Seasonal Variation in the Body Composition, Carcass Composition, and Offal Quality in the Wild Fallow Deer (Dama dama L.).

The goal of this study was to examine the seasonal variation in the body composition, carcass composition, and quality of edible internal organs from the fallow deer hunt-harvested in the summer (n = 9) and the winter (n = 10) seasons. The weight and proportion of the mesenteric and omental fat were greater for the animals harvested in the winter (1.12 kg and 2.75%) compared to those from the summer season (0.43 kg and 1.02%). The winter-harvested animals had more perinephric fat (0.75 kg and 1.84%) than those hunted in summer (0.26 kg and 1.84%). The gastrointestinal tract of the fallow deer hunted in summer was more filled with feed and therefore heavier (7.92 kg) compared to those from the winter season (5.16 kg). The proportion of fat was significantly greater in the carcasses obtained in winter compared to the summer season (6.55% vs. 3.79%). No seasonal variety was found in the physicochemical characteristics of the edible offal, but the content of extractable fat was significantly affected by the season. In conclusion, the effect of the season on the slaughter value of the hunt-harvested fallow deer was limited to a variation in the proportion of some of the internal organs and affected the fat deposition in the body of the examined animals. The season significantly affected the fat content in the carcass and the extractable fat content in the examined offal.

Prevalence of Babesia canis DNA in Ixodes ricinus ticks collected in forest and urban ecosystems in west-central Poland.

Babesia canis, a widely distributed European tick-borne protozoan haemoparasite, causes canine babesiosis, the most important tick-borne disease afflicting dogs worldwide. The meadow tick, Dermacentor reticulatus, is considered to be the primary vector of this parasite in central Europe. Females of the more broadly distributed and medically important castor bean tick, Ixodes ricinus, also commonly feed upon dogs, but their role in the enzootic transmission cycle of B. canis is unclear. Here, we screened 1,598 host-seeking I. ricinus ticks collected from two different ecosystems, forest stands vs. urban recreational forests, for the presence of B. canis DNA. Ticks were sampled during their two seasonal peaks of activity, spring (May/June) and late summer (September). Babesia species were identified by amplification and sequencing of a hypervariable 18S rRNA gene fragment. Babesia canis was the only piroplasm detected in 13% of 200 larvae and 8.2% of 324 nymphs in the forest ecosystems. In urban recreational areas, B. canis DNA was found in 1.5% of 460 nymphs, 3.5% of 289 females and 3.2% of 280 males. Additionally, three samples, including one female, one male, and one nymph, were co-infected with B. venatorum and one nymph with B. divergens or B. capreoli. Our findings implicate that B. canis can be transmitted transovarially and maintained transstadially within populations of I. ricinus, but the vector competence of I. ricinus for transmitting B. canis remains to be investigated.

Predatory mite instars (Acari, Mesostigmata) and decomposing tree leaves in mixed and monoculture stands growing on a spoil heap and surrounding forests.

In the past, ecological research mainly omitted the sexual and developmental variability of mite communities, and therefore could not fully reflect the actual state and function of mite communities in the ecosystems studied. The aim here was to analyze how habitat conditions (mixed vs. monoculture stands) and single-species litter of 14 tree species (in mixed stands) affect the sex and developmental stages of Mesostigmata mites living on the decomposing litter. The research was conducted in 2011-2016, at the Belchatów Lignite Mine external spoil heap (Central Poland) in mixed stands growing on the spoil heap, as well as in pine and birch monoculture stands growing on the spoil heap and an adjacent forest area. We found significant influences of habitat on females, males and juveniles. Additionally, we found that soil mean temperature had a significant effect on males and juveniles, but not on females. Moreover, despite the insignificant influence of litter species on mite communities, we found that percentage litter mass loss significantly affected female and juvenile mites. Taking into account habitat type, the percentage litter mass loss significantly affected female and male mites, but not juveniles. The mite abundance calculated per dry litter mass usually gradually increased during decomposition. Interestingly, the highest mean female, male and juvenile abundances were recorded in birch stands growing on the adjacent forest area; however, juvenile mites were also very numerous in mixed stands on spoil heap. Therefore, our results confirm that mixed stands on post-mining areas are a potentially better habitat for development of mesostigmatid communities compared to monocultures, among others by relatively higher humidity and lower temperatures.

Factors influencing the accuracy of ground-based tree-height measurements for major European tree species.

Tree height is one of the most important forest characteristics and is one of the crucial measurements taken for either practical or scientific reasons. However, the accuracy of a tree-height measurement may vary in relation to many factors. The work described here thus sought to evaluate the accuracy of ground-based tree-height measurements for major forest-forming tree species of the temperate and boreal zones. The focus was on the importance of factors affecting accuracy of the measurements in question at larger geographical scales. In line with the above research goals, data were gathered from 299 stands throughout Poland and heights of 2388 sample trees of eight species, growing in different stands and site conditions, were measured; heights were then compared with measured lengths of felled trees as a reference. In total, 10 variables to determine factors that may influence ground-based tree-height measurement accuracy were used. We merged them into 4 groups: measurements, topography, stand and biometric-related factors. Results showed that biometric and topographic factors had the greatest relative influence on the accuracy of measurements of tree height. Tree length and species, followed by the slope of the terrain, tree age, and height above sea level were the most important factors found to affect accuracy. In most of the cases studied the terrestrial tree-height measurements were underestimated when set against definitive measurements of length. This was true for all species studied except oak, for which height measurements were typically overestimated. Notwithstanding the broad geographical scope of the work, the particular device used and the team factor were only found to have a marginal influence on measurement accuracy.

The seasonal variation in the quality of venison from wild fallow deer (Dama dama) - A pilot study.

The studied material included 19 fallow deer does hunt-harvested in two seasons, summer and winter. The post-mortem body weight of analysed animals was not affected by the season (P = 0.762). The pH value measured 24 h post-mortem in the M. longissimus lumborum was significantly higher in the summer compared to the winter season. The venison obtained in the winter season characterized with higher L* (P < 0.0001) and b* (P = 0.002) and lower a* (P < .0001) compared to meat from the summer season. There was also a seasonal variation in the purge in vacuum bags (P = 0.001), water compartments and cooking loss (P < 0.0001). Considering the proximal chemical composition, the extractable fat content was higher in winter (P < 0.0001) compared to the summer season. On the basis of the research results, one can conclude about greater usability for processing and storage of venison obtained in the winter season.

Response of soil mites (Acari, Mesostigmata) to long-term Norway spruce plantation along a mountain stream.

During the nineteenth and twentieth centuries, coniferous monocultures were introduced, replacing natural broadleaved forests in Central Europe, mainly for economic benefits. In the mountains, Norway spruce [Picea abies (L.) H. Karst] was introduced in large areas previously covered with beech forests and also in natural riverside habitat corridors such as river valleys, despite its negative impact on the soil environment by e.g. organic matter accumulation, decrease of soil pH and changes in C/N ratio. We aimed to check how long-term Norway spruce plantations affect species richness and diversity of soil mites along a mountain river in former mixed and broadleaved forests. The study, based on 342 samples, was carried out in Stolowe Mountains National Park (SW Poland). Understory species biomass, soil pH and soil organic layer thickness significantly affected soil mite communities. Although coniferous forests did not differ from either broadleaved or mixed forests in mite density (number of individuals m-2) and species diversity (H'), they were characterized by low species richness and proportional abundance of Uropodina mites typical for broadleaved forests. In total, 4849 mites classified into 57 species were recorded from all forest types and no unique species were found in the sampled forests. Although the mite communities were dominated by the same common species (Veigaia nemorensis, Paragamasus runcatellus, Leptogamasus obesus and Trachytes aegrota), they still maintain the rare species of broadleaved forests and their high recovery potential may be used in forest conversion.

Natural forest remnants as refugia for bryophyte diversity in a transformed mountain river valley landscape.

Riparian forests are among the most threatened ecosystem types worldwide. Their exploitation and replacement by coniferous plantations affects species pools and contributes to loss of biodiversity. We aimed to investigate bryophyte species pools within different habitat types in a transformed mountain river valley. We especially focused on the contribution of habitat types (relative to their proportional cover) to the species pool of the whole area. The study was conducted along the Czerwona Woda river - a model stream in the Stolowe Mountains National Park (SW Poland, study area: 91.2 ha) - and an example of coniferous plantations replacing natural broadleaved forest vegetation. Our study revealed the presence of 147 bryophyte species. The most valuable habitats in terms of diversity of bryophyte assemblages were remnants of the natural vegetation - broadleaved forests and streams. These habitats, constituting <5% of the study area, hosted ca 40% of the total species pool (61 and 62 species, respectively), while the species pool of Picea abies forests (92 species) was proportional to cover of this habitat type (ca 60%). Remnants of natural vegetation were hotspots of bryophyte diversity within the heavily altered landscape, and may play a future role as sources of recolonization by forest specialists. Our study also confirmed the important role of riparian areas in maintaining bryophyte species diversity at the landscape scale. The river valley studied contributes >20-fold more to the bryophyte species pool of the whole national park than indicated by its size. Thus, river valleys require special treatment - conservation based on natural restoration, and should remain reserved from wood production, as areas providing a wide range of ecosystem services.

Human behaviors elevating the risk of exposure to Ixodes ricinus larvae and nymphs in two types of lowland coniferous forests in west-central Poland.

Ixodes ricinus L. is the commonest tick encountered by humans in Central Europe and the most important vector of tick-borne diseases (TBD) in Europe. Foresters represent a group at elevated risk of exposure to I. ricinus throughout Europe. Here, we employed an experimental design similar to one used previously to determine what behavioral factors heighten exposure to questing Ixodes pacificus nymphs in a hardwood forest in the far-western United States. Five activities were evaluated systematically in a coniferous forest and a mixed broadleaved forest in west-central Poland: (i) walking in low-cut running shoes; (ii) sitting atop logs; (iii) sitting on leaf litter; (iv) sitting against tree trunks; and (v) gathering firewood. In total, 540 I. ricinus ticks were collected (198 larvae, 327 nymphs, 15 adults) in spring and late summer. Many more ticks were collected in spring than in late summer, and in the mixed broadleaved forest versus the coniferous forest. The riskiest behavior for acquiring nymphs in both spring and late summer was gathering firewood, though sitting atop logs was nearly as perilous in late summer. In contrast, the riskiest behaviors for encountering larvae in spring and late summer were sitting against trunks and sitting on leaf litter, respectively. Pairwise comparisons revealed only two significant associations among the variables explored for the nymphs: season and human gender, and season and type of forest. For larval ticks, the most significant associations were gender and sitting behaviors and for type of forest and sitting on leaf litter or against tree trunks. The most commonly infested body region was the lower legs.

Syringophilid mites (Acari: Syringophilidae) associated with the rails (Aves: Rallidae) and a key to the species of the genus Rafapicobia Skoracki, 2011.

The fauna of quill mites of the family Syringophilidae Lavoipierre, 1953 (Acari: Prostigmata Cheyletoidea) parasitising birds of the family Rallidae Vigors (Gruiformes) is updated. A new species, Rafapicobia melzeri n. sp. (subfamily Picobiinae), is described from four host species: Rallus aquaticus Linnaeus (type-host) from Germany, Pardirallus sanguinolentus (Swainson) from Chile, Porzana porzana (Linnaeus) from France and P. parva (Scopoli) from Kirghizia. The new species is most similar to R. lepidocolaptesi Skoracki & Solarczyk, 2012 but differs in the absence of agenital plates and the length ratios of setae ag2:g1 and vi:ve:si in females and in the punctate ornament on the hysteronotal and the pygidial shields in males. A key to the species of the genus Rafapicobia is proposed. This is the first record of a representative of the subfamily Picobiinae on gruiform birds. Additionally, new rallid hosts are reported for Charadriphilus ralli Skoracki & Bochkov, 2010 (subfamily Syringophilinae): Gallinula melanops (Vieillot) from Chile, Laterallus melanophaius (Vieillot) from Paraguay, and P. parva (Scopoli) from Kirghizia.