Continuous Manipulation and Characterization of Colloidal Beads and Liposomes via Diffusiophoresis in Single- and Double-Junction Microchannels.

We reveal a physical mechanism that enables the preconcentration, sorting, and characterization of charged polystyrene nanobeads and liposomes dispersed in a continuous flow within a straight micron-sized channel. Initially, a single Ψ-junction microfluidic chip is used to generate a steady-state salt concentration gradient in the direction perpendicular to the flow. As a result, fluorescent nanobeads dispersed in the electrolyte solutions accumulate into symmetric regions of the channel, appearing as two distinct symmetric stripes when the channel is observed from the top via epi-fluorescence microscopy. Depending on the electrolyte flow configuration and, thus, the direction of the salt concentration gradient field, the fluorescent stripes get closer to or apart from each other as the distance from the inlet increases. Our numerical and experimental analysis shows that although nanoparticle diffusiophoresis and hydrodynamic effects are involved in the accumulation process, diffusio-osmosis along the top and bottom channel walls plays a crucial role in the observed particles dynamics. In addition, we developed a proof-of-concept double Ψ-junction microfluidic device that exploits this accumulation mechanism for the size-based separation and size detection of nanobeads as well as for the measurement of zeta potential and charged lipid composition of liposomes under continuous flow settings. This device is also used to investigate the effect of fluid-like or gel-like states of the lipid membranes on the liposome diffusiophoretic response. The proposed strategy for solute-driven manipulation and characterization of colloids has great potential for microfluidic bioanalytical testing applications, including bioparticle preconcentration, sorting, sensing, and analysis.

Robust fabrication of ultra-soft tunable PDMS microcapsules as a biomimetic model for red blood cells.

Microcapsules with liquid cores encapsulated by thin membranes have many applications in science, medicine and industry. In this paper, we design a suspension of microcapsules which can flow and deform like red blood cells (RBCs), as a valuable tool to investigate microhaemodynamics. A reconfigurable and easy-to-assemble 3D nested glass capillary device is used to robustly fabricate water-oil-water double emulsions which are then converted into spherical microcapsules with hyperelastic membranes by cross-linking the polydimethylsiloxane (PDMS) layer coating the droplets. The resulting capsules are monodisperse to within 1% and can be made in a wide range of size and membrane thickness. We use osmosis to deflate by 36% initially spherical capsules of diameter 350 µm and a membrane thickness of 4% of their radius. Hence, we can match the reduced volume of RBCs but not their biconcave shape, since our capsules adopt a buckled shape. We compare the propagation of initially spherical and deflated capsules under constant volumetric flow in cylindrical capillaries of different confinements. We find that only deflated capsules deform broadly like RBCs over a similar range of capillary numbers Ca - the ratio of viscous to elastic forces. Similarly to the RBCs, the microcapsules transition from a symmetric 'parachute' to an asymmetric 'slipper'-like shape as Ca increases within the physiological range, demonstrating intriguing confinement-dependent dynamics. In addition to biomimetic RBC properties, high-throughput fabrication of tunable ultra-soft microcapsules could be further functionalized and find applications in other areas of science and engineering.

Red blood cell dynamics in extravascular biological tissues modelled as canonical disordered porous media.

The dynamics of blood flow in the smallest vessels and passages of the human body, where the cellular character of blood becomes prominent, plays a dominant role in the transport and exchange of solutes. Recent studies have revealed that the microhaemodynamics of a vascular network is underpinned by its interconnected structure, and certain structural alterations such as capillary dilation and blockage can substantially change blood flow patterns. However, for extravascular media with disordered microstructure (e.g. the porous intervillous space in the placenta), it remains unclear how the medium's structure affects the haemodynamics. Here, we simulate cellular blood flow in simple models of canonical porous media representative of extravascular biological tissue, with corroborative microfluidic experiments performed for validation purposes. For the media considered here, we observe three main effects: first, the relative apparent viscosity of blood increases with the structural disorder of the medium; second, the presence of red blood cells (RBCs) dynamically alters the flow distribution in the medium; third, symmetry breaking introduced by moderate structural disorder can promote more homogeneous distribution of RBCs. Our findings contribute to a better understanding of the cell-scale haemodynamics that mediates the relationship linking the function of certain biological tissues to their microstructure.

Two-dimensional micromodels for studying the convective dissolution of carbon dioxide in 2D water-saturated porous media.

Convective dissolution is a perennial trapping mechanism of carbon dioxide in geological formations saturated with an aqueous phase. This process, which couples dissolution of supercritical CO2, convection of the liquid containing the dissolved CO2, and mixing of the latter within the liquid, has so far not been studied in two-dimensional porous media. In order to do so, two-dimensional (2D) porous micromodels (patterned Hele-Shaw cells) have been fabricated from UV-curable NOA63 glue. NOA63 is used instead of PDMS, which is permeable to CO2 and does not allow for a controlled no flux boundary condition at the walls. The novel fabrication protocol proposed here, based on the bonding of a patterned photo-lithographed NOA63 layer on a flat NOA63 base, shows good reproducibility regardless of the patterns' typical size, and allows for easy filling of the cell despite the small value of the gap. A pressure chamber allows pressurizing the CO2 and outside of the flow cell up to 10 bars. Experiments were performed in 11 different porous media geometries. As expected, a gravitational fingering instability is observed upon injection of gaseous carbon dioxide in the cell, resulting in the downwards migration of dissolved CO2 plumes through the 2D porous structure. The initial wavelength of the fingers is larger in the presence of a hexagonal lattice of pillars. This effect can be correctly predicted from the theory for the gravitational instability in a Hele-Shaw cell devoid of pillars, provided that the permeability of the hexagonal porous medium is considered in the theory instead of that of the Hele-Shaw cell. Fluctuations around the theoretical prediction observed in the data are mostly attributed to a hitherto unknown weak locking of the wavelength on the distance between closest pillars.

Enhanced Accumulation of Colloidal Particles in Microgrooved Channels via Diffusiophoresis and Steady-State Electrolyte Flows.

The delivery of colloidal particles in dead-end microstructures is very challenging, since these geometries do not allow net flows of particle-laden fluids; meanwhile, diffusive transport is slow and inefficient. Recently, we introduced a novel particle manipulation strategy, based on diffusiophoresis, whereby the salt concentration gradient between parallel electrolyte streams in a microgrooved channel induces the rapid (i.e., within minutes) and reversible accumulation, retention, and removal of colloidal particles in the microgrooves. In this study, we investigated the effects of salt contrast and groove depth on the accumulation process in silicon microgrooves and determined the experimental conditions that lead to a particle concentration peak of more than four times the concentration in the channel bulk. Also, we achieved an average particle concentration in the grooves of more than twice the concentration in the flowing streams and almost 2 orders of magnitude larger than the average concentration in the grooves in the absence of a salt concentration gradient. Analytical sufficient and necessary conditions for particle accumulation are also derived. Finally, we successfully tested the accumulation process in polydimethylsiloxane microgrooved channels, as they are less expensive to fabricate than silicon microgrooved substrates. The controlled and enhanced accumulation of colloidal particles in dead-end structures by solute concentration gradients has potential applications in soft matter and living systems, such as drug delivery, synthetic biology, and on-chip diagnostics.

Fungal pretreatment facilitates the rapid and valuable composting of waste cardboard.

This study investigates the waste cardboard (WCB) fungal pretreatment (Oligoporus placenta and Tremetes hirsuta) under monoculture and mixed culture and then composting for 35 d after mixing with cow dung in different ratios. Fungal pretreatment caused significant reduction in cellulose (28.3-35.8%), hemicellulose (61.4-68.4%), lignin (67.5-69.3%) content in WCB. Pretreated WCB showed better rates of decrement in total organic carbon (26.02-47.92%), carbon-to-nitrogen ratio (19.4-23.5), and lignocellulose contents, as well as incensement in total nitrogen (40.48-63.31%), total potassium (51.92-73.91%), germination index (88.5-102.0%), and elemental (Cu, Fe, Zn, Cr, and Mn) levels. Dehydrogenases (142-210 µg g-1h-1), and ß-galactosidase (210-256 µg g-1h-1) activities indicates high microbial-mediated mineralization in setups. Results suggested that WCB could be used as a valuable substrate for valuable-added compost preparation after pretreating with a consortium of white-rot fungi.

Reversible Trapping of Colloids in Microgrooved Channels via Diffusiophoresis under Steady-State Solute Gradients.

The controlled transport of colloids in dead-end structures is a key capability that can enable a wide range of applications, such as biochemical analysis, drug delivery, and underground oil recovery. This Letter presents a new trapping mechanism that allows the fast (i.e., within a few minutes) and reversible accumulation of submicron particles within dead-end microgrooves by means of parallel streams with different salinity level. For the first time, particle focusing in dead-end structures is achieved under steady-state gradients. Confocal microscopy analysis and numerical investigations show that the particles are trapped at a flow recirculation region within the grooves due to a combination of diffusiophoresis transport and hydrodynamic effects. Counterintuitively, the particle velocity at the focusing point is not vanishing and, hence, the particles are continuously transported in and out of the focusing point. The accumulation process is also reversible and one can cyclically trap and release the colloids by controlling the salt concentration of the streams via a flow switching valve.

3D Arrays of Super-Hydrophobic Microtubes from Polypore Mushrooms as Naturally-Derived Systems for Oil Absorption.

Porous materials derived from natural resources, such as Luffa sponges, pomelo peel and jute fibres, have recently emerged as oil adsorbents for water purification, due to their suitability, low environmental impact, biodegradability and low cost. Here we show, for the first time, that the porosity of the fruiting body of polypore mushrooms can be used to absorb oils and organic solvents while repelling water. We engineered the surface properties of Ganoderma applanatum fungi, of which the fruiting body consists of a regular array of long capillaries embedded in a fibrous matrix, with paraffin wax, octadecyltrichlorosilane (OTS) and trichloro(1H,1H,2H,2H-perfluorooctyl)silane. Morphological and wettability analyses of the modified fungus revealed that the OTS treatment was effective in preserving the 3D porosity of the natural material, inducing super-hydrophobicity (water contact angle higher than 150°) and improving oil sorption capacity (1.8⁻3.1 g/g). The treated fungus was also inserted into fluidic networks as a filtration element, and its ability to separate water from chloroform was demonstrated.