RESUMO
OBJECTIVE: The aim of the present study was to investigate the possible effects of the +83C/T polymorphism in apolipoprotein AI gene (apoA1) on the changes of serum lipids, glucose, insulin and the homeostasis model assessment of insulin resistance (HOMA-IR) induced by a high-carbohydrate diet in healthy youth. METHODS: Fifty-six participants were given a washout diet for 7 d, followed by a high-carbohydrate diet for 6 d. The washout diet contained 15% protein, 31% fat and 54% carbohydrate. The high-carbohydrate diet contained 15% protein, 15% fat and 70% carbohydrate. Twelve hour fasting venous blood was drawn on the mornings of the first, eighth and fourteenth days. Blood lipids, glucose and insulin were measured, and HOMA-IR was calculated. The genome DNA was extracted and the apoA1 + 83C/T polymorphism was determined by polymerase chain reaction-based restriction fragment length polymorphism. RESULTS: Triglyceride and insulin were found significantly increased in the subjects with the CC genotype, but not in the T carriers after the high-carbohydrate diet. Significant decreases of total cholesterol and LDL-C and a significant increase of HDL-C were observed after the dietary intervention of the high-carbohydrate diet. CONCLUSION: The triglyceride and insulin changes after the high-carbohydrate diet can be modulated by the apoA1 +83C/T polymorphism, and the T allele may eliminate the increase in triglyceride and insulin induced by the high-carbohydrate diet.
