relatively weak mechanical properties. In this research, the effect of graphene oxide (GO) on the flexural strength of GIC was investigated. Materials and Methods: In this experimental study, 60 GIC samples in 6 groups of 10 were prepared, including Group 1: control conventional glass ionomer (CGIC), Group 2: CGIC + 1% wt of GO, Group 3: CGIC + 2% wt of GO, Group 4: control resin-modified glass ionomer (RMGI), Group 5: RMGI + 1% wt of GO, and Group 6: RMGI + 2% wt of GO. The samples were kept for 24 hours. The flexural strength of the samples was measured by using a universal testing machine. Data were analyzed by two-way ANOVA and posthoc Tukey test. (P < 0.05). Results: In the RMGI groups, the mean flexural strength value of the RMGI + 2% GO group was significantly higher than that of the RMGI control group (P=0.027). In the comparison of RMGI groups with their corresponding CGIC groups, the mean flexural strength values of all RMGI groups were significantly more than CGIC groups (P < 0.001). RMGI + 1% GO was not significantly different from control RMGI and RMGI + 2% GO (P=0.802, P=0.395, respectively). There was no significant difference between CGIC groups. Conclusion: Adding 2% by weight of GO to RMGI increases the flexural strength of RMGI, which could be of great importance in clinical practice in order to reinforce the mechanical properties of this dental material. The flexural strength of RMGI is higher than that of CGIC.

Comparison the Effect of Bromelain Enzyme, Phosphoric Acid, and Polyacrylic Acid Treatment on Microleakage of Composite and Glass Ionomer Restorations.

Statement of the Problem: Resin modified glass-ionomer cement (RMGIC) shows low microleakage values. Bromelain enzyme is a deproteinizing agent with an anti-inflammatory effect in human body.Efective cavity treatment is an important factor in reduction of microleakage. Purpose: The aim of this study was to determine the effectiveness of the deproteinizing aspect of 10% bromelain enzyme on the microleakage of RMGIC and composite restorations. Materials and Method: In this experimental study, 40 non-carious extracted human molar teeth were categorized in eight experimental groups (n=5). Standard class V cavities were prepared on the buccal and lingual surfaces of the teeth (n=10). The specimen were classified as Group 1, in which 20% polyacrylic acid (PAA) was applied on the teeth then treated with 10% bromelain enzyme; Group 2: 10% bromelain enzyme was applied; Group 3: 10% bromelain enzyme was applied and then treated with polyacrylic acid; Group 4: 20% polyacrylic acid was applied. Groups1 to 4 were restored with RMGIC (Fuji II LC, GC, Japan). Group 5: etched by 37% phosphoric acid and then treated by 10% bromelain; Group 6: 10% bromelain enzyme was applied without etching; Group 7: teeth were deproteinized with 10% bromelain enzyme and then etched with 37% phosphoric acid; and Group 8: cavities were etched with 37% phosphoric acid. In the groups 5 to 8, Adper single bond (3M, ESPE, USA) and filled with composite resin Z350 (3M, ESPE, USA). After thermocycling, the teeth were sectioned. Microleakage scores were measured using stereomicroscope (40×). Kruskal-Wallis and Mann-Whitney tests were used for data analysis. (p< 0.05). Results: Statistical analysis did not show any significant difference in occlusal and gingival margin microleakage in glass ionomer groups (1-4) (occlusal p= 0.218, gingival p= 0.192). Kruskal-Wallis revealed significant difference in occlusal and gingival margin microleakage of Groups 5 to 8 (occlusal p= 0.006 and gingival p= 0.00). Group 5 demonstrated the lowest occlusal microleakage (occlusal mean=0.00). Conclusion: Applying bromelain or polyacrylic acid did not affect the microleakage of glass ionomer filling. Due to the antinflamatory effects of bromelain, we suggest using it instead of PAA. Pretreatment of 10% bromelain enzyme after phosphoric acid significantly decreased microleakage in the occlusal and gingival margin of composite filling.

Effect of Er:YAG, Co2 lasers, papain, and bromelain enzymes dentin treatment on shear bond strength of composite resin.

OBJECTIVE: Effective bond strength of composite resin restoration leads to its durability, so evaluation of dentin surface treatment with enzymes and laser for higher bond strength is an important factor. MATERIALS AND METHODS: Sixty human molar teeth were cut at a depth of 2 mm of occlusal part and divided into six groups (n = 10). G1: etched with 37% phosphoric, G2 and G3: 10% papain or bromelain enzymes were used on the dentinal surface, G4: 10% papain and bromelain enzyme mixture were used for. Then, the specimens were washed with distilled water. In G5 and G6: Er:YAG or Co2 lasers were used on the dentin surface. An adhesive system was applied and then nanohybrid composite was placed in teflon mold and light cured. Samples were subjected to a shear bond strength (SBS) test by universal testing machines. Statistical analysis was performed, using one-way analysis of variance and Tukey HSD tests (p < .05). RESULTS: The mean SBS in G1 was significantly higher in comparison with the other groups (p < .0001). On the other hand, a comparison of mean SBS between groups 2, 3, 4, and 5 shows no significant differences (p = .221). The mean SBS in group 6 (Co2 laser) was significantly lower in comparison with the other groups (p < .0001). CONCLUSION: Results showed that SBS of composite resin to dentin was not significantly affected, using either bromelain or papain 10% enzymes or erbium laser. Co2 laser had a negative effect on dentin and decreased the SBS. Phosphoric acid has the best result.

Comparison between the effect of adding microhydroxyapatite and chitosan on surface roughness and Microhardness of resin modified and conventional glass ionomer cements.

BACKGROUND: This study aimed to compare the effect of chitosan (CH) and hydroxyapatite (HP) on the surface roughness and microhardness of a conventional glass ionomer cement (CGIC) and a resin modified glass ionomer cement (RMGIC). MATERIAL AND METHODS: 60 disk-shaped specimens (2mm x 6mm) were prepared in 6 groups; group I: CGIC, group II: RMGIC, group III: CGIC + 15% volume CH solution in liquid, group IV: CGIC +10% weight micro-HP in powder, group V: RMGIC + 15% volume CH, group VI: RMGIC + 10% weight micro-HP. After storage in deionized water at room temperature for 24 hours, the surface roughness and microhardness of the specimens were measured using a surface profilometer and Vickers microhardness (VHN) tester, respectively. Data were analyzed using two-way ANOVA, Tukey HSD test and paired t-test (P<0.05). RESULTS: The microhardness values of RMGIC and CGIC decreased significantly with the addition of micro-HP (P<0.001). None of the CH-containing GICs showed significant changes in microhardness (P = 0.552). The VHN values of CGIC were higher than RMGIC, regardless of the added substance (P<0.001). The surface roughness (Ra) values (µm) of both RMGIC and CGIC decreased significantly with the addition of CH (P = 0.004). The incorporation of micro-HP into GICs did not have a significant effect on surface roughness values (P = 0.700). The RMGIC showed less Ra values compared to the CGIC regardless of the added substance (P<0.001). The lowest and highest Ra values were observed in RMGIC + CH and CGIC + micro-HP groups, respectively. CONCLUSIONS: The addition of CH to GIC and RMGIC reduced the surface roughness and did not have an adverse effect on the microhardness. Mixing GIC and RMGIC with micro-HP resulted in microhardness reduction and did not affect the surface roughness. Key words:Glass ionomer, hydroxyapatite, chitosan, hardness, surface roughness.

Evaluation of the Effects of Bromelain and Papain Enzymes on Shear Bond Strength of Composite Resin to Enamel.

AIM: This study aimed to evaluate the effects of 6% bromelain and 10% papain enzymes on shear bond strength (SBS) of composite resin to enamel compared to conventional 37% phosphoric acid etching. MATERIALS AND METHODS: 50 human maxillary premolar teeth were randomly divided into 5 groups (G1-G5/n = 10). In G1 and G2, after etching enamel with 37% phosphoric acid for 15 seconds and washing the surface, 10% papain and 6% bromelain enzymes were used, respectively. In G3 and G4, 6% bromelain or 10% papain enzymes were applied on enamel. In G5, the enamel surface was etched with 37% phosphoric acid for 15 seconds. A two-step etch-and-rinse adhesive system (Adper Single Bond 2) was applied. A nanohybrid composite (Z350) was placed using Teflon molds. All the samples were then subjected to the SBS test using a universal testing machine. Data analysis was performed using a one-way ANOVA test followed by the Tukey test. p values less than 0.05 were considered significant. RESULTS: Comparison of the mean SBS between G1, G2, and G5 shows no significant differences (p > 0.05); however, they had higher mean SBS compared with G3 and G4 (p < 0.0001). CONCLUSIONS: The shear bond strength of composite to enamel was not affected significantly using either 6% bromelain or 10% papain enzymes after 37% phosphoric acid application. Moreover, 6% bromelain and 10% papain enzymes were not as effective as 37% phosphoric acid alone.

Effect of chitosan treatment on shear bond strength of composite to deep dentin using self-etch and total-etch adhesive systems / Efeito do tratamento com quitosana na resistência ao cisalhamento de resina compost em dentina profunda usando sistemas adesivos autocondicionantes e condicionamento total

Objective: To assess the shear bond strength (SBS) of resin composite to deep dentin, using 1 and 2.5% chitosan pretreatment as well as different adhesive systems. Material and Methods: 80 human maxillary molars were randomly divided to eight groups according to the type of adhesive system and dentin pretreatment (n = 10): I) two-step self-etch system (Clearfil SE bond); II) two-step etch-and-rinse system (Adper single bond 2); III) 2.5% chitosan + Clearfil SE bond; IV) 2.5% chitosan +etch + Adper single bond 2; V) etch + 2.5% chitosan + Adper single bond 2; VI) 1% chitosan + Clearfil SE bond; VII) 1% chitosan + etch + Adper single bond 2; VIII) etch + 1% chitosan + Adper single bond 2 (chitosan solution (w/v): 2.5 g and 1 g of chitosan (Sigma Aldrich, USA) was dissolved in 100 ml of 1% acetic acid). Plastic molds were positioned on dentin and filled with composite (Z350, 3M ESPE, USA). SBS (MPa) was tested using a universal testing machine. ANOVA tests, Tukey's test, and independent t test were used to analyze data (p ≤ 0.05). Results: The highest SBS value among self-etch groups was observed with 1% chitosan (p = 0.001). In the etch-and-rinse group, the SBS of 1% chitosan was significantly lower than the other groups. Chitosan treatment following acid etching led to higher SBS in comparison to when chitosan was applied before etching, with the significant difference in 1% concentration (p = 0.030). A predominance of mix fractures was observed in dentin. Conclusion: Improved dentin bond strength can be achieved through immediate dentin pretreatment with 1% chitosan in self-etch adhesive systems. Chitosan Pretreatment may not be advantageous for etch-and-rinse adhesive systems. (AU)

Objetivo: Avaliar a resistência ao cisalhamento (RC) da resina composta em dentina profunda, utilizando quitosana de 1 e 2,5% como pré-tratamento, e também diferentes sistemas adesivos. Materiai e métodos: 80 molares superiores humanos foram divididos aleatoriamente em oito grupos de acordo com o tipo de sistema adesivo e pré-tratamento dentinário (n = 10): I) sistema autocondicionante de dois passos (Clearfil SE bond); II) sistema convencional de dois passos (Adper Single Bond II); III) quitosana 2,5% + Clearfil SE bond; IV) quitosana 2,5% + ácido + Adper single bond; V) ácido + quitosana 2,5% + Adper single bond II; VI) quitosana 1% + Clearfil SE bond; VII) quitosana 1% + ácido + Adper single bond II; VIII) ácido + quitosana 1% + Adper single bond II (solução de quitosana (w/w): 2,5 ge 1 g de quitosana (Sigma Aldrich, EUA) foi dissolvido em 100 ml de ácido acético a 1%). Moldeiras foram posicionados na dentina e preenchidos com resina composta (Z350, 3M ESPE, EUA). O RC (MPa) foi testado em uma máquina de teste universal. Os testes ANOVA, teste de Tukey e teste t foram usados para analisar os dados (p ≤ 0,05). Resultados: O maior valor de RC entre os grupos autocondicionantes foi observado com quitosana a 1% (p = 0,001). No grupo do condicionamento total a RC da quitosana a 1% foi significativamente menor do que nos outros grupos. O tratamento com quitosana após o condicionamento ácido levou a um maior RC em comparação a quitosana aplicada antes do condicionamento, com diferença significativa na concentração de 1% (P = 0,030). Observou-se predomínio de fraturas na dentina. Conclusão: A resistência de união à dentina pode ser alcançada por meio do pré-tratamento imediato da dentina com quitosana a 1% em sistemas adesivos autocondicionantes. O pré-tratamento com quitosana pode não ser vantajoso para sistemas adesivos de condicionamento total. (AU)

Comparison of Shear Bond Strength of Three Types of Glass Ionomer Cements Containing Hydroxyapatite Nanoparticles to Deep and Superficial Dentin.

STATEMENT OF THE PROBLEM: The clinical success of glass ionomer cement (GIC) restorations depends on the strength of its bonding to dentin, yet the bond strength of nanohydroxyapatite (nHAp) added GIC to dentin needs to be investigated. PURPOSE: This study aimed to assess if the type of GIC containing nHAp and dentin depth could affect the shear bond strength (SBS). MATERIALS AND METHOD: In this experimental study, 60 freshly extracted intact third molars were randomly divided into two main groups of flat occlusal dentin with different cuts as superficial (S); just below the dentinoenamel junction (DEJ) and deep (D); 2mm below DEJ. After conditioning with 20% polyacrylic acid, each group were randomly assigned to the tested GIC (n=10) subgroups as (1) Fuji IX Extra+nHAp, (2) Fuji II LC+nHAp and (3) Zirconomer+nHAp. Plastic tubes were placed on the pre-treated surfaces and filled with one of the GIC, then stored in an incubator at 37 oC and 100% humidity for 24hr. The specimens were thermocycled at5/55 oC for 500 cycles and subjected to SBS test using a universal testing machine (1 mm/min). The data analyzed by Mann-Whitney and Kruskal-Wallis test (p< 0.05). RESULTS: The means of SBS of Fuji II LC+nHAp was significantly higher than Fuji IX+nHAp and Zirconomer+nHAp both in superficial and deep dentin (p< 0.05). The means of SBS of Fuji IX Extra+nHAp and Zirconomer+nHAp subgroups in superficial dentin were higher than deep dentin, this differences was statistically significant (p= 0.0001 and p= 0.009, respectively). CONCLUSION: It can conclude that SBS was influenced by type of GIC and depth of dentin.

Effect of Hydroxyapatite on Surface Roughness of Zirconomer, and Conventional and Resin-Modified Glass Ionomers.

Objectives: Glass ionomer cements (GICs) are among the most popular dental restorative materials, but their use is limited due to their clinical disadvantages. Many efforts have been made to improve the properties of these materials by adding various fillers. Incorporation of hydroxyapatite (HA) into the GICs is considered to improve the physical properties of restorations, and may prevent treatment failure. This study aimed to evaluate the surface roughness (Ra) of a conventional glass ionomer cement (CGIC), a resin-modified glass ionomer (RMGI) and a Zirconomer with and without micro-hydroxyapatite (µHA). Materials and Methods: This experimental study was conducted on 6 groups (n=10) including CGIC, CGIC + µHA, RMGI, RMGI + µHA, Zirconomer, and Zirconomer + µHA. A total of 60 disc-shaped samples (6 mm × 2 mm) were prepared in plastic molds and were stored in distilled water for 24 h. After polishing of the specimens, their Ra was measured by a profilometer in micrometers (µm). The data were analyzed using two and one-way ANOVA, Tukey's HSD test, and independent t-test. Results: Incorporation of µHA resulted in statistically significant differences in Ra between the study groups (P<0.05). Following the incorporation of µHA, the Ra significantly decreased in CGIC (P=0.013) and Zirconomer (P=0.003). However, addition of µHA to RMGI resulted in a significant increase in its Ra (P<0.001). Conclusion: Addition of µHA decreased the Ra of Zirconomer and CGIC, and increased the surface roughness of RMGI samples.

Effect of Papain and Bromelain Enzymes on Shear Bond Strength of Composite to Superficial Dentin in Different Adhesive Systems.

AIM: The aim of this study was to compare different dentin treatments and adhesive systems on shear bond strength (SBS) of composite to superficial dentin. MATERIALS AND METHODS: In this in vitro experimental study, 60 extracted human upper premolars were cut 0.5 mm deep under occlusal DEJ and were randomly divided into six groups (n = 10) based on the method of dentin treatment and adhesive system: (A) two steps self-etch adhesive system (Clearfil SE bond) and (B) two steps of total etch bonding system (Adper single bond 2). (C) 10% papain + Clearfil SE bond. (D) 10% papain + Adper single bond 2. (E) 6% bromelain + Clearfil SE bond. (F) 6% bromelain + Adper single bond 2. Then, a plastic mold was placed on dentin and filled with resin composite. Shear bond strength (MPa) was tested by a universal testing machine (Zwick/Roell Z020, Germany), and the data were statistically analyzed by two-way ANOVA and Tukey's test (p < 0.05) and were applied for mean comparison. RESULTS: A significant difference was detected in the SBS evaluation between the applied adhesive systems (p = 0.0007). The SBS was also significantly affected by the interaction of treatment and etching techniques (p = 0.028). The group with 10% papain as a deproteinizing agent before the self-etching adhesive system showed the highest SBS (p = 0.029). There were no significant differences considering the specimens exposed to papain before the total etch adhesive system (p = 0.13), and the specimens were exposed to bromelain enzyme before self-etch and total etch adhesive systems (p = 0.25, p = 0.84, respectively). CONCLUSION: Papain enzyme treatment with the self-etch adhesive system increased the SBS value. Additionally, the application of bromelain enzyme as dentin treatment before two adhesive systems and papain before total etch adhesive system had no effect on the SBS of composite to superficial dentin. CLINICAL SIGNIFICANCE: Application of adhesive systems and dentin treatment can influence the bond strength.

Comparison of Bromelain Enzyme, Sodium Hypochlorite, and Titanium Tetrafluoride on Shear Bond Strength of Restorative Composite to Dentin: An in vitro Study.

STATEMENT OF THE PROBLEM: Sufficient bond strength of composite restoration leads to its durability and survival; therefore, preparation of dentin surface for higher bond strength is essential. PURPOSE: Our aim is to assess the deproteinizing effect of 3% bromelain enzyme and compare it to 4% titanium tetrafluoride (TiF4) and 5% sodium hypochlorite (NaOCl) regarding the shear bond strength (SBS) of composite resin to dentin. MATERIALS AND METHOD: In this experimental study, 40 intact extracted human maxillary premolars were selected, and the occlusal surfaces of the teeth were sectioned at a depth of 2 mm from dentinoenamel junction. The teeth were divided into 4 groups (n=10). In Group 1, the teeth were etched with 37% phosphoric acid gel. In Group 2, the teeth were etched and deproteinized with 5% NaOCl. In Group 3, the teeth were etched and deproteinized with 4% TiF4. In Group 4, the teeth were etched and deproteinized with 3% bromelain enzyme. In each specimen, composites with 3 mm diameter and 2 mm height were prepared and cured. The test specimens were then stored in distilled water at room temperature for 7 days before conducting the SBS test (MPa). By universal testing machine at a crosshead speed of 1 mm/min, the results were analyzed using one-way ANOVA and Tukey test. RESULTS: One-way ANOVA test demonstrated that pretreatment of dentin with a bromelain enzyme, TiF4 solution, or NaOCl was not statistically different regarding SBS to dentin (p= 0.790). CONCLUSION: 3% bromelain enzyme can be as effective as TiF4 and NaOCl and phosphoric acid 37% in terms of the SBS of composite resin to dentin.

Evaluation of the effect of micro-hydroxyapatite incorporation on the diametral tensile strength of glass ionomer cements.

AIM: The aim of this study was to evaluate the effect of micro-hydroxyapatite (micro-HAP) incorporation on the diametral tensile strengths (DTSs) of a conventional glass ionomer cement (GIC) and a resin-modified glass ionomer cement (RMGIC). MATERIALS AND METHODS: Forty disc-shaped specimens (diameter: 6.5 mm, height: 2 mm) were prepared into four groups (n = 10) as follows: group 1, conventional GIC; Group 2, GIC + micro-HAP (15 wt %); Group 3, RMGIC; and Group 4, RMGIC + micro-HAP (15 wt %). All the specimens were stored in distilled water for 24 h at room temperature. The DTSs of the specimens were measured using a universal testing machine. Data analysis was performed using one-way ANOVA and Tukey's test (P < 0.05). RESULTS: No significant difference was found in the DTS of conventional GIC with and without micro-HAP incorporation (P > 0.05). Moreover, the DTS of RMGIC incorporated with micro-HAP was significantly lower than that of RMGIC without micro-HAP incorporation (P < 0.05). CONCLUSIONS: Micro-HAP incorporation did not affect the DTS of conventional GIC. The DTS of RMGIC was negatively influenced by the micro-HAP incorporation. Conventional GIC (with and without hydroxyapatite) exhibited a lower DTS than RMGIC (with or without hydroxyapatite).

Evaluation of the effect of home bleaching gel on microleakage of different glass ionomers reinforced with micro-hydroxyapatite.

AIM: This study aimed to evaluate the effect of home bleaching gel on microleakage of glass-ionomer cements reinforced with micro-hydroxyapatite (HAP). METHODS: Class V cavities prepared on the forty extracted third molars were restored in four groups (n = 10): Group 1, Zirconomer; Group 2, resin-modified glass ionomer (RMGI); Group 3, Zirconomer + micro-HAP (20% WT); and Group 4, RMGI + micro-HAP (20% WT). After thermocycling (1000 cycles at 5 ± 2/55 ± 2°C, a dwell time of 30 s), each group was randomly divided into two groups. The first half was kept in distilled water and the restorations of the second half were bleached with carbamide peroxide 15% (14 days, each time 6 h/day). A uniform thickness of bleaching agent (0.5-1 mm) was applied on the surfaces of the restorations, extending 1 mm beyond the margins, and the bleaching agent was exchanged every 6 h. Microleakage was evaluated using dye penetration technique that is based on the amount of dye penetration (0.5% basic fuchsine solution) from the occlusal/gingival margins up to the axial wall. RESULTS: In distilled water, no significant difference was found between the occlusal microleakage scores (P > 0.05). The lowest and highest gingival scores in distilled water were observed in Group 4 and Group 3, respectively. In bleaching environment, there was no significant difference between four groups (P > 0.05). Comparing each glass ionomer in two environments revealed statistically significant differences in gingival and occlusal microleakage of Group 4 and in occlusal microleakage of Group 1 (P < 0.05). CONCLUSIONS: Micro-HAP incorporation did not affect the microleakage of the RMGI and Zirconomer in bleaching environment and their occlusal microleakage in distilled water. The lowest and highest gingival scores in distilled water were observed for the RMGI + micro-HAP and Zirconomer + micro-HAP, respectively. Bleaching procedure negatively affects the microleakage score of RMGI + micro-HAP and occlusal microleakage of Zirconomer.

Effects of Universal and Conventional MDP Primers on the Shear Bond Strength of Zirconia Ceramic and Nanofilled Composite Resin.

STATEMENT OF THE PROBLEM: The clinical success of ceramic depends on the quality of the bond between the zirconia and resin cement. PURPOSE: In the present study, the effects of universal and conventional MDP-containing primers were evaluated on the shear bond strength of zirconia ceramic and nanofilled composite resin. MATERIALS AND METHOD: Thirty blocks of zirconia ceramic (6mm×2mm) were prepared. Then the inner surfaces were air-abraded and divided into three groups (n= 10) as follows: untreated with primer (control group, I); All- Bond Universal (group II) and Z-Prime Plus (group III). The specimens in each group were bonded with Variolink N cement to cylinders of composite resin Z350XT. After 24 hour water storage, the shear bond strength test was performed with a universal testing machine at a crosshead speed of 1mm/ min and bond strength values (MPa) were calculated and analyzed with one-way ANOVA and post hoc Tukey tests (p< 0.05). The failure mode of each specimen was evaluated under a stereomicroscope and representative specimens were analyzed by scanning electron microscopy (SEM). RESULTS: The mean shear bond strength values (MPa) were 7.58±1.62, 17.51±1.34 and 22.45±3.60 in groups I, II and III, respectively. These results indicated that the shear bond strength were significantly higher in groups II and III compared to the control group (p< 0.001). Chemical pre-treatment of zirconia with Z- Prime Plus revealed significantly higher bond strength than the All-Bond Universal adhesive (p< 0.002). All the failure modes were adhesive in the control group (I) and when using primer treatment, mixed failures occurred in 40% and 50% of specimens in groups II and III, respectively. CONCLUSION: Treatment with both primers resulted in higher bond strength values compared to the control group. The use of Z-Prime Plus treatment in combination with air-abrasion procedure resulted in the highest bond strength.

Evaluation of the effect of home bleaching agents on surface microhardness of different glass-ionomer cements containing hydroxyapatite.

BACKGROUND: Home bleaching agents may exert some negative effects on surface hardness of restorative materials such as glass-ionomer cements (GICs). Since some studies have shown that some components such as hydroxyapatite (HA), as a bioactive glass, can improve the mechanical properties of dental materials, the effect of bleaching agents on surface hardness of GICs containing hydroxyapatite is questionable. This study was designed to evaluate the effect of home bleaching agents on the surface hardness of two different commercially available GICs containing hydroxyapatite. MATERIAL AND METHODS: 80 disk-shaped specimens were made from two different GICs, including resin modified glass-ionomer and Zirconomer. Each material was divided into four groups (n=10): 1. control, 2. 20 %wt. hydroxyapatite-containing, 3. bleached and 4. bleached 20 %wt. hydroxyapatite-containing. Group 1 and 2 specimens were stored in distilled water for 2 weeks while group 3 and 4 specimens were treated with 15% carbamide peroxide in that period. Surface hardness was tested with Vickers surface hardness tester. Data were analyzed with 3-way ANOVA and mean comparison done by post hoc Tukey tests (p<0.05). RESULTS: In general RMGI had a significantly highest Vickers surface hardness value among all groups. 15% carbamide peroxide reduced surface hardness compared to control groups (RMGI and Zr) significantly. In the HA-containing GICs groups, bleaching agent did not significantly changed the surface hardness value. CONCLUSIONS: In this study we concluded that applied treatments (bleaching and adding HA) in implicit percentages reduced surface hardness of GICs. Also we suggest more studies in clinical conditions be done to verify these results. Key words:Home bleaching, Resin Modified Glass-ionomer cement, surface hardness, Zirconia-reinforced glass ionomer, hydroxyapatite.

The Effect of Titanium Tetrafluoride and Sodium Hypochlorite on the Shear Bond Strength of Methacrylate and Silorane Based Composite Resins: an In-Vitro Study.

STATEMENT OF THE PROBLEM: The bond strength of composites with different adhesive systems with dentin is an important factor in long term durability of composite restorations. The effect of titanium tetrafluoride (TiF4) as anti caries agent and sodium hypochlorite (NaOCl) as disinfectant on the shear bond of nanofilled and silorane based composite resins have not been investigated in previous studies. PURPOSE: This study was conducted to determine bond strength between dentin and two composite systems, by means of shear bond test using TiF4 and NaOCl. MATERIALS AND METHOD: Middle dentin of 60 intact extracted maxillary premolar teeth were exposed by sectioning the crowns at a depth of 2mm from central groove and parallel to the occlusal surface. Standardized smear layer was created using a 600-grit silicon carbide paper and then samples were embedded in acrylic resin blocks. Then the samples were randomly divided into 6 \groups summarized as Group I: Z350, Group II: Z350+ NaOCl, Group III: Z350+ TiF4, Group IV: P90, Group V: P90+ NaOCl, Group VI: P90+ TiF4 according to manufacturer's instruction. Then samples were subjected to shear bond strength (SBS) test using universal testing machine and data were analyzed using ANOVA and Tukey tests (p< 0.05). RESULTS: Application of 5% NaOCl caused a significant decrease in SBS of nanofilled composite resin (p= 0.004), and also silorane based composite resin (p= 0.006). Application of 4% TiF4 caused a significant increase in SBS of silorane based composite resin (p= 0.001). The effect of TiF4 on nanofilled composite was not statistically significant. CONCLUSION: Using TiF4 has a positive effect on increasing the shear bond while NaOCl has negative effect on bond strength.