Palynological Investigations of the Miocene sediments from Murree formation of Pakistan: Evidence for Palaeoenvironment and Palaeoclimate interpretations.

The present study was carried out to investigate the palynoflora of Murree formation using microscopic techniques to understand the climatic changes and vegetation's evolution. In this palynological study, 31 samples were collected, analyzed, and then identified as palynomorphs using different previous published literature. The results of this study will be described in terms of the evolutionary history of plants and the depositional environment of the reported taxa in the study area. The botanical affinities and systematic description of the taxa were examined using light and scanning electron microscopy. The floral record identified Asteraceae as the dominant family and Pinus as a genus. Most of angiosperms i.e Fabaceae and Poaceae, have a poor fossil record but have an abundant palynological record in the study area. The highest polar diameter (75.75 µm), colpus length (34.5 µm) and colpus width (31 µm) were examined for the Convolvulus. Most of the taxa explained here had a wide geographical occurrence in Southeast Asia and show the abundance of angiosperms in the Miocene epoch. The palynological record of Holocene samples is important to know about the vegetation's origin and environmental fluctuations in the study area.

Phyto-ecological studies and distribution patterns of subfamily Polygonoideae in relation to edaphic factors across diverse ecological zones.

The species of the subfamily Polygonoideae is an essential component of temperate forests as well as the flora of the western Himalayan region. The aim of this research was to explore the taxonomic diversity, distribution patterns, and associated flora of Polygonoideae in relation to edaphic factors in various ecological zones in the Muzaffarabad division of the Kashmir Western Himalayan Region. We applied a random sampling approach for data collection from 10 different sites with a cumulative 780 quadrats to record the diversity of wild Polygonoideae species across the Muzaffarabad division between 2021 and 2022. This study revealed 279 plant species from 192 genera and 75 families associated with Polygonoideae, with the dominant families being Asteraceae, Poaceae, Lamiaceae, and Rosaceae. Herbs were predominant in the investigated area, with a proportion of 72.40 %, followed by shrubs (9.68 %) and pteridophytes (8.24 %). The flora was dominated by therophytes (37.35 %), whereas nanophylls (37.28 %) were the most dominant leaf form. Persicaria, Rumex, and Polygonum genera were observed and collected from various ecological zones, while Bistorta, Fagopyrum, Oxyria, and Rheum were only collected from a single zone, representing a restricted niche. A total of 28 taxa from 8 genera were studied in the investigative subfamily Polygonoideae, with the majority being therophytes (57.14 %), followed by hemicryptophytes (28.57 %), and leaf form dominated by microphylls (50 %) and nanophylls (17.85 %). The average values for Shannon and Simpson's diversity for the reported plant communities were 0.96 and 3.53, respectively, whereas species richness averaged 2.43 and species evenness 0.92. The vegetation exhibited a relatively lower (<50) maturity, averaging 32.08. Deforestation, overexploitation for medicinal purposes, soil erosion, overgrazing, forest fires, and the expansion of agricultural fields were identified as major threats to floral diversity. A significant correlation was observed between elevation and soil nutrient parameters, where moisture content, SOC, SOM, TK, and TN ratios showed a positive correlation, while pH and TP showed a negative correlation. Polygonum paronychioides and Rumex alpinus were the least observed of Polygonoideae taxa, whereas 39 species were found to be threatened, having low (<0.2) IVI values and seeking immediate conservation efforts. Climate change and anthropogenic pressure may lead to a change in the composition patterns and threaten the Polygonoidae species. We suggest community-based initiatives and sustainable conservation measures to safeguard the floral wealth of the Western Himalaya.

Systematic Implications of Palynomorph Diversity Using Microscopic Trends Among Asteraceous Flora From the Drylands of Baluchistan, Pakistan.

Pollen micromorphological traits with taxonomic implications are first reported from the study area for 50 Asteraceous taxa belonging to nine tribes. Cichorieae (21 taxa), Cardueae (11 taxa), Inuleae (six taxa), and Anthemideae (four taxa) are the leading tribes. The research included Cousinia haeckeliae, Himalaiella afghana, Pterachaenia stewartii (endemic to Afghanistan and Pakistan), and Xylanthemum macropodum (endemic to Baluchistan). Light and scanning electron microscopy were employed for the visualization of pollen photomicrographs. The data was analyzed statistically via SPSS, PAST, and Origin. Significant diagnostic qualitative and quantitative palynological traits were explored for discrimination down to the species level within the tribes. All the investigated taxa possessed radial symmetry, isopolarity, and monad form (characters for distinction at the subdivision level). The aperture types were trizonocolporate, tetrazonocolporate, and tricolporate with number position and character (NPC) formulas N3P4C5, N4P4C5, and N3P4C3. Goniotreme, peritreme, and ptychotreme types of amb were recognized. Echinate, echinate lophate, scabrate, and gemmate sculpturing were present with and without perforated surface patterns. Variations in the shapes in polar and equatorial views and lacuna shapes further assisted the separation of taxa. The observed shape classes were perprolate, prolate spheroidal, prolate, subprolate, oblate spheroidal, suboblate, and oblate. Principal component analysis, correlation, standard probability plots, and ridge line paired features plot for quantitative variables determined the positive correlation between the length and width of colpi in equatorial and polar view with polar axis and equatorial diameter and number of spines between colpi with the number of spines per pollen. The number of spines per pollen was negatively correlated with the width and length of colpi in the polar view. Multiple sample analysis of variance (ANOVA) concluded that a high statistically significant difference exists among the means of analyzed traits. The examined qualitative and quantitative palynological traits revealed noticeable variations, thus providing the source for species discrimination in Asteraceous tribes.

Data Management in Multicountry Consortium Studies: The Enterics For Global Health (EFGH) Shigella Surveillance Study Example.

Background: Rigorous data management systems and planning are essential to successful research projects, especially for large, multicountry consortium studies involving partnerships across multiple institutions. Here we describe the development and implementation of data management systems and procedures for the Enterics For Global Health (EFGH) Shigella surveillance study-a 7-country diarrhea surveillance study that will conduct facility-based surveillance concurrent with population-based enumeration and a health care utilization survey to estimate the incidence of Shigella--associated diarrhea in children 6 to 35 months old. Methods: The goals of EFGH data management are to utilize the knowledge and experience of consortium members to collect high-quality data and ensure equity in access and decision-making. During the planning phase before study initiation, a working group of representatives from each EFGH country site, the coordination team, and other partners met regularly to develop the data management systems for the study. Results: This resulted in the Data Management Plan, which included selecting REDCap and SurveyCTO as the primary database systems. Consequently, we laid out procedures for data processing and storage, study monitoring and reporting, data quality control and assurance activities, and data access. The data management system and associated real-time visualizations allow for rapid data cleaning activities and progress monitoring and will enable quicker time to analysis. Conclusions: Experiences from this study will contribute toward enriching the sparse landscape of data management methods publications and serve as a case study for future studies seeking to collect and manage data consistently and rigorously while maintaining equitable access to and control of data.

Diarrhea Case Surveillance in the Enterics for Global Health Shigella Surveillance Study: Epidemiologic Methods.

Background: Shigella is a leading cause of acute watery diarrhea, dysentery, and diarrhea-attributed linear growth faltering, a precursor to stunting and lifelong morbidity. Several promising Shigella vaccines are in development and field efficacy trials will require a consortium of potential vaccine trial sites with up-to-date Shigella diarrhea incidence data. Methods: The Enterics for Global Health (EFGH) Shigella surveillance study will employ facility-based enrollment of diarrhea cases aged 6-35 months with 3 months of follow-up to establish incidence rates and document clinical, anthropometric, and financial consequences of Shigella diarrhea at 7 country sites (Mali, Kenya, The Gambia, Malawi, Bangladesh, Pakistan, and Peru). Over a 24-month period between 2022 and 2024, the EFGH study aims to enroll 9800 children (1400 per country site) between 6 and 35 months of age who present to local health facilities with diarrhea. Shigella species (spp.) will be identified and serotyped from rectal swabs by conventional microbiologic methods and quantitative polymerase chain reaction. Shigella spp. isolates will undergo serotyping and antimicrobial susceptibility testing. Incorporating population and healthcare utilization estimates from contemporaneous household sampling in the catchment areas of enrollment facilities, we will estimate Shigella diarrhea incidence rates. Conclusions: This multicountry surveillance network will provide key incidence data needed to design Shigella vaccine trials and strengthen readiness for potential trial implementation. Data collected in EFGH will inform policy makers about the relative importance of this vaccine-preventable disease, accelerating the time to vaccine availability and uptake among children in high-burden settings.

Exploring Natural Immune Responses to Shigella Exposure Using Multiplex Bead Assays on Dried Blood Spots in High-Burden Countries: Protocol From a Multisite Diarrhea Surveillance Study.

Background: Molecular diagnostics on human fecal samples have identified a larger burden of shigellosis than previously appreciated by culture. Evidence of fold changes in immunoglobulin G (IgG) to conserved and type-specific Shigella antigens could be used to validate the molecular assignment of type-specific Shigella as the etiology of acute diarrhea and support polymerase chain reaction (PCR)-based microbiologic end points for vaccine trials. Methods: We will test dried blood spots collected at enrollment and 4 weeks later using bead-based immunoassays for IgG to invasion plasmid antigen B and type-specific lipopolysaccharide O-antigen for Shigella flexneri 1b, 2a, 3a, and 6 and Shigella sonnei in Shigella-positive cases and age-, site-, and season-matched test-negative controls from all sites in the Enterics for Global Health (EFGH) Shigella surveillance study. Fold antibody responses will be compared between culture-positive, culture-negative but PCR-attributable, and PCR-positive but not attributable cases and test-negative controls. Age- and site-specific seroprevalence distributions will be identified, and the association between baseline antibodies and Shigella attribution will be estimated. Conclusions: The integration of these assays into the EFGH study will help support PCR-based attribution of acute diarrhea to type-specific Shigella, describe the baseline seroprevalence of conserved and type-specific Shigella antibodies, and support correlates of protection for immunity to Shigella diarrhea. These insights can help support the development and evaluation of Shigella vaccine candidates.

Population Enumeration and Household Utilization Survey Methods in the Enterics for Global Health (EFGH): Shigella Surveillance Study.

Background: Accurate estimation of diarrhea incidence from facility-based surveillance requires estimating the population at risk and accounting for case patients who do not seek care. The Enterics for Global Health (EFGH) Shigella surveillance study will characterize population denominators and healthcare-seeking behavior proportions to calculate incidence rates of Shigella diarrhea in children aged 6-35 months across 7 sites in Africa, Asia, and Latin America. Methods: The Enterics for Global Health (EFGH) Shigella surveillance study will use a hybrid surveillance design, supplementing facility-based surveillance with population-based surveys to estimate population size and the proportion of children with diarrhea brought for care at EFGH health facilities. Continuous data collection over a 24 month period captures seasonality and ensures representative sampling of the population at risk during the period of facility-based enrollments. Study catchment areas are broken into randomized clusters, each sized to be feasibly enumerated by individual field teams. Conclusions: The methods presented herein aim to minimize the challenges associated with hybrid surveillance, such as poor parity between survey area coverage and facility coverage, population fluctuations, seasonal variability, and adjustments to care-seeking behavior.

Quantifying the Cost of Shigella Diarrhea in the Enterics for Global Health (EFGH) Shigella Surveillance Study.

Background: Comparative costs of public health interventions provide valuable data for decision making. However, the availability of comprehensive and context-specific costs is often limited. The Enterics for Global Health (EFGH) Shigella surveillance study-a facility-based diarrhea surveillance study across 7 countries-aims to generate evidence on health system and household costs associated with medically attended Shigella diarrhea in children. Methods: EFGH working groups comprising representatives from each country (Bangladesh, Kenya, Malawi, Mali, Pakistan, Peru, and The Gambia) developed the study methods. Over a 24-month surveillance period, facility-based surveys will collect data on resource use for the medical treatment of an estimated 9800 children aged 6-35 months with diarrhea. Through these surveys, we will describe and quantify medical resources used in the treatment of diarrhea (eg, medication, supplies, and provider salaries), nonmedical resources (eg, travel costs to the facility), and the amount of caregiver time lost from work to care for their sick child. To assign costs to each identified resource, we will use a combination of caregiver interviews, national medical price lists, and databases from the World Health Organization and the International Labor Organization. Our primary outcome will be the estimated cost per inpatient and outpatient episode of medically attended Shigella diarrhea treatment across countries, levels of care, and illness severity. We will conduct sensitivity and scenario analysis to determine how unit costs vary across scenarios. Conclusions: Results from this study will contribute to the existing body of literature on diarrhea costing and inform future policy decisions related to investments in preventive strategies for Shigella.

Micrometer insights into Nepeta genus: Pollen micromorphology unveiled.

This study provides a comprehensive pollen micromorphology within the Nepeta genus, revealing intricate details about the pollen grains' structure and characteristics. The findings shed light on the evolutionary and taxonomical aspects of this plant genus, offering valuable insights for botanists and researchers studying Nepeta species. The pollen grains of 18 Nepeta species were studied using scanning electron microscopy (SEM) and light microscopy (LM) in Northern Pakistan. At the microscale, pollen quantitative measurements, qualitative traits, and diverse sculpturing patterns were reported and compared. Significant differences in pollen size, shape, ornamentation, and sculpturing patterns were discovered among the Nepeta species. Our data show that exine sculpturing is quite diverse, with most species exhibiting a reticulate perforate pollen pattern. Nepeta connata, Nepeta discolor, Nepeta elliptica, revealed a distinct bireticulate perforate exine stratification. Hexazonocolpate pollen is the most common. Furthermore, the surface membrane attributes of the colpus varied greatly, ranging from rough, scabrate, psilate, to sinuate patterns. Principal Component Analysis (PCA) was used to discover the key factors influencing pollen diversity. PCA results showed that polar and equatorial diameters, colpi size, and exine thickness were the most influential pollen features between Nepeta species. This study adds to our understanding of pollen morphology in the Nepeta genus, offering information on the vast range of characteristics found in this economically important group. The extensive characterization of pollen features provides useful insights for the categorization and differentiation of Nepeta species, adding to the Lamiaceae micromorphology.

Severity of Adverse Effects of Sinovac COVID-19 Vaccine in Postmenopausal Women: A Multicenter Experience From Pakistan.

Introduction The most significant element in halting the coronavirus disease 2019 (COVID-19) epidemic was the availability of reliable and efficient vaccines. Vaccine acceptability is influenced by many factors, including perceptions of the vaccine's safety and side effects. Adverse reactions to vaccines can vary with regard to the type, although they are frequently mild, localized, temporary, and self-limiting. Therefore, this study aimed to assess the prevalence of side effects experienced by postmenopausal women after receiving the Sinovac vaccine. Methods This multicenter, prospective cross-sectional study was carried out at multiple centers in Karachi, Pakistan. In this study, the non-probability sampling method was used. The study continued from August 1, 2022, to January 31, 2023, for six months. The study comprised 600 postmenopausal women over the age of 50 years who received two doses of Sinovac COVID-19 vaccination. Demographic parameters such as gender, the existence of comorbidities, and local and systemic side effects in postmenopausal women were documented as frequencies and percentages. Age, weight, and duration of comorbidities are expressed as means and standard deviations. Results The study findings showed that the mean age of study participants was 63.93 ± 8.24 years. There were related comorbidities with hypertension and diabetes mellitus in 181 (30.2%) and 40 (6.7%) women, respectively. Fever was the most often reported side effect, with 349 (58.2%) participants reporting it and 198 (56.7%) participants reporting it as mild. After the second dose, 234 (39.5%) participants reported fever as their most frequent adverse effect, and 158 (67.5%) of them reported it was mild. Conclusion This study concluded that the most commonly reported side effects among postmenopausal women were fever, pain, and swelling at the injection site after getting either dose of Sinovac vaccine. These overall side effects were generally mild to moderate in intensity, not life-threatening, and did not require hospitalization, although fever was reported in severe intensity in some cases, particularly after the first dose.

Intussusception risk following oral monovalent rotavirus vaccination in 3 Asian countries: A self-control case series evaluation.

Rotavirus vaccines have substantially decreased rotavirus hospitalizations in countries where they have been implemented. In some high- and middle-income countries, a low-level of increased risk of intussusception, a type of acute bowel obstruction, has been detected following rotavirus vaccination. However, no increased risk of intussusception was found in India, South Africa, or a network of 7 other African countries. We assessed the association between a 2-dose monovalent rotavirus vaccine (Rotarix) and intussusception in 3 early-adopter low-income Asian countries -- Afghanistan, Myanmar, and Pakistan. Children <12 months of age admitted to a sentinel surveillance hospital with Brighton level 1 intussusception were eligible for enrollment. We collected information about each child's vaccination status and used the self-controlled case series method to calculate the relative incidence of intussusception 1-7 days, 8-21 days, and 1-21 days following each dose of vaccine and derived confidence intervals with bootstrapping. Of the 585 children meeting the analytic criteria, the median age at intussusception symptom onset was 24 weeks (IQR: 19-29). Overall, 494 (84 %) children received the first Rotarix dose and 398 (68 %) received the second dose. There was no increased intussusception risk during any of the risk periods following the first (1-7 days: 1.01 (95 %CI: 0.39, 2.60); 8-21 days: 1.37 (95 %CI: 0.81, 2.32); 1-21 days: 1.28 (95 %CI: 0.78, 2.11)) or second (1-7 days: 0.81 (95 %CI: 0.42, 1.54); 8-21 days: 0.77 (95 %CI: 0.53, 1.16); 1-21 days: 0.78 (95 %CI: 0.53, 1.16)) rotavirus vaccine dose. Our findings are consistent with other data showing no increased intussusception risk with rotavirus vaccination in low-income countries and add to the growing body of evidence demonstrating safety of rotavirus vaccines.

Typification and Nomenclature Notes on Twenty-Nine Names in Asparagus (Asparagaceae).

Nomenclatural types for twenty-nine names belonging to the genus Asparagus are typified and discussed. The following names are lectotypified: A. altiscandens Engl. & Gilg, A. altissimus Munby, A. baumii Engl. & Gilg, A. benguellensis Baker, A. burchellii Baker, A. curillus Buch.-Ham. ex Roxb., A. deflexus Baker, A. duchesnei L.Linden, A. equisetoides Welw. ex Baker, A. fasciculatus Thunb., A. griffithii, Baker, A. homblei De Wild., A. kaessneri De Wild., A. lecardii De Wild., A. longicladus N.E.Br., A. longiflorus Franch., A. monophyllus Baker, A. palaestinus Baker, A. pastorianus Webb & Berthel., A. persicus Baker, A. poissonii H.Perrier, A. psilurus Welw. ex Baker, A. ritschardii De Wild., A. sapinii De Wild., A. scandens Thunb., A. schumanianus Schltr. ex H.Perrier, A. stellatus Baker, A. subfalcatus De Wild., and A. undulatus (L.f.) Thunb. (synonym of Dracaena undulata L.f.). A new name, Asparagus neofasciculatus, is proposed as a replacement name for A. fasciculatus Thunb., which is an illegitimate later homonym of A. fasciculatus R.Br. The original protologue of these names and the original materials are evaluated. Nomenclature remarks discussing the selection of type specimens are given for each name, and known isotypes or isolectotypes are also cited. This information could be utilized as a reference for future taxonomic and systematic studies on Asparagus around the world.

Micromorphological Sculptural Diversity in Foliar Epidermis and Trichomes Features among Invasive Species.

This study examines the role of light microscopic (LM) and scanning electron microscopic (SEM) micromorphological traits of the epidermis in identifying and classifying invasive plants. SEM was conducted to increase our understanding of microscopic qualities that are not visible in light microscopy and to elucidate unclear affinities among invasive species. The study examines invasive species' morphological and anatomical characteristics from the Pothohar Plateau of Pakistan for the first time. The results showed that various micromorphological features are very useful for species' accurate identification. Adaxial and abaxial surfaces of leaves showed variations in subsidiary cells, glands, anticlinal wall patterns, stomata, and epidermal cells. Epidermal cell shapes observed were irregular, elongated, rectangular, and polygonal. Epidermal cells having maximum length were calculated in Stellaria media (126.3 µm) on adaxial side. On the abaxial surface, the minimum length was noticed in Eucalyptus camaldulensis (28.5 µm). Both glandular and nonglandular trichomes were examined, ranging from unicellular to multicellular. Most of the investigated specimens of leaves were amphistomatic, while some were hypostomatic, like Alternanthera pungens, Calotropis procera, Cannabis sativa, Lantana camara, and Thevetia peruviana. Leaf epidermal morphology contains numerous useful systematic features for accurate identifications of plant species. The micromorphological attributes under observation provide a standard criterion to the researcher for identifications of invasive flora in future morpho-taxonomic studies.

Palyno-taxonomic approach through light and scanning electron microscopy for the identification of herbaceous Asteraceae species from Sikaram Mountain Pak-Afghan border.

Due to the diverse and rich taxa, Asteraceae needs to explore from the untouched area. In this study, the pollen study was intended to assess the taxonomic value for Asteraceous taxa growing on the Sikaram Mountain Pak-Afghan border. Both microscopic techniques (LM and SEM) play a significant role in the identification and classification of herbaceous species of Asteraceae for their taxonomic and systematic implication. Pollen was observed and measured for the 15 species of Asteraceae. For investigated taxa 15 pollen characters with size, shape, polar view, pollen type, aperture orientation, and exine sculpturing in the eurypalynous pollen. Consequently, the pollen grains are usually Tricolporate, triangular to circular in polar view, while, the shape of pollen varies from subulate, oblate, and prolate along with prolate to spheroidal whereas, sculpturing also varies from Scabrate to micro reticulate, echino-perforate, Scabrate to echinate, echinate to granulate and echinate were observed. Similarly, among the quantitative data minimum values of polar and equatorial were 15.8 ± 0.74 µm in Filago pyramidata and 17.85 ± 0.39 µm in Heteropappus altaicus was measured respectively, while; the length of the spine with the least value of 2.45 ± 0.31 µm in Hertia intermedia and highest with 7.55 ± 0.31 µm was observed in Cirsium wallichii. The Exine thickness is a minimum of 1.70 ± 0.35 µm in Launaea nudicaulis and a maximum of 5.65 ± 3.59 µm in Cirssium vulgare. In addition, the highest pollen fertility (87%) in Centaurea iberica while the highest pollen sterility (32%) was recorded in Cirsium verutum. Furthermore, clustering via UPGMA, PCA, and PCoA analysis was performed for the separation of closely related taxa. It is concluded from this research work that palynological study has a crucial role in taxonomic, pure, and applied sciences. This study can be further authenticated and improved by a Phylogenetic study based on chloroplast DNA analysis & whole genome sequencing. RESEARCH HIGHLIGHTS: Pollen ultrastructure among 15 Asteraceous taxa. Micromorphological attributes measured via LM and SEM. Exine sculpture elements patterns provide accurate identification. Taxonomic keys were developed for its systematics implications.

Membrane-processed honey samples for pollen characterization with health benefits.

Better processing techniques must be utilized widely due to the rising demand for honey. The most common honey processing techniques are applied to melissopalynomorphs to check the quality and quantity of valuable honey using microporous ultrafiltration membranes. It is essential to have the ability to selectively filter out sugars from honey using ultrafiltration. This study authenticated 24 honey samples using membrane reactors ultrafiltration protocol to describe the pollen spectrum of dominant vegetation. The purpose of this study was also to explore nutritional benefits as well as the active phytochemical constituents of honey samples. Honey samples were collected and labeled Acacia, Eucalyptus, and Ziziphus species based on plant resources provided by local beekeepers. A variety of honeybee flora was collected around the apiaries between 2020 and 2021. Honey analysis revealed that the pollen extraction of 24 bee foraging species belonging to 14 families. The honey membrane technology verified the identities of honey and nectar sources. Also, pollen identified using honey ultrafiltration membranes revealed dominant resources: Acacia spp. (69%), Eucalyptus spp. (52%) and Ziziphus spp. Honey filtration using a membrane technology classified 14 samples as unifloral, represented by six dominant pollen types. The absolute pollen count in the honey sample revealed that 58.33% (n = 14) belong to Maurizio's class I. Scanning ultrasculpturing showed diverse exine patterns: reticulate, psilate, scabrate-verrucate, scabrate-gemmate, granulate, perforate, microechinate, microreticulate, and regulate to fossulate for correct identification of honey pollen types. Honey ultrafiltration should be utilized to validate the botanical sources of honey and trace their biogeographic authenticity. Thus, it is imperative to look at the alternative useful method to identify the botanical origin of filtered honey. It is critical to separate honey from adulteration by a standardized protocol. Membrane technology has yielded significant outcomes in the purification of honey.

Efficient application of newly synthesized green Bi2O3 nanoparticles for sustainable biodiesel production via membrane reactor.

Introduction of waste and non-edible oil seeds coupled with green nanotechnology offered a pushover to sustainable and economical biofuels and bio refinery production globally. The current study encompasses the synthesis and application of novel green, highly reactive and recyclable bismuth oxide nanocatalyst derived from Euphorbia royealeana (Falc.) Boiss. leaves extract via biological method for sustainable biofuel synthesis from highly potent Cannabis sativa seed oil (34% w/w) via membrane reactors. Advanced techniques such as X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Diffraction X-Ray (EDX), and FT-IR were employed to illustrate the newly synthesized green bismuth oxide nanoparticles. 92% of FAMEs were produced under optimal reaction conditions such as a 1.5% w/w catalyst weight, 1:12 oil to methanol molar ratio, and a reaction temperature of 92 ⸰C for 3.5 h via membrane reactor. The synthesized Cannabis biodiesel was identified using the FT-IR and GC-MS techniques. The fuel properties of synthesized biofuels (acid number 0.203 mg KOH/g, density 0.8623 kg/L, kinematic viscosity 5.32 cSt, flash point 80 °C, pour point -11 °C, cloud point -11 °C, and Sulfur 0.00047 wt %, and carbon residues 0.2) were studied and established to be comparable with internationally set parameters. The experimental data (R2 = 0.997) shows that this reaction follow pseudo first-order kinetics. These findings affirm the application of green bismuth oxide nanoparticles as economical, highly reactive and eco-friendly candidate for industrial scale biodiesel production from non-edible oil seeds.

Tendril Anatomy: A Tool for Correct Identification among Cucurbitaceous Taxa.

This research examined the histological micro-structure of tendril vasculature in cucurbitaceous taxa. In this research, the tendril anatomy of 17 taxa of Cucurbitaceae categorized into seven genera, including Cucumis (five species), Cucurbita and Luffa (three species each), Citrullus and Momordica (two species each) while Lagenaria and Praecitrullus (one species each), collected from different areas of the Thal desert were examined via microscopic imaging to explore its taxonomic significance. Tendril transverse sections were cut with a Shandon Microtome to prepare slides. The distinctive characteristics of taxonomic value (qualitative and quantitative) include tendril and vascular bundle shape, variation in the number of vascular bundles, tendril diameter length, layers of sclerenchyma, and shape of collenchyma and epidermal cells. Tendril shapes observed are irregular, slightly oval-shaped, slightly C shaped, angular (4-angled, 6-angled, or polygonal), and star shaped. Quantitative measurements were taken to analyze the data statistically using SPSS software. Cucurbita pepo had a maximum tendril diameter length of 656.1 µm and a minimum in Momordica balsamina of 123.05 µm. The highest number of vascular bundles (12) were noticed in Luffa acutangula var.amara. Angular type was prominent in collenchyma, and irregular shape was dominant in sclerenchyma cells. A maximum of seven to nine sclerenchyma layers were present in Lagenaria siceraria and a minimum of two or three layers in Cucumis melo subsp. agrestis, Cucumis melo var. flexuosus, and Cucumis melo var.cantalupensis. Epidermis cells also show great variations with a rectangular shape being dominant. Statistical UPGMA dendrogram clustering of tendril vasculature traits shows that histological sections studied with microscopic techniques can be used to identify species and will play a vital role in future taxonomic and phylogenic linkages.

Membrane reactor based synthesis of biodiesel from Toona ciliata seed oil using barium oxide nano catalyst.

Membrane technology has been embraced as a feasible and promising substitute to the traditional technologies employed for biodiesel synthesis which are energy and time consuming. It needs less energy, has high stability, is environmentally friendly, and is simple to operate and control. Therefore, in our current study membrane technology was employed to synthesize biodiesel from Toona ciliate novel and non-edible seed oil. Since Toona ciliata has affluent oil content (33.8%) and is effortlessly and extensively available. In fact, we intended to scrutinize the effects of green synthesized barium oxide nanoparticles for one step transesterification of biodiesel production using membrane technology followed by characterization of prepared catalyst via innovative techniques. Optimal yield of biodiesel attained was 94% at 90 °C for 150 min with methanol to oil molar ratio of 9:1 and amount of about 0.39 wt %. Quantitative analysis of synthesized Toona ciliata oil biodiesel was carried out by advance techniques of Gas chromatography mass spectrometry (GC-MS), Fourier-transform infrared (FTIR) spectroscopy and Nuclear magnetic resonance (NMR) which authorize the synthesis of fatty acid methyl ester compounds using oil from Toona ciliata seeds. Values of Toona ciliata fuel properties for instance flash point (70°C), density (0.89 kg/m3), viscosity (5.25 mm2/s), cloud point (-8°C) and pour point (-11°C) met the specifications of international standards i. e American (ASTM D-6751), European (EN-14214) and China (GB/T 20,828). Subsequently, it is concluded that membrane technology is environmentally friendly and efficient technique for mass-production of sustainable biodiesel using green nano catalyst of barium oxide.

Seed Coat Morphology and Sculpturing of Selected Invasive Alien Plants from Lesser Himalaya Pakistan and Their Systematic Implications.

Invasive alien species (IAS) are considered as the second major threat to biodiversity after habitat destruction worldwide. They are aggressive competitors and dominate an ecosystem where they introduce and cause reduction in indigenous diversity. Invasive plants alter the evolutionary pathways of native species by competition, niche displacement, hybridization, introgression, predation, and ultimately extinction of native species. Biological invasion also results in economic and environmental damage and harm to human health. Invasive plants have an effective reproductive as well as dispersal mechanisms. Most invasive plants produce abundant fruits and seeds that are widely disseminated and remain viable in the soil for several years. Invasive plants may change their seed character in order to adapt themselves to the new environment and facilitate their performance. A study on seed coat sculpturing in invasive alien plants collected from Lesser Himalaya region, Pakistan, was conducted using scanning electron microscope to determine the importance of seed morphological characters as an additional tool for identification. Quantitative characters such as seed length and width, macromorphological characters including color, hilum position, and seed shape, and micromorphological characters of seed including surface patterns and periclinal and anticlinal wall of seeds were studied. Findings at the present indicate that most of the seeds were found spherical followed by ovate and elliptical in shape with smooth surface and showed terminal hilum. Almost reticulate seed patterns were observed in seeds. Majority of seeds showed raised anticlinal walls with protuberance periclinal walls. The seeds of Xanthium strumarium were observed with maximum length of 13 mm and with width of 8 mm. Length by width ratio of seeds was also calculated; it was found that maximum L/W ratio was observed in Sonchus oleraceus L., i.e., 2.66. Seed characters, both macro- and micromorphological, furnish useful data for classification and delimitation of invasive taxa. This study will help to understand the invasion mechanism in plants due to variations in seed surface, shape, and other characters. Adaptive behavior of the seed during the invasion process of the new ecosystem is also elaborated.

Field evaluation of typhoid conjugate vaccine in a catch-up campaign among children aged 9 months to 15 years in Sindh, Pakistan.

BACKGROUND: Typhoid conjugate vaccine (TCV) has recently been introduced in the expanded program for immunization (EPI) in Pakistan. Before its introduction in routine immunization, a onetime catchup campaign among children 9 months to 15 years old was conducted in November 2019. We performed field evaluation of TCV against culture confirmed Salmonella Typhi (S. Typhi) among 9 months to 15 years old children during the catch up campaign in Karachi and Hyderabad. METHODS: A rapid assessment of blood culture confirmed S. Typhi was performed. Age eligible cases of culture confirmed S. Typhi were identified from the laboratory networks of Aga Khan University Hospital Karachi and Hyderabad, Kharadar General Hospital Karachi, and Liaqat University of Medical & Health Sciences (LUMHS) Hyderabad. Information on sociodemographic, typhoid vaccination history and antimicrobial resistance was collected using a structured questionnaire. Patient medical records and lab reports were also reviewed to collect information on diagnosis and antimicrobial susceptibility information. Information about the population vaccination coverage during catch-up campaign was obtained from the provincial EPI office. Field performance of TCV in catchup campaign was measured by calculating the effectiveness using rapid screening method which is less resource-intensive technique of calculating vaccine effectiveness (VE). RESULTS: Overall, 968 culture confirmed typhoid cases were enrolled. Among them, 82% (793/968) were from Karachi and 18% (175/968) from Hyderabad. The average age of the participants was 5.68 years, and 54% (523/968) were male. 6% (62/968) of the culture confirmed S. Typhi cases were multidrug resistant (MDR), and 61% (586/968) were extensively drug resistant (XDR). The VE using the TCV coverage data provided by EPI was 98%. CONCLUSION: TCV is effective against culture confirmed S. Typhi among children aged 9 months to 15 years in the catch-up campaign setting. While typhoid vaccination can significantly decrease the burden of typhoid disease, improvements in sanitation and hygiene are necessary for the prevention of spread of enteric fever. Longer term follow up will be needed to assess the duration of protection and requirement for booster doses of TCV.