RESUMO
The ELISA is the most worldwide method for immunoassay. However, the ELISA is losing ground due to low reproducibility of manual experimental processes in both R&D and IVD areas. An automated platform is a good solution, but there are still limitations owning to extremely high cost and requiring large space to set up especially for a small size laboratory. Here, we present a novel all-in-one platform called "VEUS" settable on the laboratory table that offers comprehensive automation of the entire multiplex immunoassay process by exploiting antibody conjugated magnetic particles, quality control and then immunoanalytical reaction, thereby enhancing detection sensitivity and high reproducibility. As a proof of concept, the system exhibits a sensitive LOD of 0.6 and 3.1 pg mL-1 within 1 h run, comparable precision that of molecular diagnostic systems based on PCR method, enabling rapid multiplex diagnosis of Influenza A, Influenza B, and COVID-19 viruses with similar symptoms. Through automation by the all-in-one system, it can be used by novice users, something innovative for immunoassays, relying heavily on user experience. Furthermore, it can contribute to streamline entire immunoassay processes of diverse biomarkers with high reproducibility and convenience in laboratories.
Assuntos
SARS-CoV-2 , Humanos , Imunoensaio/métodos , SARS-CoV-2/imunologia , SARS-CoV-2/isolamento & purificação , Anticorpos Imobilizados/imunologia , Anticorpos Imobilizados/química , Reprodutibilidade dos Testes , COVID-19/diagnóstico , COVID-19/virologia , Ensaio de Imunoadsorção Enzimática/métodos , Vírus da Influenza A/imunologia , Vírus da Influenza B/imunologia , Automação Laboratorial/métodos , Limite de DetecçãoRESUMO
DNA-based data storage has attracted attention because of its higher physical density of the data and longer retention time than those of conventional digital data storage. However, previous DNA-based data storage lacked index features and the data quality of storage after a single access was not preserved, obstructing its industrial use. Here, DNA micro-disks, QR-coded micro-sized disks that harbor data-encoded DNA molecules for the efficient management of DNA-based data storage, are proposed. The two major features that previous DNA-based data-storage studies could not achieve are demonstrated. One feature is accessing data items efficiently by indexing the data-encoded DNA library. Another is achieving write-once-read-many (WORM) memory through the immobilization of DNA molecules on the disk and their enrichment through in situ DNA production. Through these features, the reliability of DNA-based data storage is increased by allowing selective and multiple accession of data-encoded DNA with lower data loss than previous DNA-based data storage methods.
Assuntos
Dispositivos de Armazenamento em Computador , DNA , Armazenamento e Recuperação da Informação/métodosRESUMO
DNA-based data storage has emerged as a promising method to satisfy the exponentially increasing demand for information storage. However, practical implementation of DNA-based data storage remains a challenge because of the high cost of data writing through DNA synthesis. Here, we propose the use of degenerate bases as encoding characters in addition to A, C, G, and T, which augments the amount of data that can be stored per length of DNA sequence designed (information capacity) and lowering the amount of DNA synthesis per storing unit data. Using the proposed method, we experimentally achieved an information capacity of 3.37 bits/character. The demonstrated information capacity is more than twice when compared to the highest information capacity previously achieved. The proposed method can be integrated with synthetic technologies in the future to reduce the cost of DNA-based data storage by 50%.
Assuntos
DNA/genética , Bases de Dados de Ácidos Nucleicos , Armazenamento e Recuperação da Informação , Sequência de Bases/genética , Análise de Sequência de DNARESUMO
BACKGROUND: Pen-based devices have emerged as useful tools for measuring pH and glucose, and for fabricating microchannels and microarrays. Pen-based devices take advantage of flexible patterning, inexpensive costs, and small volumes, thereby saving time and increasing efficiency. We have developed a gradient nib marker pen device that generated simultaneously different antibiotic concentrations in bacteria antibiotic susceptibility testing (AST). METHODS: The device can deposit on the target surface with the antibiotic gradient. The designed polyester fiber nibs are a highly uniform porosity with unidirectional orientation and produce a visible gradient pattern. RESULTS: We have demonstrated and quantitatively analyzed bacterial growth after antibiotic marking. The antibiotic marking produces an inhibition zone of bacterial growth. The inhibition zones of bacterial growth are captured and converted to 8-bit grayscale images, and then quantified by gray values using the Image J program. A profile of the inhibition zone showed different gray values in response to bacterial viability. CONCLUSION: The gradient nib marker pen device can be used to determine the quantitative antibiotic concentration based on the relationship between gray values and bacterial density conveniently without requiring a series of dilution tubes, including nutrient medium, and diversely diluted antibiotics.
Assuntos
Antibacterianos/farmacologia , Bactérias , Testes de Sensibilidade MicrobianaRESUMO
An unclonable, fingerprint-mimicking anti-counterfeiting strategy is presented that encrypts polymeric particles with randomly generated silica film wrinkles. The generated wrinkle codes are as highly unique as human fingerprints and are technically irreproducible. Superior to previous physical unclonable functions, codes are tunable on demand and generable on various geometries. Reliable authentication of real-world products that have these microfingerprints is demonstrated using optical decoding methods.
Assuntos
Biomimética/métodos , Dermatoglifia , Fraude/prevenção & controle , Humanos , Fenômenos Mecânicos , Microesferas , Dióxido de SilícioRESUMO
This article presents free-floating three-dimensional (3D) microstructure fabrication in a microfluidic channel using direct fine-tuned grayscale image lithography. The image is designed as a freeform shape and is composed of gray shades as light-absorbing features. Gray shade levels are modulated through multiple reflections of light in a digital micromirror device (DMD) to produce different height formations. Whereas conventional photolithography has several limitations in producing grayscale colors on photomask features, our method focuses on a maskless, single-shot process for fabrication of freeform 3D micro-scale shapes. The fine-tuned gray image is designed using an 8-bit grayscale color; thus, each pixel is capable of displaying 256 gray shades. The pattern of the UV light reflecting on the DMD is transferred to a photocurable resin flowing through a microfluidic channel. Here, we demonstrate diverse free-floating 3D microstructure fabrication using fine-tuned grayscale image lithography. Additionally, we produce polymeric microstructures with locally embedded gray encoding patterns, such as grayscale-encoded microtags. This functional microstructure can be applied to a biophysical detection system combined with 3D microstructures. This method would be suitable for fabricating 3D microstructures that have a specific morphology to be used for particular biological or medical applications.
RESUMO
Aggregation and adhesion of platelets to the vascular wall are shear-dependent processes that play critical roles in hemostasis and thrombosis at vascular injury sites. In this study, we designed a simple and rapid assay of platelet aggregation and adhesion in a microfluidic system. A shearing mechanism using a rotating stirrer provided adjustable shear rate and shearing time and induced platelet activation. When sheared blood was driven through the microchannel under vacuum pressure, shear-activated platelets adhered to a collagen-coated surface, causing blood flow to significantly slow and eventually stop. To measure platelet adhesion and aggregation, the migration distance (MD) of blood through the microchannel was monitored. As the microstirrer speed increased, MD initially decreased exponentially but then increased beyond a critical rpm. For platelet-excluded blood samples, there were no changes in MD with increasing stirrer speed. These findings imply that the stirrer provided sufficiently high shear to activate platelets and that blood MD is a potentially valuable index for measuring the shear-dependence of platelet activation. Our microfluidic system is quick and simple, while providing a precise assay to measure the effects of shear on platelet aggregation and adhesion.
RESUMO
Wireless signal transmission will play a critical role in developing reliable subdural spinal cord stimulation systems. We have developed an approach to inductively coupling signals across the epidural spacing between the pial and epidural surfaces. The major design constraints include tolerance of coil misalignments from spinal cord geometries in addition to reasonable power efficiencies within the expected range of movement. The design of the primary side as a driving circuit is simplified by several turns of commercial magnetic wire, whereas the implanted secondary side is implemented in a micro-planar spiral coil tuned to a resonant frequency of 1.6 MHz. We present the results of wireless inductive coupling experiments that demonstrate the ability to transmit and receive a frequency modulated 1.6 MHz carrier signal between primary and secondary coil antennae scaled to 10 mm. Power delivery is in the range of 400 mW at a link efficiency of 32 % for strong coupling (coil separations of 0.5 mm ) and in the range of 70 mW at 4 % efficiency for weak coupling (coil separations of 10 mm).
Assuntos
Eletricidade , Estimulação da Medula Espinal/instrumentação , Espaço Subdural , Desenho de Equipamento , Tecnologia sem FioRESUMO
Inductively coupled planar resonators offer convenient integration of high-resolution NMR spectroscopy with microfluidic lab-on-a-chip devices. Planar spiral resonators are fabricated lithographically either by gold electroplating or by etching Cu laminated with polyimide. Their performance is characterized by NMR imaging as well as spectroscopy. A single-scan limit of detection LOD(t) = 0.95 nmol s(1/2) was obtained from sample volumes around 1 µL. The sensitivity of this approach is similar to that obtained by microstripline and microslot probes.
Assuntos
Espectroscopia de Ressonância Magnética , Técnicas Analíticas Microfluídicas , Limite de Detecção , Técnicas Analíticas Microfluídicas/instrumentação , Técnicas Analíticas Microfluídicas/métodosRESUMO
We developed a novel microfluidic cell culture device in which magnetic beads repetitively collide with osteoblast cells, MC3T3-E1, owing to attractive forces generated by pulsed electromagnetic fields and consequently the cells were physically stimulated by bead impacts. Our device consists of an on-chip microelectromagnet and a microfluidic channel which were fabricated by a microelectromechanical system technique. The impact forces and stresses acting on a cell were numerically analyzed and experimentally generated with different sizes of bead (4.5, 7.6 and 8.4 mum) and at various pulse frequencies (60 Hz, 1 kHz and 1 MHz). Cells were synchronized at each specific phase of the cell cycle before stimulation in order to determine the most susceptible phase against bead impacts. The cells were stimulated with different sizes of bead at various pulse frequencies for 1 min at G1, S and G2 phases, respectively, and then counted immediately after one doubling time. The growth rate of cells was highly accelerated when they were stimulated with 4.5 mum beads at G1 phase and a pulse frequency of 1 MHz. Almost all of the cells were viable after stimulation, indicating that our cell stimulator did not cause any cellular damage and is suitable for use in new physical stimulus modalities.
Assuntos
Magnetismo , Osteoblastos/fisiologia , Estimulação Física/métodos , Células 3T3/citologia , Células 3T3/fisiologia , Animais , Contagem de Células , Técnicas de Cultura de Células/métodos , Ciclo Celular , Divisão Celular , Sobrevivência Celular , Replicação do DNA , Campos Eletromagnéticos , Citometria de Fluxo/métodos , Humanos , Cinética , Camundongos , Microfluídica , Microscopia de Força Atômica , Osteoblastos/citologia , Osteoblastos/ultraestrutura , Estimulação Física/instrumentaçãoRESUMO
Blood hemoglobin is an important diagnostic parameter in measuring overall health. The hemoglobin molecule and the iron it contains absorb light energy, leading to thermal changes. This paper presents a new method for determining the hemoglobin concentration of erythrocytes by measuring temperature increases of the heme group when cells are heated by a 532 nm wavelength laser. The advantages of our method are that it determines the hemoglobin content of an entire blood sample without chemical treatments and requires only a small amount of blood (less than 10 microL). A micro scaled platinum resistance temperature detector (Pt RTD) was fabricated using a microelectromechanical system (MEMS) technique that directly measures the temperature changes. The platinum RTD's resistance at 0 degrees C is 275.32 Omega. For the specific heating of erythrocytes, we used a 0.03 to 9.6 W cm(-2) power tunable diode pumped solid state (DPSS) continuous wave (CW) laser module with a wavelength of 532 nm. When heating human erythrocytes, leukocytes, plasma, and reference solutions, only the temperature of the erythrocytes significantly increased, indicating that our measurement technique can be used to determine hemoglobin concentration. The hemoglobin concentrations for the samples we used were 0.34, 0.67, 1.35, 2.7, 5.4, 8.1, 10.8, 13.5, 16.2, 18.9 and 21.6 g dL(-1). The temperatures measured for each sample were 31.17 +/- 1.98, 36.34 +/- 3.76, 42.70 +/- 4.38, 48.39 +/- 6.47, 63.73 +/- 3.34, 79.09 +/- 9.60, 84.86 +/- 1.99, 87.54 +/- 9.84, 91.90 +/- 5.27, 90.00 +/- 3.24 and 95.79 +/- 2.66 degrees C at a 9.6 W cm(-2) output power of the 532 nm laser at 23 degrees C. We also provide a theoretical analysis of the temperature increases and investigate their major heat source.
Assuntos
Eritrócitos/metabolismo , Heme/química , Hemoglobinas/análise , Eritrócitos/imunologia , Humanos , Lasers , Platina/química , TemperaturaRESUMO
We developed a new microfluidic method for focusing microparticles through the combined use of inertial lift forces and turbulent secondary flows generated in a topographically patterned microchannel. The mechanism of particle focusing is based on the hydrodynamic inertial forces exerted on particles migrating along a non-circular microchannel, i.e.tubular pinch effect and wall effect, which induce particle movement away from walls and along a specific lateral position in the microchannel. With the extraordinary geometry of multi-orifice microchannel, an ordered and focused particle distribution was achieved at central or side regions according to a particle Reynolds number (Re(p)) range. The focusing of particles was controlled by the particle Reynolds number, microchannel length, and volume fraction of particles in suspension. This method will be beneficial in particle focusing processes in a microfluidic device since it offers continuous, high-throughput performance and simple operation.