Impact of Leg Strength on the Force Produced During a Cross-Punch in Highly Trained Amateur Boxers.

ABSTRACT: Satkunskiene, D, Bruzas, V, Mantas, M, Snieckus, A, and Kamandulis, S. Impact of leg strength on the force produced during a cross-punch in highly trained amateur boxers leg strength impact on cross-punch force. J Strength Cond Res XX(X): 000-000, 2024-The purpose of this study was to determine the impact of leg strength on force generation during the execution of a cross-punch by highly trained amateur boxers. This study involved 12 male, amateur boxers with a body mass range of 64.5-112.2 kg and a mean of 89.2 ± 17.0 kg. This study used an isokinetic dynamometer to measure the peak torque of the knee extensor and flexor muscles at 60°/s. The force plate was used to collect three-dimensional ground reaction forces (GRF), moments (GRM), and the center of pressure (COP) during a maximal cross-punch. The Kiktest-100 system was used to determine the force of the punch, while the Hykso punch tracker accelerometer measured the maximum fist velocity. This study revealed a positive correlation between knee extensor strength and GRFz, GRFy, and COPy during punch performance. In addition, the GRMz gradient and GRMz impulse during the execution phase have a significant positive effect on cross-punch force. In conclusion, the force of a straight punch in highly trained, amateur boxers is related to a greater angular velocity about the vertical axis during body rotation.

Impact of Anaerobic Exercise Integrated Into Regular Training on Experienced Judo Athletes: Running Vs. Repetitive Throws.

ABSTRACT: Kamandulis, S, Dudeniene, L, Snieckus, A, Kniubaite, A, Mickevicius, M, Lukonaitiene, I, Venckunas, T, Stasiule, L, and Stasiulis, A. Impact of anaerobic exercise integrated into regular training on experienced judo athletes: running vs. repetitive throws. J Strength Cond Res XX(X): 000-000, 2024-Anaerobic training in high-level athletes is of considerable interest to practitioners aiming to optimize performance. This study compared the impact of interval anaerobic training (IAT) sessions consisting of either high-intensity running or throwing that were performed twice a week together with regular judo training on the anaerobic and aerobic performance of experienced judo athletes. Employing a repeated-measures, counterbalancing, research design, 12 national team judo athletes (7 women and 5 men; mean age, 20.4 ± 0.95 years; mean judo training experience, 13.4 ± 1.4 years; competitive level, black belt first and second Dan) performed each IAT modality for 6 weeks, for a full training cycle of 12 weeks. Assessments of their anaerobic fitness (Cunningham and Faulkner Anaerobic Treadmill Test), sport-specific anaerobic fitness (Special Judo Fitness Test [SJFT]), and aerobic capacity (maximal incremental treadmill running test) were performed before, after 6 weeks, and after 12 weeks of training. The uphill running performance improved by 13.1% over the 12-week period (p = 0.047). Simultaneously, there was a 9.0% improvement in the SJFT index and a 6.9% increase in the number of throws (p = 0.011 and p = 0.017, respectively). Although a trend for throwing drills being more effective than interval sprint running was observed, the interaction effect lacked statistical significance (p = 0.074). Moreover, no substantial changes were noted in aerobic endurance markers. In conclusion, this study suggests that incorporating specific and nonspecific high-intensity drills into a routine training regimen may enhance anaerobic capacity among well-trained judo athletes, potentially leading to favorable competitive outcomes.

Mood, Cognitive Function, and Plasma Kynurenine Metabolites Responses Following Severe Changes in Physical Activity.

PURPOSE: To monitor changes in mood, cognitive function, brain electrical activity, and circulating kynurenine pathway metabolites in response to a three-week severe physical activity restriction, followed by three weeks of resumed activity adding resistance and high-intensity interval exercise training. METHODS: Twenty healthy participants (14 males, six females; 25.4 ± 5.2 years) underwent three weeks of limited physical activity using forearm crutches with one leg suspended (INACT) and then three weeks of resumed activity plus supervised resistance and high-intensity interval training sessions (ACT, three to six sessions per week). At baseline, after INACT, and then after ACT, venous blood was sampled for analysis of major kynurenine pathway metabolites, a short version of the International Physical Activity Questionnaire (IPAQ-SF), Hospital Anxiety and Depression Scale (HADS) and Profile of Mood States (POMS) questionnaires were completed, and cognitive tests with EEG were performed. RESULTS: During INACT, the depression score on the HADS scale tended to increase (3.5 to 6.8; p = 0.065), while it was reduced with ACT compared with after INACT (2.8; p = 0.022). On the POMS scale, depression, fatigue, and confusion increased within INACT (p < 0.05). Notably, subjects exhibited considerable variability, and those experiencing depression symptoms recorded by the HADS scale (n = 4) displayed distinct mood disturbances on POMS. All HADS and POMS scores were fully restored to baseline with ACT. Neither INACT nor ACT induced significant changes in cognition, brain electrical activity, or kynurenine pathway metabolites (p > 0.05). CONCLUSIONS: While young healthy individuals with three weeks of severely restricted physical activity do not undergo changes in circulating kynurenine pathway metabolites, cognitive performance, and brain electrical activity, their mood response is quite variable, and depression develops in some. Three weeks of resuming mobility plus exercise training reversed the mood profile.

The effects of 6-week home-based static stretching, dynamic stretching, or eccentric exercise interventions on muscle-tendon properties and functional performance in older women.

Background: Joint inflexibility is acknowledged as a significant contributor to functional limitations in the older adult, with lengthening-type exercises identified as a potential remedial approach. Nevertheless, the responses to eccentric exercise in female older adults have not been extensively studied especially in home-based environment. Here, we aimed to assess the effectiveness of home-based static stretching (ST), dynamic closed-chain stretching (DCS), or eccentric exercise (ECC) interventions on flexibility, musculotendinous architecture, and functional ability in healthy older women. Methods: We randomly assigned 51 healthy older women (age 65.9 ± 3.4 years) to one of three interventional exercise groups: DCS (N = 17), ECC (N = 17), or ST (N = 17). The training was performed 3 times a week for 6 weeks. The participants' musculotendinous stiffness, fascicle length, eccentric strength, and functional capacities were measured before the intervention, after 6 weeks of exercise, and at a 1-month follow-up. Results: The results showed that all three interventions improved hamstring flexibility and passive ankle dorsiflexion (p < 0.001), with increased biceps femoris and medial gastrocnemius fascicle length (p < 0.01). However, there was no significant change in musculotendinous stiffness. The ECC intervention produced a greater improvement in knee flexor and calf eccentric peak torque (p < 0.05), and gait speed (p = 0.024) than the other two interventions. The changes in flexibility and knee flexor strength remained for up to 4 weeks after detraining. Conclusion: In conclusion, the present study suggests that home-based ECC may be more beneficial in enhancing physical capacities in older women compared with either DCS or SS interventions.

Hamstring stiffness and injury risk factors during the handball season in female players.

Monitoring the muscle mechanical properties and functions of female athletes throughout their training season is relevant to understand the relationships between these factors and to predict noncontact injuries, which are prevalent among female athletes. The first aim of this study was to determine whether female handball players' passive stiffness of the hamstring muscles is associated with hamstring extensibility, strength of knee flexors and extensors, and lower limb stiffness. Additionally, the study monitored fluctuations in these factors over 25 weeks. The study utilized an isokinetic dynamometer to record hamstring passive stiffness, extensibility, and hamstring and quadriceps strength of 18 young handball players. Lower limb stiffness was determined from a countermovement vertical jump conducted on a force plate. The countermovement jump involved the calculation of the peak force during the eccentric phase and the mean force during the concentric phase. The results showed a positive correlation between hamstring passive stiffness and lower limb stiffness (r = 0.660, p < 0.01), knee flexion and extension strength (r = 0.592, p < 0.01 and r = 0.497, p < 0.05, respectively), and eccentric peak force (r = 0.587, p < 0.01) during jumping. The strength of knee extensors increased significantly after 6 weeks, and hamstring stiffness after 12 weeks of training. In conclusion, the increased hamstring stiffness following training did not match other factors associated with injury risk. Therefore, preventing multifactorial injury risk requires a comprehensive approach, and monitoring one factor alone is insufficient to predict noncontact injuries in female handball players.

The effects of high-velocity hamstring muscle training on injury prevention in football players.

Background: Explosive and fast body movements, sprints, jumps and quick changes of direction, which are characteristic of the football training, place considerable strain on the hamstring muscles. Due to the high occurrence of hamstring injuries, new preventive strategies are required that focus on high-velocity training. The purpose was to assess the effectiveness of high-velocity elastic-band training in reducing the occurrence of hamstring injuries in football players. Methods: Male football players from 15 teams (n = 319) playing in national competitions participated in this study. The players were involved in a 5-week exercise period in either the intervention group (INT) or the control group (CON), with a follow-up period of ∼4 months where hamstring injuries and exposure time were recorded. The INT group had two to three sessions per week of elastic-band training with low-load, high-velocity leg curls while lying prone; the CON group performed self-paced football-specific drills. Results: The incidence rate of hamstring injuries was 6.5% in the INT group (8 out of 123 players) and 9.2% in the CON group (18 out of 196 players). Although the INT group showed almost 1/3 reduction in hamstring injury incidence compared to the CON group, the difference was not statistically significant (p > 0.05). Moreover, no differences (p > 0.05, odds ratio [OR] = trivial-to-small) in distribution between the groups were found in hamstring injury characteristics (leg dominance and mechanism) except for the distribution of injuries that occurred during matches or training (p = 0.036; OR = 6.14, moderate). Conclusion: The program of high-velocity elastic-band training did not prove to be effective in preventing hamstring muscle injuries in football players despite displaying some positive indications that could be considering when creating injury prevention programs.

Recreational 3 × 3 basketball elicits higher heart rate, enjoyment, and physical activity intensities but lower blood lactate and perceived exertion compared to HIIT in active young adults.

This study aimed to assess and compare the physiological [percentage of maximal heart rate (%HRmax), blood lactate (BLa), creatine kinase (CK)], hormonal (testosterone, cortisol), psychological [rating of perceived exertion (RPE), enjoyment] and physical [percentage of moderate-to-vigorous physical activity (%MVPA) and vigorous activity (%VA)] responses of recreational 3 × 3 basketball (3 × 3BB) and high-intensity interval training (HIIT) in active young adults. Twelve apparently healthy male recreational basketball players (age: 23 ± 3 years; body mass: 82 ± 15 kg; stature: 188 ± 15 cm) completed a 3 × 3BB match and HIIT with similar duration. %HRmax, %MVPA and %VA were monitored during the protocols, while BLa, cortisol, and testosterone were measured before and after each protocol. CK was measured before the protocols and at 24 h, while RPE and enjoyment were assessed at the end of each protocol. 3 × 3BB elicited higher %HRmax (p < 0.001; d = -1.6, large), %MVPA (p < 0.001; d = 2.7, very large), %VA (p = 0.030; d = 0.8, moderate), enjoyment (p = 0.014; r-value = -0.500, large), and lower RPE (p = 0.024; r-value = -0.462, moderate) compared to the HIIT condition. Moreover, higher values of BLa were found in HIIT compared to 3 × 3BB at post-condition (p = 0.020; r-value = -0.601, large), while CK analysis showed only an increase within the HIIT condition (p = 0.020; r-value = -0.599, large). A time effect was found for both testosterone (p < 0.001, η2p = 0.526, moderate) and cortisol (p = 0.005, η2p = 0.743, strong), while no between-condition effect or interaction was found (p > 0.05). 3 × 3BB elicits higher %HRmax, enjoyment, and physical activity intensities but lower BLa and RPE compared to HIIT in active young adults and might be considered as a potentially suitable activity to increase participants' health status.

Metabolic and physiological demands of 3×3-min-round boxing fights in highly trained amateur boxers.

BACKGROUND: The aim of this study was to evaluate the energy requirements of the aerobic oxidative, anaerobic lactic (glycolytic), or anaerobic alactic systems in highly trained amateur boxers during a 3×3-min-round boxing fight. METHODS: On three separate occasions, 10 highly trained male amateur boxers undertook a progressive treadmill run, a progressive bag-punching exercise, and a full-contact competitive boxing fight of the 3×3-min format. Expired gas and heart rate (HR) were recorded throughout all exercise tasks, with the exception of a gas analysis during the competitive fight. Total energy expenditure and the contribution of the three major energy-supply systems were calculated based on oxygen uptake (V̇O2) and HR during exercise, blood lactate accumulation, and excess V̇O2 during the fast phase of the recovery. RESULTS: The blood lactate concentration was >15 (range: 12-18) mmol·L-1 after the fight. The HR reached >93% of the maximal (HRmax) in rounds 1 and 2 and >97% in the final round and was strongly correlated with HRmax (r=0.885). The average calculated O2 uptake during the boxing fight was 89% of V̇O2max, whereas O2 uptake equivalent calculated from the involvement of all three major energy supply systems was 122% of V̇O2max. The calculated energy provision during the fight was 73% aerobic, 19% anaerobic alactic and 8% anaerobic glycolytic. CONCLUSIONS: In highly trained male amateur boxers, 3×3-min boxing match elicits very high HR and blood lactate levels with predominant involvement of aerobic and substantial contribution of anaerobic alactic energy systems.

Increasing the resting time between drop jumps lessens delayed-onset muscle soreness and limits the extent of prolonged low-frequency force depression in human knee extensor muscles.

PURPOSE: Unaccustomed eccentric contractions generally result in a long-lasting contractile impairment, referred to as prolonged low-frequency force depression (PLFFD), and delayed-onset muscle soreness (DOMS). We here used repeated drop jumps (DJs) as an eccentric contraction model and studied the effects of increasing the time between DJs from 20 s to 5 min. We hypothesized that both PLFFD and DOMS would be less marked at the longer DJ interval due to the longer time to restore structural elements between DJs. METHODS: Young men (n = 12) randomly performed 50 DJs with either 20-s (DJ-20 s) or 5-min (DJ-5 min) rest between DJs. Voluntary, 20 Hz and 100 Hz electrically stimulated isometric knee extension torques and muscle soreness were monitored before and for 7 days after DJs; serum CK activity was measured to assess muscle fibre protein leakage. In additional experiments, changes in mRNA levels were assessed in muscle biopsies collected before and 1 h after exercise. RESULTS: A marked PLFFD was observed with both protocols and the extent of 20 Hz torque depression was smaller immediately and 1 day after DJ-5 min than after DJ-20 s (p < 0.05), whereas the MVC and 100 Hz torques were similarly decreased with the two protocols. Markedly larger differences between the two protocols were observed for the muscle soreness score, which 1-4 days after exercise was about two times larger with DJ-20 s than with DJ-5 min (p < 0.01). CONCLUSIONS: The larger protective effect of the longer DJ interval against DOMS than against PLFFD indicates that their underlying mechanisms involve different structural elements.

Adding High-Intensity Interval Training to Classical Resistance Training Does Not Impede the Recovery from Inactivity-Induced Leg Muscle Weakness.

Inactivity is known to induce muscle weakness, and chronically increased levels of reactive oxygen species (ROS) are proposed to have a central causative role in this process. Intriguingly, high-intensity interval training (HIIT), which involves bursts of high ROS production, can have positive effects in pathological conditions with chronically increased ROS. Here, young male volunteers were exposed to 3 weeks of unloading of the dominant leg followed by 3 weeks of resistance training without (Ctrl group) or with the addition of all-out cycling HIIT. Changes in muscle thickness were assessed by ultrasonography, and contractile function was studied by measuring the torque during maximal voluntary contractions (MVC). The results show an ~6% decrease in vastus lateralis thickness after the unloading period, which was fully restored after the subsequent training period in both the Ctrl and HIIT groups. MVC torque was decreased by ~11% after the unloading period and recovered fully during the subsequent training period in both groups. All-out cycling performance was improved by the 3 weeks of HIIT. In conclusion, the decline in muscle size and function after 3 weeks of unloading was restored by 3 weeks of resistance training regardless of whether it was combined with HIIT.

Cardiorespiratory Responses to Specific and Nonspecific Exercise in High-Profile Amateur Boxers.

ABSTRACT: Venckunas, T, Bruzas, V, Stasiulis, A, Snieckus, A, Mockus, P, and Kamandulis, S. Cardiorespiratory responses to specific and non-specific exercise in high-profile amateur boxers. J Strength Cond Res 36(3): 717-722, 2022-Our aim was to compare cardiorespiratory responses in 3 types of all-out exercises (upper body, lower body, and punching) in well-trained boxers and peak heart rate (HR) in these tests with HR response to competitive Olympic boxing. On 4 separate occasions, 11 male amateur boxers performed: (a) progressive treadmill running to exhaustion; (b) progressive arm cranking to failure; (c) progressive bag punching to maximal exertion; and (d) competitive boxing fight (3 × 3 minutes format). Expired gas and HR were measured in all tasks except in the full-contact competitive fight where only HR was recorded. Peak oxygen uptake (55.3 ± 5.9 ml·kg-1·min-1) was highest during treadmill running. Peak respiratory exchange ratio was highest during bag punching (1.26 ± 0.09), but did not differ between arm cranking and running. Peak HR of the boxing fight (197.7 ± 5.9 b·min-1) did not differ from that of running (196.5 ± 8.7 b·min-1), but was smaller in arm cranking (179.8 ± 9.2) and bag punching (184.1 ± 12.2 b·min-1). The correlation coefficients between peak HR achieved during competitive fighting and that achieved during running, arm cranking and bag punching were 0.720 (p = 0.013), 0.597 (p = 0.052), and 0.702 (p = 0.035), respectively. In conclusion, in high-profile amateur boxers, running evoked a higher cardiorespiratory activation than arm cranking or bag punching, whereas punching elicited the highest input of anaerobic glycolysis. Peak HR of competitive fighting was the same as during running and much higher than during arm cranking or bag punching.

Warm-Up and Hamstrings Stiffness, Stress Relaxation, Flexibility, and Knee Proprioception in Young Soccer Players.

CONTEXT: Nerves or fascia may limit motion in young soccer players, thereby contributing to frequent hamstrings injuries. Nerve-gliding exercises and self-myofascial release techniques may enhance range of motion (ROM). OBJECTIVE: To compare the immediate effect of foam rolling (FR) and neurodynamic nerve gliding (NDNG) on hamstrings passive stiffness, viscoelasticity, flexibility, and proprioception during the warm-up of soccer players. DESIGN: Crossover study. SETTING: Research laboratory. PATIENTS OR OTHER PARTICIPANTS: A total of 15 male soccer players (age = 18.0 ± 1.4 years, height = 183.1 ± 6.0 cm, mass = 76.9 ± 7.8 kg) on the same team. INTERVENTION(S): The FR and NDNG consisted of 6 sets of 45 seconds with a 15-second rest between sets. Over a 2-week period, participants performed FR and NDNG on 2 separate occasions. MAIN OUTCOME MEASURE(S): Stiffness (between 50% and 80%, and 85% and 95% [STFmax] of maximal knee-extension ROM), viscoelasticity (stress-relaxation test), knee-extension ROM, hamstrings passive-resistance torque (PRT), hip-flexion angle (straight-leg raise test), and active knee-joint position sense. RESULTS: We observed an interaction between time and intervention for STFmax (F1,17 = 5.024, P = .042), knee-extension ROM (F1,17 = 7.371, P = .02), and PRT (F1,17 = 4.876, P = .044). The NDNG technique induced increases in STFmax (t17 = 2.374, P = .03), ROM (t17 = 2.843, P = .01), and PRT (t17 = 2.982, P = .008). Both NDNG and FR led to improved performance on the straight-leg raise test (F1,17 = 87.514, P < .001). No interaction or main effect was found for the stress-relaxation test or active knee-joint position sense. CONCLUSIONS: Adding NDNG to the warm-up routine increased ROM more than FR and may benefit soccer players.

Endurance training-induced increase in muscle oxidative capacity without loss of muscle mass in younger and older resistance-trained men.

While concurrent training is regularly used in older populations, the inverse relationship between fibre size and oxidative capacity suggests that endurance training in resistance-trained individuals may result in some loss of resistance training-induced gains in muscle mass, which may be more pronounced in older people. We investigated the impact of superimposed endurance training in younger (28.5 ± 4.8 years; n = 8) and older (67.5 ± 5.5 years; n = 7) highly resistance-trained men. Participants underwent a 10-week endurance cycling training programme consisting of five 6-min intervals at 75% max heart rate (HRmax) separated by 4-min intervals at 90% HRmax. The anatomical cross-sectional area (ACSA) of the thigh muscles, as determined with MRI, was 24% smaller in older compared to younger participants (p < 0.001). Although maximal oxygen consumption (VO2max) was also lower in the older group (p < 0.001), VO2max per kg body mass did not differ significantly between younger and older participants. Histological analyses of biopsies of the m. vastus lateralis showed that endurance training induced an increase in succinate dehydrogenase activity in both younger and older participants (p ≤ 0.043), and an increase in the number of capillaries around type I fibres (p = 0.017). The superimposed endurance training did not induce a significant decrease in thigh ACSA, fibre cross-sectional area, or knee extensor maximum voluntary isometric force. These observations indicate that adding endurance training to resistance training can lead to positive endurance-related adaptations without negative consequences for muscle size and strength in older and younger resistance-trained people.

Response of Knee Extensor Muscle-Tendon Unit Stiffness to Unaccustomed and Repeated High-Volume Eccentric Exercise.

The purposes of this study were to investigate the muscle-tendon unit stiffness response and to compare the stiffness with those of other indirect markers induced by two bouts of unaccustomed eccentric exercise. Eleven untrained men performed two bouts of 200 maximal eccentric contractions of the right quadriceps 4 weeks apart. Changes in stiffness, pain evoked by stretching and pressure, plasma creatine kinase (CK) activity, and muscle thickness were followed for 7 days after each bout. Stiffness and pain peaked immediately and 1 day after the first exercise bout, whereas CK and thickness were highest 4 and 7 days after the first exercise bout, respectively (p < 0.05 for all). Muscular pain, thickness, and stiffness responses were lower by 53.3%, 99%, and 11.6%, respectively, after the repeated bout compared to after the first bout (p < 0.05 for all), while CK activity response did not differ significantly between bouts. High responders for an increase in muscle-tendon unit stiffness showed a repeated-bout effect for stiffness, pain, and CK activity (by 29%, 65%, and 98%, p < 0.05 for all), but the repeated-bout effect was not that clear in low responders. These findings suggest that a repeated eccentric exercise bout effect on stiffness in quadriceps is mostly not associated with muscle pain and CK activity, but there are large individual differences.

Effect of high volume stretch-shortening cycle exercise on vertical leg stiffness and jump performance.

The study aims were to investigate the effects of muscle damage induced by high-volume stretch-shortening cycle exercise on countermovement jump (CMJ) performance and vertical leg stiffness during squat and landing phases. Changes in the peak torque of knee extensor muscles, soreness, plasma CK activity, vertical leg stiffness, and CMJ characteristics were measured in recreationally active men after 50, 100, 150 and 200 drop jumps (DJs) and again 24 h later. The isometric voluntary peak torque after 50 DJs decreased by 22 ± 12% (p < 0.01). However, during the squat phase of CMJ eccentric peak power and rate of force development together with the concentric mean force during the push-off phase increased after 50 DJs (all p < 0.05). After 200 DJs, jump height and concentric peak power during push-off were decreased (p < 0.05). At 24 h after exercise, jump height, peak eccentric force, the rate of eccentric force development and the mean concentric force in CMJ and vertical leg stiffness in the squat all remained decreased (p < 0.05), while vertical leg stiffness in the landing phase was increased (p < 0.05). Muscle damaging exercise volume is associated with changes in vertical leg stiffness and CMJ performance.

Effect of Training and Match Loads on Hamstring Passive Stiffness in Professional Soccer Players.

OBJECTIVE: the purpose of this study was to identify differences in hamstring passive stiffness between the pre-season and in-season periods. METHODS: Hamstring strength and passive stiffness were measured in professional male soccer players before and after the pre-season (4 weeks), and after the in-season (6 weeks) periods using an isokinetic dynamometer. Muscle passive stiffness was determined from the slope of the passive torque-angle relationship. External loads (acceleration and jumps) were monitored by GPS and internal loads by questionnaire. RESULTS: Hamstring passive stiffness increased after 10 weeks of training and matches, without changes in passive peak torque and range of motion. The hamstring passive stiffness modifications were associated with the volume and intensity of accelerations and jumps. The individual data analysis also provided some support for the suppression of the biomechanical adaptation in the subjects with relatively large external load. CONCLUSIONS: Regular training and match workouts increase hamstring passive stiffness in professional soccer players but the adaptation of muscle-tendon unit passive elements might not occur if players experience excessive mechanical stress.

Vitamin C and E Treatment Blunts Sprint Interval Training-Induced Changes in Inflammatory Mediator-, Calcium-, and Mitochondria-Related Signaling in Recreationally Active Elderly Humans.

Sprint interval training (SIT) has emerged as a time-efficient training regimen for young individuals. Here, we studied whether SIT is effective also in elderly individuals and whether the training response was affected by treatment with the antioxidants vitamin C and E. Recreationally active elderly (mean age 65) men received either vitamin C (1 g/day) and vitamin E (235 mg/day) or placebo. Training consisted of nine SIT sessions (three sessions/week for three weeks of 4-6 repetitions of 30-s all-out cycling sprints) interposed by 4 min rest. Vastus lateralis muscle biopsies were taken before, 1 h after, and 24 h after the first and last SIT sessions. At the end of the three weeks of training, SIT-induced changes in relative mRNA expression of reactive oxygen/nitrogen species (ROS)- and mitochondria-related proteins, inflammatory mediators, and the sarcoplasmic reticulum Ca2+ channel, the ryanodine receptor 1 (RyR1), were blunted in the vitamin treated group. Western blots frequently showed a major (>50%) decrease in the full-length expression of RyR1 24 h after SIT sessions; in the trained state, vitamin treatment seemed to provide protection against this severe RyR1 modification. Power at exhaustion during an incremental cycling test was increased by ~5% at the end of the training period, whereas maximal oxygen uptake remained unchanged; vitamin treatment did not affect these measures. In conclusion, treatment with the antioxidants vitamin C and E blunts SIT-induced cellular signaling in skeletal muscle of elderly individuals, while the present training regimen was too short or too intense for the changes in signaling to be translated into a clear-cut change in physical performance.

Response to Three Weeks of Sprint Interval Training Cannot Be Explained by the Exertional Level.

Background and Objectives: The all-out mode of sprint interval training (SIT) has been shown to be an efficient method for improving sports performance, exercise capacity, and aerobic fitness. Although the benefits of SIT are well described, the mechanisms underlying the different degrees of response remain largely unexplored. We aimed to assess the effects of exertion on the responsiveness to SIT. Materials and Methods: The participants were 28 young untrained men (mean ± SD age 25.7 ± 6.03 years) who exhibited either a large or small increase in Wingate test average power in response to nine SIT sessions performed over three weeks. Each training session comprised four-six bouts of 30 s all-out cycling interspaced with 4 min of rest. Individual responses were assessed using heart rate (HR) during exercise for all nine sessions, as well as blood lactate concentration up to 1 h, and the decrement in maximal voluntary knee extension torque (MVC) up to 24 h after the first and last training sessions. Peak oxygen uptake (VO2peak) and maximum HR were measured before and after training during an incremental cycling test to exhaustion. Results: Although all participants showed benefits of SIT such as increased VO2peak, the increase in anaerobic cycling power varied between participants. We identified 17 high responders and nine low responders, whose average power outputs were 0.80 ± 0.22 and 0.22 ± 0.19 W/kg, respectively. The HR achieved during any of the training sessions did not differ between high and low responders. The lactate kinetics did not differ between groups before and after the intervention. Training resulted in a more rapid recovery of MVC without any discernible differences between the high and low responders. Conclusion: The differences in the responses to SIT are not dependent on the exertion level during training.

Daily Resting Heart Rate Variability in Adolescent Swimmers during 11 Weeks of Training.

Adolescent athletes are particularly vulnerable to stress. The current study aimed to monitor one of the most popular and accessible stress markers, heart rate variability (HRV), and its associations with training load and sleep duration in young swimmers during an 11-week training period to evaluate its relevance as a tool for monitoring overtraining. National-level swimmers (n = 22, age 14.3 ± 1.0 years) of sprint and middle distance events followed individually structured training programs prescribed by their swimming coach with the main intention of preparing for the national championships. HRV after awakening, during sleep and training were recorded daily. There was a consistent ~4.5% reduction in HRV after 3-5 consecutive days of high (>6 km/day) swimming volume, and an inverse relationship of HRV with large (>7.0 km/day) shifts in total training load (r = -0.35, p < 0.05). Day-to-day HRV did not significantly correlate with training volume or sleep duration. Taken together, these findings suggest that the value of HRV fluctuations in estimating the balance between the magnitude of a young athlete's physical load and their tolerance is limited on a day-to-day basis, while under sharply increased or extended training load the lower HRV becomes an important indicator of potential overtraining.

Low frequency fatigue and changes in muscle fascicle length following eccentric exercise of the knee extensors.

NEW FINDING: What is the central question of this study? Does low frequency muscle fatigue indicate a failure of excitation-contraction coupling after eccentric exercise, or is it simply due to a change in muscle length? What is the main finding and its importance? The low to high frequency muscle fatigue ratio was relatively insensitive to changes in muscle length, and any changes in length following eccentric exercise were far too small to account for the high degree of low frequency fatigue. The results strengthen the suggestion that the early loss of force following eccentric exercise is due to a deficit of excitation-contraction coupling. ABSTRACT: Development of long lasting fatigue (low frequency fatigue; LFF), assessed as the ratio of forces at 20 and 100 Hz stimulation, suggests the early phase of muscle damage caused by eccentric exercise is due to a deficit of excitation-contraction coupling. However, this could be caused by a change of muscle length. Eleven men (21.3 ± 2.0 years) performed 200 maximum eccentric knee extensions (30-110 deg flexion). Force generated by 20 and 100 Hz stimulation and maximum isometric force (MIF) were determined at knee angles 50, 70 and 90 deg before and immediately after the exercise. Vastus lateralis fascicle length (FL) was measured by ultrasound of resting and contracting muscle. Peak MIF (829 ± 119 N) was at 70 deg knee flexion, falling to 486 ± 180 N (P < 0.001) after exercise, but with no change in optimum angle. FLs at rest were unaffected by eccentric exercise, but during contraction they were on average 8.8% (95% CI: 4.1, 13.5%, P = 0.002) longer after exercise. Before exercise, the 20/100 ratio increased with muscle length, from 0.69 ± 0.09 at 50 deg, 0.72 ± 0.05 at 70 deg and 0.80 ± 0.08 at knee angle 90 deg (P < 0.001). After eccentric exercise the 20/100 ratio was reduced to 0.29 ± 0.08 at 50 deg, 0.27 ± 0.04 at 70 deg and 0.34 ± 0.04 at 90 deg (P < 0.001). The 20/100 ratio was relatively insensitive to changes in muscle length and the decrease following eccentric exercise was far greater than might be caused by any changes in muscle length after eccentric exercise. The results show that LFF following eccentric exercise is not due to change in muscle length and strengthen the suggestion that it represents a deficit in excitation-contraction coupling.