Prostatic proliferative inflammatory atrophy: welcome to the club†.

Single-cell RNA sequencing studies in the human prostate have defined a population of epithelial cells with transcriptional similarities to club cells in the lung. However, the localization of club-like cells in the human prostate, and their relationship to prostate cancer, is poorly understood. In a new article in The Journal of Pathology, RNA in situ hybridization was used to demonstrate that club cell markers are expressed in luminal cells adjacent to inflammation in the peripheral zone of the human prostate, where prostate cancer tends to arise. These club-like cells are commonly found in proliferative inflammatory atrophy (PIA) lesions and express markers consistent with an intermediate epithelial cell-type. Future studies will be needed to understand the functional role of club-like cells in human prostate inflammation, regeneration, and disease. © 2023 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.

Farfield coherent infrasound generation using an air-propane burner.

An invaluable tool in the characterization of any receiver, propagation path, or detection system is a source with known and repeatable signal characteristics. This article presents the theoretical development and engineering design of a coherent (nonexplosive, periodic with controlled duration) infrasound source in the sub-hertz to several hertz band. Design of a sound source within this band is a difficult engineering challenge. The simple source equation, which will govern any portable human-fabricated infrasound source due to the long wavelengths, shows this fundamental difficulty. As frequency decreases, volume displacement must increase by the squared inverse factor of frequency in order to maintain an equal pressure amplitude at equal range. For this reason, the authors evaluate using the high energy density available in gas combustion to periodically displace large volumes of air within the open atmosphere. Prototype testing has verified the capability of generating continuous signals at a fundamental frequency of 0.25 Hz in the farfield-ranges in which pressure and particle velocity can be considered in-phase-where the product of the acoustic wavenumber and range is near 4.7. The generation of frequency content throughout the 0.25-4.0 Hz band with a reasonable signal-to-noise ratio was also demonstrated.

Variation in low-frequency estimates of sound levels based on different units of analysis.

The measurement and analysis of underwater sound is a complicated process because of the variable durations of contributing sources and constantly changing water column dynamics. Because the ambient sound distribution does not always follow a Gaussian structure and may be nonstationary in time, analysis over an extended period is required to accurately characterize the data. Utilizing recordings from the Indian Ocean, the temporal variation in ambient sound including transient signals was examined using multiple processing window lengths and subsampling intervals. Results illustrate the degree of uncertainty in sound levels based on different units of analysis. The average difference between sound level estimates in the 10-30 Hz band due to subsampling was 2 dB and as high as 4 dB. The difference in the full band (5-110 Hz) was as high as 6 dB. Longer averaging windows (200 vs 60 s) resulted in larger variations over different subsampling intervals. This work demonstrates how sampling protocols within a single dataset can influence results and acknowledges that comparative studies at the same location but with different sampling protocols can be substantial if signal processing parameters are not statistically accounted for to confirm interpretation of results and observed trends.

Supplemental control of lepidopterous pests on Bt transgenic sweet corn with biologically-based spray treatments.

Biologically-based spray treatments, including nucleopolyhedroviruses, neem, and spinosad, were evaluated as supplemental controls for the fall armyworm, Spodoptera frugiperda (J. E. Smith), and corn earworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), on transgenic sweet corn, Zea mays (L.) (Poales: Poaceae), expressing a Cry1Ab toxin from Bacillus thuringiensis Berliner (Bacillales: Bacillaceae) (Bt). Overall, transgenic corn supported lower densities of both pests than did nontransgenic corn. Control of the fall armyworm was improved in both whorl-stage and tassel-stage corn by the use of either a nucleopolyhedrovirus or neem, but the greatest improvement was seen with spinosad. Only spinosad consistently reduced damage to ears, which was caused by both pest species. In general, efficacy of the spray materials did not differ greatly between transgenic and nontransgenic corn.