RESUMO
Coxsackievirus A10 (CV-A10) infection, a prominent cause of childhood hand-foot-and-mouth disease (HFMD), frequently manifests with the intriguing phenomenon of onychomadesis, characterized by nail shedding. However, the underlying mechanism is elusive. Here, we found that CV-A10 infection in mice could suppress Wnt/ß-catenin signaling by restraining LDL receptor-related protein 6 (LRP6) phosphorylation and ß-catenin accumulation and lead to onychomadesis. Mechanistically, CV-A10 mimics Dickkopf-related protein 1 (DKK1) to interact with Kringle-containing transmembrane protein 1 (KRM1), the CV-A10 cellular receptor. We further found that Wnt agonist (GSK3ß inhibitor) CHIR99021 can restore nail stem cell differentiation and protect against nail shedding. These findings provide novel insights into the pathogenesis of CV-A10 and related viruses in onychomadesis and guide prognosis assessment and clinical treatment of the disease.
Assuntos
Infecções por Coxsackievirus , Peptídeos e Proteínas de Sinalização Intercelular , Proteína-6 Relacionada a Receptor de Lipoproteína de Baixa Densidade , Unhas , Via de Sinalização Wnt , Animais , Humanos , Camundongos , beta Catenina/metabolismo , Diferenciação Celular/efeitos dos fármacos , Infecções por Coxsackievirus/complicações , Infecções por Coxsackievirus/metabolismo , Glicogênio Sintase Quinase 3 beta/metabolismo , Doença de Mão, Pé e Boca/virologia , Doença de Mão, Pé e Boca/metabolismo , Doença de Mão, Pé e Boca/patologia , Doença de Mão, Pé e Boca/complicações , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/genética , Proteína-6 Relacionada a Receptor de Lipoproteína de Baixa Densidade/metabolismo , Proteína-6 Relacionada a Receptor de Lipoproteína de Baixa Densidade/genética , Camundongos Endogâmicos C57BL , Doenças da Unha/metabolismo , Doenças da Unha/virologia , Doenças da Unha/patologia , Unhas/metabolismo , Unhas/patologia , Fosforilação/efeitos dos fármacos , Piridinas/farmacologia , Pirimidinas , Via de Sinalização Wnt/efeitos dos fármacos , Enterovirus/metabolismo , Enterovirus/patogenicidadeRESUMO
Transforming growth factor ß (TGF-ß) and HER2 signaling collaborate to promote breast cancer progression. However, their molecular interplay is largely unclear. TGF-ß can activate mitogen-activated protein kinase (MAPK) and AKT, but the underlying mechanism is not fully understood. In this study, we report that TGF-ß enhances HER2 activation, leading to the activation of MAPK and AKT. This process depends on the TGF-ß type I receptor TßRI kinase activity. TßRI phosphorylates HER2 at Ser779, promoting Y1248 phosphorylation and HER2 activation. Mice with HER2 S779A mutation display impaired mammary morphogenesis, reduced ductal elongation, and branching. Furthermore, wild-type HER2, but not S779A mutant, promotes TGF-ß-induced epithelial-mesenchymal transition, cell migration, and lung metastasis of breast cells. Increased HER2 S779 phosphorylation is observed in human breast cancers and positively correlated with the activation of HER2, MAPK, and AKT. Our findings demonstrate the crucial role of TGF-ß-induced S779 phosphorylation in HER2 activation, mammary gland development, and the pro-oncogenic function of TGF-ß in breast cancer progression.
Assuntos
Neoplasias da Mama , Receptor ErbB-2 , Fator de Crescimento Transformador beta , Animais , Humanos , Camundongos , Neoplasias Pulmonares/secundário , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Morfogênese , Fosforilação , Proteínas Proto-Oncogênicas c-akt/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Receptor ErbB-2/química , Receptor ErbB-2/genética , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Receptor do Fator de Crescimento Transformador beta Tipo I/metabolismo , Mama/crescimento & desenvolvimentoRESUMO
The amplitude of Wnt/ß-catenin signaling is precisely controlled by the assembly of the cell surface-localized Wnt receptor signalosome and the cytosolic ß-catenin destruction complex. How these two distinct complexes are coordinately controlled remains largely unknown. Here, we demonstrated that the signalosome scaffold protein Dishevelled 2 (Dvl2) undergoes liquid-liquid phase separation (LLPS). Dvl2 LLPS is mediated by an intrinsically disordered region and facilitated by components of the signalosome, such as the receptor Fzd5. Assembly of the signalosome is initiated by rapid recruitment of Dvl2 to the membrane, followed by slow and dynamic recruitment of Axin1. Axin LLPS mediates assembly of the ß-catenin destruction complex, and Dvl2 attenuates LLPS of Axin. Compared with the destruction complex, Axin partitions into the signalosome at a lower concentration and exhibits a higher mobility. Together, our results revealed that Dvl2 LLPS is crucial for controlling the assembly of the Wnt receptor signalosome and disruption of the phase-separated ß-catenin destruction complex.
Assuntos
Complexo de Sinalização da Axina , Proteínas Desgrenhadas , Via de Sinalização Wnt , Proteína Axina/genética , Proteína Axina/metabolismo , Complexo de Sinalização da Axina/metabolismo , beta Catenina/genética , beta Catenina/metabolismo , Membrana Celular/metabolismo , Proteínas Desgrenhadas/genética , Proteínas Desgrenhadas/metabolismo , Células HEK293 , HumanosRESUMO
As a key component of Wnt signaling, Dishevelled (Dvl/Dsh) plays essential roles in development processes and adult tissue homeostasis in multicellular organisms, and its deregulation results in human development disorders and other diseases. Dvl integrates and relays complex Wnt signals by acting as a branch-point of ß-catenin-dependent canonical and ß-catenin-independent noncanonical pathways. It dynamically interacts with multiple proteins to modulate Wnt signaling, while its activity and stability are tightly controlled by other proteins. This Review summarizes the current understanding of regulation of Dvl activity, localization, and stability by post-translational modifications, aggregation, and autophagy, and the impacts on Dvl function in both Wnt signaling and biological processes.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas Wnt , Proteínas Adaptadoras de Transdução de Sinal/genética , Autofagia , Proteínas Desgrenhadas/genética , Proteínas Desgrenhadas/metabolismo , Humanos , Fosfoproteínas/genética , Processamento de Proteína Pós-Traducional , Proteínas Wnt/metabolismo , Via de Sinalização WntRESUMO
The intracellular multiprotein complex ß-catenin destruction complex plays a key role in Wnt/ß-catenin signaling. Wnt stimulation induces the assembly of the receptor-associated signalosome and the inactivation of the destruction complex, leading to ß-catenin accumulation and transcriptional activation of the target genes. The core components of the destruction complex include Axin, APC, GSK3ß, CK1α and other proteins. Recent studies demonstrated that Axin and APC undergo liquid-liquid phase separation (LLPS), which is critical for their function to regulate Wnt/ß-catenin signaling. Here, we discuss the possible roles of LLPS in Wnt/ß-catenin signaling and regulation of Axin LLPS by post-translational modifications.
Assuntos
Via de Sinalização Wnt , beta Catenina , Proteína Axina/genética , Proteína Axina/metabolismo , Núcleo Celular/metabolismo , Fosforilação , Processamento de Proteína Pós-Traducional , beta Catenina/genéticaRESUMO
In Wnt/ß-catenin signaling, the ß-catenin protein level is deliberately controlled by the assembly of the multiprotein ß-catenin destruction complex composed of Axin, adenomatous polyposis coli (APC), glycogen synthase kinase 3ß (GSK3ß), casein kinase 1α (CK1α), and others. Here we provide compelling evidence that formation of the destruction complex is driven by protein liquid-liquid phase separation (LLPS) of Axin. An intrinsically disordered region in Axin plays an important role in driving its LLPS. Phase-separated Axin provides a scaffold for recruiting GSK3ß, CK1α, and ß-catenin. APC also undergoes LLPS in vitro and enhances the size and dynamics of Axin phase droplets. The LLPS-driven assembly of the destruction complex facilitates ß-catenin phosphorylation by GSK3ß and is critical for the regulation of ß-catenin protein stability and thus Wnt/ß-catenin signaling.
Assuntos
Complexos Multiproteicos/metabolismo , Via de Sinalização Wnt , beta Catenina/metabolismo , Proteína da Polipose Adenomatosa do Colo/genética , Proteína da Polipose Adenomatosa do Colo/metabolismo , Glicogênio Sintase Quinase 3 beta/genética , Glicogênio Sintase Quinase 3 beta/metabolismo , Células HCT116 , Células HEK293 , Humanos , Complexos Multiproteicos/genética , beta Catenina/genéticaAssuntos
Quinase do Linfoma Anaplásico/metabolismo , Neoplasias/metabolismo , Proteína Smad4/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Proteínas Supressoras de Tumor/metabolismo , Quinase do Linfoma Anaplásico/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias/genética , Neoplasias/patologia , Fosforilação , Ligação Proteica , Proteína Smad4/genética , Ativação Transcricional , Fator de Crescimento Transformador beta/genética , Proteínas Supressoras de Tumor/genética , Tirosina/metabolismoRESUMO
TGFß signaling inhibits cell proliferation to block cancer initiation, yet it also enhances metastasis to promote malignancy during breast cancer development. The mechanisms underlying these differential effects are still unclear. Here, we report that HER2/EGFR signaling switches TGFß function in breast cancer cells from antiproliferation to cancer promotion. Inhibition of HER2/EGFR activity attenuated TGFß-induced epithelial-mesenchymal transition and migration but enhanced the antiproliferative activity of TGFß. Activation of HER2/EGFR induced phosphorylation of Smad3 at Ser208 of the linker region through AKT, which promoted the nuclear accumulation of Smad3 and subsequent expression of the genes related to EMT and cell migration. In contrast, HER2/EGFR signaling had no effects on the nuclear localization of Smad2. Knockdown of Smad3, but not Smad2, blocked TGFß-induced breast cancer cell migration. We observed a positive correlation between the nuclear localization of Smad3 and HER2 activation in advanced human breast cancers. Our results demonstrate a key role for HER2/EGFR in differential regulation of Smad3 activity to shift TGFß function from antitumorigenic to protumorigenic during breast cancer development.Significance: TGFß signaling can shift from inhibiting to promoting breast cancer development via HER2/EGFR AKT-mediated phosphorylation of Smad3 at S208, enhancing its nuclear accumulation and upregulation of EMT-related genes.Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/78/21/6073/F1.large.jpg Cancer Res; 78(21); 6073-85. ©2018 AACR.
Assuntos
Neoplasias da Mama/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Receptor ErbB-2/metabolismo , Transdução de Sinais , Fator de Crescimento Transformador beta1/metabolismo , Adulto , Idoso , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Transição Epitelial-Mesenquimal/genética , Receptores ErbB/metabolismo , Feminino , Regulação Neoplásica da Expressão Gênica , Células HEK293 , Humanos , Neoplasias Pulmonares/metabolismo , Pessoa de Meia-Idade , Metástase Neoplásica , Fosforilação , Regulação para CimaRESUMO
Transforming growth factor-ß (TGF-ß) signaling regulates cell proliferation, differentiation, migration and death, and plays a critical role in embryogenesis and tissue homeostasis. Its deregulation results in various diseases including tumor formation. Receptor tyrosine kinases (RTKs), such as epidermal growth factor receptor (EGFR), fibroblast growth factor receptor (FGFR), vascular endothelial growth factor receptor (VEGFR) and platelet-derived growth factor receptor (PDGFR), also play key roles in the development and progression of many types of tumors. It has been realized that TGF-ß signaling and RTK pathways interact with each other and their interplay is important for cancer development. They are mutually regulated and cooperatively modulate cell survival and migration, epithelial-mesenchymal transition, and tumor microenvironment to accelerate tumorigenesis and tumor metastasis. RTKs can modulate Smad-dependent transcription or cooperate with TGF-ß to potentiate its oncogenic activity, while TGF-ß signaling can in turn control RTK signaling by regulating their activities or expression. This review summarizes current understandings of the interplay between TGF-ß signaling and RTKs and its influence on tumor development.